Ammonium transport in Arabidopsis thaliana:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2001
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 112 S. Ill., graph. Darst. : 21 cm |
Internformat
MARC
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| 100 | 1 | |a Sohlenkamp, Christian |d 1971- |e Verfasser |0 (DE-588)123381584 |4 aut | |
| 245 | 1 | 0 | |a Ammonium transport in Arabidopsis thaliana |c vorgelegt von Christian Sohlenkamp |
| 264 | 1 | |c 2001 | |
| 300 | |a 112 S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Berlin, Techn. Univ., Diss., 2001 | ||
| 650 | 0 | 7 | |a Carrier-Proteine |0 (DE-588)4213127-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Ammoniumverbindungen |0 (DE-588)4142226-0 |2 gnd |9 rswk-swf |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009658270 | |
Datensatz im Suchindex
| _version_ | 1807229173123514368 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
CONTENT
ZUSAMMENFASSUNG.
.
._
._
.1
ABBREVIATIONS.?
1
INTRODUCTION
.9
1.1
PLANT
BIOTECHNOLOGY
.
9
1.2
AMMONIUM
IN
SOIL/NITROGEN-CYCLE
.
9
1.3
IMPORTANCE
OF
N-FERTILISER
.
11
1.4
ASSIMILATION
OF
INORGANIC
NITROGEN
IN
PLANTS
.
12
1.4.1
PRIMARY
NITROGEN
ASSIMILATION
.
12
1.4.2
REASSIMILATION
OF
PHOTORESPIRATORY
AMMONIUM
.
13
1.4.3
REASSIMILATION
OF
RECYCLED
AMMONIUM
.
15
1.5
AMMONIUM
TRANSPORTERS
.
15
1
.6
STRATEGIES
TO
IMPROVE
N-UPTAKE
/
EFFICIENCY
IN
PLANTS
.
16
1.6.1
TRANSFER
OF
NITROGENASE
GENES
TO
PLANTS
.
16
1.6.2
ESTABLISHMENT
OF
NEW
SYMBIOSES
.
17
1.6.3
MORE
EFFICIENT
UPTAKE
OF
NITROGEN
AND
NITROGEN
ASSIMILATION
.
17
1.7
AIM
OF
THIS
WORK
.
18
2
MATERIALS
AND
METHODS
.20
2.1
MATERIALS
.
20
2.1.1
ENZYMES,
CHEMICALS
AND
EQUIPMENT
.
20
2.1.2
SYNTHETIC
OLIGONUCLEOTIDES
.
21
2.1.3
CDNA
LIBRARY
.
23
2.1.4
PLASMIDS
.
23
2.1.5
BACTERIAL
AND
YEAST
STRAINS
.
24
2.1.6
PLANTS
.
24
2.1.7
MEDIA
.
24
TABLE
OF
CONTENTS
2.2
METHODS
.
26
2.2.1
DNA
MANIPULATIONS
.
26
2.2.1.1
CLONING
AND
DNA
SEQUENCE
ANALYSIS
.
26
2.2.1.2
SCREENING
OF
A
XZIPLOX
CDNA
LIBRARY
.
26
2.2.1.3
SOUTHERN
BLOT
ANALYSIS
.
26
2.2.2
RNA
MANIPULATIONS
.
27
2.2.2.1
RNA
ISOLATION
.
27
2.2.2.2
RNA
BLOT
ANALYSIS
.
27
2.2.3
TRANSFORMATIONS
.
28
2.2.3.1
TRANSFORMATION
OF
BACTERIA
.
28
2.2.3.2
TRANSFORMATION
OF
YEASTS
.
28
2.2.3.3
TRANSFORMATION
OF
PLANTS
.
28
2.2.4
PHYSIOLOGICAL
STUDIES
OF
YEAST
CELLS
.
28
2.2.4.1
COMPLEMENTATION
OF
MLY131A/A-GROWTH
ON
METHYLANUNONIUM
.
28
2.2.4.2
GROWTH
KINETICS
.
28
2.2.4.3
AMMONIUM
DEPLETION
ASSAY
.
29
2.2.4.4
INHIBITOR
STUDIES
.
29
2.2.5
CHARACTERISATION
OF
TRANSGENIC
ARABIDOPSIS
THALIANA
EXPRESSING
AN
AMT2
HAIRPIN
CONSTRUCT
.
29
2.2.5.1
CLONING
OF
THE
HAIRPIN
CONSTRUCT
.
29
2.2.5.2
ANALYSIS
OF
TRANSGENIC
ARABIDOPSIS
THALIANA
EXPRESSING
THE
AMT2
HAIRPIN
CONSTRUCT
.
30
2.2.6
HISTOCHEMICAL
ANALYSIS
OF
PROMOTER-GUS
FUSIONS
.
30
2.2.7
DETERMINATION
OF
MRNA
TRANSCRIPT
LEVELS
USING
REAL-TIME
RT-PCR
.
31
2.2.8
TRANSIENT
EXPRESSION
OF
GFP
FUSIONS
IN
LEAVES
OF
ARABIDOPSIS
THALIANA
.
32
2.2.8.1
CONSTRUCTION
OF
TRANSLATIONAL
FUSIONS
BETWEEN
AMMONIUM
TRANSPORTERS
AND
THE
REPORTER
PROTEIN
GFP
.
32
2.2.8.2
PREPARATION
OF
GOLD
PARTICLES
.
33
4
TABLE
OF
CONTENTS
2.2.83
PARTICLE
BOMBARDMENT
.
33
2.2.8.4
CONFOCAL
MICROSCOPY
.
33
3
RESULTS
.
.34
3.1
ISOLATION
AND
CHARACTERISATION
OF
ATAMT2
.
34
3.1.1
IDENTIFICATION
OF
THE
ATAMT2
SEQUENCE
.
34
3.1.2
PCR
AMPLIFICATION
AND
CLONING
OF
ATAMT2
.
34
3.1.3
SEQUENCE
ANALYSIS
OF
AMT2
.
34
3.1.4
SOUTHERN
BLOT
ANALYSIS
.
38
3.1.5
HETEROLOGOUS
EXPRESSION
OF
AMT2
IN
SACCHAROMYCES
CEREVISIAE
.
38
3.1.5.1
COMPLEMENTATION
OF
MLY131A/A
.
39
3.1.5.2
GROWTH
ON
METHYLAMMONIUM/UPTAKE
OF
METHYLAMMONIUM
.
41
3.1.5.3
UPTAKE
OF
AMMONIUM
BY
THE
COMPLEMENTED
YEAST
MUTANT
.
41
3.1.5.4
INHIBITION
OF
AMMONIUM
UPTAKE
BY
MONOVALENT
CATIONS
.
43
3.1.5.5
INHIBITION
OF
AMMONIUM
UPTAKE
BY
IONOPHORES/PROTONOPHORES
.
43
3.1.6
DETERMINATION
OF
A
K
M
-VALUE
OF
AMT2
FOR
AMMONIUM
.
44
3.1.7
EXPRESSION
ANALYSIS
OF
AMT2
.
44
3.1.7.1
INFLUENCE
OF
DIFFERENT
NITROGEN
REGIMES
ON
THE
TRANSCRIPTION
OF
AMT2
.
45
3.1.7.2
TRANSCRIPTION
OF
AMT2
DURING
THE
COURSE
OF
A
DAY
.
47
3.1.7.3
ORGAN
SPECIFIC
EXPRESSION
OF
AMT2
.
49
3.1.7.4
INFLUENCE
OF
CO2
ON
AMT2
TRANSCRIPTION
.
50
3.2
EFFECTS
OF
THE
INHIBITION
OF
AMT2
EXPRESSION
IN
PLANTA
.
51
3.2.1
SCREENING
OF
TRANSGENIC
PLANTS
EXPRESSING
THE
AMT2
HAIRPIN
CONSTRUCT
.
52
3.2.2
GROWTH
OF
TRANSGENIC
PLANTS
EXPRESSING
THE
AMT2
HAIRPIN
CONSTRUCT
UNDER
DIFFERENT
NUTRITIONAL
REGIMES
.
54
3.3
CHARACTERISATION
OF
THE
AMT
SUPER-FAMILY
.
59
3.3.1
IN
SILICO
ANALYSIS
OF
AMT
PROMOTERS
AND
CODING
SEQUENCES
.
59
3.3.2
QUANTIFICATION
OF
AMMONIUM
TRANSPORTER
TRANSCRIPT
LEVELS
USING
REAL-TIME
RT
PCR
.
62
5
TABLE
OF
CONTENTS
3.3.3
DETERMINATION
OF
THE
EXPRESSION
PATTERNS
OF
THE
DIFFERENT
AMMONIUM
TRANSPORTER
GENES
IN
PLANTA
USING
PROMOTER-GUS
FUSIONS
.
68
3.3.4
SUBCELLULAR
LOCALISATION
OF
DIFFERENT
AMMONIUM
TRANSPORTERS
BY
TRANSIENT
EXPRESSION
OF
TRANSLATIONAL
TRANSPORTER-GFP
FUSIONS
IN
ARABIDOPSIS
LEAVES
71
4
DISCUSSION
.
77
4.1
THE
PHYSIOLOGY
OF
AMMONIUM
TRANSPORT
IN
PLANTS
.
77
4.2
CHARACTERISATION
OF
AMT2
.
79
4.2.1
CLONING
AND
SEQUENCE
ANALYSIS
OF
AMT2
.
79
4.2.2
ANALYSIS
OF
AMT2
GENE
TRANSCRIPTION
.
80
4.2.3
SPECIFICITY
OF
AMT2
.
83
4.2.4
AMT2
IN
OTHER
PLANTS
.
85
4.3
INHIBITION
OF
AMT2
ACTIVITY
IN
PLANTA
USING
RNAI
.
86
4.4
THE
AMT
SUPER-FAMILY
IN
PLANTS
.
88
4.4.1
EXPRESSION
ANALYSIS
USING
PROMOTER
GUS
FUSIONS
AND
REAL-TIME
RT-PCR
.
89
4.4.2
SUBCELLULAR
LOCALISATION
OF
AMMONIUM
TRANSPORTERS
BY
TRANSIENT
EXPRESSION
OF
TRANSPORTER
GFP
FUSIONS
.
93
5
SUMMARY
AND
CONCLUSIONS
.
95
6
REFERENCES
.
98
6 |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV014098501 |
| illustrated | Illustrated |
| indexdate | 2024-08-13T00:14:04Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009658270 |
| oclc_num | 248419672 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-29T DE-634 DE-83 |
| owner_facet | DE-91 DE-BY-TUM DE-29T DE-634 DE-83 |
| physical | 112 S. Ill., graph. Darst. : 21 cm |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| record_format | marc |
| spelling | Sohlenkamp, Christian 1971- Verfasser (DE-588)123381584 aut Ammonium transport in Arabidopsis thaliana vorgelegt von Christian Sohlenkamp 2001 112 S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Berlin, Techn. Univ., Diss., 2001 Carrier-Proteine (DE-588)4213127-3 gnd rswk-swf Ammoniumverbindungen (DE-588)4142226-0 gnd rswk-swf Ackerschmalwand (DE-588)4141299-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ackerschmalwand (DE-588)4141299-0 s Ammoniumverbindungen (DE-588)4142226-0 s Carrier-Proteine (DE-588)4213127-3 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009658270&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sohlenkamp, Christian 1971- Ammonium transport in Arabidopsis thaliana Carrier-Proteine (DE-588)4213127-3 gnd Ammoniumverbindungen (DE-588)4142226-0 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| subject_GND | (DE-588)4213127-3 (DE-588)4142226-0 (DE-588)4141299-0 (DE-588)4113937-9 |
| title | Ammonium transport in Arabidopsis thaliana |
| title_auth | Ammonium transport in Arabidopsis thaliana |
| title_exact_search | Ammonium transport in Arabidopsis thaliana |
| title_full | Ammonium transport in Arabidopsis thaliana vorgelegt von Christian Sohlenkamp |
| title_fullStr | Ammonium transport in Arabidopsis thaliana vorgelegt von Christian Sohlenkamp |
| title_full_unstemmed | Ammonium transport in Arabidopsis thaliana vorgelegt von Christian Sohlenkamp |
| title_short | Ammonium transport in Arabidopsis thaliana |
| title_sort | ammonium transport in arabidopsis thaliana |
| topic | Carrier-Proteine (DE-588)4213127-3 gnd Ammoniumverbindungen (DE-588)4142226-0 gnd Ackerschmalwand (DE-588)4141299-0 gnd |
| topic_facet | Carrier-Proteine Ammoniumverbindungen Ackerschmalwand Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009658270&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT sohlenkampchristian ammoniumtransportinarabidopsisthaliana |