Study of genetic relationships of avocado (Persea americana Mill.) and related types from Coasta Rica using molecular markers:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Beuren ; Stuttgart
Grauer
2000
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: Hohenheim, Univ., Diss., 2000. - Zsfassung in dt. Sprache |
| Beschreibung: | V, 125 S. Ill., graph. Darst., Kt. : 21 cm |
| ISBN: | 3861863332 |
Internformat
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Datensatz im Suchindex
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Titel: Study of genetic relationships of Avocado (Persea americana Mill.) and related types from Costa Rica
Autor: Yousef, Amani Adnan
Jahr: 2000
CONTENTS
CONTENTS
1. Introduction.1
1.1. Avocado.1
1.2. Avocado taxonomy.1
1.3. Numerical taxonomy.7
1.4. Application of molecular markers in genetic relationship studies of
Plants.7
1.4.1. Random amplified polymorphic DNA (RAPD).9
1.4.2. Restriction site analysis of the chloroplast DNA (cpDNA).10
1.4.2.1. Chloroplast DNA.10
1.4.2.2. Restriction site variation in cpDNA.10
1.4.3. Ribosomal DNA (rDNA).11
1.4.3.1. Non transcribed spacer of 5S rDNA.13
1.4.3.2. Internal transcribed spacer of 18S-25S rDNA.13
1.4.3.3. Intergenic spacer of 18S-25S rDNA.14
1.4.3.4. DNA sequencing.15
1.4.3.4.1. Cycle sequencing.16
1.5. The aim of the study.17
2. Materials and Methods.19
2.1. Plant material.19
2.2. DNA extraction methods .19
2.2.1. DNA extraction with the modified Thomson Henry (1995)
method.19
2.2.2. DNA isolation using silica-gel based membranes: with DNeasy
spin colums (Q1AGEN Plant Mini Kit).22
2.3. RARD assay.24
2.3.1. DNA amplification.24
2.3.2. Gel-electrophoresis.24
2.3.3. Data analysis.24
2.4. Restriction site analysis of the chloroplast DNA.25
2.4.1. DNA isolation.25
2.4.2. DNA amplification.25
2.4.3. RFLP Protocol.27
2.5. Ribosomal DNA.29
2.5.1. Non-transcribed spacer of 5S rDNA.29
2.5.1.1. DNA amplification.29
2.5.1.2. Gel-electrophoresis.29
CONTENTS
2.5.2. Internal transcribed spacer of 18S-25S rDNA .30
2.5.2.1. Optimization of the reaction conditions.30
2.5.2.2. Gel-electrophoresis.31
2.5.2.3. Data analysis.31
2.5.3. Intergenic spacer of 18S- 25S rDNA.32
2.5.3.1. Optimization of the reaction conditions.32
2.5.3.1.1. Primer selection and design.32
2.5.3.1.2. DNA polymerases.33
2.5.3.1.3. Temperature profile.33
2.5.3.2. Gel-electrophoresis.33
2.5.4. DNA sequencing of the intergenic spacer of 18S-25S rDNA .36
2.5.4.1. Isolation and purification of DNA from the agarose
gel (using QIAGEN gel extraction kit).36
2.5.4.2. Primer.37
2.5.4.3. Sequencing assay.37
2.5.4.4. Automated Laser Fluorescent (ALF) sequencer.38
2.5.4.5. Sequence alignment and data analysis.38
3. Results.39
3.1. DNA isolation.39
3.1.1. Estimation of DNA quantity.39
3.1.2. Presence or absence of anise scent.39
3.2. RARD assay.40
3.2.1. Production of polymorphic fragments.40
3.2.2. Race-specific markers.40
3.2.3. UPGMA dendrogram and similarity matrix.49
3.3. Restriction site analysis of the chloroplast DNA.53
3.4. Ribosomal DNA .55
3.4.1. NTS.55
3.4.2. ITS.57
3.4.3. IGS.61
3.4.3.1. DNA sequence of IGS.65
4. Discussion .76
4.1. DNA isolation.76
4.2. Genetic relationships within the cultivated avocado.77
4.3. Genetic diversity of Costa Rica avocado "Aguacate de Monte" and
their possible classification.81
4.4. Genetic relationships between the cultivated avocado and Costa Rica
avocado.86
CONTENTS III
4.5. Utility of molecular markers to study the genetic relationships of
avocado.89
4.5.1. RAPD.89
4.5.2. Restriction analysis of cpDNA.90
4.6. Ribosomal DNA as a tool for screening variation.91
4.6.1. NTS.92
4.6.2. ITS.94
4.6.3. IGS.96
5. Conclusion.101
6. References.104
7. Abstract.120
8. Zusammenfassung.123 |
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| spelling | Yousef, Amani Adnan Verfasser aut Study of genetic relationships of avocado (Persea americana Mill.) and related types from Coasta Rica using molecular markers Amani Adnan Yousef Beuren ; Stuttgart Grauer 2000 V, 125 S. Ill., graph. Darst., Kt. : 21 cm txt rdacontent n rdamedia nc rdacarrier Zugl.: Hohenheim, Univ., Diss., 2000. - Zsfassung in dt. Sprache Genetische Variabilität (DE-588)4264352-1 gnd rswk-swf Markierungsgen (DE-588)4168918-5 gnd rswk-swf Avocado (DE-588)4120964-3 gnd rswk-swf Costa Rica (DE-588)4010618-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Costa Rica (DE-588)4010618-4 g Avocado (DE-588)4120964-3 s Genetische Variabilität (DE-588)4264352-1 s Markierungsgen (DE-588)4168918-5 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009603783&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Yousef, Amani Adnan Study of genetic relationships of avocado (Persea americana Mill.) and related types from Coasta Rica using molecular markers Genetische Variabilität (DE-588)4264352-1 gnd Markierungsgen (DE-588)4168918-5 gnd Avocado (DE-588)4120964-3 gnd |
| subject_GND | (DE-588)4264352-1 (DE-588)4168918-5 (DE-588)4120964-3 (DE-588)4010618-4 (DE-588)4113937-9 |
| title | Study of genetic relationships of avocado (Persea americana Mill.) and related types from Coasta Rica using molecular markers |
| title_auth | Study of genetic relationships of avocado (Persea americana Mill.) and related types from Coasta Rica using molecular markers |
| title_exact_search | Study of genetic relationships of avocado (Persea americana Mill.) and related types from Coasta Rica using molecular markers |
| title_full | Study of genetic relationships of avocado (Persea americana Mill.) and related types from Coasta Rica using molecular markers Amani Adnan Yousef |
| title_fullStr | Study of genetic relationships of avocado (Persea americana Mill.) and related types from Coasta Rica using molecular markers Amani Adnan Yousef |
| title_full_unstemmed | Study of genetic relationships of avocado (Persea americana Mill.) and related types from Coasta Rica using molecular markers Amani Adnan Yousef |
| title_short | Study of genetic relationships of avocado (Persea americana Mill.) and related types from Coasta Rica using molecular markers |
| title_sort | study of genetic relationships of avocado persea americana mill and related types from coasta rica using molecular markers |
| topic | Genetische Variabilität (DE-588)4264352-1 gnd Markierungsgen (DE-588)4168918-5 gnd Avocado (DE-588)4120964-3 gnd |
| topic_facet | Genetische Variabilität Markierungsgen Avocado Costa Rica Hochschulschrift |
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