Quantitative PCR protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Pr.
1999
|
| Schriftenreihe: | Methods in molecular medicine
26 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 304 S. graph. Darst. |
| ISBN: | 0896035182 |
Internformat
MARC
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| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a DNA |x analysis | |
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Datensatz im Suchindex
| _version_ | 1804128867725606912 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
Part I. Reviews
1 Quantitative PCR: A Survey of the Present Technology
Udo Reischl and Bernd Kochanowski 3
2 General Principles of Quantitative PCR
Luc Raeymaekers 31
3 Effects of Collection, Processing, and Storage on RNA Detection
and Quantification
Mark Holodniy 43
4 Quantitative RT PCR
Paul D. Siebert 61
5 Kinetic Quantitative PCR vs End Point Quantitative PCR
with Internal Standard
Olivier Lantz, Elizabeth Bonney, Franck Griscelli,
and Yassine Taoufik 87
6 Comparison of Competitive PCR and Positive Control Based PCR
Frangois Mallet 103
Part II. Protocols
7 End Point Titration PCR for Quantitation of Cytomegalovirus DNA
Jerzy K. Kulski 119
8 Analysis of Amplified DNA Molecules by Capillary Electrophoresis
and Laser Induced Fluorescence
Michael J. Fasco 131
9 Competitor Calibration and Analysis of Competitive Amplified PCR
Products by High Performance Liquid Chromatography (HPLC)
Thomas Kohler 147
10 Quantifying Amplicons with ELISA
Olivier Lantz, Elizabeth Bonney, and Yassine Taoufik 157
vii
viii Contents
11 Competitive PCR Quantitation Utilizing a Microtiter Plate Based
Format for the Detection of PCR Products
Bernd Kochanowski and Wolfgang Jilg 171
12 Competitive and Differential RT PCR (CD RT PCR) for Measurement
of Normalized Gene Expression Using Antisense Competitors
Eric de Kant 183
13 Amplified Assay for Specific Dual Labeled DNA Using
the Coagulation Cascade (EDNA ELCA)
George J. Doellgast, G. Alan Beard, Margaret Sheehan, Nathan
Iyer, Louis S. Kucera, and Stephen H. Richardson 197
14 Quantitative PCR with Internal Standardization and OLA ELISA
Product Analysis for the p53 Tumor Suppressor Gene
Meinhard Hahn and Alfred Pingoud 217
15 Quantitative Analysis of Human DNA Sequences by PCR
and Solid Phase Minisequencing
Anu Suomalainen and Ann Christine Syvanen 233
16 High Resolution PCR Quantitation by AmpliSensor Assay
Chang Ning Wang 245
17 Construction of Polycompetitors for Competitive PCR
David B. Corry and Richard M. Locksley 253
18 Tailed RT PCR for the Quantitation of Chloramphenicol Acetyl
Transferase (CAT)mRNA
MarlyseC. Knuchel and Aftab A. Ansari 265
19 A Stochastic PCR Approach for RNA Quantification
in Multiple Samples
Adrian Puntschart and Michael Vogt 277
20 Quantitation of mRNA Species by RT PCR on Total mRNA
Population with Nonradioactive Probes
Sabine Herblot, Benoft Rousseau, and Jacques Bonnet 289
Index 301
|
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| indexdate | 2024-07-09T18:56:04Z |
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| isbn | 0896035182 |
| language | English |
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| series | Methods in molecular medicine |
| series2 | Methods in molecular medicine |
| spelling | Quantitative PCR protocols ed. by Bernd Kochanowski ... Totowa, NJ Humana Pr. 1999 X, 304 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 26 Polymerase Chain Reaction cabt Polymerase kettingreactie gtt Réaction en chaîne de la polymérase - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten DNA analysis Polymerase Chain Reaction methods Polymerase chain reaction Laboratory manuals RNA analysis Quantitative Analyse (DE-588)4125311-5 gnd rswk-swf Gen (DE-588)4128987-0 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Gen (DE-588)4128987-0 s Quantitative Analyse (DE-588)4125311-5 s Polymerase-Kettenreaktion (DE-588)4256726-9 s DE-604 Kochanowski, Bernd Sonstige oth Methods in molecular medicine 26 (DE-604)BV011334960 26 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009592675&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Quantitative PCR protocols Methods in molecular medicine Polymerase Chain Reaction cabt Polymerase kettingreactie gtt Réaction en chaîne de la polymérase - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten DNA analysis Polymerase Chain Reaction methods Polymerase chain reaction Laboratory manuals RNA analysis Quantitative Analyse (DE-588)4125311-5 gnd Gen (DE-588)4128987-0 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| subject_GND | (DE-588)4125311-5 (DE-588)4128987-0 (DE-588)4256726-9 (DE-588)4143413-4 |
| title | Quantitative PCR protocols |
| title_auth | Quantitative PCR protocols |
| title_exact_search | Quantitative PCR protocols |
| title_full | Quantitative PCR protocols ed. by Bernd Kochanowski ... |
| title_fullStr | Quantitative PCR protocols ed. by Bernd Kochanowski ... |
| title_full_unstemmed | Quantitative PCR protocols ed. by Bernd Kochanowski ... |
| title_short | Quantitative PCR protocols |
| title_sort | quantitative pcr protocols |
| topic | Polymerase Chain Reaction cabt Polymerase kettingreactie gtt Réaction en chaîne de la polymérase - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten DNA analysis Polymerase Chain Reaction methods Polymerase chain reaction Laboratory manuals RNA analysis Quantitative Analyse (DE-588)4125311-5 gnd Gen (DE-588)4128987-0 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| topic_facet | Polymerase Chain Reaction Polymerase kettingreactie Réaction en chaîne de la polymérase - Manuels de laboratoire Wetenschappelijke technieken Wissenschaftliches Arbeiten DNA analysis Polymerase Chain Reaction methods Polymerase chain reaction Laboratory manuals RNA analysis Quantitative Analyse Gen Polymerase-Kettenreaktion Aufsatzsammlung |
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