Gene targeting in embryonic stem cells: = Gene-Targeting in embryonalen Stammzellen
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2001
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | VII, 119 S. Ill. |
Internformat
MARC
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| 100 | 1 | |a Zink, Nicola |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Gene targeting in embryonic stem cells |b = Gene-Targeting in embryonalen Stammzellen |c von Nicola Zink |
| 246 | 1 | 1 | |a Gene-Targeting in embryonalen Stammzellen |
| 264 | 1 | |c 2001 | |
| 300 | |a VII, 119 S. |b Ill. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Univ., Diss., 2001 | ||
| 650 | 7 | |a Mice |2 cabt | |
| 650 | 7 | |a Gene Transfer |2 cabt | |
| 650 | 7 | |a Molecular Genetics |2 cabt | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009592028&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009592028 | |
Datensatz im Suchindex
| _version_ | 1807322675706593280 |
|---|---|
| adam_text |
CONTENTS
CONTENTS
.
I-III
ABBREVIATIONS.
IV-VII
1.
INTRODUCTION
.
1
2.
TRANSGENESIS
.
3
2.1
VIRAL
VECTORS
.
3
2.2
PRONUCLEAR
DNA
MICROINJECTION
.
4
2.3
SPERM-MEDIATED
GENE
TRANSFER
.
5
2.4
GENE
TARGETING
IN
PLURIPOTENT
STEM
CELLS
.
6
2.4.1
EMBRYONIC
CARCINOMA
(EC)
CELLS
.
6
2.4.2
EMBRYONIC
STEM
(ES)
CELLS
.
7
2.4.3
EMBRYONIC
GERM
(EG)
CELLS
.
10
2.5
NUCLEAR
TRANSFER
USING
TRANSFECTED
CELLS
.
11
3.
EMBRYONIC
STEM
(ES)
CELLS
.
13
3.1
CHARACTERISTICS
OF
ES
CELLS
.
13
3.2
ORIGIN
OF
ES
CELLS
.
14
3.3
KARYOTYPE
.
15
3.4
MOUSE
STRAINS
.
17
3.5
PLURIPOTENCY
.
19
3.6
CONTROL
OF
ES
CELL
DIFFERENTIATION
.
20
3.6.1
IN
VITRO
DIFFERENTIATION
OF
ES
CELLS
.
20
3.6.2
FEEDER
CELLS
.
23
3.6.3
MEDIUM
CONDITIONED
BY
DIFFERENT
CELL
LINES
.
25
3.6.4
LEUKEMIA
INHIBITORY
FACTOR
(LIF)
.
26
3.7
ES
CELL
CULTURE
.
28
3.7.1
GENERAL
CONDITIONS
.
28
3.7.2
EQUIPMENT,
REAGENTS,
AND
CELLS
.
29
3.7.3
SUB-CULTURING
ES
CELLS
.
31
3.7.4
FREEZING
ES
CELLS
.
32
3.7.5
THAWING
ES
CELLS
.
33
3.8
HUMAN
ES
CELLS
.
33
4.
TARGETING
VECTORS
.
36
4.1
REPLACEMENT
VECTOR
.
38
4.2
INSERTION
VECTOR
.
39
II
CONTENTS
4.3
EFFICIENCY
OF
GENE
TARGETING
.
40
4.4
ENRICHMENT
FOR
GENE
TARGETING
EVENTS
.
43
4.5
CONDITIONAL
KNOCK-OUTS
.
44
4.6
INTRODUCTION
OF
SUBTLE
MUTATIONS
.47
5.
TRANSFECTION
.49
5.1
CALCIUM
PHOSPHATE
CO-PRECIPITATION
.
49
5.2
MICROINJECTION
.
50
5.3
ELECTROPORATION
.
50
5.4
PRODUCTION
OF
HOMOZYGOUS
MUTANT
ES
CELLS
.
55
6.
SCREENING
STRATEGIES
.
58
6.1
DNA
ISOLATION
FROM
ES
CELL
COLONIES
.
58
6.2
POLYMERASE
CHAIN
REACTION
(PCR)
.
59
6.3
SOUTHERN
BLOT
ANALYSIS
.
61
7.
PRODUCTION
OF
CHIMERAS
.
64
7.1
GENERATING
CHIMERAS
.
64
7.2
METHODS
TO
GENERATE
CHIMERAS
.
65
7.2.1
BLASTOCYST
INJECTION
.
65
7.2.2
MORULA
INJECTION
.
65
7.2.3
MORULA
AGGREGATION
.66
7.2.4
AGGREGATION
WITH
TETRAPIOID
CELLS
.
67
7.3
LABORATORY
MICE:
EMBRYO
RECOVERY
AND
TRANSFER
.
68
7.3.1
DONOR
MICE:
EMBRYO
RECOVERY
.69
7.3.2
HOSTS
FOR
EMBRYO
TRANSFER:
PSEUDOPREGNANT
RECIPIENTS
.
70
7.3.3
MALE
MICE
.
71
7.4.
EMBRYO
RECOVERY
.
71
7.5.
ES
CELLS
.
73
7.6
BLASTOCYST
INJECTION
.
75
7.6.1
MICROINJECTION
APPARATUS
.
75
7.6.2
PIPETTES
.
76
7.6.2.1
HOLDING
PIPETTES:
.
77
7.6.2.2
INJECTION
PIPETTES:
.
77
7.6.2.3
EMBRYO
TRANSFER
PIPETTES:
.
78
7.7
EMBRYO
TRANSFER
.
80
7.8
ES
CELL
CHIMERAS
.
82
7.8.1
DETECTION
AND
QUANTIFICATION
OF
CHIMERISM
.83
ILL
CONTENTS
7.8.1.1
COAT
COLOR
.
83
7.8.1.2
GPI
.
84
7.8.2
PHENOTYPIC
EFFECTS:
SEX
RATIO
IN
CHIMERAS
.
85
7.8.3
CHIMERA
ANALYSIS
.
85
8.
KNOCK-OUT
MICE
.
87
8.1
TEST
BREEDING
.
87
8.2 CREATING
KNOCK-OUT
MICE
.
87
8.3
ANALYSIS
OF
KNOCK-OUT
MICE
.
88
8.4
MOUSE
MODELS
OF
HUMAN
DISEASES
.
89
8.5
LIMITATIONS
OF
KNOCK-OUT
MICE
.
91
9.
SUMMARY
.
92
10.
REFERENCES
.
95
ACKNOWLEDGEMENT
.
121 |
| any_adam_object | 1 |
| author | Zink, Nicola |
| author_facet | Zink, Nicola |
| author_role | aut |
| author_sort | Zink, Nicola |
| author_variant | n z nz |
| building | Verbundindex |
| bvnumber | BV014009999 |
| ctrlnum | (OCoLC)49725681 (DE-599)BVBBV014009999 |
| discipline | Medizin |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV014009999 |
| illustrated | Illustrated |
| indexdate | 2024-08-14T01:00:16Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009592028 |
| oclc_num | 49725681 |
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| owner_facet | DE-19 DE-BY-UBM DE-355 DE-BY-UBR DE-12 DE-188 |
| physical | VII, 119 S. Ill. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| record_format | marc |
| spelling | Zink, Nicola Verfasser aut Gene targeting in embryonic stem cells = Gene-Targeting in embryonalen Stammzellen von Nicola Zink Gene-Targeting in embryonalen Stammzellen 2001 VII, 119 S. Ill. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2001 Mice cabt Gene Transfer cabt Molecular Genetics cabt (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009592028&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zink, Nicola Gene targeting in embryonic stem cells = Gene-Targeting in embryonalen Stammzellen Mice cabt Gene Transfer cabt Molecular Genetics cabt |
| subject_GND | (DE-588)4113937-9 |
| title | Gene targeting in embryonic stem cells = Gene-Targeting in embryonalen Stammzellen |
| title_alt | Gene-Targeting in embryonalen Stammzellen |
| title_auth | Gene targeting in embryonic stem cells = Gene-Targeting in embryonalen Stammzellen |
| title_exact_search | Gene targeting in embryonic stem cells = Gene-Targeting in embryonalen Stammzellen |
| title_full | Gene targeting in embryonic stem cells = Gene-Targeting in embryonalen Stammzellen von Nicola Zink |
| title_fullStr | Gene targeting in embryonic stem cells = Gene-Targeting in embryonalen Stammzellen von Nicola Zink |
| title_full_unstemmed | Gene targeting in embryonic stem cells = Gene-Targeting in embryonalen Stammzellen von Nicola Zink |
| title_short | Gene targeting in embryonic stem cells |
| title_sort | gene targeting in embryonic stem cells gene targeting in embryonalen stammzellen |
| title_sub | = Gene-Targeting in embryonalen Stammzellen |
| topic | Mice cabt Gene Transfer cabt Molecular Genetics cabt |
| topic_facet | Mice Gene Transfer Molecular Genetics Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009592028&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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