Proteomics in practice: a laboratory manual of proteome analysis
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Weinheim
Wiley-VCH
2002
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXIV, 316 S. Ill., graph. Darst. |
| ISBN: | 3527303545 |
Internformat
MARC
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| 100 | 1 | |a Westermeier, Reiner |d 1951- |e Verfasser |0 (DE-588)135840627 |4 aut | |
| 245 | 1 | 0 | |a Proteomics in practice |b a laboratory manual of proteome analysis |c Reiner Westermeier ; Tom Naven |
| 264 | 1 | |a Weinheim |b Wiley-VCH |c 2002 | |
| 300 | |a XXIV, 316 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a Biologie moléculaire | |
| 650 | 7 | |a Eiwitten |2 gtt | |
| 650 | 7 | |a Elektroforese |2 gtt | |
| 650 | 7 | |a Massaspectrometrie |2 gtt | |
| 650 | 7 | |a Proteolyse |2 gtt | |
| 650 | 7 | |a Proteínas |2 larpcal | |
| 650 | 4 | |a Protéines - Analyse | |
| 650 | 4 | |a Proteome |x analysis |v Laboratory Manuals | |
| 650 | 4 | |a Proteomics |v Handbooks, manuals, etc | |
| 650 | 4 | |a Proteomics |x methods |v Laboratory Manuals | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009583435 | |
Datensatz im Suchindex
| _version_ | 1826205054462328832 |
|---|---|
| adam_text |
V
CONTENTS
PREFACE
IX
FOREWORD
XI
ABBREVIATIONS,
SYMBOLS,
UNITS
XIII
GLOSSARY
OF
TERMS
XVII
PARTI:
PROTEOMICS
TECHNOLOGY
1
1
1.1
1.2
1.3
1.4
1.5
1.6
1.7
INTRODUCTION
1
APPLICATIONS
OF
PROTEOMICS
6
SEPARATION
OF
THE
PROTEIN
MIXTURES
6
DETECTION
7
IMAGE
ANALYSIS
7
IDENTIFICATION
OF
PROTEINS
8
CHARACTERIZATION
OF
PROTEINS
9
FUNCTIONAL
PROTEOMICS
9
2
2.1
2.1.1
2.1.2
2.1.3
2.1.4
2.1.5
2.1.6
2.2
2.2.1
2.2.2
2.3
2.3.1
2.3.2
EXPRESSION
PROTEOMICS
11
TWO-DIMENSIONAL
ELECTROPHORESIS
11
EVOLUTION
OF
THE
2-D
METHODOLOGY
12
SAMPLE
PREPARATION
15
FIRST
DIMENSION:
ISOELECTRIC
FOCUSING
27
SECOND
DIMENSION:
SDS-PAGE
57
DETECTION
OF
PROTEIN
SPOTS
79
IMAGE
ANALYSIS
87
SPOT
HANDLING
97
SPOT
CUTTING
98
PROTEIN
CLEAVAGE
100
MASS
SPECTROMETRY
108
IONISATION
112
ION
SEPARATION
118
VII
CONTENTS
2.3.3
2.4
2.4.1
2.4.2
TANDEM
MASS
SPECTROMETRY
(MS/MS)
125
PROTEIN
IDENTIFICATION
BY
DATABASE
SEARCHING
135
PEPTIDE
MASS
FINGERPRINT
136
PEPTIDE
MASS
FINGERPRINTING
COMBINED
WITH
COMPOSITION
INFORMATION
138
2.4.3
PEPTIDE
MASS
FINGERPRINT
COMBINED
WITH
PARTIAL
SEQUENCE
INFORMATION
139
2.4.4
2.4.5
2.5
2.5.1
2.5.2
2.5.3
PRODUCT
ION
MS/MS
SEQUENCE DATA
143
DE
NOVO
SEQUENCING
145
METHODS
OF
PROTEOME
ANALYSIS
154
2D-MS
154
LC-MS/MS
157
QUANTITATIVE
PROTEOMICS
159
PART
II
COURSE
MANUAL
161
EQUIPMENT,
CONSUMABLES,
REAGENTS
163
STEP
1:
SAMPLE
PREPARATION
169
1
2
3
4
5
6
7
8
STOCK
SOLUTIONS
170
EXAMPLES
172
MICRODIALYSIS
175
PRECIPITATION
177
BASIC
PROTEINS
181
VERY
HYDROPHOBIC
PROTEINS
182
QUANTIFICATION
184
SDS
SAMPLES
FOR
BMW
PROTEINS
SEPARATION
186
STEP
2:
ISOELECTRIC
FOCUSING
187
1
2
3
4
5
RESWELLING
TRAY
188
REHYDRATION
LOADING
AND
IEF
IN
IPGPHOR
STRIP
HOLDERS
190
IEF
IN
THE
CUP
LOADING
STRIP
HOLDER
(REHYDRATION
LOADED
STRIPS)
192
CUP
LOADING
IEF
194
STAINING
OF
I
PG
STRIPS
196
STEP
3:
SDS
POLYACRYLAMIDE
CEL
ELECTROPHORESIS
199
1
1.1
1.2
1.3
1.3.2
1.3.3
1.4
2
3
CASTING
OF
SDS
POLYACRYLAMIDE
GELS
199
STOCK
SOLUTIONS
199
CASSETTES
FOR
LABORATORY
MADE
GELS
201
MULTIPLE
GEL
CASTER
(UP
TO
14
GELS)
203
HOMOGENEOUS
GELS
204
GRADIENT
GELS
207
GEL
CASTER
FOR
UP
TO
SIX
GELS
212
INSERTING
READY-MADE
GELS
INTO
CASSETTES
219
PREPARATION
OF
THE
SDS
ELECTROPHORESIS
EQUIPMENT
222
CONTENTS
IVII
3.1
STOCK
SOLUTIONS
FOR
THE
RUNNING
BUFFERS
222
3.2
SETTING
UP
THE
INTEGRATED
HIGH-THROUGHPUT
INSTRUMENT
223
3.3
SETTING
UP
THE
SIX
GEL
INSTRUMENT
223
4
EQUILIBRATION
OF
THE
IPG
STRIPS
AND
TRANSFER
TO
THE
SDS
GELS
224
4.1
EQUILIBRATION
224
4.2
APPLICATION
OF
THE
IPG
STRIPS
ONTO
THE
SDS
GELS
224
4.3
APPLICATION
OF
MOLECULAR
WEIGHT
MARKER
PROTEINS
AND
1-D
SAMPLES
226
4.4
SEAL
THE
IPG
STRIP
AND
THE
SDS
GEL
226
5
THE
SDS
ELECTROPHORESIS
RUN
227
5.1
THE
INTEGRATED
HIGH-THROUGHPUT
INSTRUMENT
227
5.2
THE
SIX-GEL
INSTRUMENT
WITH
STANDARD
POWER
SUPPLY
229
STEP
4:
STAINING
OF
THE
GELS
233
1
COLLOIDAL
COOMASSIE
BRILLIANT
BLUE
STAINING
233
1.1
SOLUTIONS
233
1.2
STAINING
234
2
HOT
COOMASSIE
BRILLIANT
BLUE
STAINING
234
3
SILVER
STAINING
235
4
FLUORESCENCE
STAINING
238
5
PRESERVING
AND
DRYING
OF
GELS
238
STEP
5:
SCANNING
OF
GELS
AND
IMAGE
ANALYSIS
239
1
SCANNING
239
1.1
GELS
STAINED
WITH
VISIBLE
DYES
239
1.2
SCANNING
FLUORESCENT
DYES
241
2
SPOT
DETECTION
AND
BACKGROUND
PARAMETERS
241
2.1
AUTOMATED SPOT
DETECTION
241
2.2
SPOT
FILTERING
242
2.3
SPOT
EDITING
242
T
2.4
BACKGROUND
CORRECTION
243
3
EVALUATION
OF
2-D
PATTERNS
243
3.1
REFERENCE
GEL
243
3.2
AUTOMATIC
GEL
MATCHING
244
3.3
NORMALISATION
245
3.4
PL
AND
M
R
CALIBRATION
245
3.5
DIFFERENCE
MAPS
246
3.6
AVERAGED
GELS
247
3.7
QUANTIFICATION
247
3.8
CREATING
REPORTS
247
3.9
SPOT
PICKING
247
STEP
6:
FLUORESCENCE
DIFFERENCE
GEL
ELECTROPHORESIS
249
1
PREPARING
A
CELL
LYSATE
COMPATIBLE
WITH
CYDYE
LABELLING
250
2
RECONSTITUTING
THE
STOCK
CYDYE
IN
DIMETHYLFORMAMIDE
(DMF)
251
3
PREPARING
CYDYE
SOLUTION
USED
TO
LABEL
PROTEINS
252
VIII
CONTENTS
STEP
7:
SPOT
EXCISION
255
STEP
8:
SAMPLE
DESTAINING
259
STEP
9:
IN-GEL
DIGESTION
261
STEP
10:
MICROSCALE
DESALTING
AND
CONCENTRATION
OF
SAMPLE
263
STEP
11:
CHEMICAL
DERIVATISATION
OF
THE
PEPTIDE
DIGEST
265
STEP
12:
MS
ANALYSIS
269
STEP
13:
CALIBRATION
OF
THE
MALDI-TOF
MS
273
STEP
14:
PREPARING
FOR
A
DATABASE
SEARCH
277
STEP
15:
PM
F
DATABASE
SEARCH
UNSUCCESSFUL
281
A
TROUBLE
SHOOTING
283
1
TWO-DIMENSIONAL
ELECTROPHORESIS
283
1.1
ISOELECTRIC
FOCUSING
IN
IPG
STRIPS
283
1.2
SDS
PAGE
285
1.3
STAINING
286
1.4
DIGE
FLUORESCENCE
LABELLING
287
1.5
RESULTS
IN
2-D
ELECTROPHORESIS
288
2
MASS
SPECTROMETRY
291
REFERENCES
295
INDEX
311 |
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| dewey-ones | 572 - Biochemistry |
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| id | DE-604.BV013999531 |
| illustrated | Illustrated |
| indexdate | 2025-03-10T11:07:36Z |
| institution | BVB |
| isbn | 3527303545 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009583435 |
| oclc_num | 55689485 |
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| physical | XXIV, 316 S. Ill., graph. Darst. |
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| publisher | Wiley-VCH |
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| spelling | Westermeier, Reiner 1951- Verfasser (DE-588)135840627 aut Proteomics in practice a laboratory manual of proteome analysis Reiner Westermeier ; Tom Naven Weinheim Wiley-VCH 2002 XXIV, 316 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biologie moléculaire Eiwitten gtt Elektroforese gtt Massaspectrometrie gtt Proteolyse gtt Proteínas larpcal Protéines - Analyse Proteome analysis Laboratory Manuals Proteomics Handbooks, manuals, etc Proteomics methods Laboratory Manuals Praktikum (DE-588)4127380-1 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 s DE-604 Praktikum (DE-588)4127380-1 s Naven, Tom Verfasser aut DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009583435&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Westermeier, Reiner 1951- Naven, Tom Proteomics in practice a laboratory manual of proteome analysis Biologie moléculaire Eiwitten gtt Elektroforese gtt Massaspectrometrie gtt Proteolyse gtt Proteínas larpcal Protéines - Analyse Proteome analysis Laboratory Manuals Proteomics Handbooks, manuals, etc Proteomics methods Laboratory Manuals Praktikum (DE-588)4127380-1 gnd Proteomanalyse (DE-588)4596545-6 gnd |
| subject_GND | (DE-588)4127380-1 (DE-588)4596545-6 |
| title | Proteomics in practice a laboratory manual of proteome analysis |
| title_auth | Proteomics in practice a laboratory manual of proteome analysis |
| title_exact_search | Proteomics in practice a laboratory manual of proteome analysis |
| title_full | Proteomics in practice a laboratory manual of proteome analysis Reiner Westermeier ; Tom Naven |
| title_fullStr | Proteomics in practice a laboratory manual of proteome analysis Reiner Westermeier ; Tom Naven |
| title_full_unstemmed | Proteomics in practice a laboratory manual of proteome analysis Reiner Westermeier ; Tom Naven |
| title_short | Proteomics in practice |
| title_sort | proteomics in practice a laboratory manual of proteome analysis |
| title_sub | a laboratory manual of proteome analysis |
| topic | Biologie moléculaire Eiwitten gtt Elektroforese gtt Massaspectrometrie gtt Proteolyse gtt Proteínas larpcal Protéines - Analyse Proteome analysis Laboratory Manuals Proteomics Handbooks, manuals, etc Proteomics methods Laboratory Manuals Praktikum (DE-588)4127380-1 gnd Proteomanalyse (DE-588)4596545-6 gnd |
| topic_facet | Biologie moléculaire Eiwitten Elektroforese Massaspectrometrie Proteolyse Proteínas Protéines - Analyse Proteome analysis Laboratory Manuals Proteomics Handbooks, manuals, etc Proteomics methods Laboratory Manuals Praktikum Proteomanalyse |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009583435&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT westermeierreiner proteomicsinpracticealaboratorymanualofproteomeanalysis AT naventom proteomicsinpracticealaboratorymanualofproteomeanalysis |