Towards the development of novel cancer therapies: selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Karlsruhe
Forschungszentrum Karlsruhe
2001
|
| Ausgabe: | Als Ms. gedr. |
| Schriftenreihe: | Wissenschaftliche Berichte / Forschungszentrum Karlsruhe Technik und Umwelt
6654 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: Karlsruhe, Univ., Diss., 2001 |
| Beschreibung: | XVII, 161 S. Ill., graph. Darst. |
Internformat
MARC
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| 016 | 7 | |a 963029959 |2 DE-101 | |
| 035 | |a (OCoLC)216578945 | ||
| 035 | |a (DE-599)BVBBV013990839 | ||
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| 041 | 0 | |a eng | |
| 049 | |a DE-12 |a DE-91 | ||
| 088 | |a FZKA 6654 | ||
| 100 | 1 | |a Kirkin, Vladimir |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Towards the development of novel cancer therapies |b selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis |c Vladimir Kirkin |
| 250 | |a Als Ms. gedr. | ||
| 264 | 1 | |a Karlsruhe |b Forschungszentrum Karlsruhe |c 2001 | |
| 300 | |a XVII, 161 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Wissenschaftliche Berichte / Forschungszentrum Karlsruhe Technik und Umwelt |v 6654 | |
| 500 | |a Zugl.: Karlsruhe, Univ., Diss., 2001 | ||
| 650 | 0 | 7 | |a In-vitro-Kultur |0 (DE-588)4332883-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Inhibition |0 (DE-588)4273127-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Angiogenese |0 (DE-588)4142449-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Tumorhemmung |0 (DE-588)4292868-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Vascular endothelial Growth Factor |0 (DE-588)4319404-7 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Vascular endothelial Growth Factor |0 (DE-588)4319404-7 |D s |
| 689 | 0 | 1 | |a Inhibition |0 (DE-588)4273127-6 |D s |
| 689 | 0 | 2 | |a Tumorhemmung |0 (DE-588)4292868-0 |D s |
| 689 | 0 | 3 | |a Angiogenese |0 (DE-588)4142449-9 |D s |
| 689 | 0 | 4 | |a In-vitro-Kultur |0 (DE-588)4332883-0 |D s |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009576198 | |
Datensatz im Suchindex
| _version_ | 1807772525683277824 |
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| adam_text |
TABLE
OF
CONTENTS
ZUSAMMENFASUNG
.
I
ABSTRACT
.
II
ACKNOWLEDGEMENTS
.
III
TABLE
OF
CONTENTS
.
V
LIST
OF
FIGURES
.
XI
LIST
OF
TABLES
.
XIII
ABBREVIATIONS
.
XIV
CHAPTER
1:
INTRODUCTION
.
1
1.1
CANCER
.
2
1.1.1
TUMOUR
GENERATION
.
2
1.1.2
APOPTOSIS
AND
TUMORIGENESIS
.
2
1.1.3
TUMOUR
PROGRESSION
AND
METASTASIS
.
3
1.1.4
TUMOUR-HOST
INTERACTIONS
.
4
1.1.5
TUMOUR
ANGIOGENESIS
AND
LYMPHANGIOGENESIS
.
6
1.1.6
APPROACHES
TO
ANTI-CANCER
THERAPY
.
6
1.2
ANGIOGENESIS
.
7
1.2.1
VASCULOGENESIS
VS.
ANGIOGENESIS
.
7
1.2.2
MOLECULAR
MECHANISMS
OF
ANGIOGENESIS
REGULATION
.
9
1.2.2.1
POSITIVE
REGULATORS
OF
ANGIOGENESIS
.
9
A)
VASCULAR
ENDOTHELIAL
GROWTH
FACTOR
(VEGF)
FAMILY
AND
VEGFR
RECEPTORS
.
9
B)
FIBROBLAST
GROWTH
FACTOR
(FGF)
FAMILY
AND
RECEPTORS
.
14
C)
INDIRECTLY
ACTING
ANGIOGENIC
FACTORS
.
16
D)
TIE
RECEPTORS
AND
ANGIOPOIETINS
.
16
E)
INTEGRIN
RECEPTOR
FAMILY
.
18
1.2.2.2
NEGATIVE
REGULATORS
OF
ANGIOGENESIS
.
18
1.2.3
TUMOUR
ANGIOGENESIS
.
20
1.3
LYMPHANGIOGENESIS
.
22
1.3.1
LYMPHATIC
SYSTEM
AND
ITS
FUNCTION
.
22
1.3.2
LYMPHANGIOGENESIS
IN
EMBRYO
AND
ADULT
.
23
1.3.3
SIGNALLING
LYMPHANGIOGENESIS
.
23
1.3.3.1
ROLE
OF
VEGFR-3
IN
LYMPHANGIOGENESIS
.
24
1.3.3.2
VEGF-C
AND
VEGF-D
IN
LYMPHANGIOGENESIS
.
25
1.3.4
TUMOUR-INDUCED
LYMPHANGIOGENESIS
.
27
1.4
INHIBITION
OF
ANGIOGENESIS:
A
NOVEL
ANTICANCER
THERAPY
.
30
1.4.1
ROLE
OF
ANTI-ANGIOGENIC
STRATEGY
IN
FIGHTING
CANCER
.
30
1.4.2
INHIBITION
OF
ANGIOGENIC
LIGAND
SIGNALLING
.
31
1.4.3
TARGETING
ENDOTHELIAL
CELL-ECM
INTERACTIONS
.
33
1.4.4
DIRECT
INHIBITION
OF
ENDOTHELIAL
CELL
PROLIFERATION
.
34
1.5
AIMS
OF
THE
STUDY
.
35
CHAPTER
2:
MATERIALS
AND
METHODS
.
37
2.1
MATERIALS
.
38
2.1.1
CHEMICALS
.
38
2.1.2
OLIGONUCLEOTIDES
.
38
2.1.3
ANTIBODIES
AND
CYTOKINES
.
38
2.1.4
BACTERIA
.
39
2.1.5
CELL
LINES
AND
MEDIA
.
39
2.2
GENERAL
METHODS
.
41
2.2.1
PHENOL/CHLOROFORM
EXTRACTION
OF
NUCLEIC
ACIDS
.
41
2.2.2
ETHANOL
(OR
2-PROPANOL)
PRECIPITATION
OF
NUCLEIC
ACIDS
.
41
2.2.3
DETERMINATION
OF
NUCLEIC
ACID
CONCENTRATION
.
41
2.2.4
TOTAL
RNA
ISOLATION
FROM
CELLS
OR
TISSUE
.
41
2.2.5
REVERSE
TRANSCRIPTION
POLYMERASE
CHAIN
REACTION
(RT-PCR)
.
42
2.2.5.1
FIRST
STRAND
CDNA
SYNTHESIS
.
42
2.2.5.2
AMPLIFICATION
OF
FIRST
STRAND
CDNA
BY
PCR
.
42
2.2.6
POLYMERASE
CHAIN
REACTION
(PCR)
DNA
AMPLIFICATION
.
43
2.2.7
RECOMBINANT
PCR
.
43
2.2.8
RESTRICTION
ENDONUCLEASE
DIGESTION
OF
DNA
.
45
2.2.9
DNA
LIGATION
.
45
2.2.10
SUB-CLONING
.
45
2.2.11
SIZE
SEPARATION
OF
NUCLEIC
ACID
BY
AGAROSE
GEL
ELECTROPHORESIS
.
45
2.2.12
ISOLATION/PURIFICATION
OF
DNA
FROM
AGAROSE
GELS
.
46
-
VI
-
2.2.13
E.
COLI
STRAIN
MAINTENANCE
.
46
2.2.14
GROWTH
OF
E.
COLI
CULTURE
IN
LIQUID
MEDIUM
.
46
2.2.15
PREPARATION
OF
COMPETENT
BACTERIA
(E.
COLI)
.
46
2.2.15.1
CHEMICALLY
COMPETENT
E.
COLI
(CALCIUM
CHLORIDE
METHOD)
.
46
2.2.15.2
ELECTROCOMPETENT
E.
COLI
.
47
2.2.16
TRANSFORMATION
OF
E.
COLI
.
41
2.2.16.1
CHEMICALLY
.
47
2.2.16.2
ELECTROPORATION
.
48
2.2.17
PLASMID
DNA
PREPARATION
.
48
2.2.17.1
SMALL
SCALE
METHOD
-
1
.
48
2.2.17.2
SMALL
SCALE
METHOD
-
2
.
48
2.2.17.3
LARGE
SCALE
PLASMID
PREPARATION
.
49
2.2.18
SEQUENCING
OF
DOUBLE-STRANDED
TEMPLATE
DNA
.
49
2.2.18.1
AUTOMATED
(FLUORESCENCE)
DNA
SEQUENCING
METHOD
.
49
2.2.18.2
MANUAL
(RADIOACTIVE)
DNA
SEQUENCING
METHOD
.
50
2.2.19
CELL
CULTURE
.
50
2.2.20
STABLE
AND
TRANSIENT
TRANSFECTION
OF
CELLS
.
51
2.2.20.1
CALCIUM
PHOSPHATE
METHOD
.
51
2.2.20.2
LIPOFECTION
.
52
2.2.21
PURIFICATION
OF
RECOMBINANT
HIS-TAGGED
PROTEINS
.
52
2.2.22
DETERMINATION
OF
PROTEIN
CONCENTRATION
.
53
2.2.23
CELL
LYSATE
PREPARATION
.
53
2.2.24
SDS-POLYACRYLAMIDE
GEL
ELECTROPHORESIS
(SDS-PAGE)
.
54
2.2.25
STAINING
THE
SDS-PAGE
GELS
.
54
2.2.25.1
COOMASSIE
STAINING
.
54
2.2.25.2
SILVER
STAINING
.
54
2.2.26
WESTERN
BLOTTING
.
54
2.2.27
STRIPPING
WESTERN
BLOT
MEMBRANE
.
55
2.3
ANALYTICAL
METHODS
.
55
2.3.1
IN
VITRO
TYROSINE
KINASE
ASSAY
.
55
2.3.2
CELLULAR
PHOSPHORYLATION
ASSAY
.
56
2.3.3
PROLIFERATION
ASSAY:
(3H)-THYMIDINE
INCORPORATION
.
57
2.3.4
APOPTOSIS
DETECTION
ASSAY
.
58
-
VII
-
2.3.5
CELL
CYCLE
ANALYSIS
.
58
2.3.6
MATRTIGEL-BASED
IN
VIVO
ANGIOGENESIS
ASSAY
.
59
2.3.7
INDUCTION
OF
LYMPHANGIOMAS
IN
VIVO
.
59
2.3.8
TISSUE
EMBEDDING
AND
PREPARATION
OF
SECTIONS
.
59
2.3.8.1
PARAFFIN
SECTIONS
.
59
2.3.8.2
FROZEN
SECTIONS
.
60
2.3.9
TISSUE
SECTION
STAINING
.
60
2.3.9.1
STAINING
WITH
HEMOTOXYLIN
AND
EOSIN
(H&E
STAINING)
.
60
2.3.9.2
MASSON
'
S
TRICHROME
STAINING
.
60
2.3.9.3
IMMMUNOHISTOCHEMISTRY
(IHC)
.
61
2.3.10
ENDOTHELIAL
SPROUTING:
IN
VITRO
ANGIOGENESIS
ASSAY
.
62
2.3.11
IN
VIVO
TUMOUR
GROWTH
INHIBITION
EXPERIMENTS
.
63
2.3.11.1
INHIBITION
OF
TUMOUR
GROWTH
BY
INDOLINONES
.
63
2.3.11.2
INHIBITION
OF
TUMOUR
GROWTH
BY
HYPERFORIN
.
63
CHAPTER
3:
RESULTS
.
64
PART
I:
CREATION
OF
A
CELLULAR
SYSTEM
FOR
ANALYSING
ANTI-TUMOUR
SUBSTANCES
WITH
ANTI-ANGIOGENIC
AND/OR
ANTI-LYMPHANGIOGENIC
PROPERTIES
.
65
3.1
CLONING,
EXPRESSION
AND
PURIFICATION
OF
RECOMBINANT
RAT
VEGFS
.
66
3.1.1
CLONING
RAT
VEGFS
.
66
3.1.1.1
CLONING
RAT
VEGF-C,
VEGF-D
AND
VEGF
.
67
3.1.1.2
CLONING
RAT
ANAC/VEGF-C
AND
ANAC/VEGF-C(CYSL52SER).
68
3.1.1.3
CLONING
RAT
ANAC/VEGF-D
AND
ANAC/VEGF-D(CYSL41SER).
69
3.1.2
EXPRESSION
AND
PURIFICATION
OF
RECOMBINANT
VEGFS
.
72
3.2
CHARACTERISATION
OF
RECOMBINANT
RAT
VEGF
PROTEINS
.
72
3.2.1
ACTIVATION
OF
VEGF
RECEPTORS
.
72
3.2.1.1
VEGF,
ANAC/VEGF-C
AND
ANAC/VEGF-D
CONDITIONED
MEDIUM
.
74
3.2.1.2
ANAC/VEGF-C(CYSL52SER)
AND
ANAC/VEGF-D(CYSL41SER)
CONDITIONED
MEDIUM
.
74
3.2.1.3
PURIFIED
RECOMBINANT
RAT
VEGF
PROTEINS
.
76
3.2.2
STIMULATION
OF
VASCULAR
ENDOTHELIAL
CELL
PROLIFERATION
.
78
3.3
ESTABLISHMENT
OF
RAT
LYMPHATIC
ENDOTHELIUM
('LE')
IN
CULTURE
.
78
-
VIII
-
3.3.1
USE
OF
RECOMBINANT
ANAC/VEGF-C
IN
VIVO
.
80
3.3.2
USE
OF
ADJUVANT-INDUCED
LYMPHANGIOMAS.
81
PART
II:
SELECTION
AND
CHARACTERISATION
OF
ANTI-TUMOUR
SUBSTANCES
WITH
ANTI
ANGIOGENIC
AND/OR
ANTI-LYMPHANGIOGENIC
PROPERTIES
.
84
3.4
SELECTION
OF
INDOLINONES
INHIBITING
ACTIVATION
OF
TUMOUR-RELATED
RTKS
.
85
3.4.1
PRIMARY
SCREENING
OF
INDOLINONES
FOR
THEIR
INHIBITORY
ACTIVITY
.
85
3.4.2
SCREENING
OF
INDOLINONES
FOR
INHIBITION
OF
CELLULAR
VEGFR
ACTIVITY.
.
87
3.4.2.1
CELLULAR
VEGFR-3
PHOSPHORYLATION
ASSAY
.
87
A)
INHIBITION
OF
VEGF-C-MEDIATED
VEGFR-3
ACTIVATION
.
87
B)
INHIBITION
OF
VEGF-D-MEDIATED
VEGFR-3
ACTIVATION
.
94
3.4.2.2
CELLULAR
VEGFR-2
PHOSPHORYLATION
ASSAY
.
94
3.4.2.3
CELLULAR
VEGFR
1
PHOSPHORYLATION
ASSAY
.
99
3.5
CHARACTERISATION
OF
THE
EFFECT
OF
AE87,
AE106,
MAZ51
AND
MAZ51-2
ON
ANGIOGENESIS,
LYMPHANGIOGENESIS
AND
TUMOUR
GROWTH
.
99
3.5.1
ANTI-ANGIOGENIC
PROPERTIES
OF
AE87,
AE
106,
MAZ5
1
AND
MAZ5
1-2.
99
3.5.1.1
INHIBITION
OF
ENDOTHELIAL
CELL
PROLIFERATION
.
99
3.5.1.2
DIFFERENTIAL
INHIBITION
OF
VEGF
AND
FGF-MEDIATED
VASCULAR
ENDOTHELIAL
CELL
PROLIFERATION
.
100
3.5.1.3
INDUCTION
OF
APOPTOSIS
IN
ENDOTHELIAL
CELLS
.
101
3.5.1.4
INHIBITION
OF
ENDOTHELIAL
CELL
SPROUTING
.
105
3.5.2
ANTI-TUMOUR
PROPERTIES
OF
AE87,
AE
106,
MAZ5
1
.
105
3.5.2.1
INHIBITION
OF
TUMOUR
CELL
PROLIFERATION
IN
VITRO
.
107
3.5.2.2
INDUCTION
OF
APOPTOSIS
IN
TUMOUR
CELLS
IN
VITRO
.
110
3.5.2.3
EFFECT
OF
INDOLINONES
ON
IAS
RAT
PANCREATIC
CARCINOMA
TUMOUR
GROWTH
IN
VIVO
.
110
3.5.2.4
EFFECT
OF
MAZ51
ON
MT450
RAT
MAMMARY
CARCINOMA
TUMOUR
GROWTH
IN
VIVO.
113
3.5.2.5
EFFECT
OF
MAZ51
ON
THE
GROWTH
OF
ESTABLISHED
MT450
RAT
MAMMARY
CARCINOMA
TUMOURS
IN
VIVO
.
115
3.6
CHARACTERISATION
OF
ANTI-TUMOUR
PROPERTIES
OF
PLANT-DERIVED
HYPERFORIN
.
117
3.6.1
ANTI-PROLIFERATIVE
ACTION
OF
HYPERFORIN
ON
TUMOUR
CELLS
AND
VASCULAR
ENDOTHELIAL
CELLS
IN
VITRO
.
117
3.6.2
INDUCTION
OF
APOPTOSIS
BY
HYPERFORIN
IN
VITRO
.
119
-
IX
-
3.6.3
ANTI-TUMOUR
ACTIVITY
OF
HYPERFORIN
IN
VIVO
.
119
3.7
PATENTS
AND
PUBLICATIONS
.
123
CHAPTER
4:
DISCUSSION
.
124
4.1
CREATION
OF
CELLULAR
ASSAYS
FOR
RTK
ACTIVITY
.
125
4.1.1
RAT
VEGF-C
AND
VEGF-D
.
125
4.1.2
ATTEMPTS
TO
ESTABLISH
LE
CULTURES
.
126
4.2
SELECTION
AND
CHARACTERISATION
OF
INDOLINONES
.
129
4.2.1
SCREENING
PROCEDURE
.
129
4.2.2
PROPERTIES
OF
AE87,
AE1
06,
MAZ5
1
AND
MAZ5
1-2
.
130
4.3
HYPERFORIN:
A
NOVEL
ANTI-CANCER
DRUG
.
133
4,4
CONCLUSION
.
134
REFERENCES
.
135
RESUME
.
160
-
X
- |
| any_adam_object | 1 |
| author | Kirkin, Vladimir |
| author_facet | Kirkin, Vladimir |
| author_role | aut |
| author_sort | Kirkin, Vladimir |
| author_variant | v k vk |
| building | Verbundindex |
| bvnumber | BV013990839 |
| ctrlnum | (OCoLC)216578945 (DE-599)BVBBV013990839 |
| edition | Als Ms. gedr. |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV013990839 |
| illustrated | Illustrated |
| indexdate | 2024-08-19T00:10:26Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009576198 |
| oclc_num | 216578945 |
| open_access_boolean | |
| owner | DE-12 DE-91 DE-BY-TUM |
| owner_facet | DE-12 DE-91 DE-BY-TUM |
| physical | XVII, 161 S. Ill., graph. Darst. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Forschungszentrum Karlsruhe |
| record_format | marc |
| series2 | Wissenschaftliche Berichte / Forschungszentrum Karlsruhe Technik und Umwelt |
| spelling | Kirkin, Vladimir Verfasser aut Towards the development of novel cancer therapies selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis Vladimir Kirkin Als Ms. gedr. Karlsruhe Forschungszentrum Karlsruhe 2001 XVII, 161 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Wissenschaftliche Berichte / Forschungszentrum Karlsruhe Technik und Umwelt 6654 Zugl.: Karlsruhe, Univ., Diss., 2001 In-vitro-Kultur (DE-588)4332883-0 gnd rswk-swf Inhibition (DE-588)4273127-6 gnd rswk-swf Angiogenese (DE-588)4142449-9 gnd rswk-swf Tumorhemmung (DE-588)4292868-0 gnd rswk-swf Vascular endothelial Growth Factor (DE-588)4319404-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Vascular endothelial Growth Factor (DE-588)4319404-7 s Inhibition (DE-588)4273127-6 s Tumorhemmung (DE-588)4292868-0 s Angiogenese (DE-588)4142449-9 s In-vitro-Kultur (DE-588)4332883-0 s DE-604 Forschungszentrum Karlsruhe Technik und Umwelt Wissenschaftliche Berichte 6654 (DE-604)BV010181833 6654 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009576198&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kirkin, Vladimir Towards the development of novel cancer therapies selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis In-vitro-Kultur (DE-588)4332883-0 gnd Inhibition (DE-588)4273127-6 gnd Angiogenese (DE-588)4142449-9 gnd Tumorhemmung (DE-588)4292868-0 gnd Vascular endothelial Growth Factor (DE-588)4319404-7 gnd |
| subject_GND | (DE-588)4332883-0 (DE-588)4273127-6 (DE-588)4142449-9 (DE-588)4292868-0 (DE-588)4319404-7 (DE-588)4113937-9 |
| title | Towards the development of novel cancer therapies selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis |
| title_auth | Towards the development of novel cancer therapies selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis |
| title_exact_search | Towards the development of novel cancer therapies selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis |
| title_full | Towards the development of novel cancer therapies selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis Vladimir Kirkin |
| title_fullStr | Towards the development of novel cancer therapies selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis Vladimir Kirkin |
| title_full_unstemmed | Towards the development of novel cancer therapies selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis Vladimir Kirkin |
| title_short | Towards the development of novel cancer therapies |
| title_sort | towards the development of novel cancer therapies selection and characterisation of compounds that inhibit tumour cell proliferation angiogenesis and lymphangiogenesis |
| title_sub | selection and characterisation of compounds that inhibit tumour cell proliferation, angiogenesis and lymphangiogenesis |
| topic | In-vitro-Kultur (DE-588)4332883-0 gnd Inhibition (DE-588)4273127-6 gnd Angiogenese (DE-588)4142449-9 gnd Tumorhemmung (DE-588)4292868-0 gnd Vascular endothelial Growth Factor (DE-588)4319404-7 gnd |
| topic_facet | In-vitro-Kultur Inhibition Angiogenese Tumorhemmung Vascular endothelial Growth Factor Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009576198&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV010181833 |
| work_keys_str_mv | AT kirkinvladimir towardsthedevelopmentofnovelcancertherapiesselectionandcharacterisationofcompoundsthatinhibittumourcellproliferationangiogenesisandlymphangiogenesis |