Epstein-Barr virus protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Schriftenreihe: | Methods in molecular biology
174 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 438 S. graph. Darst. |
| ISBN: | 0896036901 |
Internformat
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| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2001 | |
| 300 | |a XIV, 438 S. |b graph. Darst. | ||
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| 650 | 4 | |a Herpesvirus 4, Human |x isolation & purification |v Laboratory Manuals | |
| 650 | 4 | |a Herpesvirus 4, Human |x physiology |v Laboratory Manuals | |
| 650 | 4 | |a Immunoassay |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Viral Proteins |x chemistry |v Laboratory Manuals | |
| 650 | 4 | |a Viral Proteins |x genetics |v Laboratory Manuals | |
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Datensatz im Suchindex
| _version_ | 1804128595211190272 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
I GENOME AND TRANSCRIPT ANALYSES
1 Epstein Barr Virus: The B95 8 Strain Map
Paul J. Farrell 3
2 Analysis of Replication of oriP Based Plasmids
by Quantitative, Competitive PCR
Ann L. Kirchmaier 13
3 Genetic Analysis and Gene Expression
with Mini Epstein Barr Virus Plasmids
Ellen Kilgerand Wolfgang Hammerschmidt 23
4 Construction of cDNA Libraries for the Analysis of
the Structure of Complementary Strand Transcripts (CSTs)
Paul R. Smith 37
5 Analysis of the Expression and Function of the EBV Encoded
Small RNAs, the EBERs, in Heterologous Cells
Kenneth G. Laing, Volker Matys, and Michael J. Clemens 45
6 Visualizing EBV Expression Patterns by FISH
Anna Szeles 67
II VIRAL DETECTION
7 In Situ Detection of Epstein Barr Virus DNA
and Viral Gene Products
Gerald Niedobitek and Hermann Herbst 79
8 Phenotype Determination of Epstein Barr Virus Infected Cells
in Tissue Sections
Hermann Herbst and Gerald Niedobitek 93
9 Detection of EBV Infection at the Single Cell Level:
Precise Quantitation of Virus Infected Cells In Vivo
Gregory J. Babcock, Emily M. Miyashita Lin,
and David A. Thorley Lawson 103
vii
via Contents
10 Detection and Discrimination of Latent and Replicative
Herpesvirus Infection at the Single Cell Level In Vivo
Lisa L. Decker, Gregory J. Babcock,
and David A. Thorley Lawson 111
III CULTURE METHODS
11 Virus Isolation
LindseyM. Hutt Fletcher and Susan M. Turk 119
12 Generation of Lymphoblastoid Cell Lines (LCLs)
Teresa Frisan, Victor Levitsky, and Maria Masucci 125
13 Cell Cycle Distribution of B Lymphocytes and Cell Lines
Alison J. Sinclair 129
14 Introduction of Plasmid Vectors into Cells Via Electroporation
Ann L. Kirchmaier 137
15 Malignant Transformation and Immortalization Assays
in Animal Cells Transfected with the BARF1 Gene
Tadamasa Ooka 147
16 Transient Gene Expression and MACS Enrichment
Dieter Kube and Martina Vockerodt 155
17 In Vitro Assays to Study Epithelial Cell Growth
Christopher W. Dawson and Lawrence S. Young 165
18 In Vitro Assays to Study Epithelial Cell Differentiation
Christopher W. Dawson and Lawrence S. Young 173
19 Cell Sensitivity Assays: Quantitative Detection
ofApoptotic Cells In Vitro Using the TUNEL Assay
Neil A. Jones and Caroline Dive 181
20 Qualitative Detection of Apoptotic Cells Assessed
by DNA Fragmentation
Amanda J. Mcllwrath 189
IV IMMUNE ASSAYS
21 Regression Assay
Teresa Frisan, Victor Levitsky, and Maria Masucci 199
22 Generation of Polyclonal EBV Specific CTL Cultures and Clones
Victor Levitsky, Teresa Frisan, and Maria Masucci 203
23 Determination of Antigen and Fine Peptide Specificity
of EBV Specific CTLs
Victor Levitsky, Teresa Frisan, and Maria Masucci 209
24 Limiting Dilution Assay
Teresa Frisan, Victor Levitsky, and Maria Masucci 213
Contents jx
V VIRAL PROTEIN DETECTION
25 Antibodies for Detecting EBV Latent Proteins
Martin Rowe and Anja Mehl 219
26 Detection of EBV Latent Proteins by Western Blotting
Martin Rowe and Matthew Jones 229
27 Biosynthetic Radiolabeling of Virus Glycoproteins
for Immunoprecipitation and Electrophoretic Analysis
Lindsey M. Hutt Fletcher and Susan M. Turk 243
VI PROTEIN PROTEIN, PROTEIN DNA,
AND PROTEIN RNA INTERACTIONS
28 The Yeast Two Hybrid Assay to Identify Interacting Proteins
Kenneth M. Izumi 249
29 Identification of Transactivation, Repression, and Protein Protein
Interaction Domains Using GAL4 Fusion Proteins
James J. D. Hsieh and S. Diane Hayward 259
30 Magnetic DNA Affinity Purification of a Cellular Transcription Factor
Lothar J. Strobl and Ursula Zimber Strobl 277
31 Screening an Expression Library with a DNA Binding Site
Qin Zhang and Eduardo A. Montalvo 279
32 Analysis of DNA Binding Proteins by Mobility Shift Assay
Yi Chun James Wang and Eduardo A. Montalvo 289
33 Analysis of RNA Protein Interactions of the EBV Encoded
Small RNAs, the EBERs: In Vitro Assays
Kenneth G. Laing, Androulla Elia, Ian W. Jeffrey,
and Michael J. Clemens 297
VII PROTEIN ACTIVITY ASSAYS
34 Chimeric and Mutated Variants of LMP1: A Helpful Tool to Analyze
the Structure Function Relationship of a Pseudoreceptor
Olivier Gires, Marius Ueffing, and Wolfgang Hammerschmidt... 313
35 Assaying the Activity of Kinases Regulated by LMP1
ArndKieser 325
36 In Vitro Assays for the Detection of Protein Tyrosine
Phosphorylation and Protein Tyrosine Kinase Activities
Sara Fruehling and Richard Longnecker 337
x Contents
VIII TISSUE AND IN VIVO PROTOCOLS
37 Analysis of Apoptosis in Tissue Sections
Vicki Save, Philip J. Coates, and Peter A. Hall 347
38 Considerations in Generating Transgenic Mice: DNA, RNA, and
Protein Extractions from Tissues—Rapid and Effective Blotting
Joanna B. Wilson and Mark E. Drotar 367
39 In Vivo Assay of Cellular Proliferation
John Curran 379
40 Topical Chemical Carcinogen Treatment in Mice
John Curran 391
41 Separation of Epidermal Tissue from Underlying Dermis
and Primary Keratinocyte Culture
Jennifer Macdiarmid and Joanna B. Wilson 401
42 Selection and Enrichment of B Cells from Lymphoid Tissues
Penelope Tsimbouri, Marie Anne O Donnell,
and Joanna B. Wilson 411
43 Detection of Immunoglobulin Gene Rearrangements
Robert G. Caldwell and Richard Longnecker 423
Index 433
|
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| spelling | Epstein-Barr virus protocols ed. by Joanna B. Wilson ... Totowa, NJ Humana Press 2001 XIV, 438 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 174 Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Epstein-Barr virus Laboratory manuals Herpesvirus 4, Human isolation & purification Laboratory Manuals Herpesvirus 4, Human physiology Laboratory Manuals Immunoassay methods Laboratory Manuals Viral Proteins chemistry Laboratory Manuals Viral Proteins genetics Laboratory Manuals Virus Cultivation methods Laboratory Manuals Epstein-Barr-Virus-Infektion (DE-588)4265095-1 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Epstein-Barr-Virus (DE-588)4152573-5 gnd rswk-swf Labormedizin (DE-588)4073931-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Epstein-Barr-Virus (DE-588)4152573-5 s Molekularbiologie (DE-588)4039983-7 s DE-604 Epstein-Barr-Virus-Infektion (DE-588)4265095-1 s Labormedizin (DE-588)4073931-4 s Wilson, Joanna B. Sonstige oth Methods in molecular biology 174 (DE-604)BV035362695 174 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009415832&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Epstein-Barr virus protocols Methods in molecular biology Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Epstein-Barr virus Laboratory manuals Herpesvirus 4, Human isolation & purification Laboratory Manuals Herpesvirus 4, Human physiology Laboratory Manuals Immunoassay methods Laboratory Manuals Viral Proteins chemistry Laboratory Manuals Viral Proteins genetics Laboratory Manuals Virus Cultivation methods Laboratory Manuals Epstein-Barr-Virus-Infektion (DE-588)4265095-1 gnd Molekularbiologie (DE-588)4039983-7 gnd Epstein-Barr-Virus (DE-588)4152573-5 gnd Labormedizin (DE-588)4073931-4 gnd |
| subject_GND | (DE-588)4265095-1 (DE-588)4039983-7 (DE-588)4152573-5 (DE-588)4073931-4 (DE-588)4143413-4 |
| title | Epstein-Barr virus protocols |
| title_auth | Epstein-Barr virus protocols |
| title_exact_search | Epstein-Barr virus protocols |
| title_full | Epstein-Barr virus protocols ed. by Joanna B. Wilson ... |
| title_fullStr | Epstein-Barr virus protocols ed. by Joanna B. Wilson ... |
| title_full_unstemmed | Epstein-Barr virus protocols ed. by Joanna B. Wilson ... |
| title_short | Epstein-Barr virus protocols |
| title_sort | epstein barr virus protocols |
| topic | Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Epstein-Barr virus Laboratory manuals Herpesvirus 4, Human isolation & purification Laboratory Manuals Herpesvirus 4, Human physiology Laboratory Manuals Immunoassay methods Laboratory Manuals Viral Proteins chemistry Laboratory Manuals Viral Proteins genetics Laboratory Manuals Virus Cultivation methods Laboratory Manuals Epstein-Barr-Virus-Infektion (DE-588)4265095-1 gnd Molekularbiologie (DE-588)4039983-7 gnd Epstein-Barr-Virus (DE-588)4152573-5 gnd Labormedizin (DE-588)4073931-4 gnd |
| topic_facet | Wetenschappelijke technieken Wissenschaftliches Arbeiten Epstein-Barr virus Laboratory manuals Herpesvirus 4, Human isolation & purification Laboratory Manuals Herpesvirus 4, Human physiology Laboratory Manuals Immunoassay methods Laboratory Manuals Viral Proteins chemistry Laboratory Manuals Viral Proteins genetics Laboratory Manuals Virus Cultivation methods Laboratory Manuals Epstein-Barr-Virus-Infektion Molekularbiologie Epstein-Barr-Virus Labormedizin Aufsatzsammlung |
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