Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2000
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 79 Bl. Ill., graph. Darst. : 30 cm |
Internformat
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| 100 | 1 | |a Dejgaard, Kurt |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins |c by Kurt Dejgaard |
| 264 | 1 | |c 2000 | |
| 300 | |a 79 Bl. |b Ill., graph. Darst. : 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Heidelberg, Univ., Diss. | ||
| 650 | 7 | |a Hochschulschrift |2 gtt | |
| 650 | 0 | 7 | |a Coatomer |0 (DE-588)4518421-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Endoplasmatisches Retikulum |0 (DE-588)4152184-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Golgi-Apparat |0 (DE-588)4192795-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Endoplasmatisches Retikulum |0 (DE-588)4152184-5 |D s |
| 689 | 0 | 1 | |a Golgi-Apparat |0 (DE-588)4192795-3 |D s |
| 689 | 0 | 2 | |a Coatomer |0 (DE-588)4518421-5 |D s |
| 689 | 0 | |5 DE-604 | |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009406562 | |
Datensatz im Suchindex
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|---|---|
| adam_text |
TABLE OF CONTENTS
SUMMARY 4
ACKNOWLEDGEMENT 5
1. INTRODUCTION 9
1.1 THE EXOCYTIC PATHWAY 9
1.2 SORTING AND TRANSPORT MACHINERY: "COATS" 10
1.3 COP II
N
1.3.1 TRANSPORT AND GENETIC DEFECTS 11
1.3.2 RECONSTITUTED COP II MINIMAL MACHINERY 12
1.3.3 COP II, FUNCTIONAL ASPECTS. 13
1.4. COP I
15
1.4.1 COP I PROTEIN COMPLEX AND ASSOCIATED PROTEINS 15
1.4.2 RECONSTITUTED COP I MINIMAL MACHINERY ASSAYS 17
1.4.3 COP I, FUNCTIONAL ASPECTS (TRANSPORT PARTNERS) 18
1.4.4. COP I, FUNCTIONAL ASPECTS (TRANSPORT VESICLES
IN VITRO) 20
1.4.5. COP I,'FUNCTIONAL ASPECTS (TRANSPORT
IN VIVO) 21
1.4.6 COP I, FUNCTIONAL ASPECTS (GOLGI STRUCTURAL INTEGRITY) 23
1.5 OUTLINE OF PROJECT 25
6
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/961193905
2. RESULTS
28
2.1 IN VITRO AND IN VIVO ACTIVITIES OF K(X)KXX AND AROMATIC
MOTIFS OF THE SMALL TRANSMEMBRANE PROTEIN P24 FAMILY 28
2.2 CHARACTERIZATION OF A NOVEL MOTIF SHARED BETWEEN THE P24
Y SUB-FAMILY, RER1 AND THE KDEL RECEPTOR ERD2P 34
2.3 IN VITRO AND IN VIVO ACTIVITIES OF THE POSITIVELY CHARGED SEMI-MOTIF
OF
CYTOPLASMIC DOMAINS OF TYPE II TRANSMEMBRANE GOLGI RESIDENT
GLYCOSYLTRANSFERASES 39
3. DISCUSSION 50
3.1 COP II MOTIFS: 50
3.2 COP I/CYTOPLASMIC DOMAIII INTERACTIONS 52
3.3 COP I MOTIFS ON REPORTER MOLECULES 55
3.4 COP I MOTIFS ON THEIR MOLECULES OF ORIGIN 58
4. MATERIALS AND METHODS 61
4.1 GENERAL 61
4.1.1 ANTIBODIES, PROTEINS AND VECTORS 61
4.1.2 CELL CULTURE AND-EXTRACTS 63
4.1.3 CELL TRANSFECTION 64
4.1.4 [
35S]-METHIONINE LABELING AND IMMUNOPRECIPITATION 65
4.1.5 IMMUNOFLUORESCENCE LABELING 66
4.1.6 SODIUM DODECYL SULPHATE ELECTROPHORESIS, FLUOROGRAPHY AND WESTERN
BLOTTING 66
4.2 PEPTIDE INTERACTION ASSAY 67
4.2.1 PEPTIDE COUPLING 67
4.2.2 PROTEIN INCUBATION AND ASSAY 68
7
4.3 FLUORESCENCE CORRELATION SPECTROSCOPY 69
4.3.1 FLUOROPHORE COUPLING AND PURIFICATION 70
4.3.2 ONE COMPONENT MEASUREMENT/CALIBRATION 70
4.3.3 TWO COMPONENT MEASUREMENT 71
4.3.4 AFFINITY CALCULATIONS 72
5. REFERENCES 73
8 |
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| indexdate | 2024-10-09T18:09:40Z |
| institution | BVB |
| language | English |
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| spelling | Dejgaard, Kurt Verfasser aut Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins by Kurt Dejgaard 2000 79 Bl. Ill., graph. Darst. : 30 cm txt rdacontent n rdamedia nc rdacarrier Heidelberg, Univ., Diss. Hochschulschrift gtt Coatomer (DE-588)4518421-5 gnd rswk-swf Endoplasmatisches Retikulum (DE-588)4152184-5 gnd rswk-swf Golgi-Apparat (DE-588)4192795-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Endoplasmatisches Retikulum (DE-588)4152184-5 s Golgi-Apparat (DE-588)4192795-3 s Coatomer (DE-588)4518421-5 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009406562&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Dejgaard, Kurt Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins Hochschulschrift gtt Coatomer (DE-588)4518421-5 gnd Endoplasmatisches Retikulum (DE-588)4152184-5 gnd Golgi-Apparat (DE-588)4192795-3 gnd |
| subject_GND | (DE-588)4518421-5 (DE-588)4152184-5 (DE-588)4192795-3 (DE-588)4113937-9 |
| title | Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins |
| title_auth | Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins |
| title_exact_search | Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins |
| title_full | Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins by Kurt Dejgaard |
| title_fullStr | Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins by Kurt Dejgaard |
| title_full_unstemmed | Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins by Kurt Dejgaard |
| title_short | Identification and characterization of coatomer binding motifs in cytoplasmic domains of ERGIC and Golgi proteins |
| title_sort | identification and characterization of coatomer binding motifs in cytoplasmic domains of ergic and golgi proteins |
| topic | Hochschulschrift gtt Coatomer (DE-588)4518421-5 gnd Endoplasmatisches Retikulum (DE-588)4152184-5 gnd Golgi-Apparat (DE-588)4192795-3 gnd |
| topic_facet | Hochschulschrift Coatomer Endoplasmatisches Retikulum Golgi-Apparat |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009406562&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT dejgaardkurt identificationandcharacterizationofcoatomerbindingmotifsincytoplasmicdomainsofergicandgolgiproteins |