Nuclease methods and protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Schriftenreihe: | Methods in molecular biology
160 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVII, 525 S. Ill., graph. Darst. |
| ISBN: | 0896036790 |
Internformat
MARC
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| 245 | 1 | 0 | |a Nuclease methods and protocols |c ed. by Catherine H. Schein |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2001 | |
| 300 | |a XVII, 525 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in molecular biology |v 160 | |
| 650 | 4 | |a Nucléases - Manuels de laboratoire | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Deoxyribonucleases |v Laboratory Manuals | |
| 650 | 4 | |a Endonucleases |v Laboratory Manuals | |
| 650 | 4 | |a Nucleases |v Laboratory manuals | |
| 650 | 4 | |a Ribonucleases |v Laboratory Manuals | |
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Datensatz im Suchindex
| _version_ | 1804128499313672192 |
|---|---|
| adam_text | Contents
Preface v
List of Abbreviations ix
List of Contributors xv
Part I. Characterizing Nuclease Activity 7
1 Processivity of DNA Repair Enzymes
R. Stephen Lloyd 3
2 Analysis by HPLC of Distributive Activities and the Synthetic (Back) Reaction
of Pancreatic Type Ribonucleases
M. Victoria Nogues and Claudi M. Cuchillo 15
3 Irelp: A Kinase and Site Specific Endoribonuclease
Tania N. Gonzalez and Peter Walter 25
4 Microtiter Plate Assay and Related Assays for Nonspecific Endonucleases
Gregor Meiss, Oleg Gimadutdinow, Peter Friedhoff,
and Alfred M. Pingoud 37
5 Quantitating mRNAs with Relative and Competitive RT PCR
Ellen A. Prediger 49
6 Expressing Self Incompatibility RNases (S RNases) in Transgenic Plants
Brian Beecher and Bruce A. McClure 65
7 Molecular Cloning, Tissue Distribution, and Chromosomal Localization
of the Human Homolog of the R2/Th/Stylar Ribonuclease Gene Family
Francesco Acquati, Clnzia Nucci, Marco G. Bianchi, Tatiana Gorletta,
and Roberto Taramelli 87
Part II. Inhibitors and Activators of Nucleases 103
8 Ribonuclease Inhibitors
BrittanL. Pasloske 105
9 Producing Soluble Recombinant RNases and Assays to Measure Their
Interaction with Interferon y In Vitro
Catherine H. Schein 113
10 Assays for the Evaluation of HIV 1 Integrase Inhibitors
Zeger Debyser, Peter Cherepanov, Wim Pluymers,
and Erik De Clercq 139
11 The Direction of Ribonucleases H by Antisense Oligodeoxynucleotides
Richard V. Giles and David M. Tidd 157
12 The 2 5A/RNase L Pathway and Inhibition by RNase L Inhibitor (RLI)
Catherine Bisbal, Tamim Salehzada, Michelle Silhol,
Camille Martinand, Florence Le Roy, and Bernard Lebleu 183
xi
xii Contents
Part III. Relating Nuclease Structure and Function 199
13 Crystallization and Crystal Structure Determination of Ribonuclease
A Ribonuclease Inhibitor Protein Complex
BostjanKobe 207
14 Methods for Studying the Interaction of Barnase with Its Inhibitor Barstar
Gideon Schreiber 213
15 Assaying In Vitro Refolding of RNases by Mass Spectrometry
Gennaro Marino, Piero Pucci, and Margherita Ruoppolo 227
16 Dissecting Nucleases into Their Structural and Functional Domains:
Mapping the RNA Binding Surface of RNase III by NMR
Andres Ramos and Annalisa Pastore 237
17 Using Electrostatics to Define the Active Site of Serratia Endonuclease
Kurt L. Krause and Mitchell D. Miller 249
18 Homology Modeling and Simulations of Nuclease Structures
Kizhake V. Soman, Catherine H. Schein, Hongyao Zhu,
and Werner Braun 263
19 Methods for Determining Activity and Specificity of DNA Binding and DNA
Cleavage by Class II Restriction Endonucleases
Albert Jeltsch and Alfred M. Pingoud 287
20 Engineered Properties and Assays for Human DNase I Mutants
Clark Q. Pan, Dominick V. Sinicropi, and Robert A. Lazarus 309
Part IV. Nucleases in the Clinic 323
21 Assays for Human DNase I Activity in Biological Matrices
Dominick V. Sinicropi and Robert A. Lazarus 325
22 Evaluation of Retroviral Ribonuclease H Activity
Jennifer T. Miller, Jason W. Rausch, and Stuart F. J. Le Grice 335
23 Assays for Detection of RNase A Superfamily Ribonucleases
Helene F. Rosenberg and Joseph B. Domachowske 355
24 Assay for Antitumor and Lectin Activity in RNase Homologs
KazuoNitta 363
25 Isolation and Enzymatic Activity of Angiogenin
James F. Riordan and Robert Shapiro 375
26 Preparation and Preclinical Characterization of RNase Based Immunofusion
Proteins
Dianne L. Newton and Susanna M. Rybak 387
Part V. Assays Using Nucleases 407
27 Restriction Endonucleases and Their Uses
Raymond J. Williams 409
28 Isolation and Characterization of an Unknown Restriction Endonuclease
Raymond J. Williams 431
29 Gene Modification with Hapaxoterministic Restriction Enzymes:
Easing the Way
Shelby L. Berger 443
Contents xiii
30 Techniques to Measure Nucleic Acid Protein Binding and Specificity:
Nuclear Extract Preparations, DNase I Footprinting, and Mobility
Shift Assays
Richard A. Rippe, David A. Brenner, and Antonio Tugores 459
31 Analyzing the Developmental Expression of Sigma Factors with S1 Nuclease
Mapping
Jan Kormanec 481
32 Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays
Ellen A. Prediger 495
Index 507
|
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| isbn | 0896036790 |
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| spelling | Nuclease methods and protocols ed. by Catherine H. Schein Totowa, NJ Humana Press 2001 XVII, 525 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 160 Nucléases - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Deoxyribonucleases Laboratory Manuals Endonucleases Laboratory Manuals Nucleases Laboratory manuals Ribonucleases Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Nucleasen (DE-588)4172116-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Nucleasen (DE-588)4172116-0 s DE-604 Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s Schein, Catherine H. Sonstige (DE-588)136346030 oth Methods in molecular biology 160 (DE-604)BV035362695 160 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009351726&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Nuclease methods and protocols Methods in molecular biology Nucléases - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Deoxyribonucleases Laboratory Manuals Endonucleases Laboratory Manuals Nucleases Laboratory manuals Ribonucleases Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Nucleasen (DE-588)4172116-0 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4172116-0 (DE-588)4038971-6 (DE-588)4143413-4 |
| title | Nuclease methods and protocols |
| title_auth | Nuclease methods and protocols |
| title_exact_search | Nuclease methods and protocols |
| title_full | Nuclease methods and protocols ed. by Catherine H. Schein |
| title_fullStr | Nuclease methods and protocols ed. by Catherine H. Schein |
| title_full_unstemmed | Nuclease methods and protocols ed. by Catherine H. Schein |
| title_short | Nuclease methods and protocols |
| title_sort | nuclease methods and protocols |
| topic | Nucléases - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Deoxyribonucleases Laboratory Manuals Endonucleases Laboratory Manuals Nucleases Laboratory manuals Ribonucleases Laboratory Manuals Molekularbiologie (DE-588)4039983-7 gnd Nucleasen (DE-588)4172116-0 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Nucléases - Manuels de laboratoire Wetenschappelijke technieken Wissenschaftliches Arbeiten Deoxyribonucleases Laboratory Manuals Endonucleases Laboratory Manuals Nucleases Laboratory manuals Ribonucleases Laboratory Manuals Molekularbiologie Nucleasen Methode Aufsatzsammlung |
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