Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas: fluorescence imaging, spectral analysis and kinetics
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
München
Hieronymus
2000
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| Schriftenreihe: | Medizin
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: München, Univ., Med. Fak., Diss., 2000 |
| Beschreibung: | 142 S. Ill., graph. Darst. |
| ISBN: | 389791171X |
Internformat
MARC
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| 100 | 1 | |a Betz, Christian |d 1972- |e Verfasser |0 (DE-588)130227781 |4 aut | |
| 245 | 1 | 0 | |a Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas |b fluorescence imaging, spectral analysis and kinetics |c Christian Betz |
| 264 | 1 | |a München |b Hieronymus |c 2000 | |
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Datensatz im Suchindex
| _version_ | 1804128448363364352 |
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| adam_text | Table of contents
I INTRODUCTION 1
1 ORAL AND OROPHARYNGEAL CANCER 1
1.1 EPIDEMIOLOGICAL FACTS 1
1.2 Etiology of head and neck cancer (HNC) 3
1.3 Secondary tumors and field cancerization 4
1.4 Diagnostic approach to head and neck cancers 4
1.5 Management of head and neck cancers 4
1.6 the value of early detection 5
1.7 The value of exact demarcation 5
2 novel techniques for tumor demarcation 6
2.1 Dyes and stains 6
2.1.1 lugol s iodine 6
2.1.2 toluidine blue 6
2.2 exogenously applied fluorescent markers 7
2.2.1 hematoporphyrin derivatives 7
2.2.2 tetracyclines 7
2.2.3 5 aminolevulinic induced protoporphyrin ix 7
2.3 Endogenous autofluorescence 9
2.3.1 Endogenous porphyrins 9
2.3.2 Proper tissue autofluorescence 9
3 Principles of combined fluorescence diagnosis (CFD) 11
3.1 PROTOPORPHYRIN IX (PPIX) FLUORESCENCE 11
..1,1 Basic principles of fluorescence at the example of the PPIX molecule .... 11
3.1.2 porphyrins and biosynthesis of heme 13
3.1.3 Disturbances of the physiologic heme biosynthesis 14
: 3.1.4 Exogenous administration of 5 ALA 15
3.1.5 Tumor selectivity of 5 ALA induced PPIX fluorescence 16
3.2 Autofluorescence 18
! 3.2.1 basic principles on tissue autofluorescence 18
3.2.2 Tumor selectivity of tissue autofluorescence 20
4 Objective of the study 23
II MATERIALS AND METHODS 24
1 Selection of patients 24
2 Medication and dosing of 5 ALA 25
| 3 Experimental setup 25
j 3.1 Modified Endoscope 26
3.2 Advanced excitation / detection unit 26
3.3 Light source 26
3.4 Camera and Video Documentation 27
3.5 Modified surgical microscope 27
4 Fluorescence imaging 29
4.1 Imaging of PPIX Fluorescence 29
4.2 Autofluorescence imaging 29
: 4.3 Combined fluorescence diagnosis (CFD) 30
5 Spectral data analysis 32
5.1 Measurements on the patients 32
5.2 Reproducible acquirement of data 32
5.3 Description of detector units 33
5.4 detector noise and statistical errors 35
5.5 Calibration with a fluorescence standard 35
5.6 Reproducible analysis of data 35
6 Kinetics of 5 ALA induced PPIX 36
6.1 Patients 36
6.2 Determination of PPIX in human blood plasma 36
6.2.1 Collection of plasma samples 36
6.2.2 Spectral measurements of human blood plasma 37
6.2.3 Determination of PPIX concentrations in human plasma samples 37
6.2.4 Graphical presentation and illustration of results 38
6.3 Determination of PPIX in human skin 39
III RESULTS 40
1 analyzed data on selected patients 40
2 Fluorescence imaging 43
2.1 Imaging of PPIX fluorescence 43
2.2 Imaging of autofluorescence 43
2.2.1 General information concerning patients and method 43
2.2.2 Fluorescence imaging 44
2.2.3 subjective demarcation properties of autofluorescence 46
2.2.4 Evaluation of biopsies 50
2.3 Combined fluorescence detection (CFD) 52
2.3.1 General information concerning patients and method 52
2.3.2 Fluorescence imaging 52
2.3.3 Subjective demarcation properties of CFD 55
2.3.4 Evaluation of biopsies 59
2.3.5 Estimated clinical benefit 61
2.4 Comparison of normal inspection, autofluorescence diagnosis and CFD 62
3 Spectral data analysis 63
3.1 Analysis of PPIX fluorescence 64
3.2 Analysis of autofluorescence 70
3.3 cfd (combined fluorescence diagnosis) 78
4 Kinetics of 5 ALA induced PPIX 81
4.1 ppix in human blood plasma 81
4.2 ppix in human skin 82
iv discussion 83
1 Materials and Methods 83
1.1 Selection of patients 83
1.2 5 aminolevulinic acid 83
1.3 Experimental setup 84
1.3.1 Excitation / detection unit 84
1.3.2 Light source 86
1.4 Fluorescence imaging 86
1.5 Spectral data analysis 87
1.6 Kinetics of 5 ALA induced PPIX 88
2 Results 89
2.1 Patients 89
2.2 Fluorescence imaging 90
2.2.1 Autofluorescence imaging 90
2.2.1.1 General statements on the results of autofluorescence imaging 90
2.2.1.2 Subjective demarcation properties of autofluorescence imaging 91
2.2.1.3 Evaluation of biopsies 92
2.2.1.4 Comparison to other methods of autofluorescence imaging in ENT.... 93
2.2.1.5 THE VALUE OF ENDOGENOUS PORPHYRINS IN AUTOFLUORESCENCE IMAGING 94
2.2.2 Combined fluorescence diagnosis (CFD) 95
2.2.2.1 General statements on the results of CFD 95
2.2.2.2 Subjective demarcation properties of CFD 96
2.2.2.3 Evaluation of biopsies 97
2.2.2.4 Estimated clinical benefit from CFD 98
2.2.2.5 Comparison of CFD within the field of otorhinolaryngology 98
2.2.2.6 Comparison of CFD for various fields of medicine 100
2.2.2.7 Comparison of CFD to other novel techniques for tumor detection. 102
2.3 Spectral data analysis 104
2.3.1 Analysis of PPIX fluorescence 104
2.3.2 Analysis of autofluorescence 106
2.3.2.1 Own observations in autofluorescence spectroscopy 106
2.3.2.2 Comparison to other methods of autofluorescence spectroscopy .. 108
2.3.2.3 Endogenous porphyrins in autofluorescence spectroscopy 109
2.3.3 Analysis of CFD (Combined fluorescence diagnosis) 110
2.3.3.1 Own observations in CFD spectroscopy 110
2.3.3.2 Comparison to other methods of CFD spectroscopy in ENT 111
2.4 Kinetics of 5 ALA induced PPIX 111
V CONCLUSION 113
VI CONCLUSION (GERMAN TRANSLATION) 117
VI REFERENCE LIST 121
VII ACKNOWLEDGEMENTS 143
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| indexdate | 2024-07-09T18:49:24Z |
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| isbn | 389791171X |
| language | English |
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| spelling | Betz, Christian 1972- Verfasser (DE-588)130227781 aut Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas fluorescence imaging, spectral analysis and kinetics Christian Betz München Hieronymus 2000 142 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Medizin Zugl.: München, Univ., Med. Fak., Diss., 2000 Hochschulschrift gtt (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009318539&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Betz, Christian 1972- Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas fluorescence imaging, spectral analysis and kinetics Hochschulschrift gtt |
| subject_GND | (DE-588)4113937-9 |
| title | Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas fluorescence imaging, spectral analysis and kinetics |
| title_auth | Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas fluorescence imaging, spectral analysis and kinetics |
| title_exact_search | Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas fluorescence imaging, spectral analysis and kinetics |
| title_full | Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas fluorescence imaging, spectral analysis and kinetics Christian Betz |
| title_fullStr | Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas fluorescence imaging, spectral analysis and kinetics Christian Betz |
| title_full_unstemmed | Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas fluorescence imaging, spectral analysis and kinetics Christian Betz |
| title_short | Combined fluorescence diagnosis (5-ALA induced PPIX and autofluorescence) for the detection and delimitation of oral carcinomas |
| title_sort | combined fluorescence diagnosis 5 ala induced ppix and autofluorescence for the detection and delimitation of oral carcinomas fluorescence imaging spectral analysis and kinetics |
| title_sub | fluorescence imaging, spectral analysis and kinetics |
| topic | Hochschulschrift gtt |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009318539&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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