Matrix metalloproteinase protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Schriftenreihe: | Methods in molecular biology
151 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 545 S. Ill., graph. Darst. |
| ISBN: | 0896037339 |
Internformat
MARC
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| 245 | 1 | 0 | |a Matrix metalloproteinase protocols |c ed. by Ian M. Clark |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2001 | |
| 300 | |a XV, 545 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 151 | |
| 650 | 4 | |a Biochemistry - Technique | |
| 650 | 7 | |a Extracellulaire matrix |2 gtt | |
| 650 | 7 | |a Laboratoriumonderzoek |2 gtt | |
| 650 | 4 | |a Metalloproteinases | |
| 650 | 7 | |a Metalloproteïnases |2 gtt | |
| 650 | 7 | |a Metaloproteinases |2 larpcal | |
| 650 | 4 | |a Proteolytic enzymes | |
| 650 | 4 | |a Extracellular matrix proteins |v Laboratory manuals | |
| 650 | 4 | |a Matrix Metalloproteinases |v Laboratory Manuals | |
| 650 | 4 | |a Metalloproteinases |v Laboratory manuals | |
| 650 | 0 | 7 | |a Grundsubstanz |0 (DE-588)4224942-9 |2 gnd |9 rswk-swf |
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| 650 | 0 | 7 | |a Metalloproteinasen |0 (DE-588)4284137-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
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| 700 | 1 | |a Clark, Ian M. |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 151 |w (DE-604)BV035362695 |9 151 | |
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Datensatz im Suchindex
| _version_ | 1804128423014039552 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I. MMPs and TIMPs—An Overview of the Field
1 MMPs and TIMPs:
An Historical Perspective
J. Frederick Woessner, Jr. 1
2 Strategies for Cloning New MMPs and TIMPs
Gloria Velasco and Carlos Ldpez Otin 25
3 Structural Studies on MMPs and TIMPs
Wolfram Bode and Klaus Maskos 45
4 Matrix Metalloproteinase Substrate Binding Domains,
Modules and Exosites:
Overview and Experimental Strategies
Christopher M. Overall 79
5 The Matrix Metalloproteinase (MMP) and Tissue Inhibitor
of Metalloproteinase (TIMP) Genes:
Transcriptional and Posttranscriptional Regulation,
Signal Transduction and Cell Type Specific Expression
Matthew P. Vincenti 121
6 Models for Gain of Function and Loss of Function of MMPs:
Transgenic and Gene Targeted Mice
Lisa M. Coussens, Steven D. Shapiro, Paul D. Soloway,
andZenaWerb 149
Part II. Expression and Purification of MMPs and TIMPs
7 Expression of MMPs and TIMPs in Mammalian Cells
Karen M. L. Yeow, Blaine W. Phillips, P. Paul Beaudry,
Kevin J. Leco, Gillian Murphy, and Dylan R. Edwards 181
8 Expression of Recombinant Matrix Metalloproteinases
in Escherichia coli
L. Jack Windsor and Darin L. Steele 191
vii
9 Expression of Human Collagenase I (MMP 1) and TIMP 1
in a Baculovirus Based Expression System
Riidiger Vallon and Peter Angel 207
10 Expression of Recombinant Matrix Metalloproteinases in Yeast
Glenn A. Doyle 219
11 Expression of Recombinant Membrane Type MMPs
Michael J. Butler, Marie Pia d Ortho,
and Susan J. Atkinson 239
12 Refolding of TIMP 2 from Escherichia coli Inclusion Bodies
Richard A. Williamson 257
13 Expression and Refolding of Full Length Human TIMP 1
Deborah Davis 267
14 Purification of MMPs and TIMPs
Ken ichi Shimokawa and Hideaki Nagase 275
Part III. Detection of MMPs and TIMPs
15 Monitoring MMP and TIMP mRNA Expression by RT PCR
Howard Wong, Huong Muzik, Lori L. Groft, Marc A. Lafleur,
Charles Matouk, Peter A. Forsyth, Gilbert A. Schultz,
Steven J. Wall, and Dylan R. Edwards 305
16 Measuring Transcription of Metalloproteinase Genes:
Nuclear Run Off Assay vs Analysis of hnRNA
Anne M. Delany 321
17 In Situ Hybridization for Metalloproteinases and Their Inhibitors
Tina L. Hurskainen and Suneel S. Apte 335
18 Use of EIA to Measure MMPs and TIMPs
Noboru Fujimoto and Kazushi Iwata 347
19 Immunohistochemistry of MMPs and TIMPs
Yasunori Okada 359
20 Detecting Polymorphisms in MMP Genes
Shu Ye and Adriano M. Henney 367
Part IV. Assay of MMP and TIMP Activities
21 Methods for Studying Activation of Matrix Metalloproteinases
Vera Knauperand Gillian Murphy 377
22 Assay of Matrix Metalloproteinases Against Matrix Substrates
Timothy E. Cawston, Paul Koshy,
and Andrew D. Rowan 389
23 Zymography and Reverse Zymography for Detecting
MMPs and TIMPs
Susan P. Hawkes, Hongxia Li, and Gary T. Taniguchi 399
24 In Situ Zymography
Sarah J. George and Jason L Johnson 411
25 Detection of Focal Proteolysis Using Texas Red Gelatin
Rosalind M. Hembry 417
26 Antibodies to MMP Cleaved Aggrecan
Amanda J. Fosang, Karena Last, David C. Jackson,
and Lorena Brown 425
27 Cartilage Proteoglycan Release Assay
CaronJ. Billington 451
28 Immunoassay for Collagenase Mediated Cleavage
of Types I and II Collagens
R. Clark Billinghurst, Mirela lonescu,
and A. Robin Poole 457
29 Collagen Degradation Assays
Anthony P. Hollander 473
30 Invasion Assays and Matrix Metalloproteinases:
Quantification of Cellular Invasion Using Propidium
Iodide Labeling and Confocal Laser Scanning Microscopy
Ulrike Benbow, Kenneth A. Orndorff, and Alice L. Givan 485
31 Using Fluorogenic Peptide Substrates to Assay
Matrix Metalloproteinases
Gregg B. Fields 495
32 Kinetic Analysis of the Inhibition of Matrix Metalloproteinases
by Tissue Inhibitor of Metalloproteinases (TIMP)
Mike Hutton and Frances Willenbrock 519
33 Assaying Growth Factor Activity of Tissue Inhibitors
of Metalloproteinases
Kyoko Yamashita and Taro Hayakawa 533
Index 539
|
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| id | DE-604.BV013614867 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:49:00Z |
| institution | BVB |
| isbn | 0896037339 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009301799 |
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| physical | XV, 545 S. Ill., graph. Darst. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Matrix metalloproteinase protocols ed. by Ian M. Clark Totowa, NJ Humana Press 2001 XV, 545 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 151 Biochemistry - Technique Extracellulaire matrix gtt Laboratoriumonderzoek gtt Metalloproteinases Metalloproteïnases gtt Metaloproteinases larpcal Proteolytic enzymes Extracellular matrix proteins Laboratory manuals Matrix Metalloproteinases Laboratory Manuals Metalloproteinases Laboratory manuals Grundsubstanz (DE-588)4224942-9 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Metalloproteinasen (DE-588)4284137-9 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Metalloproteinasen (DE-588)4284137-9 s Grundsubstanz (DE-588)4224942-9 s DE-604 Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s Clark, Ian M. Sonstige oth Methods in molecular biology 151 (DE-604)BV035362695 151 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009301799&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Matrix metalloproteinase protocols Methods in molecular biology Biochemistry - Technique Extracellulaire matrix gtt Laboratoriumonderzoek gtt Metalloproteinases Metalloproteïnases gtt Metaloproteinases larpcal Proteolytic enzymes Extracellular matrix proteins Laboratory manuals Matrix Metalloproteinases Laboratory Manuals Metalloproteinases Laboratory manuals Grundsubstanz (DE-588)4224942-9 gnd Molekularbiologie (DE-588)4039983-7 gnd Metalloproteinasen (DE-588)4284137-9 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4224942-9 (DE-588)4039983-7 (DE-588)4284137-9 (DE-588)4038971-6 |
| title | Matrix metalloproteinase protocols |
| title_auth | Matrix metalloproteinase protocols |
| title_exact_search | Matrix metalloproteinase protocols |
| title_full | Matrix metalloproteinase protocols ed. by Ian M. Clark |
| title_fullStr | Matrix metalloproteinase protocols ed. by Ian M. Clark |
| title_full_unstemmed | Matrix metalloproteinase protocols ed. by Ian M. Clark |
| title_short | Matrix metalloproteinase protocols |
| title_sort | matrix metalloproteinase protocols |
| topic | Biochemistry - Technique Extracellulaire matrix gtt Laboratoriumonderzoek gtt Metalloproteinases Metalloproteïnases gtt Metaloproteinases larpcal Proteolytic enzymes Extracellular matrix proteins Laboratory manuals Matrix Metalloproteinases Laboratory Manuals Metalloproteinases Laboratory manuals Grundsubstanz (DE-588)4224942-9 gnd Molekularbiologie (DE-588)4039983-7 gnd Metalloproteinasen (DE-588)4284137-9 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Biochemistry - Technique Extracellulaire matrix Laboratoriumonderzoek Metalloproteinases Metalloproteïnases Metaloproteinases Proteolytic enzymes Extracellular matrix proteins Laboratory manuals Matrix Metalloproteinases Laboratory Manuals Metalloproteinases Laboratory manuals Grundsubstanz Molekularbiologie Metalloproteinasen Methode |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009301799&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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