DNA sequencing protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
167 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Inhaltsverzeichnis |
| Beschreibung: | X, 244 S. graph. Darst. |
| ISBN: | 0896037169 0896037215 |
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| 245 | 1 | 0 | |a DNA sequencing protocols |c ed. by Colin A. Graham ... |
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Datensatz im Suchindex
| _version_ | 1804128396720996352 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
1 Introduction to DNA Sequencing
Colin A. Graham and Alison J. M. Hill 1
2 The Universal Primers and the Shotgun DNA Sequencing Method
Joachim Messing 13
3 M13 Sequencing
Qingzhong Yu 33
4 Primer Design and Primer-Directed Sequencing
Ulrike Gerischer and Peter Durre 39
5 Direct Sequencing of DNA Produced in a Polymerase Chain Reaction
Ulrike Gerischer 53
6 Solid Phase Fluorescent Sequencing of the CFTR Gene
Harry Cuppens and Jean-Jacques Cassiman 63
7 Shotgun DNA Sequencing
Alan T. Bankier 89
8 Cycle Sequencing
Robert W. Blakesley 101
9 Cycle Sequencing of Polymerase Chain Reaction-Amplified
Genomic DNA with Dye-Labeled Universal Primers
Johannes Zschocke and Georg F. Hoffmann 113
10 Automated Fluorescent DNA Sequencing on the ABI PRISM 377
Jonathan R. E. MacBeath, Suzanna S. Harvey,
and Nicola J. Oldroyd 119
11 Automated Fluorescent DNA Sequencing on the ABI PRISM 310
Genetic Analyzer
David Watts and Jonathan R. E. MacBeath 153
12 Fluorescent Sequencing Protocols for the ALF
Shirley McQuaid 171
13 Fluorescent Sequencing for Heterozygote Mutation Detection
Colin A. Graham and Alison J. M. Hill 193
vii
viii Contents
14 Sequence Databases and the Internet
Anne E. Hughes 215
15 DNA Sequencing by Capillary Array Electrophoresis
Norman J. Dovichi and JianZhong Zhang 225
Index 241
Contents
Preface v
Contributors ix
1 Introduction to DNA Sequencing
Colin A. Graham and Alison J. M. Hill 1
2 The Universal Primers and the Shotgun DNA Sequencing Method
Joachim Messing 13
3 M13 Sequencing
Qingzhong Yu 33
4 Primer Design and Primer Directed Sequencing
Ulrike Gerischer and Peter Durre 39
5 Direct Sequencing of DNA Produced in a Polymerase Chain Reaction
Ulrike Gerischer 53
6 Solid Phase Fluorescent Sequencing of the CFTR Gene
Harry Cuppens and Jean Jacques Cassiman 63
7 Shotgun DNA Sequencing
Alan T. Bankier 89
8 Cycle Sequencing
Robert W. Blakesley 101
9 Cycle Sequencing of Polymerase Chain Reaction Amplified
Genomic DNA with Dye Labeled Universal Primers
Johannes Zschocke and Georg F. Hoffmann 113
10 Automated Fluorescent DNA Sequencing on the ABI PRISM 377
Jonathan R. E. MacBeath, Suzanna S. Harvey,
and Nicola J. Oldroyd 119
11 Automated Fluorescent DNA Sequencing on the ABI PRISM 310
Genetic Analyzer
David Watts and Jonathan R. E. MacBeath 153
12 Fluorescent Sequencing Protocols for the ALF
Shirley McQuaid 171
13 Fluorescent Sequencing for Heterozygote Mutation Detection
Colin A. Graham and Alison J. M. Hill 193
vii
viii Contents
14 Sequence Databases and the Internet
Anne E. Hughes 215
15 DNA Sequencing by Capillary Array Electrophoresis
Norman J. Dovichi and JianZhong Zhang 225
Index 241
|
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| edition | 2. ed. |
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| spelling | DNA sequencing protocols ed. by Colin A. Graham ... 2. ed. Totowa, NJ Humana Press 2001 X, 244 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 167 ADN - Manuels de laboratoire Séquence nucléotidique - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Nucleotide sequence Laboratory manuals Sequence Analysis, DNA Laboratory Manuals Methode (DE-588)4038971-6 gnd rswk-swf DNS-Sequenz (DE-588)4150352-1 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf DNS (DE-588)4070512-2 gnd rswk-swf Sequenzanalyse Chemie (DE-588)4132277-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content DNS (DE-588)4070512-2 s Sequenzanalyse Chemie (DE-588)4132277-0 s Methode (DE-588)4038971-6 s DE-604 DNS-Sequenz (DE-588)4150352-1 s Labortechnik (DE-588)4123602-6 s Graham, Colin A. Sonstige oth Methods in molecular biology 167 (DE-604)BV035362695 167 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009284451&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009284451&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | DNA sequencing protocols Methods in molecular biology ADN - Manuels de laboratoire Séquence nucléotidique - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Nucleotide sequence Laboratory manuals Sequence Analysis, DNA Laboratory Manuals Methode (DE-588)4038971-6 gnd DNS-Sequenz (DE-588)4150352-1 gnd Labortechnik (DE-588)4123602-6 gnd DNS (DE-588)4070512-2 gnd Sequenzanalyse Chemie (DE-588)4132277-0 gnd |
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| title | DNA sequencing protocols |
| title_auth | DNA sequencing protocols |
| title_exact_search | DNA sequencing protocols |
| title_full | DNA sequencing protocols ed. by Colin A. Graham ... |
| title_fullStr | DNA sequencing protocols ed. by Colin A. Graham ... |
| title_full_unstemmed | DNA sequencing protocols ed. by Colin A. Graham ... |
| title_short | DNA sequencing protocols |
| title_sort | dna sequencing protocols |
| topic | ADN - Manuels de laboratoire Séquence nucléotidique - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Nucleotide sequence Laboratory manuals Sequence Analysis, DNA Laboratory Manuals Methode (DE-588)4038971-6 gnd DNS-Sequenz (DE-588)4150352-1 gnd Labortechnik (DE-588)4123602-6 gnd DNS (DE-588)4070512-2 gnd Sequenzanalyse Chemie (DE-588)4132277-0 gnd |
| topic_facet | ADN - Manuels de laboratoire Séquence nucléotidique - Manuels de laboratoire Wetenschappelijke technieken Wissenschaftliches Arbeiten Nucleotide sequence Laboratory manuals Sequence Analysis, DNA Laboratory Manuals Methode DNS-Sequenz Labortechnik DNS Sequenzanalyse Chemie Aufsatzsammlung |
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| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT grahamcolina dnasequencingprotocols |
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