Neurotrophin protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Schriftenreihe: | Methods in molecular biology
169 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 274 S. Ill. |
| ISBN: | 0896036995 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV013532286 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 010116s2001 a||| |||| 00||| eng d | ||
| 020 | |a 0896036995 |9 0-89603-699-5 | ||
| 035 | |a (OCoLC)44420495 | ||
| 035 | |a (DE-599)BVBBV013532286 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-12 | ||
| 050 | 0 | |a QP552.N36 | |
| 082 | 0 | |a 573.8/4 |2 21 | |
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 245 | 1 | 0 | |a Neurotrophin protocols |c ed. by Robert A. Rush |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2001 | |
| 300 | |a X, 274 S. |b Ill. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 169 | |
| 650 | 4 | |a Sistema nervioso central - Manual de laboratorio | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Nerve Growth Factors |v Laboratory Manuals | |
| 650 | 4 | |a Nerve growth factor |v Laboratory manuals | |
| 650 | 0 | 7 | |a Immuncytochemie |0 (DE-588)4161368-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Genexpression |0 (DE-588)4020136-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Neurotropher Faktor |0 (DE-588)4317638-0 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Neurotropher Faktor |0 (DE-588)4317638-0 |D s |
| 689 | 0 | 1 | |a Immuncytochemie |0 (DE-588)4161368-5 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Neurotropher Faktor |0 (DE-588)4317638-0 |D s |
| 689 | 1 | 1 | |a Genexpression |0 (DE-588)4020136-3 |D s |
| 689 | 1 | |5 DE-604 | |
| 689 | 2 | 0 | |a Neurotropher Faktor |0 (DE-588)4317638-0 |D s |
| 689 | 2 | |5 DE-604 | |
| 700 | 1 | |a Rush, Robert A. |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 169 |w (DE-604)BV035362695 |9 169 | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009237459&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-009237459 | ||
Datensatz im Suchindex
| _version_ | 1804128326437044224 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
Part 1 Neurotrophin Quantification and Localization
1 Localization of Neurotrophin Proteins Within the Central Nervous
System by Immmunohistochemistry
James M. Conner 3
2 Neurotrophin Immunohistochemistry in Peripheral Tissues
Robert A. Rush and Xin Fu Zhou 21
3 Extraction and Quantification of the Neurotrophins
Shu Hua Zhang, Xin Fu Zhou, and Robert A. Rush 31
4 Identifying Novel Proteins in Nervous Tissue Using Microsequencing
Techniques
Selena E. Bartlett 43
Part 2 RNA Quantification and Localization
5 Quantification of mRNA Levels Using Northern Blotting
Paul Fernyhough 53
6 Quantification of mRNA Levels Using Ribonuclease Protection Assay
PirkkoHenttu 65
7 Quantification of Neurotrophin mRNA by RT PCR
Edward Chie, Dennis Liu, Xin Fu Zhou, and Robert A. Rush 81
8 Sensitive and Nonradioactive In Situ Detection of Neurotrophin
mRNAs in the Nervous System
Cory J. Xian and Xin Fu Zhou 91
Part 3 Construction of Recombinant Proteins, and Transgenic Cells and Animals
9 Expression of Neurotrophic Factors such as GDNF in the
E. coli System
Bing Ren Huang and Xue Mei Ma 101
vii
viii Contents
10 Construction of Cells Expressing Neurotrophins
Jasodhara Ray and Fred H. Gage 115
11 Efficient Generation of Stable Pheochromocytoma (PC12) Cell
Lines Using a Recombinant Retrovirus (LNC)
Matthew E. Cunningham, Jan K. Kitajewski, and
Lloyd A. Greene 135
12 Construction and Analysis of Transgenic Animals Overexpressing
Neurotrophins
Kathryn M. Albers and Brian M. Davis 149
13 Construction of Transgenic Animals Overproducing Neurotrophins
and Their Receptors
Carrie G. Causing and Freda D. Miller 167
Part 4 In Vivo Analysis
14 Tracing with Radiolabeled Neurotrophins
Christopher S. von Bartheld 195
15 Toxin Induced Death of Neurotrophin Sensitive Neurons
Ronald G. Wiley 217
16 Oligonucleotides as Inhibitors of Protein Synthesis
W. Ruwan Epa, Graham L. Barrett, and Perry F. Bartlett 223
17 The IODO GEN Method for Labeling and the Use of Retrograde
Axonal Transport to Assay Neurotrophins
Ian A. Hendry and Anna J. Reynolds 243
18 Stereological Estimation of Neuronal Number:
The Optical Disector/Cavalieri Combination
Victor Nurcombe, Nigel G. Wreford, and John F. Bertram 251
Index 269
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV013532286 |
| callnumber-first | Q - Science |
| callnumber-label | QP552 |
| callnumber-raw | QP552.N36 |
| callnumber-search | QP552.N36 |
| callnumber-sort | QP 3552 N36 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 4150 |
| ctrlnum | (OCoLC)44420495 (DE-599)BVBBV013532286 |
| dewey-full | 573.8/4 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 573 - Specific physiological systems in animals |
| dewey-raw | 573.8/4 |
| dewey-search | 573.8/4 |
| dewey-sort | 3573.8 14 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02051nam a2200529 cb4500</leader><controlfield tag="001">BV013532286</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">010116s2001 a||| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0896036995</subfield><subfield code="9">0-89603-699-5</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)44420495</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV013532286</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-12</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QP552.N36</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">573.8/4</subfield><subfield code="2">21</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Neurotrophin protocols</subfield><subfield code="c">ed. by Robert A. Rush</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2001</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">X, 274 S.</subfield><subfield code="b">Ill.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in molecular biology</subfield><subfield code="v">169</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Sistema nervioso central - Manual de laboratorio</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Wetenschappelijke technieken</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Wissenschaftliches Arbeiten</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Nerve Growth Factors</subfield><subfield code="v">Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Nerve growth factor</subfield><subfield code="v">Laboratory manuals</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Immuncytochemie</subfield><subfield code="0">(DE-588)4161368-5</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Genexpression</subfield><subfield code="0">(DE-588)4020136-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Neurotropher Faktor</subfield><subfield code="0">(DE-588)4317638-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Neurotropher Faktor</subfield><subfield code="0">(DE-588)4317638-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Immuncytochemie</subfield><subfield code="0">(DE-588)4161368-5</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Neurotropher Faktor</subfield><subfield code="0">(DE-588)4317638-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Genexpression</subfield><subfield code="0">(DE-588)4020136-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="2" ind2="0"><subfield code="a">Neurotropher Faktor</subfield><subfield code="0">(DE-588)4317638-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="2" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rush, Robert A.</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in molecular biology</subfield><subfield code="v">169</subfield><subfield code="w">(DE-604)BV035362695</subfield><subfield code="9">169</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009237459&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-009237459</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV013532286 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:47:28Z |
| institution | BVB |
| isbn | 0896036995 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009237459 |
| oclc_num | 44420495 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-12 |
| owner_facet | DE-355 DE-BY-UBR DE-12 |
| physical | X, 274 S. Ill. |
| publishDate | 2001 |
| publishDateSearch | 2001 |
| publishDateSort | 2001 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Neurotrophin protocols ed. by Robert A. Rush Totowa, NJ Humana Press 2001 X, 274 S. Ill. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 169 Sistema nervioso central - Manual de laboratorio Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Nerve Growth Factors Laboratory Manuals Nerve growth factor Laboratory manuals Immuncytochemie (DE-588)4161368-5 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Neurotropher Faktor (DE-588)4317638-0 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Neurotropher Faktor (DE-588)4317638-0 s Immuncytochemie (DE-588)4161368-5 s DE-604 Genexpression (DE-588)4020136-3 s Rush, Robert A. Sonstige oth Methods in molecular biology 169 (DE-604)BV035362695 169 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009237459&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Neurotrophin protocols Methods in molecular biology Sistema nervioso central - Manual de laboratorio Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Nerve Growth Factors Laboratory Manuals Nerve growth factor Laboratory manuals Immuncytochemie (DE-588)4161368-5 gnd Genexpression (DE-588)4020136-3 gnd Neurotropher Faktor (DE-588)4317638-0 gnd |
| subject_GND | (DE-588)4161368-5 (DE-588)4020136-3 (DE-588)4317638-0 (DE-588)4143413-4 |
| title | Neurotrophin protocols |
| title_auth | Neurotrophin protocols |
| title_exact_search | Neurotrophin protocols |
| title_full | Neurotrophin protocols ed. by Robert A. Rush |
| title_fullStr | Neurotrophin protocols ed. by Robert A. Rush |
| title_full_unstemmed | Neurotrophin protocols ed. by Robert A. Rush |
| title_short | Neurotrophin protocols |
| title_sort | neurotrophin protocols |
| topic | Sistema nervioso central - Manual de laboratorio Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Nerve Growth Factors Laboratory Manuals Nerve growth factor Laboratory manuals Immuncytochemie (DE-588)4161368-5 gnd Genexpression (DE-588)4020136-3 gnd Neurotropher Faktor (DE-588)4317638-0 gnd |
| topic_facet | Sistema nervioso central - Manual de laboratorio Wetenschappelijke technieken Wissenschaftliches Arbeiten Nerve Growth Factors Laboratory Manuals Nerve growth factor Laboratory manuals Immuncytochemie Genexpression Neurotropher Faktor Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009237459&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT rushroberta neurotrophinprotocols |