Cytoskeleton methods and protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2001
|
| Schriftenreihe: | Methods in molecular biology
161 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 288 S. graph. Darst. |
| ISBN: | 0896037711 |
Internformat
MARC
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| 245 | 1 | 0 | |a Cytoskeleton methods and protocols |c ed. by Ray H. Gavin |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2001 | |
| 300 | |a XIV, 288 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 650 | 4 | |a Cytosquelette - Manuels de laboratoire | |
| 650 | 4 | |a Protéines du cytosquelette - Manuels de laboratoire | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Cytoskeletal Proteins |v Laboratory Manuals | |
| 650 | 4 | |a Cytoskeletal proteins |v Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1804128326402441216 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I. Identification of Cytoskeleton Proteins
1 Using an Inverse PCR Strategy to Clone Large, Contiguous
Genomic DNA Fragments
Jorge A. Garc s and Ray H. Gavin 3
2 Microsequencing of Myosins for PCR Primer Design
Elaine L. Bearer 9
3 Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena
Gangadhara Sailaja, Leslie M. Lincoln, Jifan Chen,
and David J. Asai 17
Part II. Microscopy Applications
4 Video Enhanced Microscopy for Analysis of Cytoskeleton
Structure and Function
George M. Langford 31
5 Computer Assisted Systems for the Analysis of Amoeboid
Cell Motility
David R. Soil, Deborah Wessels, Edward Voss,
and Olof Johnson 45
6 Evaluation of Individual Cell Motility
Peter S. Walmod, Rasmus Hartmann Petersen,
Anton Berezin, Seren Prag, Vladislav V. Kiselyov,
Vladimir Berezin, and Elisabeth Bock 59
7 Evaluation of Cell Morphology
Eugene A. Lepekhin, Peter S. Walmod, Anton Berezin,
Vladimir Berezin, and Elisabeth Bock 85
8 A Quantitative Assay for Measurement of Chemokinesis
in Tetrahymena
Uffe Koppelhus, Per Hellung Larsen, and Vagn Leick 101
vii
Part III. Reagents for Studying Cytoskeleton Protein Function
9 Jasplakinolide: An Actin Specific Reagent that Promotes
Actin Polymerization
Andreas Holzinger 109
10 Site Directed Antibodies as Tools For Investigating Structure
and Function of Cytoskeleton Proteins
Walter Steffen and Julie L. Hodgkinson 121
11 Direct Labeling of Components in Protein Complexes
by Immuno Electron Microscopy
Julie L. Hodgkinson and Walter Steffen 133
12 Tat Mediated Delivery of Antibodies into Cultured Cells
Walter Steffen 141
Part IV. Cytoskeleton Dynamics
13 Studying Cytoskeletal Dynamics in Living Cells Using Green
Fluorescent Protein
Yisang Yoon, Kelly R. Pitts, and Mark A. McNiven 151
14 Use of Green Fluorescent Protein to Study Cellular
Dynamics: Constructing GFP Tagged Motor Enzymes
Hong Cao, Heather M. Thompson, Eugene W. Krueger,
and Mark A. McNiven 165
15 Transient Transfections and Heterokaryons as Tools
for the Analysis of Keratin IF Dynamics
Jesus M. Paramio and Jose L. Jorcano 189
Part V. Cellular Systems as Tools for Investigating Cytoskeleton
Structure and Function
16 Chromatophores as Tools for the Study of Organelle Transport
Bruce R. Telzer and Leah T. Haimo 201
17 Centriole Duplication, Centrosome Maturation, and Spindle
Assembly in Lysates of Spisula solidissima Oocytes
Andrew W. Suddith, Eugeni A. Vaisberg, Sergei A. Kuznetsov,
Walter Steffen, Conly L. Rieder, and Robert E. Palazzo 215
18 Xenopus Egg Extracts as a Model System for Analysis of Microtubule,
Actin Filament, and Intermediate Filament Interactions
Craig A. Mandato, Kari L. Weber, Anna J. Zandy,
Thomas J. Keating, and William M. Bement 229
19 Detergent Extracted Models for the Study of Cilia or Flagella
Charles B. Lindemann and Kathleen A. Schmitz 241
Part VI. Genetic Approaches for Studies of Cytoskeleton Protein Function
20 A Yeast Two Hybrid Approach for Probing Cytoskeletal
Protein Interactions
Jin jun Meng and Wallace Ip 255
21 Manipulating Dynein Genes in Tetrahymena
David J. Asai, K. Mark DeWall, Leslie M. Lincoln,
and Renotta K. Smith 269
22 Functional Analysis of Cytoskeletal Components in the Developing
Visual System of Drosophila
QiHe 279
Index 285
|
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| indexdate | 2024-07-09T18:47:28Z |
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| isbn | 0896037711 |
| language | English |
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| spelling | Cytoskeleton methods and protocols ed. by Ray H. Gavin Totowa, NJ Humana Press 2001 XIV, 288 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 161 Biologia celular larpcal Citoesqueleto larpcal Cytosquelette - Manuels de laboratoire Protéines du cytosquelette - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cytoskeletal Proteins Laboratory Manuals Cytoskeletal proteins Laboratory manuals Cytoskeleton Laboratory Manuals Cytoskeleton Laboratory manuals Mikroskopie (DE-588)4039238-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Isolierung Chemie (DE-588)4122223-4 gnd rswk-swf Zellskelett (DE-588)4121944-2 gnd rswk-swf Struktur (DE-588)4058125-1 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zellskelett (DE-588)4121944-2 s Proteine (DE-588)4076388-2 s Isolierung Chemie (DE-588)4122223-4 s DE-604 Molekulargenetik (DE-588)4039987-4 s Struktur (DE-588)4058125-1 s Mikroskopie (DE-588)4039238-7 s Methode (DE-588)4038971-6 s Gavin, Ray H. Sonstige (DE-588)139565035 oth Methods in molecular biology 161 (DE-604)BV035362695 161 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009237442&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cytoskeleton methods and protocols Methods in molecular biology Biologia celular larpcal Citoesqueleto larpcal Cytosquelette - Manuels de laboratoire Protéines du cytosquelette - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cytoskeletal Proteins Laboratory Manuals Cytoskeletal proteins Laboratory manuals Cytoskeleton Laboratory Manuals Cytoskeleton Laboratory manuals Mikroskopie (DE-588)4039238-7 gnd Methode (DE-588)4038971-6 gnd Isolierung Chemie (DE-588)4122223-4 gnd Zellskelett (DE-588)4121944-2 gnd Struktur (DE-588)4058125-1 gnd Molekulargenetik (DE-588)4039987-4 gnd Proteine (DE-588)4076388-2 gnd |
| subject_GND | (DE-588)4039238-7 (DE-588)4038971-6 (DE-588)4122223-4 (DE-588)4121944-2 (DE-588)4058125-1 (DE-588)4039987-4 (DE-588)4076388-2 (DE-588)4143413-4 |
| title | Cytoskeleton methods and protocols |
| title_auth | Cytoskeleton methods and protocols |
| title_exact_search | Cytoskeleton methods and protocols |
| title_full | Cytoskeleton methods and protocols ed. by Ray H. Gavin |
| title_fullStr | Cytoskeleton methods and protocols ed. by Ray H. Gavin |
| title_full_unstemmed | Cytoskeleton methods and protocols ed. by Ray H. Gavin |
| title_short | Cytoskeleton methods and protocols |
| title_sort | cytoskeleton methods and protocols |
| topic | Biologia celular larpcal Citoesqueleto larpcal Cytosquelette - Manuels de laboratoire Protéines du cytosquelette - Manuels de laboratoire Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cytoskeletal Proteins Laboratory Manuals Cytoskeletal proteins Laboratory manuals Cytoskeleton Laboratory Manuals Cytoskeleton Laboratory manuals Mikroskopie (DE-588)4039238-7 gnd Methode (DE-588)4038971-6 gnd Isolierung Chemie (DE-588)4122223-4 gnd Zellskelett (DE-588)4121944-2 gnd Struktur (DE-588)4058125-1 gnd Molekulargenetik (DE-588)4039987-4 gnd Proteine (DE-588)4076388-2 gnd |
| topic_facet | Biologia celular Citoesqueleto Cytosquelette - Manuels de laboratoire Protéines du cytosquelette - Manuels de laboratoire Wetenschappelijke technieken Wissenschaftliches Arbeiten Cytoskeletal Proteins Laboratory Manuals Cytoskeletal proteins Laboratory manuals Cytoskeleton Laboratory Manuals Cytoskeleton Laboratory manuals Mikroskopie Methode Isolierung Chemie Zellskelett Struktur Molekulargenetik Proteine Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009237442&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT gavinrayh cytoskeletonmethodsandprotocols |