Fixation and imaging of biological elements: heavy metals, diffusible substances, ions, peptides, and lipids
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Jena
Urban und Fischer
2000
|
| Schriftenreihe: | Progress in histochemistry and cytochemistry
35,2 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | S. 76 - 183 Ill., graph. Darst. |
Internformat
MARC
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| 100 | 1 | |a Mizuhira, Vinci |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Fixation and imaging of biological elements |b heavy metals, diffusible substances, ions, peptides, and lipids |c Vinci Mizuhira ; Hiroshi Hasegawa ; Mitsuru Notoya |
| 264 | 1 | |a Jena |b Urban und Fischer |c 2000 | |
| 300 | |a S. 76 - 183 |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 490 | 1 | |a Progress in histochemistry and cytochemistry |v 35,2 | |
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| 700 | 1 | |a Hasegawa, Hiroshi |e Verfasser |4 aut | |
| 700 | 1 | |a Notoya, Mitsuru |e Verfasser |4 aut | |
| 830 | 0 | |a Progress in histochemistry and cytochemistry |v 35,2 |w (DE-604)BV000005277 |9 35,2 | |
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Datensatz im Suchindex
| _version_ | 1804128275970129920 |
|---|---|
| adam_text | Contents
1 Introduction 9
1.1 Somatostatin 9
1.2 Somatostatin receptors 10
2 Production of antibodies 13
2.1 Construction of recombinant plasmids 13
2.2 Transformation of bacteria 14
2.3 Expression of fusion proteins 14
2.4 Immunization 15
3 Screening of antisera 17
3.1 Membrane preparations 17
3.2 Western blot 17
4 Results of western blot analysis 19
5 Localization of somatostatin receptors by immunohistochemistry .... 25
5.1 Tissues and fixation of tissues 25
5.2 Antigen retrieval 25
5.3 Immunohistochemistry at the light microscopical level by using
enzyme labelling 26
5.4 Light microscopical double immunofluorescence 26
5.5 Visualization of somatostatin receptors at the electron microscopical
level 27
6 Localization of somatostatin receptors at the light
and electron microscopical level 29
6.1 Localization of somatostatin receptors in the brain 29
6.2 Localization of somatostatin receptors in the hypothalamus
and pituitary gland 40
6.2.1 Pituitary 41
6.2.2 Hypothalamus 41
6.3 Localization of somatostatin receptors in the retina 46
7 General comments and conclusion 55
7.1 Immunocytochemical visualization of somatostatin receptors 55
7.2 Biological function of somatostatin receptors 56
References 59
|
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| id | DE-604.BV013484711 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:46:40Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009203909 |
| oclc_num | 76270154 |
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| physical | S. 76 - 183 Ill., graph. Darst. |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| publisher | Urban und Fischer |
| record_format | marc |
| series | Progress in histochemistry and cytochemistry |
| series2 | Progress in histochemistry and cytochemistry |
| spelling | Mizuhira, Vinci Verfasser aut Fixation and imaging of biological elements heavy metals, diffusible substances, ions, peptides, and lipids Vinci Mizuhira ; Hiroshi Hasegawa ; Mitsuru Notoya Jena Urban und Fischer 2000 S. 76 - 183 Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Progress in histochemistry and cytochemistry 35,2 Fixierung (DE-588)4128693-5 gnd rswk-swf Farbwiedergabe (DE-588)4153731-2 gnd rswk-swf Histochemie (DE-588)4025087-8 gnd rswk-swf Histochemie (DE-588)4025087-8 s Fixierung (DE-588)4128693-5 s DE-604 Farbwiedergabe (DE-588)4153731-2 s Hasegawa, Hiroshi Verfasser aut Notoya, Mitsuru Verfasser aut Progress in histochemistry and cytochemistry 35,2 (DE-604)BV000005277 35,2 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009203909&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mizuhira, Vinci Hasegawa, Hiroshi Notoya, Mitsuru Fixation and imaging of biological elements heavy metals, diffusible substances, ions, peptides, and lipids Progress in histochemistry and cytochemistry Fixierung (DE-588)4128693-5 gnd Farbwiedergabe (DE-588)4153731-2 gnd Histochemie (DE-588)4025087-8 gnd |
| subject_GND | (DE-588)4128693-5 (DE-588)4153731-2 (DE-588)4025087-8 |
| title | Fixation and imaging of biological elements heavy metals, diffusible substances, ions, peptides, and lipids |
| title_auth | Fixation and imaging of biological elements heavy metals, diffusible substances, ions, peptides, and lipids |
| title_exact_search | Fixation and imaging of biological elements heavy metals, diffusible substances, ions, peptides, and lipids |
| title_full | Fixation and imaging of biological elements heavy metals, diffusible substances, ions, peptides, and lipids Vinci Mizuhira ; Hiroshi Hasegawa ; Mitsuru Notoya |
| title_fullStr | Fixation and imaging of biological elements heavy metals, diffusible substances, ions, peptides, and lipids Vinci Mizuhira ; Hiroshi Hasegawa ; Mitsuru Notoya |
| title_full_unstemmed | Fixation and imaging of biological elements heavy metals, diffusible substances, ions, peptides, and lipids Vinci Mizuhira ; Hiroshi Hasegawa ; Mitsuru Notoya |
| title_short | Fixation and imaging of biological elements |
| title_sort | fixation and imaging of biological elements heavy metals diffusible substances ions peptides and lipids |
| title_sub | heavy metals, diffusible substances, ions, peptides, and lipids |
| topic | Fixierung (DE-588)4128693-5 gnd Farbwiedergabe (DE-588)4153731-2 gnd Histochemie (DE-588)4025087-8 gnd |
| topic_facet | Fixierung Farbwiedergabe Histochemie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009203909&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000005277 |
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