Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2000
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | München, Techn. Univ., Diss., 2000 |
| Beschreibung: | 73 Bl. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases |c Igor Sopov |
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Datensatz im Suchindex
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| adam_text |
CONTENTS
I. INTRODUCTION
1. BASIC MECHANISMS OF CANCER METASTASIS,_ 3
2. MATRIX-DEGRADING ENZYMES AND TUMOUR CELL INVASION_4
3. INHIBITION OF MATRIX METALLOPROTEINASES_ 6
4. BATIMASTAT - A BROAD-SPECTRUM INHIBITOR OF MATRIX
METALLOPROTEINASES_._7
5. PROFILING OF DIFFERENCES IN GENE EXPRESSION.--
.11
6. DESIGN AND AIMS _YY_--12
II. MATERIAL AND METHODS
1. ANIMAL TECHNIQUES_-------^
2. RNA ISOLATION FROM TISSUES _ -
.----21
3. DNASE TREATMENT OF TOTAL RNA_._--
4. ASSESSMENT OF YIELD, PURITY AND QUALITY OF TOTAL RNA- 17
5. CDNA PROBES SYNTHESIS FROM TOTAL RNA FOR THE ATLAS ARRAY,.
.
6. COLUMN CHROMATOGRAPHY_
^
7. HYBRIDISATION OF CDNA PROBES_---
8. SYNTHESIS OF CDNA PROBES FROM TOTAL RNA FOR THE NORTHERN ANALYSIS-21
9. NORTHERN BLOT ANALYSI
S _I-_-24
10. PHOSPHORIMAGER ANALYSIS_ -
11. V ISUAL ANALYSIS_------
III. RESULTS
1. YIELD, PURITY AND QUALITY OF RNA FOR ATLAS ARRAY TECHNIQUE
AND NORTHERN BLOT ANALYSIS,_-22
2
PREPARATION OF PROBES FOR NORTHERN BLOT ANALYSIS.: ,-21
1
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/960303081
3.
HEPATOCYTE GROWTH FACTOR (SCATTER FACTOR) BUT NOT HEPATOCYTE
GROWTH FACTOR RECEPTOR (C-MET) IS OVEREXPRESSED IN THE LIVER FROM
BATIMASTAT-TREATED MICE_
32
4. SCREENING OF DIFFERENTIAL EXPRESSION OF GENES IN THE LIVER AND LUNG
OF BATIMASTAT-TREATED MICE USING ATLAS
YY
MOUSE CDNA
EXPRESSION ARRAY-,-33
5. CORROBORATION OF DIFFERENCES IN EXPRESSION OF SOME CANDIDATE GENES
BY NORTHERN BLOT ANALYSIS_.
45
IV: DISCUSSION ~
1. IMPLICATION OF HEPATOCYTE GROWTH FACTOR, BASIC FIBROBLAST GROWTH
FACTOR,
ANGIOGENIN AND INTERLEUKIN CONVERTING ENZYME IN INCREASING THE
SUSCEPTIBILITY OF THE LIVER TO TUMOUR CELLS_ 47
2. APPLICATION AND LIMITATIONS OF THE ATLAS ARRAY TECHNIQUE _52
V. ABSTRACT_ 55
V
VI. REFERENCES .
56
VH. LIST OF FIGURES AND TABLES_68
VIII. ABBREVIATIONS_ 21
IX. ACKNOWLEDGMENTS_
11
X. CURRICULUM VITAE_ 73
2 |
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| spelling | Sopov, Igor 1973- Verfasser (DE-588)122535049 aut Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases Igor Sopov 2000 73 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Techn. Univ., Diss., 2000 Hochschulschrift gtt (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009192278&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sopov, Igor 1973- Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases Hochschulschrift gtt |
| subject_GND | (DE-588)4113937-9 |
| title | Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases |
| title_auth | Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases |
| title_exact_search | Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases |
| title_full | Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases Igor Sopov |
| title_fullStr | Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases Igor Sopov |
| title_full_unstemmed | Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases Igor Sopov |
| title_short | Investigation of gene expression in the liver of mice treated with Batimastat, a synthetic inhibitor of matrix metalloproteinases |
| title_sort | investigation of gene expression in the liver of mice treated with batimastat a synthetic inhibitor of matrix metalloproteinases |
| topic | Hochschulschrift gtt |
| topic_facet | Hochschulschrift |
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| work_keys_str_mv | AT sopovigor investigationofgeneexpressionintheliverofmicetreatedwithbatimastatasyntheticinhibitorofmatrixmetalloproteinases |