Flow cytometry: a practical approach
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Oxford [u.a.]
Oxford Univ. Pr.
2000
|
| Ausgabe: | 3. ed. |
| Schriftenreihe: | The practical approach series
229 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Hier auch später erschienene, unveränderte Nachdrucke |
| Beschreibung: | XX, 276 S. graph. Darst. |
| ISBN: | 019963825X 0199638241 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV013466953 | ||
| 003 | DE-604 | ||
| 005 | 20160114 | ||
| 007 | t | ||
| 008 | 001201s2000 d||| |||| 00||| eng d | ||
| 020 | |a 019963825X |9 0-19-963825-X | ||
| 020 | |a 0199638241 |9 0-19-963824-1 | ||
| 035 | |a (OCoLC)43063191 | ||
| 035 | |a (DE-599)BVBBV013466953 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-M49 |a DE-634 |a DE-11 |a DE-578 |a DE-703 | ||
| 050 | 0 | |a QH585.5.F56 | |
| 082 | 0 | |a 571.6/028 |2 21 | |
| 084 | |a WC 5100 |0 (DE-625)148108:13423 |2 rvk | ||
| 084 | |a WE 2000 |0 (DE-625)148264:13423 |2 rvk | ||
| 084 | |a BIO 770f |2 stub | ||
| 084 | |a QY 95 |2 nlm | ||
| 084 | |a BIO 040f |2 stub | ||
| 084 | |a BIO 655f |2 stub | ||
| 084 | |a BIO 220f |2 stub | ||
| 084 | |a BIO 200f |2 stub | ||
| 245 | 1 | 0 | |a Flow cytometry |b a practical approach |c ed. by Michael G. Ormerod |
| 250 | |a 3. ed. | ||
| 264 | 1 | |a Oxford [u.a.] |b Oxford Univ. Pr. |c 2000 | |
| 300 | |a XX, 276 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a The practical approach series |v 229 | |
| 500 | |a Hier auch später erschienene, unveränderte Nachdrucke | ||
| 650 | 4 | |a Cytométrie de flux - Guides, manuels, etc | |
| 650 | 4 | |a Flow cytometry | |
| 650 | 4 | |a Flow Cytometry |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Flow cytometry |v Handbooks, manuals, etc | |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Durchflusscytometrie |0 (DE-588)4226628-2 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Durchflusscytometrie |0 (DE-588)4226628-2 |D s |
| 689 | 0 | 1 | |a Methode |0 (DE-588)4038971-6 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Ormerod, Michael G. |e Sonstige |4 oth | |
| 830 | 0 | |a The practical approach series |v 229 |w (DE-604)BV007921321 |9 229 | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009191914&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-009191914 | ||
Datensatz im Suchindex
| _version_ | 1804128258620391424 |
|---|---|
| adam_text | Contents
Preface v
List of protocols xiii
Abbreviations xvii
1 Introduction to the principles of flow cytometry /
Ni^i l I . Curler and Michael ( ,. Ormcrod
1 Introduction 1
2 Techniques for sample preparation 2
3 Fluidics 3
4 Detection and measurement 7
5 Cell sorting 21
References 22
2 Fluorescence and fluorochromes 23
Michael G. Ormerod
1 Introduction 23
2 Principles 23
3 Fluorophores used to label covalently other probes 25
4 Fluorophores used to label directly cell components 2.S
References 33
3 Preparing suspensions of single cells is
MiifiiU i (. . Ormennl
1 Introduction 35
2 Cells from peripheral blood .IS
3 Leucocytes from other tissue 3H
4 Other types of cell 39
5 Cultured cells 44
References 45
vi
CONTENTS
4 Flow sorting 47
Nigel P. Carter and Michael G. Ormerod
1 Introduction 47
2 Principles of flow sorting 47
3 Parameters which affect sorting 50
4 Practical considerations 52
5 Specialized features 57
6 Other flow sorting systems 58
Further reading 59
References 59
5 Immunofluorescence of surface markers 61
Michael R. Loken, Cherie L Green and Denise A. Wells
1 Introduction 61
2 Instrument standardization 63
3 Testing sensitivity and resolution 68
4 Immunofluorescence 70
5 Staining for surface markers 70
6 Titration of antibodies 72
7 CD nomenclature 72
8 Population assignment 72
9 Constructing an immunophenotyping panel 73
10 Isotype controls 80
11 DNA content/surface marker analysis 81
References 82
6 Analysis of DNA—general methods 83
Michael G. Ormerod
1 Introduction 83
2 Definitions 83
3 General comments 85
4 Experimental methods 89
5 Deconvolution of the DNA histogram 93
Acknowledgement 96
References 96
7 Further clinical applications 99
Terry Hoy, Steve Garner, Brian K. Shenton, Alison E. Bell, Mark W. Lowdell, John Farrant,
Margaret North, and Carrock Sewell
1 Introduction 99
2 The enumeration of reticulocytes in peripheral blood 99
CONTENTS
3 The enumeration of CD34+ cells in peripheral blood stem cell
harvests and bone marrow harvests 101
4 Simultaneous detection of granulocyte and lymphocyte reactive
antibodies 104
5 Detection of platelet reactive antibodies 107
6 Application of crossmatching by flow cytometry to transplantation 109
7 Monitoring anti thymocyte globulin (ATG)/anti lymphocyte globulin (ALG)
therapy in transplantation 111
8 Measurement of cell mediated cytotoxicity by flow cytometry 114
9 Monitoring cell activation in response to antigens or mitogens 116
10 Measuring intracellular cytokines by flow cytometry 117
References 122
8 Quality assurance in the clinical laboratory 125
Jan W. Gratama and David Barnett
1 Introduction 125
2 Reference materials for calibration 125
3 Internal quality control procedures 127
4 External quality assurance 130
5 Conclusions 131
References 132
9 Measurement of cytoplasmic and nuclear antigens 133
Jorgen K. Larsen
1 Introduction 133
2 Methods of cell permeabilization 134
3 Methods of cell staining and analysis 142
4 Examples of intracellular antigens that can be measured by flow
cytometry 146
References 155
10 Analysis of DNA—measurement of cell kinetics by the
bromodeoxyuridine/anti bromodeoxyuridine method 159
George D. Wilson
1 Introduction 15 J
2 Basic cell kinetic concepts 160
3 Background to the BrdUrd technique 162
4 Methods of BrdUrd incorporation 164
5 Tissue preparation 166
6 Staining procedures 167
7 Flow cytometry 172
CONTENTS
8 Examples of data and analysis 172
9 Conclusions 176
Acknowledgement 177
References 177
11 Analysis of cell proliferation using the bromodeoxyuridine/
Hoechst ethidium bromide method 179
Michael G. Ormerod and Martin Poot
1 Introduction 179
2 Cell culture and BrdUrd labelling 179
3 Cell staining and flow cytometry 180
4 Synchronized cells 182
5 Asynchronous cells 183
6 Troubleshooting 186
Acknowledgement 188
References 188
12 Chromosome analysis and sorting by flow cytometry 189
Derek C. Davies, Simon P. Monard, and Bryan D. Young
1 Introduction 189
2 Preparation of chromosome suspensions 192
3 Instrumentation 196
4 Flow sorting chromosomes for library construction 197
5 Generation of chromosome paints 198
References 201
13 Intracellular ionized calcium, magnesium, membrane potential, and pH 203
Peter S. Rabinovitch and Carl H. June
1 Intracellular ionized calcium 203
2 Magnesium 222
3 Membrane potential 222
4 Measurement of cytoplasmic pH 227
Acknowledgement 232
References 232
14 Flow cytometry in the study of apoptosis 235
Michael G. Ormerod
1 Introduction 235
2 Morphological and biochemical changes 235
CONTENTS
3 Measurement of DNA degradation 236
4 Mitochondrial membrane potential 240
5 Measurements of changes in the plasma membrane 243
6 Other measurements 245
7 Quantification of apoptotic cells 246
Acknowledgement 247
References 247
15 Further applications to cell biology 249
Michael G. Ormerod
1 Introduction 249
2 Estimating cell viability 249
3 Monitoring electropermeabilization of cells 251
4 Measurement of oxidative burst 253
5 Characterizing multi drug resistance (MDR) in cancer cells 254
Al Safety procedures 259
1 Bench top flow cytometers 259
2 Cell sorters 259
3 Chemicals 260
A2 Flow cytometers and software 261
1 Commercial flow cytometers 263
2 Software for data manipulation 263
A3 List of suppliers 265
Index 273
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV013466953 |
| callnumber-first | Q - Science |
| callnumber-label | QH585 |
| callnumber-raw | QH585.5.F56 |
| callnumber-search | QH585.5.F56 |
| callnumber-sort | QH 3585.5 F56 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 5100 WE 2000 |
| classification_tum | BIO 770f BIO 040f BIO 655f BIO 220f BIO 200f |
| ctrlnum | (OCoLC)43063191 (DE-599)BVBBV013466953 |
| dewey-full | 571.6/028 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6/028 |
| dewey-search | 571.6/028 |
| dewey-sort | 3571.6 228 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| edition | 3. ed. |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02081nam a2200553 cb4500</leader><controlfield tag="001">BV013466953</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20160114 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">001201s2000 d||| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">019963825X</subfield><subfield code="9">0-19-963825-X</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0199638241</subfield><subfield code="9">0-19-963824-1</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)43063191</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV013466953</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-M49</subfield><subfield code="a">DE-634</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-578</subfield><subfield code="a">DE-703</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH585.5.F56</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">571.6/028</subfield><subfield code="2">21</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 5100</subfield><subfield code="0">(DE-625)148108:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WE 2000</subfield><subfield code="0">(DE-625)148264:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIO 770f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">QY 95</subfield><subfield code="2">nlm</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIO 040f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIO 655f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIO 220f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIO 200f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Flow cytometry</subfield><subfield code="b">a practical approach</subfield><subfield code="c">ed. by Michael G. Ormerod</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">3. ed.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Oxford [u.a.]</subfield><subfield code="b">Oxford Univ. Pr.</subfield><subfield code="c">2000</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XX, 276 S.</subfield><subfield code="b">graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">The practical approach series</subfield><subfield code="v">229</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Hier auch später erschienene, unveränderte Nachdrucke</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cytométrie de flux - Guides, manuels, etc</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Flow cytometry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Flow Cytometry</subfield><subfield code="x">methods</subfield><subfield code="v">Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Flow cytometry</subfield><subfield code="v">Handbooks, manuals, etc</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Durchflusscytometrie</subfield><subfield code="0">(DE-588)4226628-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Durchflusscytometrie</subfield><subfield code="0">(DE-588)4226628-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Methode</subfield><subfield code="0">(DE-588)4038971-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ormerod, Michael G.</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">The practical approach series</subfield><subfield code="v">229</subfield><subfield code="w">(DE-604)BV007921321</subfield><subfield code="9">229</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009191914&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-009191914</subfield></datafield></record></collection> |
| id | DE-604.BV013466953 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:46:23Z |
| institution | BVB |
| isbn | 019963825X 0199638241 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009191914 |
| oclc_num | 43063191 |
| open_access_boolean | |
| owner | DE-M49 DE-BY-TUM DE-634 DE-11 DE-578 DE-703 |
| owner_facet | DE-M49 DE-BY-TUM DE-634 DE-11 DE-578 DE-703 |
| physical | XX, 276 S. graph. Darst. |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| publisher | Oxford Univ. Pr. |
| record_format | marc |
| series | The practical approach series |
| series2 | The practical approach series |
| spelling | Flow cytometry a practical approach ed. by Michael G. Ormerod 3. ed. Oxford [u.a.] Oxford Univ. Pr. 2000 XX, 276 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier The practical approach series 229 Hier auch später erschienene, unveränderte Nachdrucke Cytométrie de flux - Guides, manuels, etc Flow cytometry Flow Cytometry methods Laboratory Manuals Flow cytometry Handbooks, manuals, etc Methode (DE-588)4038971-6 gnd rswk-swf Durchflusscytometrie (DE-588)4226628-2 gnd rswk-swf Durchflusscytometrie (DE-588)4226628-2 s Methode (DE-588)4038971-6 s DE-604 Ormerod, Michael G. Sonstige oth The practical approach series 229 (DE-604)BV007921321 229 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009191914&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Flow cytometry a practical approach The practical approach series Cytométrie de flux - Guides, manuels, etc Flow cytometry Flow Cytometry methods Laboratory Manuals Flow cytometry Handbooks, manuals, etc Methode (DE-588)4038971-6 gnd Durchflusscytometrie (DE-588)4226628-2 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4226628-2 |
| title | Flow cytometry a practical approach |
| title_auth | Flow cytometry a practical approach |
| title_exact_search | Flow cytometry a practical approach |
| title_full | Flow cytometry a practical approach ed. by Michael G. Ormerod |
| title_fullStr | Flow cytometry a practical approach ed. by Michael G. Ormerod |
| title_full_unstemmed | Flow cytometry a practical approach ed. by Michael G. Ormerod |
| title_short | Flow cytometry |
| title_sort | flow cytometry a practical approach |
| title_sub | a practical approach |
| topic | Cytométrie de flux - Guides, manuels, etc Flow cytometry Flow Cytometry methods Laboratory Manuals Flow cytometry Handbooks, manuals, etc Methode (DE-588)4038971-6 gnd Durchflusscytometrie (DE-588)4226628-2 gnd |
| topic_facet | Cytométrie de flux - Guides, manuels, etc Flow cytometry Flow Cytometry methods Laboratory Manuals Flow cytometry Handbooks, manuals, etc Methode Durchflusscytometrie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009191914&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV007921321 |
| work_keys_str_mv | AT ormerodmichaelg flowcytometryapracticalapproach |