Aquatic invertebrate cell culture:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
London ; Berlin ; Heidelberg ; New York ; Barcelona ; Hong Kong
Springer
2000
Chichester, UK Praxis Publ. |
| Schriftenreihe: | Springer praxis books in aquaculture and fisheries
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | XXV, 409 S. Ill., graph. Darst. : 25 cm |
| ISBN: | 1852336463 |
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| 245 | 1 | 0 | |a Aquatic invertebrate cell culture |c Carmel Mothersill and Brian Austin |
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Datensatz im Suchindex
| _version_ | 1804128252059451392 |
|---|---|
| adam_text | Carmel Mothersill and Brian Austin
Aquatic Invertebrate
Cell Culture
Published in association with
Springer Praxis Publishing PR
Chichester, UK
Contents
Preface xiii
1 Introduction (C Mothersill and B Austin) 1
References 4
Part I Fundamental techniques 7
2 Basic methods and media (C Mothersill, A L Mulford and B Austin) 9
2 1 Animals 9
2 2 Equipment 9
2 3 Procedures 10
231 Good aseptic technique 10
232 Hygiene of animals 10
233 Appropriate disinfectant technique for the whole animal 10
234 Appropriate dissection techniques 10
235 Need of speed for processing tissues 10
236 Which incubation conditions - with or without carbon
dioxide? 11
237 Nature of vessels — plastic or glass, coated or uncoated? 11
238 Area of culture vessels 11
239 Use of antibiotics 11
2 3 10 Explants or isolated cells? 11
2 3 11 Observation of cultures 12
2 3 12 Subcultures 12
2 3 13 Use of viability tests 12
2 3 14 Characterization of the cells 12
2 4 Culture media 13
241 Which medium 13
242 Which supplements? 13
vi Contents
243 Medium-change regime 13
2 5 Conclusions 14
2 6 References 14
3 Tissue choices for aquatic invertebrate tissue culture (A Evenden) 15
3 1 Introduction 15
311 Choosing an appropriate animal 15
312 Availability of animals 16
313 Animal suitability 17
3 2 Tissue choices 18
321 Developmental status 18
322 Methodological factors 21
323 Application choices 22
324A way forward 23
3 3 References 24
4 Characterization (M Lyons-Alcantara) 25
4 1 Introduction 25
4 2 Morphology 28
4 3 Size 29
4 4 Ultrastructure 30
441 Epithelial tissue 30
442 Fibroblasts 32
4 5 Cell-growth kinetics 34
451 Cell counting at different time intervals 34
452 Mitotic index 34
453 DNA Incorporation methods 34
454 AgNOR 35
455 Flow cytometry 35
456 Telomerase activity 36
4 6 Immunohistochemical methods 36
4 7 Enzyme activity 36
4 8 Antigenicity 37
481 Structural markers 37
482 Proliferation markers 42
4 9 Differentiation 44
4 10 Histochemistry 44
4 11 DNA Fingerprinting 45
4 12 Isoenzyme analysis 45
4 13 Chromosome content 46
4 14 DNA content 46
4 15 RNA and protein 47
4 15 1 Expression of a sponge gene as a marker for the origin of
the cells 47
Contents vii
4 16 Contamination with unicellular eukaryotes 48
4 17 References 49
Part II Experiences with aquatic invertebrates 61
5 Cell cultures from crustaceans: Shrimps, crabs and crayfish
(A L Mulford and A J Villena) 63
5 1 Introduction 63
5 2 The crustaceans 64
5 3 Previous experiences of tissue culture 67
531 Short-term cultures 68
532 Long-term cultures 69
5 4 Physiological data useful to define culture media for crustacean
cells 81
5 5 Methods 93
551 Anaesthesia and decontamination procedures 95
552 Initiation of cultures 96
553 Attachment factors 106
554 Culture media 107
555 Culture conditions 110
556 Culture identification 110
5 6 Conclusions 117
5 7 References 118
6 Interaction between crustacean ectoparasites and their hosts: A tissue-
culture perspective (D T Nolan and S C Johnson) 135
6 1 Introduction 135
6 2 Important crustacean ectoparasites 136
621 Caligid fish lice 136
622 Branchiuran fish lice 137
623 Other crustacean ectoparasites of fish 137
624 Parasite-induced stress effects and disease susceptibility 137
625 Bottlenecks in studying the host-parasite interaction in
greater depth and potential new directions 139
626 Challenges in attempting cell culture from crustacean
ectoparasitic lice 141
6 3 Cell-culture trials with Argulus japonicus 144
631 Experimental animals and handling 144
632 Media composition and supplements 144
633 Tissue preparation and culture conditions 145
634 Evaluation of culture results 148
635 Characterization requirements for cells 148
6 4 Application of cell culture for control of these and other types of
parasites 153
viii Contents
6 5 Conclusions 154
6 6 Acknowledgements 155
6 7 References 155
7 Culture of molluscan cells (M F Mulcahy) 165
7 1 Introduction 165
711 Target species 165
712 Target cells 166
713 Depuration, decontamination and disinfection 169
714 Cell-isolation protocols 170
715 Cell-culture conditions 171
716 Methods to measure the functional activity of cells 173
717 Characterization of cultured cells 173
718 Approaches to the development of continuous cell lines 174
719 Cryopreservation 175
7 2 Conclusion 176
7 3 References 176
8 Molluscan diseases: A tissue-culture perspective (R A Elston) 183
8 1 Introduction 183
8 2 Viral diseases and isolation of viruses from molluscs 184
821 Viral diseases of molluscs 184
822 Culture of molluscan viruses 186
823 Dissemination of neoplasia of molluscs: A viral aetiology? , 189
8 3 Intracellular bacterial diseases of molluscs 192
8 4 Intracellular protozoan diseases of molluscs 194
8 5 Efforts to establish molluscan cell and tissue cultures 194
851 Primary culture of molluscan cells and tissues 194
852 Establishment of the Bge cell line 196
8 6 Need for the establishment of molluscan cell and tissue cultures 197
8 7 References 198
9 Primary cell culture from a sponge: Primmorphs
(W E G Muller and M R Custodio) 205
9 1 Introduction 205
9 2 Establishment of primmorphs 206
921 Sponge 206
922 Media 206
923 Dissociation of cells and formation of primmorphs 207
924 Histology 209
925 Subcultures of primmorphs 210
926 Long-term cultivation of primmorphs in the aquarium 210
9 3 Characterization of primmorphs 211
931 Level of telomerase activity in cells depending on the
culture conditions 211
Contents ix
932 Immunocytochemical detection of BrdU incorporation in
cells of primmorphs 211
933 Presence of bacteria in the primmorphs 212
934 Expression of a sponge gene as a marker for the origin of
the cells 212
9 4 Application of primmorphs 213
941 Bioindicator 213
942 Production of bioactive compounds 214
9 5 Cultivation of primmorphs under pressure 214
951 Effect of ethylene 214
952 Cloning of ethylene-responsive proteins from Suberites
domuncula 216
953 Levels of expression of sponge ethylene-responsive protein 216
9 6 Discussion of the system 218
9 7 Acknowledgements 219
9 8 Disclosure statement 219
9 9 References 220
10 Urochordate cell cultures: From in vivo to in vitro approaches
{B Rinkevich and C Rabinowitz) 225
10 1 Tunicates: importance for cell-culture applications 225
10 2 Four major tissue sources for in vitro studies 226
10 2 1 Naturally occurring cell suspensions 226
10 2 2 Organ cultures 227
10 2 3 Dissociated tissues/organs 229
10 2 4 Embryo-derived cell cultures 229
10 3 Establishment of cell cultures 230
10 4 Botryllid ascidians: Cell cultures; recent advances, old problems 231
10 4 1 Blood cells 231
10 4 2 Ampullae regeneration 232
10 4 3 Epithelial cell cultures 233
10 5 In vitro applications 234
10 5 1 Applications for environmental studies 236
10 6 Closing remarks 238
10 7 Acknowledgements 240
10 8 References 240
11 Echinodermata (C Moss, C D Bavington and J D McKenzie) 245
11 1 The echinoderms 245
11 2 Echinoderm cells and tissues 246
11 2 1 Tissue layout and cell culture 246
11 2 2 Reproductive and other proliferative tissues 249
11 3 Studies on echinoderm cells in vitro and the development of cell-
culture techniques 251
x Contents
11 3 1 The development of methodology towards long-term
echinoderm cultures 254
11 4 Applications for echinoderm cell cultures 262
11 4 1 Production of metabolites 263
11 4 2 Use of symbionts 263
11 4 3 Models for aquaculture and environmental monitoring 263
11 4 4 Pest species 264
11 5 Summary 264
11 6 References 264
Part III Uses for cell cultures 269
12 Application of cell culture for coral-reef ecology
(Z Billinghurst, MSA Ammar and WEG Mutter) 271
12 1 Introduction 271
12 1 1 Cnidarians 271
12 2 Cell culture 274
12 2 1 Primmorph system 275
12 3 Applications of the primmorph system 277
12 3 1 Diseases in corals 278
12 3 2 Coral bleaching 279
12 3 3 Molecular probes for detecting environmental stress 282
12 3 4 Case study: Monitoring the effect of light using molecular
probes 283
12 3 5 Primmorphs from Dendronephthya klunzingeri as a model
to determine the effect of UVB and visible light 284
12 4 Acknowledgements 287
12 5 Disclosure statement 287
12 6 References 287
13 Culture of bryozoans and barnacles: Application of larval cell culture to
biofouling studies (K Okano and E Hunter) 293
13 1 Introduction 293
13 2 Cells from Bryozoan larvae 295
13 2 1 Overview 295
13 2 2 Techniques and methodology 298
13 2 3 Bryozoan larval ciliated epithelium 302
13 2 4 Internal cells from bryozoan larvae 303
13 2 5 Modulation of ciliary behaviour in identified ciliated cells 305
13 3 Cells from barnacle (cirripedia) larvae 306
13 3 1 Overview 306
13 3 2 Techniques and methodology 309
13 3 3 Physiological characterization of cement secretion using
isolated organs and cultured cells 311
Contents xi
13 4 Future applications 313
13 4 1 Application of larval cell-culture techniques in biofouling
studies 313
13 4 2 Larval cells as cement factories 314
13 4 3 Larval cells and Pharmaceuticals 315
13 5 Conclusion 316
13 6 Acknowledgements 316
13 7 References 316
14 Development of sponge cell cultures for biomedical application
(S A Pompom and R Willoughby) 323
14 1 Importance of sponge cell culture 323
14 2 Sponge cell cultures 324
14 2 1 Cell culture versus tissue culture 324
14 3 Establishment of cell cultures 325
14 3 1 Selection of appropriate species for in vitro research 325
14 3 2 Identification of target cell types 326
14 3 3 Selection of appropriate methods for cell dissociation 326
14 3 4 Cryopreservation: pros and cons 327
14 4 Development of defined culture media 327
14 4 1 Basal media 329
14 4 2 Antibiotics 329
14 4 3 Growth factors 329
14 5 Selection of culture vessel 330
14 5 1 Attached versus suspension cultures 330
14 6 Cell-type verification and assessment of functional state 332
14 6 1 Chemical analyses of cultured cells 333
14 6 2 Biosynthesis of radiolabelled precursors 333
14 7 Current status and future directions 333
14 8 Acknowledgements 334
14 9 References 334
15 Applications of invertebrate cell culture in studies of biomarkers and
ecotoxicology (D Sheehan) 337
15 1 Introduction 337
15 1 1 Chemical threat posed by xenobiotics 337
15 1 2 Biological xenobiotic defence mechanisms 338
15 1 3 Enzymatic detoxification 339
15 2 Detoxification systems of invertebrates as bioindicators of
environmental pollution 342
15 2 1 Invertebrates as sentinel species 342
15 2 2 Potential of invertebrate species for in vitro toxicology 343
15 3 Primary cell cultures 343
15 4 Uses of cell lines in toxicology 347
xii Contents
15 4 1 Cytotoxicity 347
15 4 2 Ultrastructural effects 348
15 4 3 Formation of protein adducts 348
15 4 4 Genotoxicity 349
15 5 Non-mammalian tissue culture 350
15 6 Potential of invertebrate tissue culture in toxicology 351
15 7 Future perspectives 351
15 8 References 352
16 The growth and pathogenicity of bacterial and viral pathogens in aquatic
invertebrate tissue cultures (B Austin) 361
16 1 Introduction 361
16 1 1 The availability of cell cultures 362
16 1 2 The growth of viral pathogens 362
16 1 3 The growth of bacterial pathogens 363
16 1 4 Parasites 364
16 1 5 Measuring cellular damage 364
16 2 Conclusion 365
16 3 References 365
17 Validation of in vitro techniques (B Quinn and C Mothersill) 369
17 1 In vitro techniques 369
17 1 1 Validation 370
17 1 2 Validation by in vitro/in vivo comparison 373
17 1 3 Legislation and regulations 374
17 2 Tier system for use of validating in vitro techniques 374
17 2 1 QSAR 375
17 2 2 In vitro exposure 376
17 2 3 In vivo exposure 376
17 2 4 In vitro/in vivo validation 377
17 3 Validated jn vitro systems 379
17 3 1 Mutagenicity 379
17 3 2 Skin and eye irritancy 379
17 4 Toxicity studies 380
17 5 Pathogenicity studies 382
17 6 Validation by information systems and databases 383
17 6 1 Databases 384
17 6 2 The MEIC programme 385
17 7 Conclusion 385
17 8 References 387
18 Conclusions (C Mothersill and B Austin) 397
Index 401
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| id | DE-604.BV013460328 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:46:17Z |
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| isbn | 1852336463 |
| language | English |
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| physical | XXV, 409 S. Ill., graph. Darst. : 25 cm |
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| record_format | marc |
| series2 | Springer praxis books in aquaculture and fisheries |
| spelling | Aquatic invertebrate cell culture Carmel Mothersill and Brian Austin London ; Berlin ; Heidelberg ; New York ; Barcelona ; Hong Kong Springer 2000 Chichester, UK Praxis Publ. XXV, 409 S. Ill., graph. Darst. : 25 cm txt rdacontent n rdamedia nc rdacarrier Springer praxis books in aquaculture and fisheries Literaturangaben Cellules - Culture Invertébrés aquatiques - Cultures et milieux de culture Aquatic invertebrates Cultures and culture media Cell culture Wirbellose (DE-588)4066359-0 gnd rswk-swf Wassertiere (DE-588)4064802-3 gnd rswk-swf Zellkultur (DE-588)4067547-6 gnd rswk-swf Wirbellose (DE-588)4066359-0 s Wassertiere (DE-588)4064802-3 s Zellkultur (DE-588)4067547-6 s DE-604 Mothersill, Carmel 1950- Sonstige (DE-588)122047540 oth Austin, Brian 1951- Sonstige (DE-588)120623366 oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009187593&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Aquatic invertebrate cell culture Cellules - Culture Invertébrés aquatiques - Cultures et milieux de culture Aquatic invertebrates Cultures and culture media Cell culture Wirbellose (DE-588)4066359-0 gnd Wassertiere (DE-588)4064802-3 gnd Zellkultur (DE-588)4067547-6 gnd |
| subject_GND | (DE-588)4066359-0 (DE-588)4064802-3 (DE-588)4067547-6 |
| title | Aquatic invertebrate cell culture |
| title_auth | Aquatic invertebrate cell culture |
| title_exact_search | Aquatic invertebrate cell culture |
| title_full | Aquatic invertebrate cell culture Carmel Mothersill and Brian Austin |
| title_fullStr | Aquatic invertebrate cell culture Carmel Mothersill and Brian Austin |
| title_full_unstemmed | Aquatic invertebrate cell culture Carmel Mothersill and Brian Austin |
| title_short | Aquatic invertebrate cell culture |
| title_sort | aquatic invertebrate cell culture |
| topic | Cellules - Culture Invertébrés aquatiques - Cultures et milieux de culture Aquatic invertebrates Cultures and culture media Cell culture Wirbellose (DE-588)4066359-0 gnd Wassertiere (DE-588)4064802-3 gnd Zellkultur (DE-588)4067547-6 gnd |
| topic_facet | Cellules - Culture Invertébrés aquatiques - Cultures et milieux de culture Aquatic invertebrates Cultures and culture media Cell culture Wirbellose Wassertiere Zellkultur |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009187593&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mothersillcarmel aquaticinvertebratecellculture AT austinbrian aquaticinvertebratecellculture |