Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2000
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Erlangen-Nürnberg, Univ., Diss., 2000. - Mit Zsfassung in dt. Sprache |
| Beschreibung: | 153 S. Ill., graph. Darst. |
Internformat
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| 100 | 1 | |a Oustinova, Iana |d 1964- |e Verfasser |0 (DE-588)122452984 |4 aut | |
| 245 | 1 | 0 | |a Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences |c von Iana Oustinova |
| 264 | 1 | |c 2000 | |
| 300 | |a 153 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Erlangen-Nürnberg, Univ., Diss., 2000. - Mit Zsfassung in dt. Sprache | ||
| 502 | |a Erlangen, Nürnberg, Univ., Diss., 2000 | ||
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Datensatz im Suchindex
| _version_ | 1807229320051032064 |
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| adam_text |
SUBJECT
INDEX
1
SUBJECT
INDEX
_
PAGE
SUBJECT INDEX
1
LIST
OF
FIGURES
5
LIST
OF
TABLES
7
LIST
OF
ABBREVIATIONS
9
I.
INTRODUCTION
13
1.1.
SYSTEMATICS
OF
THE
ANKISTRODESMACEAE
13
1.2.
SYSTEMATICS
OF
SELECTED
GENERA
RELATED
TO
THIS
STUDY
15
1.3.
ULTRASTRUCTURAL,
CHEMOTAXONOMICAL,
AND
PHYLOGENETIC
STUDIES
OF
THE
16
ANKISTRODESTNACEAE
1.4.
SYSTEMATICS
OF
AMOEBIDIUM
PARASITICUM
CIENK.
17
1.5.
GROUP
I
INTRONS
IN
GREEN
ALGAE
18
1.6.
HOMING
ENDONUCLEASES
ENCODED
IN
GROUP
I
INTRONS
23
1.6.1.
THE
LAGLI-DADG
FAMILY
OF
HOMING
ENDONUCLEASES
23
1.6.2.
THE
HIS-CYS
BOX
FAMILY
OF
NUCLEAR
HOMING
ENDONUCLEASES
25
1.6.3.
ORIGIN
OF
HOMING
ENDONUCLEASES
27
1.7.
THE
PURPOSE
OF
THIS
WORK
28
II
MATERIALS
AND
METHODS
29
N.L.
PREPARATION
AND
ANALYSES
OF
DNA
29
N.1.1.
CULTURE
CONDITIONS
29
N.L.
2.
ISOLATION
OF
TOTAL DNA
29
II.
1.3.
AMPLIFICATION
OF
DNA
FRAGMENTS
BY
PCR
29
N.
1.4.
PRIMERS
USED
FOR
PCR,
SEQUENCING
AND
CLONING
OF
PCR
PRODUCTS
30
N.1.5.
AGAROSE
GEL
ELECTROPHORESIS
32
II.
1.6.
CLEANUP
AND
GEL
EXTRACTION
OF
PCR
PRODUCTS
33
N.L.
7.
DNA
SEQUENCING
34
U.
1.7.1
VISUALIZATION
OF
PRODUCTS
OF
SEQUENCING
REACTIONS
ON
POLYACRYLAMIDE
GELS
34
N.1.7.1.1.
PREPARATION
OF
GRADIENT
PAA
GELS
34
U.
1.7.1.2.
PAA
GEL
ELECTROPHORESIS
AND
AUTORADIOGRAPHY
34
H.1.7.2.
AUTOMATIC
DNA
SEQUENCING
35
N.L.
7.2.1.
CYCLE-SEQUENCING
REACTIONS
35
N.1.7.2.2.
PURIFICATION
OF
CYCLE-SEQUENCING
REACTION
PRODUCTS
AND
THEIR
PREPARATION
35
FOR
SEQUENCING
N.1.8.
MOLECULAR
CLONING
36
N.L.
8.1.
VECTORS
AND
E.
COLI
STRAINS
USED
FOR
CLONING
36
2
SUBJECT
INDEX
N.
1.8.2.
CLONING
INTO
THE
PGEM-T
EASY
VECTOR
36
N.1.8.3.
CLONING
INTO
THE
PUC18
SMA
I/BAP
VECTOR
36
N.I.8.4.
MOLECULAR
CLONING
OF
THE
CONSTRUCTS
USED
IN
THIS
STUDY
37
N.I.9.
DIRECT
PCR
38
N.1.10.
ISOLATION
OF
RECOMBINANT
PLASMID
DNA
38
N.1.10.1.
MINI-PREPARATIONS
OF
PLASMID
DNA
USING
THE
QIAPREP
SPIN
MINIPREP
KIT
39
N.
1.10.2.
SETP
MINI-PREPARATIONS
OF
PLASMID
DNA
(WITH
ALKALINE
LYSIS
OF
BACTERIA)
39
N.
1.10.3.
STET
MINI-PREPARATIONS
OF
PLASMID
DNA
39
N.I.N.
RESTRICTION
DIGEST
OF
PLASMID
DNA
39
N.I.12.
RADIOACTIVE
LABELING
OF
DNA
40
N.I.I3.
PREPARATION
OF
DNA
FOR
IN
VITRO
RNA
SYNTHESIS
40
N.2.
PREPARATION
AND
ANALYSES
OF
RNA
40
N.2.I.
IN
VITRO
RNA
TRANSCRIPTION
40
N.2.I.I.
TRANSCRIPTION
WITH
T7
RNA
POLYMERASE
41
N.2.
1.2.
VISUALIZING
OF
TRANSCRIBED
RNA
ON
PAA
GELS
41
N.2.2.
THE
MTESSU
2
INTRON
SELF-SPLICING
EXPERIMENTS
41
N.2.2.1.
VARIATIONS
OF
THE
SELF-SPLICING
REACTION
CONDITIONS
42
N.2.3.
ISOLATION
OF
TOTAL
RNA
42
N.2.4.
ISOLATION
OF
THE
MRNA
FRACTION
43
N.2.5.
RT-PCR
43
N.2.6.
NORTHERN
BLOT
HYBRIDIZATION
44
N.2.6.I.
DENATURING
ELECTROPHORESIS
OF
RNA
44
N.2.6.2.
BLOTTING
44
N.2.6.3.
PROBES
AND
POSITIVE
CONTROLS
FOR
NORTHERN
HYBRIDIZATION
44
N.2.6.4.
PREHYBRIDIZATION
AND
HYBRIDIZATION
45
N.3.
RNA
IN
VITRO
TRANSLATION
AND
ENDONUCLEASE
ASSAY
45
N.3.I.
PURIFICATION
OF
IN
VITRO
SYNTHESIZED
RNA
45
N.3.2.
IN
VITRO
TRANSLATION
OF
IN
VITRO
SYNTHESIZED
RNA
46
N.3.3.
SDS-PAGE
46
N.3.4.
ENDONUCLEASE
ASSAY
46
N.3.5.
SOUTHERN
BLOT
47
N.4.
PHYLOGENETIC
ANALYSES
47
N.4.I
SEQUENCE
DATA
ALIGNMENT
47
N.4.2.
CONSTRUCTION
OF
SECONDARY
STRUCTURE
MODELS
48
N.4.3.
WEIGHTING
MASKS
48
N.4.4.
PHYLOGENETIC
ANALYSES
WITH
THE
PHYLIP
AND
PAUP
PROGRAM
PACKAGES
48
N.5.
CELL
WALL
ANALYSIS
OF
HYALORAPHIDIUM
CURVATUM
49
N.6.
TRANSMISSION
ELECTRON
MICROSCOPY
OF
HYALORAPHIDIUM
CURVATUM
49
SUBJECT
INDEX
3
B
0
0
0
0
0
0
0
0
0
0
0
0
0
0
RESULTS
51
1
PHYLOGENY
OF
THE
ANKISTRODESMACEAE
BASED
ON
18S
RRNA
DATA
51
2
PRIMARY STRUCTURE
OF
THE
NUCLEAR
AND
CHLOROPLAST
SSU
RRNA
OF
68
CLOSTERIOPSIS
ACICULARIS
3
THE
PHYLOGENETIC
POSITION
OF
CLOSTERIOPSIS
ACICULARIS
BASED
ON
SSU
68
RRNA
DATA
4
18S
RRNA
PHYLOGENY
OF
HYALORAPHIDIUM
CURVATUM
71
5
TRANSMISSION
ELECTRON
MICROSCOPY
OF
H.
CURVATUM
74
6
CELL
WALL
SUGAR
COMPOSITION
OF
H.
CURVATUM
76
7
THE
PHYLOGENETIC
POSITION
OF
AMOEBIDIUM
PARASITICUM
76
8
GROUP I
INTRONS
IN
NUCLEAR
SSU
RRNA
GENES
OF
THE
ANKISTRODESMACEAE
79
9
GROUP I
INTRONS
IN
CHLOROPLAST
SSU
RRNA
GENES
OF
CLOSTERIOPSIS
94
ACICULARIS
AND
SCENEDESMUS
VACUOLATUS
10
THE
GROUP
I
INTRON
IN
CHLOROPLAST
SSU
RDNA
OF
CLOSTERIOPSIS
ACICULARIS
94
CONTAINS
AN
INSERTION
RELATED
TO
THE
LAGLI-DADG
FAMILY
OF
HOMING
ENDONUCLEASES
11
A
PUTATIVE
HOMING
ENDONUCLEASE
L-MTEL
IS
ENCODED
IN
THE
SECOND
18S
97
RDNA
GROUP
I
INTRON
OF
MONORAPHIDIUM
TERRESTRE
12
PRODUCTS
OF
MTESSU
2
SPLICING
IN
VIVO
STUDIED
BY
NORTHERN
HYBRIDIZATION
101
13
IN
VITRO
SELF-SPLICING
OF
MTESSU
2
104
14
IDENTIFICATION
OF
MRNA
FOR
A
PUTATIVE
L-MTEL
HOMING
ENDONUCLEASE
BY
105
RT-PCR
HI.15
PRELIMINARY
REPORT
ON
L-MTEL
IN
VITRO
TRANSLATION
AND
ENDONUCLEASE
ASSAY
110
IV.
DISCUSSION
115
IV.
1
1
8S
RRNA
PHYLOGENY
VERSUS
MORPHOLOGY-BASED
SYSTEMATICS
OF
THE
115
ANKISTRODESMACEAE
IV.2
CLOSTERIOPSIS
ACICULARIS
IS
CLOSELY
RELATED
TO
THE
GENUS
CHLORELLA
117
(T
REBOUXIOPHYCEAE)
IV.3
HYALORAPHIDIUM
CURVATUM
IS
NOT
A
GREEN
ALGA,
BUT
A
FUNGUS;
AMOEBIDIUM
119
PARASITICUM
IS
NOT
A
FUNGUS,
BUT
A
PROTISTAN
ANIMAL
TV
A
THE
DISTRIBUTION
OF
GROUP
I
INTRONS
IN
NUCLEAR
SSU
RRNA
GENES
OF
123
ANKISTRODESMACEAE
MAY
BE
EXPLAINED
BY
THEIR
VERTICAL
INHERITANCE,
SUBSEQUENT
LOSS,
AND
HORIZONTAL
TRANSFER
TO
HETEROLOGOUS
SITES
MEDIATED
BY
A
NUCLEAR
HOMING
ENDONUCLEASE
IV.5
THE
PRESENCE
OF
GROUP
I
INTRONS
IN
CHLOROPLAST
16S
RRNA
GENES
OF
TWO
125
GREEN
ALGAE
MAY
BE
EXPLAINED
BY
THEIR
HORIZONTAL
TRANSFER
V.
SUMMARY
127
SUBJECT
INDEX
V.
ZUSMMENFASSUNG
129
ACKNOWLEDGEMENTS
131
REFERENCES
AND
SUBMITTED
PAPERS
133
REFERENCES
133
SUBMITTED
PAPERS
148
APPENDIX
LIST
OF
MEDIA
AND
SOLUTIONS
149
CULTURE
MEDIA
149
SOLUTIONS
FOR
MOLECULAR
BIOLOGY
150
SOLUTIONS
FOR
TRANSMISSION
ELECTRON
MICROSCOPY
153 |
| any_adam_object | 1 |
| author | Oustinova, Iana 1964- |
| author_GND | (DE-588)122452984 |
| author_facet | Oustinova, Iana 1964- |
| author_role | aut |
| author_sort | Oustinova, Iana 1964- |
| author_variant | i o io |
| building | Verbundindex |
| bvnumber | BV013394748 |
| classification_rvk | WL 2830 |
| ctrlnum | (OCoLC)48720258 (DE-599)BVBBV013394748 |
| discipline | Biologie |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV013394748 |
| illustrated | Illustrated |
| indexdate | 2024-08-13T00:16:24Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009138007 |
| oclc_num | 48720258 |
| open_access_boolean | |
| owner | DE-29 DE-29T DE-355 DE-BY-UBR DE-12 DE-83 DE-188 |
| owner_facet | DE-29 DE-29T DE-355 DE-BY-UBR DE-12 DE-83 DE-188 |
| physical | 153 S. Ill., graph. Darst. |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| record_format | marc |
| spelling | Oustinova, Iana 1964- Verfasser (DE-588)122452984 aut Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences von Iana Oustinova 2000 153 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Erlangen-Nürnberg, Univ., Diss., 2000. - Mit Zsfassung in dt. Sprache Erlangen, Nürnberg, Univ., Diss., 2000 Hochschulschrift gtt Sequenzanalyse Chemie (DE-588)4132277-0 gnd rswk-swf Ankistrodesmaceae (DE-588)4613130-9 gnd rswk-swf Phylogenetik (DE-588)4174591-7 gnd rswk-swf Phylogenetische Systematik (DE-588)4174592-9 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Ankistrodesmaceae (DE-588)4613130-9 s Phylogenetische Systematik (DE-588)4174592-9 s Molekularbiologie (DE-588)4039983-7 s DE-604 Phylogenetik (DE-588)4174591-7 s Sequenzanalyse Chemie (DE-588)4132277-0 s DE-188 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009138007&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Oustinova, Iana 1964- Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences Hochschulschrift gtt Sequenzanalyse Chemie (DE-588)4132277-0 gnd Ankistrodesmaceae (DE-588)4613130-9 gnd Phylogenetik (DE-588)4174591-7 gnd Phylogenetische Systematik (DE-588)4174592-9 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4132277-0 (DE-588)4613130-9 (DE-588)4174591-7 (DE-588)4174592-9 (DE-588)4039983-7 (DE-588)4113937-9 |
| title | Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences |
| title_auth | Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences |
| title_exact_search | Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences |
| title_full | Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences von Iana Oustinova |
| title_fullStr | Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences von Iana Oustinova |
| title_full_unstemmed | Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences von Iana Oustinova |
| title_short | Molecular phylogeny of the family Ankistrodesmaceae (Chlorophyta, Chlorophyceae) and analyses of their group I intervening sequences |
| title_sort | molecular phylogeny of the family ankistrodesmaceae chlorophyta chlorophyceae and analyses of their group i intervening sequences |
| topic | Hochschulschrift gtt Sequenzanalyse Chemie (DE-588)4132277-0 gnd Ankistrodesmaceae (DE-588)4613130-9 gnd Phylogenetik (DE-588)4174591-7 gnd Phylogenetische Systematik (DE-588)4174592-9 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Hochschulschrift Sequenzanalyse Chemie Ankistrodesmaceae Phylogenetik Phylogenetische Systematik Molekularbiologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009138007&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT oustinovaiana molecularphylogenyofthefamilyankistrodesmaceaechlorophytachlorophyceaeandanalysesoftheirgroupiinterveningsequences |