The phosphorylation induced activating mechanism of the response regulator FixJ:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2000
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 153 S. Ill., graph. Darst. : 21 cm |
Internformat
MARC
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| 100 | 1 | |a Schumacher, Jörg |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a The phosphorylation induced activating mechanism of the response regulator FixJ |c vorgelegt von Jörg Schumacher |
| 264 | 1 | |c 2000 | |
| 300 | |a 153 S. |b Ill., graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Köln, Univ., Diss., 1999 | ||
| 650 | 0 | 7 | |a Genregulation |0 (DE-588)4122166-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Rhizobium meliloti |0 (DE-588)4178030-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Stickstofffixierung |0 (DE-588)4183273-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Phosphorylierung |0 (DE-588)4174459-7 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Rhizobium meliloti |0 (DE-588)4178030-9 |D s |
| 689 | 0 | 1 | |a Stickstofffixierung |0 (DE-588)4183273-5 |D s |
| 689 | 0 | 2 | |a Genregulation |0 (DE-588)4122166-7 |D s |
| 689 | 0 | 3 | |a Phosphorylierung |0 (DE-588)4174459-7 |D s |
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| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009119137&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009119137 | |
Datensatz im Suchindex
| _version_ | 1807322906698448896 |
|---|---|
| adam_text |
CONTENTS
ZUSAMMENFASSUNG
I
INTRODUCTION
1
1)
SYMBIOTIC
NITROGEN-FIXATION
3
1.1)
NITROGEN
FIXATION
3
1.2)
NITROGENASE
5
1.3)
NODULE
ORGANOGENESIS
AND
SYMBIOTIC
NITROGEN
FIXATION
9
1.4)
THE
NIF
AND
FIX
GENES
OF
SINORHIZOBIUM
MELILOTI
13
1.5)
REGULATION
OF
NIF
AND
FIX
GENES
OF
S.
MELILOTI
15
2)
TWO-COMPONENT
SYSTEMS
17
2.1)
THE
FIXU
TWO-COMPONENT
SYSTEM
23
2.2)
SEQUENCE
AND
STRUCTURE
CONSERVATION
BETWEEN
RECEIVER
DOMAINS
27
2.3)
THE
MOLECULAR
MECHANISM
OF
RESPONSE-REGULATORS
31
2.4)
PROJECT
OUTLINE
AND
OBJECTIVES
35
II
MATERIALS
AND
METHODS
39
1)
BACTERIAL
STRAINS
AND
PLASMIDS
41
2)
GENERAL TECHNIQUES
43
3)
CONSTRUCTION
OF
PJS2
43
4)
SITE DIRECTED
MUTAGENESIS
43
5)
CONSTRUCTION
OF
PJG
PLASMIDS
45
6)
PROTEIN
PURIFICATION
45
7)
P-GALACTOSIDASE
ASSAY
47
8)
ANALYTICAL
GEL
PERMEATION
CHROMATOGRAPHY
49
9)
PHOSPHORYLATION
ASSAYS
WITH
ACETYL-PHOSPHATE
49
10)
PHOSPHORYLATION
ASSAYS
BY
CAPILLARY
ISO-ELECTRIC-FOCUSSING
(IEF)
51
11)
BAND SHIFT
ASSAYS
53
12)
PHOSPHORYLATION
ASSAYS
WITH
FIXL*,
Y-[32P]ATP
AND
FIXJ
53
13)
COMPUTER
MODELLING
AND
PROTEIN
STRUCTURE
VISUALISATION
55
UI
RESULTS
AND
DISCUSSION
57
1)
PHOSPHORYLATION
INDUCED
DIMERISATION
OF
FIXJ
61
1.1)
STOICHIOMETRY
OF
PHOSPHORYLATION
61
1.2)
EVIDENCE
FOR
A
SECOND
PHOSPHORYLATION
EVENT
65
1.3)
PHOSPHORYLATION
OF
FIXJ
AND
FIXJN
ARE
SECOND
ORDER
REACTIONS
67
1.4)
PHOSPHORYLATED
FIXJ
IS
MONOMERIC
AT
LOW
CONCENTRATIONS
69
1.5)
CONCLUSION
:
MUTUAL
INHIBITION
OF
THE
FIXJ
DOMAINS
71
2)
ALANINE
SCANNING
MUTAGENESIS
OF
THE
FIXJ
RECEIVER
DOMAIN
73
2.1)
SOLVENT
EXPOSED
RESIDUES
IN
THE
N-TERMINAL
DOMAIN
OF
FIXJ
73
2.2)
COMPUTER
MODELLING
OF
THE
FIXJN
AND
FIXJ
3-D
STRUCTURE
77
2.3)
SITE-DIRECTED
MUTAGENESIS
83
2.4)
PURIFICATION
OF
FIXJ
VIA
GST-FUSION
PROTEINS
85
3)
THE
DIMERISATION
INTERFACE
OF
FIXJ
87
3.1)
THE
DIMERISATION
INTERFACE
AT
A-HELIX
4
87
3.2)
THE
DIMERISARTION
INTERFACE
DETERMINED
BY
THE
CRYSTAL
STRUCTURE
OF
PHOSPHORYLATED
FIXJN
91
3.3)
DIMERISATION
INCREASES
THE
AFFINITY
TO
THE
FIXK
PROMOTER
BUT
IS
NOT
ESSENTIAL
FOR
TRANSCRIPTIONAL
ACTIVATION
93
3.4)
DIMERISATION
STABILISES
PHOSPHORYLATED
FIXJ
95
3.5)
CONCLUSION
:
THE
ROLE
OF
FIXJ~P
DIMERISATION
97
4)
INTRAMOLECULAR
SIGNAL
TRANSDUCTION
WITHIN
FIXJ
99
4.
L)M
VIVO
ACTIVITIES
OF
MUTANT
FIXJ
101
4.2)
EVIDENCE
FOR
A
SIGNALLING
SURFACE
AT
A-HELIX4
AND
P-STRAND
5
105
4.3)
PHOSPHORYLATION
INDUCED
CONFORMATIONAL
CHANGES
IN
FIXJN
AND
THE
ROLE
OF
F101
113
5)
PHOSPHORYLATION
OF
FIXJ
BY
ITS
COGNATE
KINASE
FIXL
117
IV
CONCLUSIONS
121
REFERENCES
135
APPENDICES
153
LIST
OF
FIGURES
AND
TABLES
LIST
OF
ABBREVIATIONS
ACKNOLEDGEMENTS |
| any_adam_object | 1 |
| author | Schumacher, Jörg |
| author_facet | Schumacher, Jörg |
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| bvnumber | BV013369930 |
| ctrlnum | (OCoLC)52422015 (DE-599)BVBBV013369930 |
| format | Thesis Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV013369930 |
| illustrated | Illustrated |
| indexdate | 2024-08-14T01:03:56Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009119137 |
| oclc_num | 52422015 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-83 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-83 DE-188 |
| physical | 153 S. Ill., graph. Darst. : 21 cm |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| record_format | marc |
| spelling | Schumacher, Jörg Verfasser aut The phosphorylation induced activating mechanism of the response regulator FixJ vorgelegt von Jörg Schumacher 2000 153 S. Ill., graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1999 Genregulation (DE-588)4122166-7 gnd rswk-swf Rhizobium meliloti (DE-588)4178030-9 gnd rswk-swf Stickstofffixierung (DE-588)4183273-5 gnd rswk-swf Phosphorylierung (DE-588)4174459-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Rhizobium meliloti (DE-588)4178030-9 s Stickstofffixierung (DE-588)4183273-5 s Genregulation (DE-588)4122166-7 s Phosphorylierung (DE-588)4174459-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009119137&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schumacher, Jörg The phosphorylation induced activating mechanism of the response regulator FixJ Genregulation (DE-588)4122166-7 gnd Rhizobium meliloti (DE-588)4178030-9 gnd Stickstofffixierung (DE-588)4183273-5 gnd Phosphorylierung (DE-588)4174459-7 gnd |
| subject_GND | (DE-588)4122166-7 (DE-588)4178030-9 (DE-588)4183273-5 (DE-588)4174459-7 (DE-588)4113937-9 |
| title | The phosphorylation induced activating mechanism of the response regulator FixJ |
| title_auth | The phosphorylation induced activating mechanism of the response regulator FixJ |
| title_exact_search | The phosphorylation induced activating mechanism of the response regulator FixJ |
| title_full | The phosphorylation induced activating mechanism of the response regulator FixJ vorgelegt von Jörg Schumacher |
| title_fullStr | The phosphorylation induced activating mechanism of the response regulator FixJ vorgelegt von Jörg Schumacher |
| title_full_unstemmed | The phosphorylation induced activating mechanism of the response regulator FixJ vorgelegt von Jörg Schumacher |
| title_short | The phosphorylation induced activating mechanism of the response regulator FixJ |
| title_sort | the phosphorylation induced activating mechanism of the response regulator fixj |
| topic | Genregulation (DE-588)4122166-7 gnd Rhizobium meliloti (DE-588)4178030-9 gnd Stickstofffixierung (DE-588)4183273-5 gnd Phosphorylierung (DE-588)4174459-7 gnd |
| topic_facet | Genregulation Rhizobium meliloti Stickstofffixierung Phosphorylierung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009119137&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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