Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins:
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Jena
Urban und Fischer
2000
|
| Schriftenreihe: | Progress in histochemistry and cytochemistry
35,1 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturverz. S. 59 - 64 |
| Beschreibung: | 64 S. Ill., graph. Darst. : 24 cm |
Internformat
MARC
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| 100 | 1 | |a Helboe, Lone |e Verfasser |0 (DE-588)122148940 |4 aut | |
| 245 | 1 | 0 | |a Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins |c Lone Helboe ; Morten Møller |
| 264 | 1 | |a Jena |b Urban und Fischer |c 2000 | |
| 300 | |a 64 S. |b Ill., graph. Darst. : 24 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Progress in histochemistry and cytochemistry |v 35,1 | |
| 500 | |a Literaturverz. S. 59 - 64 | ||
| 650 | 7 | |a Receptoren |2 gtt | |
| 650 | 7 | |a Somatostatine |2 gtt | |
| 650 | 4 | |a Receptor antibodies | |
| 650 | 4 | |a Receptors, Somatostatin | |
| 650 | 4 | |a Somatostatin |x Receptors | |
| 650 | 0 | 7 | |a Immuncytochemie |0 (DE-588)4161368-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Somatostatin |0 (DE-588)4055484-3 |2 gnd |9 rswk-swf |
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| 689 | 0 | 2 | |a Immuncytochemie |0 (DE-588)4161368-5 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Møller, Morten |d 1942- |e Verfasser |0 (DE-588)122148959 |4 aut | |
| 830 | 0 | |a Progress in histochemistry and cytochemistry |v 35,1 |w (DE-604)BV000005277 |9 35,1 | |
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Datensatz im Suchindex
| _version_ | 1804128100439556096 |
|---|---|
| adam_text | Contents
1 Introduction 9
1.1 Somatostatin 9
1.2 Somatostatin receptors 10
2 Production of antibodies 13
2.1 Construction of recombinant plasmids 13
2.2 Transformation of bacteria 14
2.3 Expression of fusion proteins 14
2.4 Immunization 15
3 Screening of antisera 17
3.1 Membrane preparations 17
3.2 Western blot 17
4 Results of western blot analysis 19
5 Localization of somatostatin receptors by immunohistochemistry .... 25
5.1 Tissues and fixation of tissues 25
5.2 Antigen retrieval 25
5.3 Immunohistochemistry at the light microscopical level by using
enzyme labelling 26
5.4 Light microscopical double immunofluorescence 26
5.5 Visualization of somatostatin receptors at the electron microscopical
level 27
6 Localization of somatostatin receptors at the light
and electron microscopical level 29
6.1 Localization of somatostatin receptors in the brain 29
6.2 Localization of somatostatin receptors in the hypothalamus
and pituitary gland 40
6.2.1 Pituitary 41
6.2.2 Hypothalamus 41
6.3 Localization of somatostatin receptors in the retina 46
7 General comments and conclusion 55
7.1 Immunocytochemical visualization of somatostatin receptors 55
7.2 Biological function of somatostatin receptors 56
References 59
|
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| author | Helboe, Lone Møller, Morten 1942- |
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| callnumber-subject | QP - Physiology |
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| format | Book |
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| id | DE-604.BV013326222 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:43:52Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009088181 |
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| physical | 64 S. Ill., graph. Darst. : 24 cm |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| publisher | Urban und Fischer |
| record_format | marc |
| series | Progress in histochemistry and cytochemistry |
| series2 | Progress in histochemistry and cytochemistry |
| spelling | Helboe, Lone Verfasser (DE-588)122148940 aut Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins Lone Helboe ; Morten Møller Jena Urban und Fischer 2000 64 S. Ill., graph. Darst. : 24 cm txt rdacontent n rdamedia nc rdacarrier Progress in histochemistry and cytochemistry 35,1 Literaturverz. S. 59 - 64 Receptoren gtt Somatostatine gtt Receptor antibodies Receptors, Somatostatin Somatostatin Receptors Immuncytochemie (DE-588)4161368-5 gnd rswk-swf Somatostatin (DE-588)4055484-3 gnd rswk-swf Rezeptor (DE-588)4049722-7 gnd rswk-swf Somatostatin (DE-588)4055484-3 s Rezeptor (DE-588)4049722-7 s Immuncytochemie (DE-588)4161368-5 s DE-604 Møller, Morten 1942- Verfasser (DE-588)122148959 aut Progress in histochemistry and cytochemistry 35,1 (DE-604)BV000005277 35,1 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009088181&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Helboe, Lone Møller, Morten 1942- Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins Progress in histochemistry and cytochemistry Receptoren gtt Somatostatine gtt Receptor antibodies Receptors, Somatostatin Somatostatin Receptors Immuncytochemie (DE-588)4161368-5 gnd Somatostatin (DE-588)4055484-3 gnd Rezeptor (DE-588)4049722-7 gnd |
| subject_GND | (DE-588)4161368-5 (DE-588)4055484-3 (DE-588)4049722-7 |
| title | Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins |
| title_auth | Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins |
| title_exact_search | Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins |
| title_full | Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins Lone Helboe ; Morten Møller |
| title_fullStr | Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins Lone Helboe ; Morten Møller |
| title_full_unstemmed | Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins Lone Helboe ; Morten Møller |
| title_short | Localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins |
| title_sort | localization of somatostatin receptors at the light and electron microscopial level by using antibodies raised against fusion proteins |
| topic | Receptoren gtt Somatostatine gtt Receptor antibodies Receptors, Somatostatin Somatostatin Receptors Immuncytochemie (DE-588)4161368-5 gnd Somatostatin (DE-588)4055484-3 gnd Rezeptor (DE-588)4049722-7 gnd |
| topic_facet | Receptoren Somatostatine Receptor antibodies Receptors, Somatostatin Somatostatin Receptors Immuncytochemie Somatostatin Rezeptor |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009088181&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000005277 |
| work_keys_str_mv | AT helboelone localizationofsomatostatinreceptorsatthelightandelectronmicroscopiallevelbyusingantibodiesraisedagainstfusionproteins AT møllermorten localizationofsomatostatinreceptorsatthelightandelectronmicroscopiallevelbyusingantibodiesraisedagainstfusionproteins |