The ILT gene complex: from structure to function
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
München
Diss.-Verl. NG-Kopierladen
2000
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: München, Univ., Fak. für Biologie, Diss., 2000 |
| Beschreibung: | 139 S. Ill. : 21 cm |
| ISBN: | 3933214610 |
Internformat
MARC
| LEADER | 00000nam a22000008c 4500 | ||
|---|---|---|---|
| 001 | BV013325826 | ||
| 003 | DE-604 | ||
| 005 | 20001222 | ||
| 007 | t | ||
| 008 | 000829s2000 gw a||| m||| 00||| eng d | ||
| 016 | 7 | |a 959620508 |2 DE-101 | |
| 020 | |a 3933214610 |c kart. : DM 30.00 |9 3-933214-61-0 | ||
| 035 | |a (OCoLC)47315851 | ||
| 035 | |a (DE-599)BVBBV013325826 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-12 |a DE-19 | ||
| 100 | 1 | |a Torkar, Michaela |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a The ILT gene complex |b from structure to function |c von Michaela Torkar |
| 264 | 1 | |a München |b Diss.-Verl. NG-Kopierladen |c 2000 | |
| 300 | |a 139 S. |b Ill. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Zugl.: München, Univ., Fak. für Biologie, Diss., 2000 | ||
| 650 | 7 | |a Hochschulschrift |2 gtt | |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009087992&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-009087992 | ||
Datensatz im Suchindex
| _version_ | 1804128100067311617 |
|---|---|
| adam_text | Table of Contents
i
I Abstract 1
1 Introduction 2
1.1 The Immune System 2
1.2 Mechanisms of Innate Immune Recognition 3
1.3 Immune Evasion by Viruses 4
1.3.1 Missing self hypothesis 5
1.4 Natural Killer Cells: a Paradigm for the Regulation of Immune
Responses 6
1.4.1 Biology of NK cells 6
1.4.2 Cytotoxic activity of NK cells: Non MHC restricted pathways 7
1.5 NK Cell Receptors for MHC Class 1 9
1.5.1 Paired activation/inhibition receptor isoforms 9
1.5.2 Lectin like receptors 10
1.5.3 Ig like receptors 12
1.6 Ig Like Transcripts Modulate Function of Lymphoid and Myeloid Cells 14
1.6.1 Structural characteristics of the ILT receptor family 14
1.6.2 ILT expression patterns 16
1.6.3 Inhibition through ILTs 17
1.6.4 Activation through ILTs 18
1.6.5 ILT2 and 4 recognise HLA class I molecules 18
1.6.6 ILT homologues in mice 19
1.7 Expression Repertoires and Regulation of Gene Expression 20
1.8 Aim of Project: 21
2 Materials and Methods 23
2.1 Materials 23
2.1.1 Buffers and media 23
2.1.2 Oligonucleotide primers 23
2.1.3 Antibodies 25
2.1.4 Biotinylation of HP F1 monoclonal antibody 26
2.1.5 Cell lines and tissue culture techniques 26
2.2 DNA Analysis 28
2.2.1 PI artificial chromosome (PAC) library 28
2.2.2 Preparation of plasmid DNA from transformed bacterial cells 28
2.2.3 Transformation of E.coli 31
2.2.4 Fragmentation and sub cloning of PAC DNA 31
2.2.5 Agarose gel electrophoresis 33
2.2.6 Southern blots 33
2.2.7 Hybridisation and labelling of probes 34
2.2.8 Amplification of DNA by PCR 35
2.2.9 DNA sequencing 36
2.2.10 Fluorescence in situ hybridisation (FISH) 37
2.3 Construction and Analysis of Transgenic Mice 38
2.3.1 Preparation of the transgene 38
2.3.2 Slot blots 40
2.3.3 Extraction of DNA from tail biopsy 40
2.3.4 Flow cytometric analysis 41
2.4 RNA Analysis 41
2.4.1 RT PCR 41
2.4.2 Northern blots 42
2.5 Production of LIR6 Monoclonal Antibodies 43
2.5.1 Production of recombinant protein 43
2.5.2 Production and screening of monoclonal antibodies 48
3 Results 49
3.1 ILTs are Members of the Ig SF 49
3.1.1 Prediction of ILT structure based on amino acid sequences 49
3.1.2 ILT/LIR family: phylogeny and nomenclature 51
3.1.3 Design of gene specific PCR primers 54
3.2 Fine Map of the ILT/KIR Border 54
3.2.1 Chromosomal location of ILT and KIR genes on chromosome
19ql3.4 55
3.2.2 Identification of overlapping ILT encoding PAC clones by
restriction analysis 56
3.2.3 Identification of three ILT loci at the ILT/KIR border 58
3.3 Analysis of Two Novel ILT Loci: ILT9 and ILT10 62
3.3.1 ILT9 and ILT10 bear resemblance to activating ILTs 62
3.3.2 Expression of ILT9 and ILT10 65
3.4 Six ILT Genes are Grouped in ILT Cluster 1 67
3.4.1 Sequence analysis of PAC clone 52N12 67
3.4.2 Isotypic variation of ILT gene structures 70
3.4.3 Preliminary analysis of ILT promoter sequences 75
3.4.4 Sequence comparison of two different haplotypes 75
3.5 Characterisation of ILT Cluster II 79
3.5.1 Identification of ILT and LAIR loci outside the sequenced region 79
3.5.2 Haplotype specific variation in ILT cluster II 82
3.6 Overall Map of the Leukocyte Receptor Complex 84
3.7 PAC Clone 52N12 as a Transgenic Construct 88
3.8 Establishment of Transgenic Lines 90
3.8.1 Identification of one transgenic founder 90
3.8.2 Breeding of transgenic mice 91
3.8.3 Segregation of multiple integration sites 94
3.9 Expression of ILT2 and ILT3 in Transgenic Mice 96
3.9.1 Low level ILT2 expression on thymocytes 96
3.9.2 ILT2 expression on mouse B and NK cells 98
3.9.3 ILT3 expression on mouse B cells 100
4 Discussion 103
4.1 Evolution of the Leukocyte Receptor Complex: The Result of Extensive
Duplication Events? 104
4.1.1 Comparison of data with the Human Genome Project 104
4.1.2 Evolution of two ILT clusters: the result of an inversion? 105
4.1.3 Conserved 5 arrangement of genes encoded within the LRC
suggests common ancestor gene 106
4.1.4 Comparison of haplotype specific variations in the ILT and
the KIR gene clusters 109
4.1.5 The ILT region is more ancient than the KIR gene cluster 110
4.2 ILT Transgenic Mice as a Tool for Dissecting Gene Regulatory
Elements Ill
4.2.1 Expression of ILT2 and ILT3 in transgenic mice 112
4.2.2 The ILT transgene is not subject to negative position effects 113
4.2.3 DNAse I hypersensitivity site mapping as a means of
identifying gene regulatory elements 114
4.2.2 ILT transgenic mice for functional analysis 115
5 References 117
6 Abbreviations 136
7 Appendix 139
|
| any_adam_object | 1 |
| author | Torkar, Michaela |
| author_facet | Torkar, Michaela |
| author_role | aut |
| author_sort | Torkar, Michaela |
| author_variant | m t mt |
| building | Verbundindex |
| bvnumber | BV013325826 |
| ctrlnum | (OCoLC)47315851 (DE-599)BVBBV013325826 |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01237nam a22003258c 4500</leader><controlfield tag="001">BV013325826</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20001222 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">000829s2000 gw a||| m||| 00||| eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">959620508</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">3933214610</subfield><subfield code="c">kart. : DM 30.00</subfield><subfield code="9">3-933214-61-0</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)47315851</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV013325826</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">gw</subfield><subfield code="c">DE</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-12</subfield><subfield code="a">DE-19</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Torkar, Michaela</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">The ILT gene complex</subfield><subfield code="b">from structure to function</subfield><subfield code="c">von Michaela Torkar</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">München</subfield><subfield code="b">Diss.-Verl. NG-Kopierladen</subfield><subfield code="c">2000</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">139 S.</subfield><subfield code="b">Ill. : 21 cm</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Zugl.: München, Univ., Fak. für Biologie, Diss., 2000</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Hochschulschrift</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009087992&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-009087992</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV013325826 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:43:52Z |
| institution | BVB |
| isbn | 3933214610 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009087992 |
| oclc_num | 47315851 |
| open_access_boolean | |
| owner | DE-12 DE-19 DE-BY-UBM |
| owner_facet | DE-12 DE-19 DE-BY-UBM |
| physical | 139 S. Ill. : 21 cm |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| publisher | Diss.-Verl. NG-Kopierladen |
| record_format | marc |
| spelling | Torkar, Michaela Verfasser aut The ILT gene complex from structure to function von Michaela Torkar München Diss.-Verl. NG-Kopierladen 2000 139 S. Ill. : 21 cm txt rdacontent n rdamedia nc rdacarrier Zugl.: München, Univ., Fak. für Biologie, Diss., 2000 Hochschulschrift gtt (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009087992&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Torkar, Michaela The ILT gene complex from structure to function Hochschulschrift gtt |
| subject_GND | (DE-588)4113937-9 |
| title | The ILT gene complex from structure to function |
| title_auth | The ILT gene complex from structure to function |
| title_exact_search | The ILT gene complex from structure to function |
| title_full | The ILT gene complex from structure to function von Michaela Torkar |
| title_fullStr | The ILT gene complex from structure to function von Michaela Torkar |
| title_full_unstemmed | The ILT gene complex from structure to function von Michaela Torkar |
| title_short | The ILT gene complex |
| title_sort | the ilt gene complex from structure to function |
| title_sub | from structure to function |
| topic | Hochschulschrift gtt |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009087992&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT torkarmichaela theiltgenecomplexfromstructuretofunction |