Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2000
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Berlin, Techn. Univ., Diss., 2000 |
| Beschreibung: | XVI, 112 S. Ill., graph. Darst. |
Internformat
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| 100 | 1 | |a Schilling, Sabrina |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major |c vorgelegt von Sabrina Schilling |
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| 300 | |a XVI, 112 S. |b Ill., graph. Darst. | ||
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| 500 | |a Berlin, Techn. Univ., Diss., 2000 | ||
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Datensatz im Suchindex
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| adam_text |
TABLE OF CONTENTS:
ABBREVIATIONS.1
PREFACE.:.3
I. THEORETICAL PART.5
LEISHMANIASIS.:.5
1.1 THE DISEASE.5
1.2 THE PARASITE AND ITS LIFE CYCLE.8
1.2.1 LEISHMANIA PARASITES WITHIN THE SANDFLY VECTOR -
METACYCLOGENESIS.10
1.2.2 LEISHMANIA PARASITES WITHIN THE VERTEBRATE HOST.12
1.3 THE MURINE MODEL OF INFECTION WITH
L. MAJOR.15
1.3.1 THE GENETIC BACKGROUND.18
1.3.2 INNATE IMMUNE EFFECTOR MECHANISMS.18
1.3.2.1 INDUCIBLE NITRIC OXIDE SYNTHASE (INOS).18
1.3.2.2 NK CELLS.19
1.3.3 AQUIRED IMMUNE EFFECTOR MECHANISMS.21
1.3.3.1 THE ROLE OF B CELLS.21
1.3.3.2 THE ROLE OF T CELLS.22
I.3.3.2.1 THE CD4
+ T HELPER CELL SUBSETS.23
1.3.3.3 THE ROLE OF CYTOKINES.24
1.4 CONCLUSIONS.'.28
1.5 SUMMARY.29
II. PRACTICAL PART.31
THE ROLE OF T CELLS IN MICE INFECTED WITH THE INTRACELLULAR
PARASITE
L. MAJOR.31
II. 1 INTRODUCTION.31
II. 1.1 SCIENTIFIC BACKGROUND.31
II. 1.2 SCIENTIFIC AIMS.32
II. 1.3 EXPERIMENTAL DESIGN.33
II. 1.4 PLANS OF THE EXPERIMENTS - NETWORK.34
II.2 MATERIALS AND METHODS.37
IX
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/959695435
II .2.1 MATERIALS.37
11.2.1.1 MICE.37
11.2.1.2 PARASITES.37
11.2.1.3 PEPTIDES AND PROTEINS.37
11.2.1.4 ANTIBODIES.38
11.2.1.5 MEDIA.39
11.2.1.6 ANTIBIOTICS.39
11.2.1.7 CHEMICALS.39
11.2.1.8 EQUIPMENT.40
II.2.2 METHODS.41
11.2.2.1 MICE.41
11.2.2.1.1 SCREENING OF TRANSGENIC MICE.41
11.2.2.1.2 IMMUNIZATION.41
11.2.2.1.3 INFECTION WITH
L. MAJOR PROMASTIGOTES.42
11.2.2.2 THE RECOMBINANT
L. MAJOR PROMASTIGOTES.42
11.2.2.2.1 GENERATION OF RECOMBINANT
L. MAJOR PROMASTIGOTES.42
11.2.2.2.2 WESTERN BLOT ANALYSIS.42
11.2.2.2.3 CULTURE AND INFECTION OF MACROPHAGES.43
11.2.2.2.4 T CELL ASSAY.43
11.2.2.3 ADOPTIVE TRANSFER OF DOL 1.10 CELLS.44
11.2.2.4 FLOW CYTOMETRY.44
11.2.2.5 PREPARATION OF DERMAL CELLS FROM THE MOUSE EAR.44
\
11.2.2.6 IMMUNOHISTOCHEMISTRY.45
11.2.2.7 CFSE-STAINING.45
11.2.2.8 LIMITING DILUTION ASSAY.45
11.2.2.9 PCR FOR THE DETECTION OF
LEISHMANIA DNA.46
11.2.2.10 STIMULATION OF LN CELLS WITH SLA.46
11.2.2.11 ANALYSIS OF INTRACELLULAR CYTOKINE SECRETION.47
11.2.2.12 CYTOKINE-RELEASE ASSAYS.47
11.2.2.13 COMPETITIVE RT-PCR.47
11.2.2.14 STATISTICAL ANALYSIS.48
II.3 RESULTS.49
X
II.3.1 PROJECT 1: TRACKING PARASITE-SPECIFIC T HELPER CELLS IN MICE
INFECTED WITH L.
MAJOR.49
11.3.1.1 THE RECOMBINANT PARASITE.49
11.3.1.2 C5.6 PROMASTIGOTES INDUCE OVA-SPECIFIC T CELL RESPONSES.52
11.3.1.3 FATE OF OVA-SPECIFIC T CELLS IN OVA-IMMUNIZED MICE.54
11.3.1.4 OVA-SPECIFIC T CELLS IN MICE INFECTED WITH C5.6 PROMASTIGOTES
.56
11.3.1.5 TRACKING OVA-SPECIFIC T CELLS IN MICE IMMUNIZED WITH OVA ANEK
SUBSEQUENTLY INFECTED WITH C5.6 PROMASTIGOTES.63
11.3.1.6 OVA-SPECIFIC T CELLS DO NOT INDUCE A PROTECTIVE RESPONSE IN
MICE INFECTED
WITH C5.6 PROMASTIGOTES.68
II.3.2 PROJECT 2: THE ROLE OF LACK-SPECIFIC T CELLS IN THE
SUSCEPTIBILITY OF BALB/C
MICE INFECTED WITH L. MAJOR.70
11.3.2.1 EARLY PARASITE DISSEMINATION IS BETTER CONTROLLED IN
LACK-TOLERANT MICE70
11.3.2.2 THE NUMBER OF PARASITES PRESENT IN THE INFLAMMATORY SITE IS
DECREASED IN
BALB/C MICE WHICH ARE TOLERANT TO LACK.72
11.3.2.3 LACK-SPECIFIC T CELLS ARE RAPIDLY RECRUITED TO INFLAMMATORY
SITES IN
BALB/C MICE INFECTED WITH
L. MAJOR.74
11.3.2.4 LACK-SPECIFIC CD4
+ T CELLS ACCOUNT FOR A SIGNIFICANT FRACTION OF IL-4-
SECRETING CELLS EARLY AFTER INFECTION.77
II
.4
DISCUSSION.
81
II.4.1 PROJECT 1:.81
II.4.1.1 C5.6 PROMASTIGOTES EXPRESSED OVALBUMIN WHICH WAS PROCESSED BY
ANTIGEN
PRESENTING CELLS AND PRESENTED TO CD4
+ T CELLS IN VIVO.81
II.4.1.2 OVA-SPECIFIC T CELLS IN SUSCEPTIBLE BALB/C MICE INFECTED WITH
L. MAJOR
.83
II.4.2 PROJECT 2:.86
11.4.2.1 LACK-SPECIFIC T CELLS PLAY AN EARLY ROLE IN THE SUSCEPTIBILITY
OF BALB/C
MICE INFECTED WITH
L. MAJOR.86
11.4.2.2 THE EARLY EVENTS AFTER INFECTION WITH
L. MAJOR.87
11.4.2.2.1 THE INNATE IMMUNE RESPONSE TO
L. MAJOR.87
11.4.2.2.2 THE ACQUIRED IMMUNE RESPONSE TO
L. MAJOR.89
11
.5
SUMMARY.95
11.6
ZUSAMMENFASSUNG.
97
11
.7
REFERENCES.99
XI |
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| spelling | Schilling, Sabrina Verfasser aut Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major vorgelegt von Sabrina Schilling 2000 XVI, 112 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Berlin, Techn. Univ., Diss., 2000 Hochschulschrift (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009080578&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schilling, Sabrina Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major Hochschulschrift |
| subject_GND | (DE-588)4113937-9 |
| title | Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major |
| title_auth | Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major |
| title_exact_search | Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major |
| title_full | Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major vorgelegt von Sabrina Schilling |
| title_fullStr | Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major vorgelegt von Sabrina Schilling |
| title_full_unstemmed | Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major vorgelegt von Sabrina Schilling |
| title_short | Fate and role of parasite-specific T helper lymphocytes in BALB c mice infected with the intracellular parasite L. major |
| title_sort | fate and role of parasite specific t helper lymphocytes in balb c mice infected with the intracellular parasite l major |
| topic | Hochschulschrift |
| topic_facet | Hochschulschrift |
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