Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT]:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
2000
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Jena, Univ., Diss., 2000 |
| Beschreibung: | II, 101 Bl. graph. Darst. : 30 cm |
Internformat
MARC
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| 245 | 1 | 0 | |a Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT] |c von Haijun Chen |
| 264 | 1 | |c 2000 | |
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| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Jena, Univ., Diss., 2000 | ||
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Datensatz im Suchindex
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|---|---|
| adam_text |
CONTENTS
SUMMARY J
1. INTRODUCTION
1.1 VOLTAGE-GATED SODIUM CHANNELS 1
1.2 PRIMARY STRUCTURE OF NA CHANNELS 2
1.3 GENE FAMIIY OF CLONED MAMMALIAN NA CHANNELS 6
1.4 MODULATION OF NA CHANNELS BY NEUROTOXINS 6
1.4.1 NEUROTOXIN GROUPS 6
1.4.2 SCORPION A-TOXINS 8
1.5 OBJECTIVES 10
1.5.1 MODE OF ACTION OF LQH II, LQH III, AND LQHALT IN A SINGLE TYPE OF
CLONED NA CHANNELS 10
1.5.2 MECHANISM OF A-TOXIN BINDING AND ACTION 10
1.5.3 MOLECULAR BASIS FOR THE TOXICITIES OF LQH II, LQH III, AND LQHALT
11
2. MATERIALS AND METHODS 13
2.1 ISOLATION OF PLASMID DNAS 13
2.2. CELL CULTURE 13
2.3. TRANSFECTION 14
2.3.1 TRANSIENT TRANSFECTION OF HEK 293 CELLS WITH PL. HH1, RBLL
AND HPN1 CHANNEL GENES 14
2.3.2 STABLE EXPRESSION OF PL AND HH1 CHANNELS IN HEK 293 CELLS 15
2.4 ELECTROPHYSIOLOGICAL MEASUREMENTS 15
2.4.1 WHOLE-CELL VOLTAGE CLAMP 15
2.4.2 TOXIN APPLICATION 15
2.5. RECORDING PROTOCOLS AND DATA ANALYSIS 17
2.5.1 PROTOCOLS FOR RECORDING AND ANALYSIS OF CURRENT-VOLTAGE
RELATIONSHIPS 1
7
2.5.2 PROTOCOLS FOR RECORDING AND ANALYSIS OF STEADY-STATE
FAST AND SLOW INACTIVATION 1
8
2.5.3 ASSAY FOR THE DEGREE OF FAST INACTIVATION 19
2.5.4 KINETICS OF FAST INACTIVATION AND RECOVERY FROM INACTIVATION 19
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/959282408
2.5.5 DOSE-RESPONSE CURVES OF TOXIN EFFECTS 20
2.5.6 NOISE ANALYSIS 20
2.5.7 PROTOCOLS FOR THE RECORDING AND ANALYSIS OF TOXIN DISSOCIATION
AND ASSOCIATION KINETICS 21
2.5.8 VOLTAGE DEPENDENCE AND GATING CHARGE 21
3. RESULTS 23
3.1 MODULATION OF CLONED PL NA CHANNELS BY LQH II, LQH III,
AND LQHALT AND BINDING PROPERTIES OF THE TOXINS 23
3.1.1 EFFECTS OF LQH II, LQH III AND LQHALT ON ACTIVATION AND
FAST INACTIVATION OF PL CHANNELS 23
3.1.2 KINETICS OF TOXIN ASSOCIATION AND DISSOCIATION FOR PL CHANNELS 32
3.2 MODULATION OF HH1 CHANNELS BY LQH II, LQH III AND LQHALT AND
BINDING PROPERTIES OF THE TOXINS 36
3.2.1 EFFECTS OF LQH II, LQH III AND LQHALT ON ACTIVATION AND FAST
INACTIVATION OF HH1 CHANNELS 36
3.2.2 KINETICS OF TOXIN ASSOCIATION AND DISSOCIATION IN HH1 CHANNELS 41
3.3 MODULATION OF SLOW INACTIVATION OF NA CHANNELS BY LQH II AND
LQH III
44
3.3.1 LQH II AND LQH III ALTER THE VOLTAGE DEPENDENCE OF STEADY-STATE
SLOW INACTIVATION IN PL AND HH1 CHANNELS 45
3.3.2 EFFECTS OF LQH III ON THE DEVELOPMENT OF SLOW INACTIVATION AND
ON THE RECOVERY FROM SLOW INACTIVATION OF HH1 CHANNELS 48
3.3.3 VOLTAGE-DEPENDENT BINDING OF LQH II AND LQH III TO PL AND HH1
CHANNELS 51
3.4 TOXIN ACTIVITIES ON TWO NA CHANNEL TYPES FROM THE CNS
AND THE PNS 54
3.4.1 TOXIN EFFECTS ON ACTIVATION AND FAST INACTIVATION OF RBLL CHANNELS
54
3.4.2 TOXIN EFFECTS ON ACTIVATION AND FAST INACTIVATION OF
HPN1 CHANNELS 59
4. DISCUSSION AND CONCLUSIONS
4.1 TOXIN MODULATION OF NA CHANNEL FUNCTIONS 56
4.1.1 LQH II, LQH III, AND LQHALT MODIFY ACTIVATION AND FAST
INACTIVATION OF NA CHANNELS 66
4.1.2 LQH
II, LQH III AND LQHALT REDUCE THE VOLTAGE DEPENDENCE OF
NA CHANNELS DURING FAST CONFORMATIONAL CHANGES 67
4.1.3 MODULATION OF SLOW INACTIVATION OF PL AND HH1 CHANNELS BY
LQH II AND LQH III 69
4.2 BINDING PROPERTIES OF THE TOXINS IN PL AND HH1 CHANNELS 70
4.3 VOLTAGE DEPENDENCIES OF TOXIN BINDING AND SLOW INACTIVATION
OF NA CHANNELS 72
4.4 MOLECULAR BASIS FOR THE TOXICITIES OF LQH II, LQH
III, AND LQHALT
IN MAMMALS 74
4.4.1 DIFFERENT TOXICITIES OF LQH II, LQH III, AND LQHALT IN THE CNS 74
4.4.2 SIMILAR TOXICITIES OF LQH II, LQH III, AND LQHALT IN THE
PERIPHERAL
SYSTEM 75
4.5 SPECIFIC INTERACTIONS BETWEEN THE NA CHANNEL AND THE THREE
A-TOXINS 76
4.5.1 LQH HL MAY HAVE TWO BINDING SITES IN HH1 CHANNELS 76
4.5.2 LQH III DISTINGUISHES NA CHANNEL TYPES FROM THE CNS
AND THE PNS 76
4.5.3 LQH II DELAYS THE ACTIVATION KINETICS OF HPN1 CHANNELS 78
4.5.4 MOLECULAR BASIS FOR THE LESS TOXICITY OF LQHALT IN MAMMALS 78
4.6 CONCLUSIONS 79
5. REFERENCES
6. APPENDIX 95
6.1 ABBREVIATION
95
6.2 ACKNOWLEDGEMENT
96
6.3 PUBLICATIONS
97
6.4 RESUME
98 |
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| author | Chen, Haijun 1966- |
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| bvnumber | BV013292416 |
| ctrlnum | (OCoLC)247075094 (DE-599)BVBBV013292416 |
| format | Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV013292416 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T18:11:14Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009062294 |
| oclc_num | 247075094 |
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| physical | II, 101 Bl. graph. Darst. : 30 cm |
| publishDate | 2000 |
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| spelling | Chen, Haijun 1966- Verfasser (DE-588)122141733 aut Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT] von Haijun Chen 2000 II, 101 Bl. graph. Darst. : 30 cm txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 2000 (DE-588)4113937-9 Hochschulschrift gnd-content DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009062294&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chen, Haijun 1966- Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT] |
| subject_GND | (DE-588)4113937-9 |
| title | Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT] |
| title_auth | Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT] |
| title_exact_search | Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT] |
| title_full | Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT] von Haijun Chen |
| title_fullStr | Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT] von Haijun Chen |
| title_full_unstemmed | Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT] von Haijun Chen |
| title_short | Modulation von geklonten spannungsabhängigen Natriumkanälen durch die Skorpion-a-Toxine [Skorpion alpha Toxine] Lqh II, Lqh III, und LqhaIT [Lqh alpha IT] |
| title_sort | modulation von geklonten spannungsabhangigen natriumkanalen durch die skorpion a toxine skorpion alpha toxine lqh ii lqh iii und lqhait lqh alpha it |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009062294&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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