Stress response: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2000
|
| Schriftenreihe: | Methods in molecular biology
99 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVI, 488 S. Ill., graph. Darst. |
| ISBN: | 0896036111 |
Internformat
MARC
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| 245 | 1 | 0 | |a Stress response |b methods and protocols |c ed. by Stephen M. Keyse |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2000 | |
| 300 | |a XVI, 488 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in molecular biology |v 99 | |
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| 650 | 4 | |a Cellular signal transduction |v Laboratory manuals | |
| 650 | 4 | |a Gene Expression |v Laboratory Manuals | |
| 650 | 4 | |a Gene expression |v Laboratory manuals | |
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| 650 | 4 | |a Signal Transduction |v Laboratory Manuals | |
| 650 | 4 | |a Stress (Physiology) |v Laboratory manuals | |
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Datensatz im Suchindex
| _version_ | 1804128018952617984 |
|---|---|
| adam_text | Contents
Preface v
Contributors xiii
Part I. Detection and Assay of Stress Induced Damage
1 Identifying and Counting Protein Modifications Triggered
by Nitrosative Stress
Prabodh K. Sehajpal and Harry M. Lander 3
2 Determination of Carbonyl Groups in Oxidized Proteins
Rodney L. Levine, Nancy Wehr, Joy A. Williams,
Earl Ft. Stadtman, and Emily Shacter 15
3 Quantitation of 4 Hydroxynonenal Protein Adducts
Koji Uchida and Earl R. Stadtman 25
4 Detection of Oxidative Stress in Lymphocytes Using
Dichlorodihydrofluorescein Diacetate
Cecile M. Krejsa and Gary L. Schieven 35
5 The Measurement of Protein Degradation in Response
to Oxidative Stress
Thomas Reinheckel, Tilman Grune,
and Kelvin J. A. Davies 49
Part II. The Activation of Signal Transduction by Cellular Stress
6 Analysis of the Role of the AMP Activated Protein Kinase
in the Response to Cellular Stress
D. Grahame Hardie, Ian P. Salt, and Stephen P. Davies 63
7 Detection and Activation of Stress Responsive Tyrosine Kinases
Gary L. Schieven 75
8 Detection of DNA Dependent Protein Kinase in Extracts
from Human and Rodent Cells
Yamini Achari and Susan P. Lees Miller 85
9 Expression and Assay of Recombinant ATM
Yael Ziv, Sharon Banin, Dae Sik Lim, Christine E. Canman,
Michael B. Kastan, and Yosef Shiloh 99
10 Detection and Purification of a Multiprotein Kinase Complex
from Mammalian Cells: IKK Signalsome
Frank Mercurio, David B. Young, and Anthony M. Manning 109
11 Methods to Assay Stress Activated Protein Kinases
Ana Cuenda 127
12 Monitoring the Activation of Stress Activated Protein Kinases
Using GAL4 Fusion Transactivators
Chao Feng Zheng and Li Xu 745
13 Use of Kinase Inhibitors to Dissect Signaling Pathways
Ana Cuenda and Dario R. Alessi 161
14 The Development and Use of Phospho Specific Antibodies
to Study Protein Phosphorylation
Jeremy P. Blaydes, Borek Vojtesek, Graham B. Bloomberg,
and Ted R. Hupp 177
15 Peptide Assay of Protein Kinases and Use of Variant Peptides
to Determine Recognition Motifs
D. Grahame Hardie 191
Part III. The Analysis of Stress Induced Gene Expression
16 Assaying NF kB and AP 1 DNA Binding
and Transcriptional Activity
Judith M. Mueller and Heike L. Pahl 205
17 Analysis of the Mammalian Heat Shock Response:
Inducible Gene Expression and Heat Shock Factor Activity
Anu Mathew, Yanhong Shi, Caroline Jolly,
and Richard I. Morimoto 217
18 Approaches to Define the Involvement of Reactive Oxygen
Species and Iron in Ultraviolet A Inducible Gene Expression
Charareh Pourzand, Olivier Reelfs, and Rex M. Tyrrell 257
19 The Human Immunodeficiency Virus LTR Promoter Region
as a Reporter of Stress Induced Gene Expression
Michael W. Bate, Sushma R. Jassal, and David W. Brighty 277
20 SAGE: The Serial Analysis of Gene Expression
Jill Powell 297
21 Analysis of Differential Gene Expression Using the SABRE
Enrichment Protocol
Daniel J. Lavery, Phillippe Fonjallaz, Fabienne Fleury Olela,
and Ueli Schibler 321
22 UVB Regulated Gene Expression in Human Keratinocytes:
Analysis by Differential Display
Harry Frank Abts, Thomas Weiss, Kai Breuhahn,
and Thomas Ruzicka 347
Part IV. Analysis of Stress Protein Function
23 Heme Oxygenase Activity: Current Methods and Applications
Stefan W. Ryter, Egil Kvam, and Rex M. Tyrrell 369
24 Analysis of Molecular Chaperone Activities Using In Vitro
and In Vivo Approaches
Brian C. Freeman, Annamieke Michels, Jaewhan Song,
Harm H. Kampinga, and Richard I. Morimoto 393
25 Analysis of Chaperone Properties of Small Hsp s
Monika Ehrnsperger, Matthias Gaestel,
and Johannes Buchner 421
26 Analysis of Small Hsp Phosphorylation
Rainer Benndorf, Katrin Engel, and Matthias Gaestel 431
27 Analysis of Multisite Phosphorylation of the p53
Tumor Suppressor Protein by Tryptic Phosphopeptide Mapping
David W. Meek and Diane M. Milne 447
28 The Development of Physiological Models to Study Stress
Protein Responses
Ted R. Hupp 465
Index 485
|
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| spelling | Stress response methods and protocols ed. by Stephen M. Keyse Totowa, NJ Humana Press 2000 XVI, 488 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 99 Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cellular signal transduction Laboratory manuals Gene Expression Laboratory Manuals Gene expression Laboratory manuals Heat shock proteins Laboratory manuals Heat-Shock Proteins analysis Laboratory Manuals Molecular Biology Laboratory Manuals Molecular biology Laboratory manuals Signal Transduction Laboratory Manuals Stress (Physiology) Laboratory manuals Stress, Physiological Laboratory Manuals Stressreaktion (DE-588)4138565-2 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Stressreaktion (DE-588)4138565-2 s Genexpression (DE-588)4020136-3 s DE-604 Signaltransduktion (DE-588)4318717-1 s Keyse, Stephen M. Sonstige oth Methods in molecular biology 99 (DE-604)BV035362695 99 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009035305&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Stress response methods and protocols Methods in molecular biology Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cellular signal transduction Laboratory manuals Gene Expression Laboratory Manuals Gene expression Laboratory manuals Heat shock proteins Laboratory manuals Heat-Shock Proteins analysis Laboratory Manuals Molecular Biology Laboratory Manuals Molecular biology Laboratory manuals Signal Transduction Laboratory Manuals Stress (Physiology) Laboratory manuals Stress, Physiological Laboratory Manuals Stressreaktion (DE-588)4138565-2 gnd Genexpression (DE-588)4020136-3 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| subject_GND | (DE-588)4138565-2 (DE-588)4020136-3 (DE-588)4318717-1 |
| title | Stress response methods and protocols |
| title_auth | Stress response methods and protocols |
| title_exact_search | Stress response methods and protocols |
| title_full | Stress response methods and protocols ed. by Stephen M. Keyse |
| title_fullStr | Stress response methods and protocols ed. by Stephen M. Keyse |
| title_full_unstemmed | Stress response methods and protocols ed. by Stephen M. Keyse |
| title_short | Stress response |
| title_sort | stress response methods and protocols |
| title_sub | methods and protocols |
| topic | Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Cellular signal transduction Laboratory manuals Gene Expression Laboratory Manuals Gene expression Laboratory manuals Heat shock proteins Laboratory manuals Heat-Shock Proteins analysis Laboratory Manuals Molecular Biology Laboratory Manuals Molecular biology Laboratory manuals Signal Transduction Laboratory Manuals Stress (Physiology) Laboratory manuals Stress, Physiological Laboratory Manuals Stressreaktion (DE-588)4138565-2 gnd Genexpression (DE-588)4020136-3 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| topic_facet | Wetenschappelijke technieken Wissenschaftliches Arbeiten Cellular signal transduction Laboratory manuals Gene Expression Laboratory Manuals Gene expression Laboratory manuals Heat shock proteins Laboratory manuals Heat-Shock Proteins analysis Laboratory Manuals Molecular Biology Laboratory Manuals Molecular biology Laboratory manuals Signal Transduction Laboratory Manuals Stress (Physiology) Laboratory manuals Stress, Physiological Laboratory Manuals Stressreaktion Genexpression Signaltransduktion |
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