Construction of Onchocerca volvulus hybrid proteins for diagnosis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2000
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | V, 94 S. Ill., graph. Darst. |
Internformat
MARC
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| 041 | 0 | |a eng | |
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| 100 | 1 | |a Nde, Pius Nche |d 1963- |e Verfasser |0 (DE-588)122268385 |4 aut | |
| 245 | 1 | 0 | |a Construction of Onchocerca volvulus hybrid proteins for diagnosis |c von Pius Nche Nde |
| 264 | 1 | |c 2000 | |
| 300 | |a V, 94 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Berlin, Humboldt-Univ., Diss., 2000 | ||
| 650 | 0 | 7 | |a Serodiagnostik |0 (DE-588)4181044-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a B-Lymphozyt |0 (DE-588)4137121-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Onchocerca volvulus |0 (DE-588)4210380-0 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Onchocerca volvulus |0 (DE-588)4210380-0 |D s |
| 689 | 0 | 1 | |a B-Lymphozyt |0 (DE-588)4137121-5 |D s |
| 689 | 0 | 2 | |a Serodiagnostik |0 (DE-588)4181044-2 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009022191&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-009022191 | |
Datensatz im Suchindex
| _version_ | 1807683295882772480 |
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| adam_text |
TABLE
OF
CONTENTS
DEDICATION.
I
AKNO
WLEDGEMENTS
.
II
SUMMARY.
IV
ZUSAMMENFASSUNG
.
V
1
INTRODUCTION
.
1
1.1
THE
BIOLOGY
OF
O.
VOLVULUS
.
1
1.2
ONCHOCERCA
PATHOLOGY
.
3
1.3
DIAGNOSIS,
CONTROL
AND
TREATMENT
OF
ONCHOCERCIASIS
.
3
1.4
THE
20.5
KDA
PROTEIN
(OV
20)
OF
O.
VOLVULUS
.
6
1.5
THE
33
KDA
PROTEIN
(OV
33)
OF
O.
VOLVULUS.
7
1.6
AIM
AND
OBJECTIVES
.
8
2
RESULTS
.
10
2.1
IDENTIFICATION
OF
B-CELL
EPITOPES
OF
OV
33
AND
OV
20
PROTEINS
.
10
2.1.2
MAPPING
THE
B-CELL
EPITOPES
OF
OV
33
.
10
2.1.3
MAPPING
THE
B-CELL
EPITOPES
OF
OV
20
.
11
2.2
'
CONSTRUCTION
AND
EXPRESSION
OF
OVHL
HYBRID
PROTEIN
.
13
2.2.1
DESIGN
OF
OVHL
AND
ANALYSIS
OF
THE
AMINO
ACID
SEQUENCE
.
13
2.2.2
PRIMER
DESIGN
.
14
2.2.3
EXPRESSION
AND
PURIFICATION
OF
OVH
1
.
15
2.3
IMMUNOLOGICAL
CHARACTERISATION
OF
OVH
1
.
16
2.3.1
REACTIVITY
OF
ONCHOCERCIASIS
PATIENT
SERA
TO
OVHL
.
16
2.3.2
T
CELL
PROLIFERATION
ASSAYS
WITH
RECOMBINANT
OVHL
ANTIGEN
.
19
2.3.3
POLYCLONAL
STIMULATION
OF
PBMC
.
21
2.3.4
CONCENTRATION
OF
CYTOKINES
IN
CELL
CULTURE
SUPERNATANTS
.
22
2.3.5
EVALUATION
OF
OVHL
IMMUNOGENICITY
IN
THE
MOUSE
MODEL
.
23
2.4
CONSTRUCTION,
EXPRESSION
AND
CHARACTERISATION
OF
OVH2
AND
OVH3
HYBRID
PROTEINS.
.23
2.4.1
EXPRESSION
AND
PURIFICATION
OF
OVH2
AND
OVH3
HYBRID
PROTEINS
.
24
2.5
REACTIVITY
OF
ONCHOCERCIASIS
AND
LYMPHATIC
FILARIASIS
PATIENT
SERA
AGAINST
OVH2
AND
OVH3
.
26
2.5.1
CHARACTERISATION
OF
SERA
AND
THEIR
DONORS
.
26
2.5.2
EVALUATION
OF
ANTIBODY
RESPONSES
TO
OVH2
.
27
2.5.3
EVALUATION
OF
ANTIBODY
RESPONSES
TO
OVH3
.
31
2.5.4
EVALUATION
OF
ANTIBODY
RESPONSES
TO
OVAG
.
35
3
DISCUSSION
.
37
3.1
IGG4
EPITOPES
OF
OV
33
.
38
3.1.2
IGG4
EPITOPES
OF
OV
20
.
38
3.1.3
CONSTRUCTION
AND
EXPRESSION
OF
OVH
1
.
39
3.1.4
ANTIBODY
RESPONSES
TO
OVH
1
.
40
3.1.5
CELLULAR
IMMUNE
RESPONSES
TO
OVH
1
.
41
3.2
CONSTRUCTION,
EXPRESSION
AND
PURIFICATION
OF
OVH2
AND
OVH3
HYBRID
PROTEINS
.
42
3.3
EVALUATION
OF
ANTIBODY
RESPONSES
TO
OVH2
AND
OVH3
HYBRID
PROTEINS
.
43
3.3.1
THE
POTENTIAL
OF
OVH2
AND
OVH3
PROTEINS
FOR
THE
DIAGNOSIS
OF
ONCHOCERCIASIS
.
44
4
METHODS
.
47
4.1
MOLECULAR
BIOLOGY
METHODS
.
47
4.1.1
POLYMERASE
CHAIN
REACTION
(PCR)
.
47
4.1.2
PURIFICATION
AND
CONCENTRATION
OF
DNA
.
48
4.1.3
DETERMINATION
OF
CONCENTRATION
AND
QUALITY
OF
DNA
.
50
4.1.4
ENZYMATIC
MANIPULATION
OF
DNA
.
51
4.1.5
AGAROSE
GEL
ELECTROPHORESIS
OF
DNA
.
52
4.1.6
PREPARATION
OF
COMPETENT
BACTERIA
.
52
4.1.7
TRANSFORMATION
OF
COMPETENT
BACTERIA
.
52
4.1.8
ANALYSIS
OF
TRANSFORMED
BACTERIA
.
53
4.1.9
BACTERIAL
CULTURE
.
54
4.1.10
SEQUENCING
OF
PLASMID
DNA
.
54
4.1.11
SOLUBILITY
ASSAY
OF
EXPRESSED
RECOMBINANT
PROTEIN
.
54
4.1.12
LARGE
SCALE
EXPRESSION
AND
PURIFICATION
OF
HETEROLOGOUS
PROTEINS
.
55
4.1.13
STORAGE
OF
BACTERIAL
STOCKS.
56
4.2 BIOCHEMICAL
METHODS.
57
4.2.1
PREPARATION
OF
O.
VOLVULUS
ANTIGEN
EXTRACT
(OVAG)
.
I
.
57
4.2.2
DETERMINATION
OF
PROTEIN
CONCENTRATION
.
57
4.2.3
SDS-POLYACRYLAMIDE
GEL
ELECTROPHORESIS
(SDS-PAGE)
.
58
4.2.4
ELECTROPHORETIC
TRANSFER
OF
PROTEINS
TO
NITROCELLULOSE
MEMBRANES
.
58
4.3 IMMUNOLOGICAL
AND
CELLULAR
METHODS
.
59
4.3.1
IMMUNODETECTION
OF
PEPTIDES
ON
CELLULOSE
MEMBRANES.
59
4.3.2
ENZYME-LINKED
IMMUNOSORBENT
ASSAY
.
59
4.3.3
IMMUNISATION
OF
BALB/C
MICE
AND
PREPARATION
OF
SERA
.
61
4.3.4
PURIFICATION
OF
PERIPHERAL
BLOOD
MONONUCLEAR
CELLS
(PBMC)
.
62
4.3.5
CELLULAR
PROLIFERATION
ASSAYS
.
62
4.3.6
GENERATION
OF
CYTOKINES
IN
VITRO
.
63
4.3.7
DETERMINATION
OF
CYTOKINE
CONCENTRATION
IN
CELL
CULTURE
SUPERNATANTS.
64
4.3.8
LOCATION
OF
THE
STUDY
SITE
.
64
4.4
COMPUTER
ANALYSIS
OF
AMINO
ACID
AND
NUCLEIC
ACID
SEQUENCES.
64
5
MATERIALS
.
65
6
ABBREVIATIONS
.
73
7
REFERENCES
.
75
8
CURRICULUM
VITAE
.
89
9
PUBLICATIONS
AND
CONFERENCE
ABSTRACTS
.
91 |
| any_adam_object | 1 |
| author | Nde, Pius Nche 1963- |
| author_GND | (DE-588)122268385 |
| author_facet | Nde, Pius Nche 1963- |
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| author_sort | Nde, Pius Nche 1963- |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV013239465 |
| illustrated | Illustrated |
| indexdate | 2024-08-18T00:32:09Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-009022191 |
| oclc_num | 247444162 |
| open_access_boolean | |
| owner | DE-91 DE-BY-TUM DE-355 DE-BY-UBR DE-11 DE-188 |
| owner_facet | DE-91 DE-BY-TUM DE-355 DE-BY-UBR DE-11 DE-188 |
| physical | V, 94 S. Ill., graph. Darst. |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| record_format | marc |
| spelling | Nde, Pius Nche 1963- Verfasser (DE-588)122268385 aut Construction of Onchocerca volvulus hybrid proteins for diagnosis von Pius Nche Nde 2000 V, 94 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Berlin, Humboldt-Univ., Diss., 2000 Serodiagnostik (DE-588)4181044-2 gnd rswk-swf B-Lymphozyt (DE-588)4137121-5 gnd rswk-swf Onchocerca volvulus (DE-588)4210380-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Onchocerca volvulus (DE-588)4210380-0 s B-Lymphozyt (DE-588)4137121-5 s Serodiagnostik (DE-588)4181044-2 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009022191&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Nde, Pius Nche 1963- Construction of Onchocerca volvulus hybrid proteins for diagnosis Serodiagnostik (DE-588)4181044-2 gnd B-Lymphozyt (DE-588)4137121-5 gnd Onchocerca volvulus (DE-588)4210380-0 gnd |
| subject_GND | (DE-588)4181044-2 (DE-588)4137121-5 (DE-588)4210380-0 (DE-588)4113937-9 |
| title | Construction of Onchocerca volvulus hybrid proteins for diagnosis |
| title_auth | Construction of Onchocerca volvulus hybrid proteins for diagnosis |
| title_exact_search | Construction of Onchocerca volvulus hybrid proteins for diagnosis |
| title_full | Construction of Onchocerca volvulus hybrid proteins for diagnosis von Pius Nche Nde |
| title_fullStr | Construction of Onchocerca volvulus hybrid proteins for diagnosis von Pius Nche Nde |
| title_full_unstemmed | Construction of Onchocerca volvulus hybrid proteins for diagnosis von Pius Nche Nde |
| title_short | Construction of Onchocerca volvulus hybrid proteins for diagnosis |
| title_sort | construction of onchocerca volvulus hybrid proteins for diagnosis |
| topic | Serodiagnostik (DE-588)4181044-2 gnd B-Lymphozyt (DE-588)4137121-5 gnd Onchocerca volvulus (DE-588)4210380-0 gnd |
| topic_facet | Serodiagnostik B-Lymphozyt Onchocerca volvulus Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=009022191&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ndepiusnche constructionofonchocercavolvulushybridproteinsfordiagnosis |