Practical problem solving in HPLC:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Weinheim [u.a.]
Wiley-VCH
2000
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 178 S. graph. Darst. |
| ISBN: | 3527298428 |
Internformat
MARC
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| 100 | 1 | |a Kromidas, Stavros |d 1954- |e Verfasser |0 (DE-588)122124685 |4 aut | |
| 240 | 1 | 0 | |a HPLC-Tips |
| 245 | 1 | 0 | |a Practical problem solving in HPLC |c Stavros Kromidas |
| 264 | 1 | |a Weinheim [u.a.] |b Wiley-VCH |c 2000 | |
| 300 | |a XV, 178 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 7 | |a HPLC |2 gtt | |
| 650 | 7 | |a Probleemoplossing |2 gtt | |
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| 650 | 0 | 7 | |a Fehlersuche |0 (DE-588)4016615-6 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a HPLC |0 (DE-588)4072641-1 |D s |
| 689 | 0 | 1 | |a Fehlersuche |0 (DE-588)4016615-6 |D s |
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Datensatz im Suchindex
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|---|---|
| adam_text |
CONTENTS
I.
I.I
1.2
1.3
1.4
1.5
1.6
2.
TIP
NO.
01
02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
17
18
3.
19
20
21
22
INTRODUCTION
1
HOW
TO
USE
THIS
BOOK
1
HPLC
-
THE
DEVELOPMENT
OF
A
NAME
2
FREQUENTLY
USED
ABBREVIATIONS
AND
SYMBOLS
IN
THIS
BOOK
4
GENERAL
TIPS
FOR
NEWCOMERS
5
CHECK
LIST
FOR
REVERSED-PHASE
HPLC
10
SOME
IMPORTANT
CHROMATOGRAPHIC
TERMS
13
SIMPLE
TESTS
AND
DECISION
CRITERIA
15
WHAT
DOES
THE
NAME
OF
A
COLUMN
MATERIAL
TELL
US?
15
IS
THIS
C18
COLUMN
THE
RIGHT
CHOICE
FOR
MY
SAMPLE?
18
WHY
ARE
POLAR
SOLUTES
WELL
SEPARATED
WITH
ONE
CL
8
COLUMN
AND
HARDLY
AT
ALL
WITH
ANOTHER?
20
HOW
CAN
I
CLEAN
THE
RP
PHASE
QUICKLY?
23
HOW
BEST
DO
I
DEGAS
MY
MOBILE
PHASE?
24
METHANOL
OR
ACETONITRILE?
26
THE
PH
OF
THE
MOBILE
PHASE
TO
TOO
HIGH/TOO
LOW
-
WHAT
CAN
I
DO?
28
WHAT
IS
THE
RIGHT
IONIC
STRENGTH
OF
THE
BUFFER?
29
HOW
TO
MAKE
SENSE
OF
THE
DEAD
VOLUME
OF
AN
ISOCRATIC
APPARATUS?
31
PRODUCING
A
GRADIENT
CHROMATOGRAM
-
INFLUENCE
OF
INSTRUMENTATION
34
DOES
THE
PUMP
WORK
CORRECTLY,
PRECISELY
OR
ACCURATELY?
36
HOW
TO
TEST
AN
HPLC
INSTRUMENT
AND
ITS
MODULES?
39
INJECTION
OF
SOLUTES
AS
AQUEOUS
SOLUTIONS
41
WHAT
IS
THE
LARGEST
TOLERABLE
INJECTION
VOLUME?
43
HOW
CRITICAL
ARE
TEMPERATURE
CHANGES?
PART
I
45
HOW
CRITICAL
ARE
TEMPERATURE
CHANGES?
PART
II
47
HOW
TO
CHOOSE
HPLC
EQUIPMENT
AND
A
SUPPLIER?
53
IS
THE
CURRENT
METHOD
A
ROBUST
ONE?
57
PROBLEMS
AND
THEIR
SOLUTIONS
61
SAMPLE
PREPARATION
-
HOW
CRITICAL
ARE
WHICH
MISTAKES?
61
FLUSHING
OF
AN
HPLC
EQUIPMENT
63
DIRT
IN
THE
UV
DETECTION
CELL
65
THE
LAMP
IS
NEW
-
WHAT
HAPPENED
TO
THE
PEAK?
67
TIP
NO.
23
24
25
26
27
28
29
30
31
32
33
34
4.
35
36
37
38
39
40
41
42
43
44
45
ABOUT
5.
5.1
5.1.1
5.1.2
5.1.3
5.1.4
5.2
5.2.1
5.2.2
XIV
WHAT
ARE
THE
CAUSES
OF
PRESSURE
CHANGES
OR
DEVIATIONS?
69
IS
THE
RIGHT
OR
THE
LEFT
PUMP
HEAD
DEFECTIVE?
71
BASELINE
NOISE
AND
DAMPING
72
THE
RETENTION
TIMES
INCREASE
-
IS
IT
THE
PUMP
OR THE
MOBILE
PHASE?
75
WHICH
BUFFER
IS
RIGHT
FOR
WHICH
PH?
77
AN
INTERESTING
ALTERNATIVE
FOR
THE
SEPARATION
OF
ACIDS
AND
BASES
WITH
A
BUFFER
(FOR
A
DETAILED
DISCUSSION
ON
THE
SEPARATION
OF
ACIDS
AND
BASES
SEE
CHAPTER
5)
78
WHAT
CAN
BE
THE
REASONS
FOR
A
CHANGE
IN
RETENTION
TIMES?
81
I
USE
UP
A
LOT
OF
RP
COLUMNS;
WHAT
SHOULD
I
DO?
83
WHY
DOES
MY
NORMAL-PHASE
SYSTEM
NOT
WORK
ANY
MORE?
85
CHEMICAL
TAILING
AT
THE
PRESENCE
OF
METAL
IONS
87
HOW
TO
AVOID
MEMORY
EFFECTS?
91
HOW
DO
THE
DEFAULT
VALUES
ON
MY
PC
AFFECT
THE
RESOLUTION?
93
TIPS
TO
OPTIMIZE
THE
SEPARATION
99
WHICH
IS
THE
RIGHT
INJECTION
TECHNIQUE
TO
GET
SHARPER
PEAKS?
99
MY
PEAKS
APPEAR
TOO
EARLY
-
HOW
CAN
I
MOVE
THEM
IN
AN
RP
SYSTEM
TO
LATER
RETENTION
TIMES?
101
HOW
CAN
I
INCREASE
THE
PLATE
NUMBER?
103
LIMIT
OF
DETECTION:
HOW
CAN
I
SEE
MORE?
105
HOW
CAN
I
SPEED
UP
A
SEPARATION?
107
HOW
CAN
I
OPTIMIZE
A
SEPARATION?
108
DEAD
VOLUME,
CAPACITY
FACTOR,
SELECTIVITY
-
HOW
CAN
I
USE
THEM
IN
EVERYDAY
LIFE?
ILL
WHICH
FLOW
IS
OPTIMAL
FOR
ME?
113
HOW
CAN
I
OPTIMIZE
A
GRADIENT
ELUTION?
115
SEPARATION
OF
IONIC
SOLUTES:
WHAT
WORKS
OUT
BEST
-
ENDCAPPED
PHASES,
INERT
PHASES,
PHOSPHATE
BUFFER
OR
ION
PAIRING
REAGENTS?
PART
I
118
SEPARATION
OF
IONIC
SOLUTES:
WHAT
WORKS
OUT
BEST
-
ENDCAPPED
PHASES,
INERT
PHASES,
PHOSPHATE
BUFFER
OR
ION-PAIRING
REAGENTS?
PART
II
(SEE
ALSO
CHAPTER
5
)
120
IONIZABLE
SOLUTES,
SUN,
AND
LECTURES
IN
THE
AFTERNOON
122
RETENTION
OF
IONIZABLE
COMPONENTS
IN
REVERSED-PHASE
HPLC
123
INTRODUCTION
123
HISTORY
123
ANALYTE
IONIZATION
124
IONIZATION
AND
HPLC
RETENTION
IN
REVERSED-PHASE
HPLC
124
FACTORS
THAT
SHOULD
BE
CONSIDERED
PRIOR
TO
METHOD
DEVELOPMENT
127
METHOD
DEVELOPMENT
127
GENERAL
APPROACH
TO
METHOD
DEVELOPMENT
127
BASIC
METHOD
DEVELOPMENT
130
5.2.3
METHOD
OPTIMIZATION
134
5.2.4
CONCLUSION
137
5.3
METHOD
FINE
TUNING
137
5.3.1
SOLVATION
OF
THE
ACIDS
137
5.3.2
IONIZATION
OF
THE
ACIDS
138
5.3.3
CHAOTROPIC
EFFECTS
139
5.4
CONCLUDING
REMARKS
157
5.5
REFERENCES
TO
CHAPTER
5
158
6.
APPENDIX
159
6.1
SOME
CHROMATOGRAPHIC
AND
RELATED
ABBREVIATIONS
(SELECTION)
160
6.2
IUPAC
RECOMMENDATIONS
FOR
SYMBOLS
IN
CHROMATOGRAPHY
(A
SELECTION)
163
6.3
SOLVENT
MIXTURES
OF
EQUAL
ELUTION
STRENGTH
FOR
REVERSED
PHASE
CHROMATOGRAPHY
(ACCORDING
TO
L.
SNYDER)
164
6.4
UV
ABSORPTION
BANDS
AND
MOLAR
EXTINCTION
COEFFICIENTS
OF
SOME
TYPICAL
CHROMOPHORES
165
6.5
LIST
OF
TABLES
166
6.6
HPLC
TEXTBOOKS
168
6.7
TRENDS
IN
HPLC
169
6.7.1
HPLC
IN
ROUTINE
ANALYSIS
173
6.7.2
HPLC
IN
A
RESEARCH
ENVIRONMENT
173
INDEX
177
XV |
| any_adam_object | 1 |
| author | Kromidas, Stavros 1954- |
| author_GND | (DE-588)122124685 |
| author_facet | Kromidas, Stavros 1954- |
| author_role | aut |
| author_sort | Kromidas, Stavros 1954- |
| author_variant | s k sk |
| building | Verbundindex |
| bvnumber | BV013203617 |
| callnumber-first | Q - Science |
| callnumber-label | QD79 |
| callnumber-raw | QD79.C454 QP519.9.H53 |
| callnumber-search | QD79.C454 QP519.9.H53 |
| callnumber-sort | QD 279 C454 |
| callnumber-subject | QD - Chemistry |
| classification_rvk | VG 7500 VG 7501 |
| classification_tum | CHE 232f |
| ctrlnum | (OCoLC)43186299 (DE-599)BVBBV013203617 |
| dewey-full | 543.84 543.0894 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 543 - Analytical chemistry |
| dewey-raw | 543.84 543.0894 |
| dewey-search | 543.84 543.0894 |
| dewey-sort | 3543.84 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Chemie |
| format | Book |
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| id | DE-604.BV013203617 |
| illustrated | Illustrated |
| indexdate | 2024-08-20T00:39:46Z |
| institution | BVB |
| isbn | 3527298428 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008995907 |
| oclc_num | 43186299 |
| open_access_boolean | |
| owner | DE-29T DE-20 DE-355 DE-BY-UBR DE-703 DE-19 DE-BY-UBM DE-M49 DE-BY-TUM DE-Er8 DE-526 DE-634 DE-83 DE-11 DE-188 |
| owner_facet | DE-29T DE-20 DE-355 DE-BY-UBR DE-703 DE-19 DE-BY-UBM DE-M49 DE-BY-TUM DE-Er8 DE-526 DE-634 DE-83 DE-11 DE-188 |
| physical | XV, 178 S. graph. Darst. |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| publisher | Wiley-VCH |
| record_format | marc |
| spelling | Kromidas, Stavros 1954- Verfasser (DE-588)122124685 aut HPLC-Tips Practical problem solving in HPLC Stavros Kromidas Weinheim [u.a.] Wiley-VCH 2000 XV, 178 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier HPLC gtt Probleemoplossing gtt High performance liquid chromatography Phase partition HPLC (DE-588)4072641-1 gnd rswk-swf Fehlersuche (DE-588)4016615-6 gnd rswk-swf HPLC (DE-588)4072641-1 s Fehlersuche (DE-588)4016615-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008995907&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Kromidas, Stavros 1954- Practical problem solving in HPLC HPLC gtt Probleemoplossing gtt High performance liquid chromatography Phase partition HPLC (DE-588)4072641-1 gnd Fehlersuche (DE-588)4016615-6 gnd |
| subject_GND | (DE-588)4072641-1 (DE-588)4016615-6 |
| title | Practical problem solving in HPLC |
| title_alt | HPLC-Tips |
| title_auth | Practical problem solving in HPLC |
| title_exact_search | Practical problem solving in HPLC |
| title_full | Practical problem solving in HPLC Stavros Kromidas |
| title_fullStr | Practical problem solving in HPLC Stavros Kromidas |
| title_full_unstemmed | Practical problem solving in HPLC Stavros Kromidas |
| title_short | Practical problem solving in HPLC |
| title_sort | practical problem solving in hplc |
| topic | HPLC gtt Probleemoplossing gtt High performance liquid chromatography Phase partition HPLC (DE-588)4072641-1 gnd Fehlersuche (DE-588)4016615-6 gnd |
| topic_facet | HPLC Probleemoplossing High performance liquid chromatography Phase partition Fehlersuche |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008995907&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT kromidasstavros hplctips AT kromidasstavros practicalproblemsolvinginhplc |