Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
1999
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 240 S. graph. Darst. : 21 cm |
Internformat
MARC
| LEADER | 00000nam a22000001c 4500 | ||
|---|---|---|---|
| 001 | BV013131109 | ||
| 003 | DE-604 | ||
| 007 | t | ||
| 008 | 000425s1999 gw d||| m||| 00||| eng d | ||
| 016 | 7 | |a 958595488 |2 DE-101 | |
| 035 | |a (OCoLC)51832977 | ||
| 035 | |a (DE-599)BVBBV013131109 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c DE | ||
| 049 | |a DE-355 |a DE-83 |a DE-188 | ||
| 100 | 1 | |a Sultana, Razia |d 1970- |e Verfasser |0 (DE-588)121863395 |4 aut | |
| 245 | 1 | 0 | |a Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida |c vorgelegt von Razia Sultana |
| 264 | 1 | |c 1999 | |
| 300 | |a 240 S. |b graph. Darst. : 21 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Köln, Univ., Diss., 1999 | ||
| 650 | 0 | 7 | |a Pseudomonas putida |0 (DE-588)4123998-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Pyoverdine |0 (DE-588)4285270-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Isolierung |g Chemie |0 (DE-588)4122223-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Strukturaufklärung |0 (DE-588)4183788-5 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Pseudomonas putida |0 (DE-588)4123998-2 |D s |
| 689 | 0 | 1 | |a Pyoverdine |0 (DE-588)4285270-5 |D s |
| 689 | 0 | 2 | |a Isolierung |g Chemie |0 (DE-588)4122223-4 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Pseudomonas putida |0 (DE-588)4123998-2 |D s |
| 689 | 1 | 1 | |a Pyoverdine |0 (DE-588)4285270-5 |D s |
| 689 | 1 | 2 | |a Strukturaufklärung |0 (DE-588)4183788-5 |D s |
| 689 | 1 | |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008945901&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008945901 | |
Datensatz im Suchindex
| _version_ | 1807772930899181568 |
|---|---|
| adam_text |
CONTENT
1
INTRODUCTION
1
1.1
PSEUDOMONADS
1
1.2
SIDEROPHORES
FROM
FLUORESCENT
PSEUDOMONADS
1
1.2.1
PYOVERDINES
1
1.2.2
ISOPYOVERDINES
3
1.2.3
STRUCTURALLY
RELATED
SIDEROPHORES
3
1.3
BIOSYNTHESIS
OFPYOVERDINES
AND
ISOPYOVERDINES
5
1.4
PYOVERDINE
MEDIATED
IRON
UPTAKE
8
1.5
PRACTICAL
IMPORTANCE
OF
PYOVERDINES
9
1.5.1
PLANT-RELATED
BIOCONTROL
9
1.5.2
HUMAN
PATHOGENICITY
9
1.6
PURPOSE
OF
WORK
11
1.6.1
ORIGIN,
TAXONOMY
AND
IDENTIFICATION
OF
PSEUDOMONAS
PUTIDA
11
CFML
90-51,
CFML
95-275,
CFML
90-44
AND
CFML
90-33
PART
A
17
2
CULTIVATION
AND
ISOLATION
OF
PYOVERDINES
FROM
18
PSEUDOMONAS
PUTIDA
CFML
90-51
2.1
CULTIVATION
OF
BACTERIA
18
2.2
ISOLATION
OF
FERRI-PP
CFML
90-51
AND
FERRI-PP
CFML
90-51A
18
2.3
CHROMATOGRAPHIC
SEPARATION
19
2.3.1
GEL
FILTRATION
ON
BIO-GEL
19
2.3.2
SEPARATION
BY
ION-EXCHANGE
CHROMATOGRAPHY
19
2.3.3
SEPARATION
OF
FRACTION
FE-4-3
BY
PREPARATIVE
RP-HPLC
19
2.3.4
DECOMPLEXATION
22
3
CHARACTERIZATION
OF
PYOVERDINE
PP
CFML
90-51
25
3.1
UV/VIS-SPECTROSCOPY
25
3.2
MOLECULAR
MASS
DETERMINATION
BY
PI-FAB-MASS
SPECTROMETRY
27
3.3
ANALYSIS
AND
ABSOLUTE
CONFIGURATION
OF
THE
AMINO
ACIDS
29
3.4
DETERMINATION
OF
FREE
AMINO
GROUPS
31
3.5
SUMMARY
OFTHE
CHARACTERIZATION
OF
PYOVERDINE
PP
CFML
90-51
33
4
NMR-SPECTROSCOPY
34
4.1
INTRODUCTION
34
4.2
NMR-SPECTROSCOPIC
INVESTIGATIONS
OFPYOVERDINE
PPCFML
90-51
35
4.2.1
ASSIGNMENT
OF
THE'H-NMR
RESONANCES
35
4.2.2
ASSIGNMENT
OF
THE
13
C-NMR
RESONANCES
45
4.3
DISCUSSION
OF
THE
NMR-SPECTROSCOPIC
RESULTS
53
4.4
NMR-SPECTROSCOPIC
SEQUENCE
ANALYSIS
OF
THE
PYOVERDINE
55
PPCFML
90-51
4.4.1
SEQUENCE
ANALYSIS
BY
ROESY
AND
NOESY-EXPERIMENTS
55
4.4.2
SEQUENCE
ANALYSIS
BY
HMBC-EXPERIMENT
58
5
ESI-MASS
SPECTROMETRY
OF
PYOVERDINE
PP
CFML
90-51
61
5.1
INTRODUCTION
61
5.1.1
COMMON
NOMENCLATURE
FOR
SEQUENCE
IONS
IN
MASS
SPECTRA
62
OF
PEPTIDES
5.2
ION
TRAP
OCTAPOLE
CID-MASS
SPECTROMETRIC
INVESTIGATIONS
OF
63
PYOVERDINE
PP
CFML
90-51
5.3
ION
TRAP
CID
MASS-SPECTROMETRIC
INVESTIGATIONS
OF
PYOVERDINE
67
PPCFML
90-51
5.4
SUMMARY
OF
THE
ESI-MASS
SPECTROMETRIC
SEQUENCE
ANALYSIS
69
5.5
SEQUENCING
THE
PEPTIDE
CHAIN
OF
PYOVERDINE
PP
CFML
90-51
BY
71
PARTIAL
HYDROLYSIS
AND
FAB-MS
6
DETERMINATION
OF
THE
ABSOLUTE
CONFIGURATION
OF
PYOVERDINE
73
PP
CFML
90-51
CHROMOPHORE
6.1
ISOLATION
OF
THE
5-HYDROXYQUINOLINE
CHROMOPHORE
74
6.2
IDENTIFICATION
AND
DETERMINATION
OF
THE
ABSOLUTE
CONFIGURATION
OF
THE
74
CHROMOPHORE
PART
B
77
7
ISOLATION
OF
PYOVERDINE
PP
CFML
95-275
FROM
PSEUDOMONAS
78
PUTIDA
CFML
95-275
7.1
CHROMATOGRAPHIC
SEPARATION
78
7.1.1
GEL
FILTRATION
ON
BIO-GEL
78
7.1.2
SEPARATION
BY
ION-EXCHANGE
CHROMATOGRAPHY
78
7.1.3
DECOMPLEXATION
79
8
CHARACTERIZATION
OF
PYOVERDINE
PP
CFML
95-275
82
8.1
UV/VIS-SPECTROSCOPY
82
8.2
MOLECULAR
MASS
DETERMINATION
BY
PI-FAB-MASS
SPECTROMETRY
84
8.3
ANALYSIS
AND
ABSOLUTE
CONFIGURATION
OF
THE
AMINO
ACIDS
86
8.4
DETERMINATION
OF
FREE
AMINO
GROUPS
87
8.5
SUMMARY
OF
THE
CHARACTERIZATION
OF
PYOVERDINE
PP
CFML
95-275
88
9
NMR-SPECTROSCOPIC
INVESTIGATIONS
OF
PYOVERDINE
89
PP
CFML
95-275
9.1
ASSIGNMENT
OF
THE
'
H-NMR
RESONANCES
89
9.2
ASSIGNMENT
OF
THE
L3
C-NMR
RESONANCES
97
9.3
DISCUSSION
OF
THE
NMR-SPECTROSCOPIC
RESULTS
103
9.4
NMR-SPECTROSCOPIC
SEQUENCE
ANALYSIS
OF
PYOVERDINE
PP
CFML
95-275
106
9.4.1
SEQUENCE
ANALYSIS
BY
ROESY
AND
NOESY-EXPERIMENTS
106
9.4.2
SEQUENCE
ANALYSIS
BY
HMBC-EXPERIMENT
110
10
ESI-MASS
SPECTROMETRY
OF
PYOVERDINE
PP
CFML
95-275
114
10.1
ION
TRAP
OCTAPOLE
CID-MASS
SPECTROMETRIC
INVESTIGATIONS
OF
PYOVERDINE
114
PP
CFML
95-275
10.2
ION
TRAP
CID-MASS
SPECTROMETRIC
INVESTIGATIONS
OF
PYOVERDINE
115
PP
CFML
95-275
10.3
SUMMARY
OF
THE
ESI-MASS
SPECTROMETRIC
SEQUENCE
ANALYSIS
119
10.4
SEQUENCING
THE
PEPTIDE
CHAIN
OFPP
CFML
95-275
BY
121
PARTIAL
HYDROLYSIS
AND
FAB-MS
PART
C
123
11
ISOLATION
OF
ISOPYOVERDINE
PP
CFML
90-44
FROM
PSEUDOMONAS
124
PUTIDA
CFML
90-44
11.1
CHROMATOGRAPHIC
SEPARATION
124
11.1.1
GEL
FILTRATION
ON
BIO-GEL
124
11.1.2
SEPARATION
BY
ION-EXCHANGE
CHROMATOGRAPHY
124
11.1.3
DECOMPLEXATION
125
12
CHARACTERIZATION
OF
ISOPYOVERDINE
PP
CFML
90-44
128
12,1
UV/VIS-SPECTROSCOPY
128
12.2
MOLECULAR
MASS
DETERMINATION
BY
PI-FAB-MASS
SPECTROMETRY
130
12.3
ANALYSIS
AND
ABSOLUTE
CONFIGURATION
OF
THE
AMINO
ACIDS
131
12.4
DETERMINATION
OF
FREE
AMINO
GROUPS
132
12.5
SUMMARY
OF
THE
CHARACTERIZATION
OF
PYOVERDINE
PP
CFML
95-275
133
13
NMR-SPECTROSCOPIC
INVESTIGATIONS
OF
ISOPYOVERDINE
134
PP
CFML
90-44
13.1
ASSIGNMENT
OF
THE
'
H-NMR
RESONANCES
134
13.2
ASSIGNMENT
OF
THE
N
C-NMR
RESONANCES
141
13.3
DISCUSSION
OF
THE
NMR-SPECTROSCOPIC
RESULTS
147
13.4
NMR-SPECTROSCOPIC
SEQUENCE
ANALYSIS
OF
ISOPYOVERDINE
149
PP
CFML
90-44
13.4.1
SEQUENCE
ANALYSIS
BY
ROESY-AND
NOES
Y-EXPERIMENTS
149
13.4.2
SEQUENCE
ANALYSIS
BY
HMBC-EXPERIMENT
151
14
ESI-MASS
SPECTROMETRY
OF
ISOPYOVERDINE
PP
CFML
90-44
154
14.1
ION
TRAP
OCTAPOLE
CID-MASS
SPECTROMETRIC
INVESTIGATIONS
OF
154
ISOPYOVERDINE
PP
CFML
90-44
14.2
ION
TRAP
CID-MASS
SPECTROMETRIC
INVESTIGATIONS
OF
ISOPYOVERDINE
157
PP
CFML
90-44
14.3
SUMMARY
OF
THE
ESI-MASS
SPECTROMETRIC
SEQUENCE
ANALYSIS
158
PART
D
160
15
ISOLATION
OF
ISOPYOVERDINE
PP
CFML
90-33
FROM
PSEUDOMONAS
161
PUTIDA
CFML
90-33
15.1
CHROMATOGRAPHIC
SEPARATION
161
15.1.1
GEL
FILTRATION
ON
BIO-GEL
161
15.1.2
SEPARATION
BY
ION-EXCHANGE
CHROMATOGRAPHY
161
15.1.3
DECOMPLEXATION
162
16
CHARACTERIZATION
OF
ISOPYOVERDINE
PP
CFML
90-33
165
16.1
UV/VIS-SPECTROSCOPY
167
16.2
MOLECULAR
MASS
DETERMINATION
BY
PI-FAB-MASS
SPECTROMETRY
167
16.3
ANALYSIS
AND
ABSOLUTE
CONFIGURATION
OF
THE
AMINO
ACIDS
168
16.4
DETERMINATION
OF
FREE
AMINO
GROUPS
169
16.5
SUMMARY
OF
THE
CHARACTERIZATION
OF
ISOPYOVERDINE
PP
CFML
90-33
170
17
NMR-SPECTROSCOPIC
INVESTIGATIONS
OF
ISOPYOVERDINE
171
PP
CFML
90-33
17.1
ASSIGNMENT
OF
THE'H-NMR
RESONANCES
171
17.2
ASSIGNMENT
OF
THE
N
C-NMR
RESONANCES
178
17.3
DISCUSSION
OF
THE
NMR-SPECTROSCOPIC
RESULTS
184
17.4
NMR-SPECTROSCOPIC
SEQUENCE
ANALYSIS
OF
ISOPYOVERDINE
186
PP
CFML
90-33
17.4.1
SEQUENCE
ANALYSIS
BY
ROESY
AND
NOESY-EXPERIMENTS
186
17.4.2
SEQUENCE
ANALYSIS
BY
HMBC-EXPERIMENT
189
18
ESI-MASS
SPECTROMETRY
OF
THE
ISOPYOVERDINE
PP
CFML
90-33
192
18.1
ION
TRAP
OCTAPOLE
CID-MASS
SPECTROMETRIC
INVESTIGATIONS
OF
192
ISOPYOVERDINE
PP
CFML
90-33
18.2
ION
TRAP
CID-MASS
SPECTROMETRIC
INVESTIGATIONS
OF
ISOPYOVERDINE
194
PP
CFML
90-33
18.3
SUMMARY
OF
THE
ESI-MASS
SPECTROMETRIC
SEQUENCE
ANALYSIS
195
18.4
SEQUENCING
THE
PEPTIDE
CHAIN
OF
ISOPYOVERDINE
PP
CFML
90-33
BY
197
PARTIAL
HYDROLYSIS
AND
FAB-MS
19
DETERMINATION
OF
THE
ABSOLUTE
CONFIGURATION
OF
ISOPYOVERDINE
199
PP
CFML
90-33
CHROMOPHORE
19.1
ISOLATION
OF
THE
5-HYDROXYQUINOLINE
CHROMOPHORE
200
20
DEUTSCHE
ZUSAMMENFASSUNG
201
21
EXPERIMENTAL
210
21.1
INSTRUMENTS
AND
MATERIALS
210
21.1.1
ANALYTICAL
AND
PREPARATIVE
SEPARATION
210
21.1.2
INSTRUMENTS
FOR
SPECTROSCOPY
211
21.1.3
OTHER
INSTRUMENTS
212
21.2
CHEMICALS
212
21.3
MICROBIOLOGICAL
WORK
213
21.3.1
CULTIVATION
OF
BACTERIA
213
21.4
ISOLATION
OF
SIDEROPHORES
213
21.4.1
ISOLATION
OFFERRI-PP
CFML
90-51
213
21.4.2
CHROMATOGRAPHIC
SEPARATIONS
214
21.4.2.1
CHROMATOGRAPHIC
SEPARATION
OF
THE
XAD-4
EXTRACT
OF
214
P.PUTIDA
CFML
90-51
21.4.2.2
CHROMATOGRAPHIC
SEPARATION
OF
THE
XAD-4
EXTRACT
OF
215
P.PUTIDA
CFML
95-275
21.4.2.3
CHROMATOGRAPHIC
SEPARATION
OF
THE
XAD-4
EXTRACT
OF
216
P.
PUTIDA
CFML
90-44
21.4.2.4
CHROMATOGRAPHIC
SEPARATION
OF
THE
XAD-4
EXTRACT
OF
217
P.PUTIDA
CFML
90-33
21.4.2.5
PURITY-CHECK
BY
ANALYTICAL
RP-HPLC
218
21.5
DECOMPLEXATION
OF
FERRI-PYOVERDINES
AND
FERRI-ISOPYOVERDINES
218
21.6
AMINO
ACID
ANALYSIS
219
21.6.1
TOTAL
HYDROLYSIS
AND
TAP-DERIVATIZATION
219
21.7
GAS
CHROMATOGRAPHY
219
21.8
PARTIAL
HYDROLYSIS
220
21.9
DETERMINATION
OF
FREE
AMINO
GROUPS
220
21.10
ISOLATION
OF
THE
MODIFIED
5-HYDROXYQUINOLINE
CHROMOPHORE
220
21.11
SPECTROSCOPY
221
21.11.1
UV/VIS-SPECTROSCOPY
221
21.11.2
MASS-SPECTROSCOPY
221
21.11.3
NMR-SPECTROSCOPY
221
21.11.4
CD-SPECTROSCOPY
224
22
BIBLIOGRAPHY
225
23
APPENDIX
240 |
| any_adam_object | 1 |
| author | Sultana, Razia 1970- |
| author_GND | (DE-588)121863395 |
| author_facet | Sultana, Razia 1970- |
| author_role | aut |
| author_sort | Sultana, Razia 1970- |
| author_variant | r s rs |
| building | Verbundindex |
| bvnumber | BV013131109 |
| ctrlnum | (OCoLC)51832977 (DE-599)BVBBV013131109 |
| format | Thesis Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV013131109 |
| illustrated | Illustrated |
| indexdate | 2024-08-19T00:16:51Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008945901 |
| oclc_num | 51832977 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-83 DE-188 |
| owner_facet | DE-355 DE-BY-UBR DE-83 DE-188 |
| physical | 240 S. graph. Darst. : 21 cm |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| record_format | marc |
| spelling | Sultana, Razia 1970- Verfasser (DE-588)121863395 aut Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida vorgelegt von Razia Sultana 1999 240 S. graph. Darst. : 21 cm txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1999 Pseudomonas putida (DE-588)4123998-2 gnd rswk-swf Pyoverdine (DE-588)4285270-5 gnd rswk-swf Isolierung Chemie (DE-588)4122223-4 gnd rswk-swf Strukturaufklärung (DE-588)4183788-5 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Pseudomonas putida (DE-588)4123998-2 s Pyoverdine (DE-588)4285270-5 s Isolierung Chemie (DE-588)4122223-4 s DE-604 Strukturaufklärung (DE-588)4183788-5 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008945901&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Sultana, Razia 1970- Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida Pseudomonas putida (DE-588)4123998-2 gnd Pyoverdine (DE-588)4285270-5 gnd Isolierung Chemie (DE-588)4122223-4 gnd Strukturaufklärung (DE-588)4183788-5 gnd |
| subject_GND | (DE-588)4123998-2 (DE-588)4285270-5 (DE-588)4122223-4 (DE-588)4183788-5 (DE-588)4113937-9 |
| title | Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida |
| title_auth | Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida |
| title_exact_search | Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida |
| title_full | Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida vorgelegt von Razia Sultana |
| title_fullStr | Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida vorgelegt von Razia Sultana |
| title_full_unstemmed | Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida vorgelegt von Razia Sultana |
| title_short | Isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of Pseudomonas putida |
| title_sort | isolation and structure elucidation of new pyoverdines and isopyoverdines from four different strains of pseudomonas putida |
| topic | Pseudomonas putida (DE-588)4123998-2 gnd Pyoverdine (DE-588)4285270-5 gnd Isolierung Chemie (DE-588)4122223-4 gnd Strukturaufklärung (DE-588)4183788-5 gnd |
| topic_facet | Pseudomonas putida Pyoverdine Isolierung Chemie Strukturaufklärung Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008945901&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT sultanarazia isolationandstructureelucidationofnewpyoverdinesandisopyoverdinesfromfourdifferentstrainsofpseudomonasputida |