A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Mikrofilm Buch |
| Sprache: | English |
| Veröffentlicht: |
1999
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| Ausgabe: | [Mikrofiche-Ausg.] |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Kaiserslautern, Univ., Diss., 1999. - Mikrofiche-Ausg.: 2 Mikrofiches : 24x |
| Beschreibung: | [11], 95, [5] Bl. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries |c von Taseer A. Bashir |
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Datensatz im Suchindex
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| adam_text |
LIST OF CONTENTS
ACKNOWLEDGMENTS. A
SUMMARY. B
CHAPTER 1.1
INTRODUCTION. 1
1.1 IMPORTANT CONCEPTS AND SYSTEMS OF CLASSIFICATION.1
1.1.1 NOMINALISM. 1
1.1.2 PHENETICS. 2
1.1.3 ARTIFICIAL.2
1.1.4 PHYLOGENETIC.2
1.2 STREPTOMYCES AND DETERMINATION OF RELATEDNESS AMONG STRAINS.3
1.3 CURRENT PHENETIC CLASSIFICATION AND DETERMINATION OF HOMOLOGY AMONG
STREPTOMYCES
STRAINS.6
1.4 MODEM TECHNIQUES AND STUDY OF HOMOLOGIES AMONG STREPTOMYCES.7
1.4.1 CHEMICAL CHARACTERIZATION OF THE CELL AS TAXONOMICAL TOOLS.7
1.4.1.1 COMPONENTS OF CELL WALL AS RELATEDNESS DETERMINANT MARKERS.8
1.4.1.2 UNIVERSAL PRESENCE OF CYTOPLASMIC MEMBRANE AND ITS CHEMICAL
DIVERSITY.8
1.4.2 CHEMICAL FINGERPRINTING AS THE ASSESSMENT OF OVERALL DEGREE OF
SIMILARITY.9
1.4.3 ONE DIMENSIONAL AND TWO DIMENSIONAL PROTEIN PATTERNS.10
1.5 GENOME AND ITS ROLE IN DETERMINATION OF RELATEDNESS.10
1.6 DNA METHODS IN THE DETERMINATION OF INTER-STRAIN AND INTER-SPECIES
DIVERSITY.11
1.7 STUDIES ON DNA-DNA RELATEDNESS OF STRAINS AND EFFORTS TO VERIFY THE
NUMERIC
PHENETIC CLASSIFICATION OF
STREPTOMYCES.15
1.8 THE BACKGROUND OF THE PRESENT PROJECT.19
1.9 AIMS OF THE PROJECT.22
CHAPTER 2. 23
MATERIALS AND METHODS.23
2.1 MATERIALS.23
2.1.1 STREPTOMYCES STRAINS.23
2.1.2 COSMIDS.23
2.1.2.1 COSMIDS FROM ORDERED COSMID LIBRARY OF S. COELICOTOR A3(2).23
2.1.2.2 COSMIDS FROM STREPTOMYCES LIVIDANS 66.24
2.1.3 BACTERIAL MEDIA.25
2.1.4 STOCK SOLUTIONS.25
2.1.5 OTHER MATERIALS.26
2.2 METHODS.27
2.2.1 CULTURE CONDITIONS AND STRAINS MAINTENANCE. 27
2.2.2 DNA MANIPULATION.28
2.2.2.1 ISOLATION OF PLASMID AND COSMID DNA.28
2.2.2.2 ISOLATION OF TOTAL STREPTOMYCES DNA.28
2.2.2.3 PHENOL CHLOROFORM EXTRACTION.29
2.2.2.4 RESTRICTION ENDONUCLEASE DIGESTIONS.29
2.2.2.5 AGAROSE GEL ELECTROPHORESIS.30
2.2.2.6 SOUTHERN BLOTTING.30
22,2.1 NON-RADIOACTIVE DNA LABELING WITH 11 -DIGOXYGININE-DUTP.31
2.2.2.8 DNA-DNA HYBRIDIZATION.32
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/958261857
2.2.2.9. COLOUR DETECTION. 32
2.2.2.10 CHEMILUMINISCENT DETECTION.33
2.3 THE EXPERIMENTAL PLAN.34
2.3.1 RFLP STUDIES EMPLOYING PSTL RESTRICTED CHROMOSOMAL DNA OF TEST
STRAINS.34
2.3.2 RFLP STUDIES EMPLOYING BAMHL RESTRICTED CHROMOSOMAL DNA OF TEST
STRAINS.34
2.3.3 DISTRIBUTION OF DAGA GENE IN THE CLUSTER 21. 34
2.3.4 FINGERPRINTING WITH COSMIDS FROM TERMINAL REGIONS OF
STREPYTOMYCES LIVIDANS 66 . 34
2.4 DATA ANALYSIS.34
2.4.1 DATA CONVERSION SCHEME.35
2.4.2 ANALYSIS OF INTER-STRAIN DIVERSITY.36
CHAPTER 3.37
RESULTS . 37
3.1 CHOICE OF STRAINS.37
3.2 CHOICE OF COSMIDS.38
3.3 RFLPS WITH PSTL RESTRICTED CHROMOSOMAL DNA.39
3.4 RFLPS WITH BAMHL RESTRICTED CHROMOSOMAL DNA.40
3.5 THE QUANTITATIVE ANALYSIS OF BANDING PATTERNS.46
3.6 ANALYSIS OF INTER-STRAIN DIVERSITY. 55
3.7 THE AGARASE REGION.55
3.8 USE OF RFLPS FOR FINGERPRINTING.58
3.8.1 COSMIDS SHOWING LOWER VARIABILITY.58
3.8.2 COSMIDS SHOWING HIGHER VARIABILITY.62
3.8.3 BASIS OF FINGERPRINTING.63
CHAPTER 4.78
DISCUSSION. 78
REFERENCES.85
ANNEX-1
CURRICULUM VITAE |
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| author | Bashir, Taseer A. 1962- |
| author_GND | (DE-588)121708713 |
| author_facet | Bashir, Taseer A. 1962- |
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| building | Verbundindex |
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| edition | [Mikrofiche-Ausg.] |
| format | Thesis Microfilm Book |
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| genre_facet | Hochschulschrift |
| id | DE-604.BV013130518 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T18:13:35Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008945562 |
| oclc_num | 664757168 |
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| owner | DE-12 DE-188 |
| owner_facet | DE-12 DE-188 |
| physical | [11], 95, [5] Bl. Ill., graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| record_format | marc |
| spelling | Bashir, Taseer A. 1962- Verfasser (DE-588)121708713 aut A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries von Taseer A. Bashir [Mikrofiche-Ausg.] 1999 [11], 95, [5] Bl. Ill., graph. Darst. txt rdacontent h rdamedia he rdacarrier Kaiserslautern, Univ., Diss., 1999. - Mikrofiche-Ausg.: 2 Mikrofiches : 24x Kaiserslautern, Univ., Diss., 1999 Genetische Variabilität (DE-588)4264352-1 gnd rswk-swf Cosmid (DE-588)4358989-3 gnd rswk-swf Streptomyces griseoruber (DE-588)4585603-5 gnd rswk-swf RFLP (DE-588)4238815-6 gnd rswk-swf Genbibliothek (DE-588)4236160-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Streptomyces griseoruber (DE-588)4585603-5 s Genetische Variabilität (DE-588)4264352-1 s Cosmid (DE-588)4358989-3 s Genbibliothek (DE-588)4236160-6 s RFLP (DE-588)4238815-6 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008945562&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Bashir, Taseer A. 1962- A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries Genetische Variabilität (DE-588)4264352-1 gnd Cosmid (DE-588)4358989-3 gnd Streptomyces griseoruber (DE-588)4585603-5 gnd RFLP (DE-588)4238815-6 gnd Genbibliothek (DE-588)4236160-6 gnd |
| subject_GND | (DE-588)4264352-1 (DE-588)4358989-3 (DE-588)4585603-5 (DE-588)4238815-6 (DE-588)4236160-6 (DE-588)4113937-9 |
| title | A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries |
| title_auth | A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries |
| title_exact_search | A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries |
| title_full | A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries von Taseer A. Bashir |
| title_fullStr | A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries von Taseer A. Bashir |
| title_full_unstemmed | A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries von Taseer A. Bashir |
| title_short | A study of the genetic variability within the streptomyces cluster 21 (S. griseoruber) using ordered cosmid libraries |
| title_sort | a study of the genetic variability within the streptomyces cluster 21 s griseoruber using ordered cosmid libraries |
| topic | Genetische Variabilität (DE-588)4264352-1 gnd Cosmid (DE-588)4358989-3 gnd Streptomyces griseoruber (DE-588)4585603-5 gnd RFLP (DE-588)4238815-6 gnd Genbibliothek (DE-588)4236160-6 gnd |
| topic_facet | Genetische Variabilität Cosmid Streptomyces griseoruber RFLP Genbibliothek Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008945562&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT bashirtaseera astudyofthegeneticvariabilitywithinthestreptomycescluster21sgriseoruberusingorderedcosmidlibraries |