Experimental design for cardiac gene therapy: rat heart transplantation models and gene transfer techniques
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Münster
Schüling
2000
|
| Schriftenreihe: | Schriftenreihe Herz-Thorax-Chirurgie
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: Münster (Westfalen), Univ., Habil.-Schr. |
| Beschreibung: | 118 S. Ill., graph. Darst. |
| ISBN: | 3930962632 |
Internformat
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Datensatz im Suchindex
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|---|---|
| adam_text | Table of Contents 7
1 INTRODUCTION 9
2 METHODS 13
2.1 Experimental animals 15
2.1.1 Experimental groups 15
2.2 Heterotopic heart transplantation in the rat 16
2.2.1 Traditional non volume loaded heterotopic heart transplantation model 16
2.2.2 Volume loaded transplantation model 17
2.2.3 Allogeneic transplantation of volume loaded vs. traditional
heterotopic heart transplantation. 22
2.3 Hemodynamic analysis of the volume loaded (VL) vs. traditional non volume
loaded heterotopic heart transplantation model (NL) in isografts. 23
2.3.1 Computer software supported data recording 23
2.3.2 Pressure and ECG recordings 24
2.3.3 Redirection of volume load to the VL in situ 25
2.3.4 In situ volume dependent pressure development of VL and NL 25
2.3.5 Quantification of volume load 25
2.3.5.1 Angiography to demonstrate volume loading of the graft 25
2.3.5.2 Oxygen saturation in the abdominal aorta of NL and VL
Transplantation model 26
2.3.5.3 Heart weight of NL and VL transplant model. 26
2.3.6 Isolated rat heart Langendorff perfusion system 26
2.3.6.1 Hemodynamic response to p stimulation 27
2.3.6.2 Statistical analysis 27
2.4 Gene transfer techniques 27
2.4.1 Virus vector systems 27
2.4.1.1 Adenovirus vectors 27
2.4.1.2 Adeno associated virus vectors 29
2.4.2 Direct intramyocardial injection 30
2.4.3 Coronary internal recirculation of virus vectors 31
2.4.4 Coronary external recirculation of virus vectors 34
2.5 Histological analysis 38
2.5.1 Harvesting specimens 38
2.5.2 Staining of p galactosidase gene expression 38
2.5.3 Scoring of graft rejection and accelerated graft atherosclerosis 39
2.5.4 Statistical analysis 39
3 RESULTS 41
3.1 Traditional non volume loaded (NL) and volume loaded (VL) heterotopic
rat heart transplantation models 43
3.1.1 Hemodynamic analysis of the volume loaded (VL) vs. unloaded
transplantation model (NL). 44
3.1.1.1 Pressure and ECG recordings in situ. 44
3.1.1.2 Increase of pre load for the NL and VL grafts 48
3.1.1.3 Isolated Langendorff perfusion of NL and VL transplanted hearts
after 1 and 2 weeks 51
3.1.1.4 Right to left shunt caused by VL 56
3.1.1.5 Angiography 56
3.1.2 Allogeneic heterotopic heart transplantation of the traditional non volume
loaded (NL) vs. volume loaded (VL) model. 59
8 Table of Contents
3.2 Myocardial gene transfer 61
3.2.1 Direct myocardial injection 62
3.2.2 Trans coronary injection 65
3.2.2.1 Internal vector recirculation 65
3.2.2.2 External recirculation 67
4 DISCUSSION 71
4.1 The volume loaded vs. unloaded heterotopic heart transplantation model 74
4.1.1 Characterization of the volume loaded heterotopic heart
transplantation model (VL) 76
4.1.2 Immunological influence of the volume loaded (VL) allogeneic
transplanted heart 80
4.2 Gene transfer in the heart 82
4.2.1 DNA Viral vectors 85
4.2.1.1 Adenovirus 85
4.2.1.2 Adeno associated virus 88
4.2.2 Gene transfer techniques 89
4.2.2.1 Direct injection 89
4.2.2.2 Coronary perfusion 91
5 CONCLUSION 95
6 ACKNOWLEDGEMENTS 99
7 REFERENCES 103
|
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| indexdate | 2024-07-09T18:39:26Z |
| institution | BVB |
| isbn | 3930962632 |
| language | English |
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| physical | 118 S. Ill., graph. Darst. |
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| spelling | Asfour, Boulos Verfasser aut Experimental design for cardiac gene therapy rat heart transplantation models and gene transfer techniques von Boulos Asfour Münster Schüling 2000 118 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Schriftenreihe Herz-Thorax-Chirurgie Zugl.: Münster (Westfalen), Univ., Habil.-Schr. Gentransfer (DE-588)4194102-0 gnd rswk-swf Virusvektor (DE-588)4232137-2 gnd rswk-swf Herztransplantation (DE-588)4201513-3 gnd rswk-swf Adenoviren (DE-588)4141412-3 gnd rswk-swf Tiermodell (DE-588)4140660-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Herztransplantation (DE-588)4201513-3 s Adenoviren (DE-588)4141412-3 s Virusvektor (DE-588)4232137-2 s Gentransfer (DE-588)4194102-0 s Tiermodell (DE-588)4140660-6 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008939687&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Asfour, Boulos Experimental design for cardiac gene therapy rat heart transplantation models and gene transfer techniques Gentransfer (DE-588)4194102-0 gnd Virusvektor (DE-588)4232137-2 gnd Herztransplantation (DE-588)4201513-3 gnd Adenoviren (DE-588)4141412-3 gnd Tiermodell (DE-588)4140660-6 gnd |
| subject_GND | (DE-588)4194102-0 (DE-588)4232137-2 (DE-588)4201513-3 (DE-588)4141412-3 (DE-588)4140660-6 (DE-588)4113937-9 |
| title | Experimental design for cardiac gene therapy rat heart transplantation models and gene transfer techniques |
| title_auth | Experimental design for cardiac gene therapy rat heart transplantation models and gene transfer techniques |
| title_exact_search | Experimental design for cardiac gene therapy rat heart transplantation models and gene transfer techniques |
| title_full | Experimental design for cardiac gene therapy rat heart transplantation models and gene transfer techniques von Boulos Asfour |
| title_fullStr | Experimental design for cardiac gene therapy rat heart transplantation models and gene transfer techniques von Boulos Asfour |
| title_full_unstemmed | Experimental design for cardiac gene therapy rat heart transplantation models and gene transfer techniques von Boulos Asfour |
| title_short | Experimental design for cardiac gene therapy |
| title_sort | experimental design for cardiac gene therapy rat heart transplantation models and gene transfer techniques |
| title_sub | rat heart transplantation models and gene transfer techniques |
| topic | Gentransfer (DE-588)4194102-0 gnd Virusvektor (DE-588)4232137-2 gnd Herztransplantation (DE-588)4201513-3 gnd Adenoviren (DE-588)4141412-3 gnd Tiermodell (DE-588)4140660-6 gnd |
| topic_facet | Gentransfer Virusvektor Herztransplantation Adenoviren Tiermodell Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008939687&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT asfourboulos experimentaldesignforcardiacgenetherapyrathearttransplantationmodelsandgenetransfertechniques |