Costimulatory function of CD44: acting in unison with the T cell receptor
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
2000
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2000. - Auch als CD-Rom |
| Beschreibung: | III, 123 Bl. Ill., graph. Darst. |
Internformat
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Datensatz im Suchindex
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CONTENTS
CONTENTS
1. INTRODUCTION.1
1.1 T CELL ACTIVATION.1
1.2 ACTIN CYTOSKELETON.7
1.3 LIPID MICRODOMAINS IN CELL SURFACE MEMBRANES.10
1.4 FORMS AND FUNCTIONS OF CD44.11
1.4.1 STRUCTURE OF CD44.11
1.4.2 FUNCTIONS OF CD44 IN THE IMMUNE SYSTEM.14
1.5 AIM OF THE STUDY.17
2. MATERIALS AND METHODS.18
2.1 MATERIALS.18
2.1.1 GENERAL CHEMICALS AND MATERIALS.18
2.1.2 INHIBITORS.19
2.1.3 RADIOCHEMICALS.20
2.1.4 EXPRESSION VECTORS.20
2.1.5 PEPTIDES.20
2.1.6 ANTIBODIES.21
2.1.7 CD44 RECEPTOR GLOBULIN.22
2.1.8 EXPERIMENTAL ANIMALS.22
2.1.9 CELL LINES.23
2.2 METHODS.24
2.2.1 CELL CULTURE.24
2.2.2 LYMPHOCYTE PREPARATION AND FRACTIONATION.24
2.2.3 STIMULATION OF CELLS.25
2.2.4 ANTIBODY PURIFICATION.26
2.2.5 PROLIFERATION ASSAY.27
2.2.6 CYTOKINE ELISA.27
2.2.7 IMMUNOFLUORESCENCE STAINING FOR FLOW CYTOMETRY.28
I
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/959889507
CONTENTS
2.2.8 DETERMINATION OF APOPTOSIS.29
2.2.9 BIOSYNTHETIC LABELING OF CELLULAR PROTEINS.30
2.2.10 IMMUNOPRECIPITATION.30
2.2.11 IMMUNE COMPLEX KINASE ASSAY AND REPRECIPITATION.31
2.2.12 PURIFICATION OF GEM FRACTIONS.32
2.2.13 SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE).32
2.2.14 WESTERN BLOTTING. 33
2.2.15 DOT-BLOT IMMUNOASSAY.34
2.2.16 IMMUNOFLUORESCENCE MICROSCOPY.35
2.2.17 TRANSIENT TRANSFECTION.36
3.
RESULTS. 37
3.1 INHIBITION OF IL-2 SECRETION BY A CD44 RECEPTOR GLOBULIN AND
INDUCTION OF T CELL
PROLIFERATION BY CROSS-LINKING OF CD44.37
3.2 INDUCTION OF APOPTOSIS BY CROSS-LINKING OF CD44.46
3.3 CD44 IS COSTIMULATORY FOR CD3-INDUCED SIGNAL TRANSDUCTION EVENTS. 54
3.3.1 ACTIVATION OF BOTH ERK AND JNK PATHWAYS AFTER COLIGATION OF CD44
WITH CD3.55
3.3.2 CROSS-LINKING OF CD44 ENHANCES EARLY CD3-INDUCED SIGNALING
EVENTS.56
3.4 CD44 IS ASSOCIATED WITH SRC-FAMILY PROTEIN TYROSINE KINASES.64
3.5 REDISTRIBUTION OF SRC FAMILY KINASES INTO GLYCOLIPID-ENRICHED
MICRODOMAINS
(GEMS) UPON CROSS-LINKING OF CD44.67
3.6 CD44 ENGAGEMENT INDUCES CYTOSKELETAL REARRANGEMENTS IN T CELLS.71
4. DISCUSSION.81
4.1 COSTIMULATORY FUNCTION OF CD44 IN T CELL PROLIFERATION AND
APOPTOSIS.82
4.2 COSTIMULATORY FUNCTION OF CD44 ON CD3-INDUCED SIGNAL TRANSDUCTION.84
4.2.1 COSTIMULATORY FUNCTION OF CD44 ON CD3-INDUCED ERK AND JNK
ACTIVATION.84
4.2.2 COSTIMULATORY ACTIVITY OF CD44 ACTS ON TCR-PROXIMAL SIGNALING
EVENTS.85
4.3 MECHANISM OF CD44-MEDIATED COSTIMULATORY ACTIVITY.89
4.3.1 CD44 FUNCTIONING AS AN ADHESION MOLECULE.89
4.3.2 CD44 CONTRIBUTES TO CYTOSKELETAL REARRANGEMENTS.90
4.3.3 RECRUITMENT OF SRC-FAMILY PROTEIN TYROSINE KINASES BY CD44.94
4.3.4 COSTIMULATORY FUNCTION OF CD44 BY REORGANIZATION OF MEMBRANE
MICRODOMAINS.95
II
CONTENTS
5. SUMMARY.98
6. REFERENCES-- 101
7. ABBREVIATIONS. 122
III |
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| illustrated | Illustrated |
| indexdate | 2024-10-09T18:10:51Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008927993 |
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| physical | III, 123 Bl. Ill., graph. Darst. |
| publishDate | 2000 |
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| spelling | Föger, Niko 1968- Verfasser (DE-588)122383346 aut Costimulatory function of CD44 acting in unison with the T cell receptor von Niko Föger 2000 III, 123 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zsfassung in dt. Sprache. - Würzburg, Univ., Diss., 2000. - Auch als CD-Rom Aktivierung Physiologie (DE-588)4243777-5 gnd rswk-swf T-Lymphozyt (DE-588)4127387-4 gnd rswk-swf T-Lymphozyten-Rezeptor (DE-588)4265934-6 gnd rswk-swf Antigen CD44 (DE-588)4359649-6 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Antigen CD44 (DE-588)4359649-6 s T-Lymphozyten-Rezeptor (DE-588)4265934-6 s T-Lymphozyt (DE-588)4127387-4 s Aktivierung Physiologie (DE-588)4243777-5 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008927993&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Föger, Niko 1968- Costimulatory function of CD44 acting in unison with the T cell receptor Aktivierung Physiologie (DE-588)4243777-5 gnd T-Lymphozyt (DE-588)4127387-4 gnd T-Lymphozyten-Rezeptor (DE-588)4265934-6 gnd Antigen CD44 (DE-588)4359649-6 gnd |
| subject_GND | (DE-588)4243777-5 (DE-588)4127387-4 (DE-588)4265934-6 (DE-588)4359649-6 (DE-588)4113937-9 |
| title | Costimulatory function of CD44 acting in unison with the T cell receptor |
| title_auth | Costimulatory function of CD44 acting in unison with the T cell receptor |
| title_exact_search | Costimulatory function of CD44 acting in unison with the T cell receptor |
| title_full | Costimulatory function of CD44 acting in unison with the T cell receptor von Niko Föger |
| title_fullStr | Costimulatory function of CD44 acting in unison with the T cell receptor von Niko Föger |
| title_full_unstemmed | Costimulatory function of CD44 acting in unison with the T cell receptor von Niko Föger |
| title_short | Costimulatory function of CD44 |
| title_sort | costimulatory function of cd44 acting in unison with the t cell receptor |
| title_sub | acting in unison with the T cell receptor |
| topic | Aktivierung Physiologie (DE-588)4243777-5 gnd T-Lymphozyt (DE-588)4127387-4 gnd T-Lymphozyten-Rezeptor (DE-588)4265934-6 gnd Antigen CD44 (DE-588)4359649-6 gnd |
| topic_facet | Aktivierung Physiologie T-Lymphozyt T-Lymphozyten-Rezeptor Antigen CD44 Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008927993&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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