Apoptosis regulation by Raf-1 and Bcl-2:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English German |
| Veröffentlicht: |
1999
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Würzburg, Univ., Diss., 2000. - Zsfassung in dt. Sprache |
| Beschreibung: | 84 Bl. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV013098200 | ||
| 003 | DE-604 | ||
| 005 | 20020228 | ||
| 007 | t | ||
| 008 | 000406s1999 ad|| m||| 00||| eng d | ||
| 016 | 7 | |a 959863656 |2 DE-101 | |
| 035 | |a (OCoLC)50457246 | ||
| 035 | |a (DE-599)BVBBV013098200 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng |a ger | |
| 049 | |a DE-20 |a DE-12 | ||
| 100 | 1 | |a Zhong, Jian |d 1961- |e Verfasser |0 (DE-588)122373464 |4 aut | |
| 245 | 1 | 0 | |a Apoptosis regulation by Raf-1 and Bcl-2 |c von Jian Zhong |
| 264 | 1 | |c 1999 | |
| 300 | |a 84 Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Würzburg, Univ., Diss., 2000. - Zsfassung in dt. Sprache | ||
| 650 | 7 | |a Hochschulschrift |2 gtt | |
| 650 | 0 | 7 | |a Apoptosis |0 (DE-588)4291096-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Regulation |0 (DE-588)4049075-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Mikroform |0 (DE-588)4039216-8 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Molekularbiologie |0 (DE-588)4039983-7 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Apoptosis |0 (DE-588)4291096-1 |D s |
| 689 | 0 | 1 | |a Regulation |0 (DE-588)4049075-0 |D s |
| 689 | 0 | 2 | |a Molekularbiologie |0 (DE-588)4039983-7 |D s |
| 689 | 0 | 3 | |a Mikroform |0 (DE-588)4039216-8 |D s |
| 689 | 0 | |8 1\p |5 DE-604 | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008921861&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008921861 | |
Datensatz im Suchindex
| _version_ | 1812461028155326464 |
|---|---|
| adam_text |
CONTENTS
SUMMARY ^
CHAPTER 1. INTRODUCTION 13
1-1. APOPTOSIS 13
1-2. THE BCL-2 FAMILY MEMBERS IN APOPTOSIS REGULATION 17
1-3. RAF-1 AND ITS ISOFORMS A-, AND B-RAF 19
1- 4. THE AIM OF THIS STUDY 21
CHAPTER 2. MATERIALS AND METHODS 23
2- 1. MATERIALS 23
2-1-1 CHEMICALS AND ENZYMES 23
2-1-2 ISOTOPE 25
2-1 -3. MOLECULAR WEIGHT MARKERS 25
2-1-4. BUFFERS 26
2-1-5. KITS 30
2-1-6. ANTIBODIES 31
2-1-7. PLASMIDS 32
2-1-8. VIRUSES 32
2-1-9. PCR-PRIMERS 32
5
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/959863656
2-1-10. OTHERS
33
2-1-11. ANIMALS 33
2-2 METHODS 34
2-2-1. ESTABLISHMENT OF PRIMARY CELL CULTURE 34
2-2-1 -1. ISOLATION OF EMBRYOS 34
2-2-1 -2. CELL LINES AND CULTURE CONDITIONS 34
2-2-1-3. ISOLATION OF GENOMIC DNA FROM EMBRYONIC TISSUES 35
2-2-1-4. GENOTYPING OF
BCL-2 DEFICIENT CELLS 35
2-2-1-5. GENOTYPING OF
RAF-L DEFICIENT CELLS 35
2-2-2. RETROVIRAL INFECTION 36
2-2-2-1. HARVEST AND TITRATION OF ONCOGEN CONTAINING VIRUSES 36
2-2-2-2. INFECTION 37
2-2-3. DNA TRANSFECTION 37
2-2-3-1. PLASMID CONSTRUCTS 37
2-2-3-2. AMPLIFICATION OF PLASMID DNA 37
2-2-3-3. PLASMID ISOLATION USING QIAGEN PROTOCOL 38
2-2-3-4 THE MEASUREMENT OF PLASMID CONCENTRATION 38
2-2-3-5. TRANSFECTION OF ADHERENT CELLS 39
2-2-4. DNA RELEASE ASSAY 39
2-2-5. ANALYSIS OF DNA FRAGMENTATION BY AGAROSE GEL ELECTROPHORESIS 40
2-2-6. THE MEASUREMENT OF PROTEIN CONCENTRATION 40
2-2-7. IMMUNOPRECIPITATION 41
6
2-2-8. WESTERN BLOT ANALYSIS 41
2-2-9 ANTISENSE EXPERIMENTS 41
2-2-10 RT-PCR OF
BAD 42
2-2-10-1. TOTAL RNA EXTRACTION USING THE Q1AGEN RNEASY MIDI KIT 42
2-2-10-2. CDNA SYNTHESIS USING 1ST STRAND CDNA SYNTHESIS KIT
FOR RT-PCR (AMV) 43
2- 2-10-3. RT-PCR 44
CHAPTER 3. RESULTS 45
3- 1. APOPTOSIS SUPPRESSION BY MITOCHONDRIA TARGETED ONCOGENIC
RAF-1 DOES NOT REQUIRE BCL-2 45
3-1-1. THE ESTABLISHMENT OF
BCL-2 DEFICIENT CELLS 45
3-1-2.
BCL-2 DEFICIENT FIBROBLASTS SHOW INCREASED SENSITIVITY TOWARDS
DIFFERENT CLASSES OF APOPTOTIC STIMULI 48
3-1 -3. OVEREXPRESSION OF BCL-2 INHIBITS DOXORUBICIN-INDUCED APOPTOSIS
49
3-1-4. BCL-2 BLOCKS CASPASE-3 ACTIVATION 50
3-1-5. GAG-V-RAF PROTEAS
BCL-2 DEFICIENT CELLS FROM APOPTOSIS
BY BLOCKING CASPASE-3 ACTIVATION 52
3-1-6. ACTIVATED RAF-1 REQUIRES MITOCHONDRIAL TRANSLOCATION
FOR APOPTOSIS SUPPRESSION 54
3-1-7. ABLATION OF RAF-1 EXPRESSION DOES NOT AFFEA DOXORUBICIN-INDUCED
CELL DEATH IN
BCL-2 DEFICIENT AND WILD TYPE CELLS 58
7
! 3-2. BCL-2 INHIBITS DOXOTUBICIN-INDUCED APOPTOSIS IN
RAF-1 DEFICIENT FIBROBLASTS 60
3-2-1. THE ESTABLISHMENT OF
RAF-1 DEFICIENT CELLS 60
3-2-2. THE
RAF-1 DEFICIENT CELLS SHOW INCREASED SENSITIVITY TOWARDS
DOXORUBICIN-INDUCED APOPTOSIS 61
3-2-3. THE OVEREXPRESSION OF BCL-2 INHIBITS DOXORUBICIN-INDUCED
APOPTOSIS
IN
RAF-1 AND WILD TYPE CELLS 64
CHAPTER 4. DISCUSSION 67
CHAPTER 5. REFERENCES 73
CHAPTER 6. ABBREVIATIONS 83
8 |
| any_adam_object | 1 |
| author | Zhong, Jian 1961- |
| author_GND | (DE-588)122373464 |
| author_facet | Zhong, Jian 1961- |
| author_role | aut |
| author_sort | Zhong, Jian 1961- |
| author_variant | j z jz |
| building | Verbundindex |
| bvnumber | BV013098200 |
| ctrlnum | (OCoLC)50457246 (DE-599)BVBBV013098200 |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>00000nam a2200000 c 4500</leader><controlfield tag="001">BV013098200</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20020228</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">000406s1999 ad|| m||| 00||| eng d</controlfield><datafield tag="016" ind1="7" ind2=" "><subfield code="a">959863656</subfield><subfield code="2">DE-101</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)50457246</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV013098200</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield><subfield code="a">ger</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-20</subfield><subfield code="a">DE-12</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Zhong, Jian</subfield><subfield code="d">1961-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)122373464</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Apoptosis regulation by Raf-1 and Bcl-2</subfield><subfield code="c">von Jian Zhong</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">84 Bl.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">Würzburg, Univ., Diss., 2000. - Zsfassung in dt. Sprache</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Hochschulschrift</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Apoptosis</subfield><subfield code="0">(DE-588)4291096-1</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Regulation</subfield><subfield code="0">(DE-588)4049075-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Mikroform</subfield><subfield code="0">(DE-588)4039216-8</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Molekularbiologie</subfield><subfield code="0">(DE-588)4039983-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Apoptosis</subfield><subfield code="0">(DE-588)4291096-1</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Regulation</subfield><subfield code="0">(DE-588)4049075-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Molekularbiologie</subfield><subfield code="0">(DE-588)4039983-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Mikroform</subfield><subfield code="0">(DE-588)4039216-8</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="8">1\p</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">DNB Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008921861&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="883" ind1="1" ind2=" "><subfield code="8">1\p</subfield><subfield code="a">cgwrk</subfield><subfield code="d">20201028</subfield><subfield code="q">DE-101</subfield><subfield code="u">https://d-nb.info/provenance/plan#cgwrk</subfield></datafield><datafield tag="943" ind1="1" ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-008921861</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV013098200 |
| illustrated | Illustrated |
| indexdate | 2024-10-09T18:12:10Z |
| institution | BVB |
| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008921861 |
| oclc_num | 50457246 |
| open_access_boolean | |
| owner | DE-20 DE-12 |
| owner_facet | DE-20 DE-12 |
| physical | 84 Bl. Ill., graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| record_format | marc |
| spelling | Zhong, Jian 1961- Verfasser (DE-588)122373464 aut Apoptosis regulation by Raf-1 and Bcl-2 von Jian Zhong 1999 84 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Würzburg, Univ., Diss., 2000. - Zsfassung in dt. Sprache Hochschulschrift gtt Apoptosis (DE-588)4291096-1 gnd rswk-swf Regulation (DE-588)4049075-0 gnd rswk-swf Mikroform (DE-588)4039216-8 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Apoptosis (DE-588)4291096-1 s Regulation (DE-588)4049075-0 s Molekularbiologie (DE-588)4039983-7 s Mikroform (DE-588)4039216-8 s 1\p DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008921861&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Zhong, Jian 1961- Apoptosis regulation by Raf-1 and Bcl-2 Hochschulschrift gtt Apoptosis (DE-588)4291096-1 gnd Regulation (DE-588)4049075-0 gnd Mikroform (DE-588)4039216-8 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4291096-1 (DE-588)4049075-0 (DE-588)4039216-8 (DE-588)4039983-7 (DE-588)4113937-9 |
| title | Apoptosis regulation by Raf-1 and Bcl-2 |
| title_auth | Apoptosis regulation by Raf-1 and Bcl-2 |
| title_exact_search | Apoptosis regulation by Raf-1 and Bcl-2 |
| title_full | Apoptosis regulation by Raf-1 and Bcl-2 von Jian Zhong |
| title_fullStr | Apoptosis regulation by Raf-1 and Bcl-2 von Jian Zhong |
| title_full_unstemmed | Apoptosis regulation by Raf-1 and Bcl-2 von Jian Zhong |
| title_short | Apoptosis regulation by Raf-1 and Bcl-2 |
| title_sort | apoptosis regulation by raf 1 and bcl 2 |
| topic | Hochschulschrift gtt Apoptosis (DE-588)4291096-1 gnd Regulation (DE-588)4049075-0 gnd Mikroform (DE-588)4039216-8 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Hochschulschrift Apoptosis Regulation Mikroform Molekularbiologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008921861&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT zhongjian apoptosisregulationbyraf1andbcl2 |