Fundamentals of enzymology: the cell and molecular biology of catalytic proteins
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Oxford [u.a.]
Oxford Univ. Press
1999
|
| Ausgabe: | 3. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVIII, 478 S., 4 [Bl.] Ill., graph. Darst. |
| ISBN: | 019850229X |
Internformat
MARC
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| 100 | 1 | |a Price, Nicholas C. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Fundamentals of enzymology |b the cell and molecular biology of catalytic proteins |c Nicholas C. Price and Lewis Stevens |
| 250 | |a 3. ed. | ||
| 264 | 1 | |a Oxford [u.a.] |b Oxford Univ. Press |c 1999 | |
| 300 | |a XVIII, 478 S., 4 [Bl.] |b Ill., graph. Darst. | ||
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| 650 | 4 | |a Enzymes | |
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Datensatz im Suchindex
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|---|---|
| adam_text | Contents
The plates fall between pages 174 and 175.
A note on units xv
List of abbreviations xvii
chapter 1 Introduction 1
11 Aims of this book 1
12 Historical aspects 1
1.3 Remarkable properties of enzymes as catalysts 4
1 4 Cofactors 7
15 Nomenclature and classification of enzymes 8
1.6 The contents of this book 13
References 14
chapter 2 The purification of enzymes 1 b
2 1 Introduction 15
2 2 Why isolate enzymes? 15
2 3 Objectives and strategy in enzyme purification 16
2 4 Choice of source 17
2 5 Methods of homogenization 20
2 6 Methods of separation 21
2 7 How to judge the success of a purification procedure 34
2 8 Examples of purification procedures 36
2 9 Conclusions from the examples of enzyme purification 43
References 44
chapter 3 The structure of enzymes 47
3.1 Introduction 47
3.2 The determination of Mr 48
3.3 The determination of amino acid composition and primary structure 54
CONTENTS
3.4 The determination of secondary and tertiary structure 75
3.5 The determination of quaternary structure 100
3.6 The unfolding and folding of enzymes 106
3.7 Concluding remarks 110
References 111
Appendix 3.1 116
References for Appendix 3.1 117
Chapter4 An introduction to enzyme kinetics us
4.1 Outline of the chapter 118
4.2 How do we obtain kinetic data? 119
4.3 How do we analyse kinetic data? 121
4.4 Pre steady state kinetics 144
4 5 Concluding remarks 148
References 148
Appendix 4.1 150
Appendix 4.2 151
Appendix 4.3 152
References for Appendices 153
chapter 5 The mechanism of enzyme action 154
5.1 Introduction 154
5 2 Definition of the mechanism of an enzyme catalysed reaction 154
5.3 Background to catalysis 155
5 4 Experimental approaches to the determination of enzyme
mechanisms 162
5 5 Examples of enzyme mechanisms 177
5 6 Concluding comments on enzyme mechanisms 212
References 212
chapter 6 The control of enzyme activity 217
6.1 Introduction 217
6 2 Control of the activities of single enzymes 218
6 3 Control of metabolic pathways 237
6 4 Examples of control of metabolic pathways 249
6 5 Concluding remarks 266
References 266
Appendix 6.1 270
chapter 7 Enzymes in organized systems 273
7 1 Introduction 273
7 2 Organized enzyme systems 273
CONTENTS Xlii
7 3 RNA polymerase from E. coli 21A
1A The occurrence and isolation of multienzyme proteins 281
7 5 Phylogenetic distribution of multienzyme proteins 282
7.6 Properties of multienzyme proteins 283
7.7 Pyruvate dehydrogenase multienzyme complex and related systems 285
7 8 Glycine decarboxylase multienzyme complex 294
7 9 The tryptophan synthase multienzyme complex from E. coli 294
7.10 Carbamoyl phosphate synthase and the associated enzymes of the
pyrimidine and arginine biosynthetic pathways in E. coli, fungi, and
mammalian cells 300
7.11 Multienzyme polypeptides: fatty acid synthase and the arom complex
(AROM enzyme) 304
7 12 Enzymes involved in DNA synthesis 315
7.13 The glycogen particle 315
7.14 Conclusions 317
References 317
chapter 8 Enzymes in the cell 321
8.1 Introduction 321
8.2 Intracellular compartmentation 322
8.3 Compartmentation of metabolic pathways 333
8.4 Vectorial organization of enzymes associated with membranes 347
8.5 The concentrations of enzymes and substrates in vivo 355
8.6 Conclusions 366
References 367
Chapter 9 Enzyme turnover 370
9 1 Introduction 370
9 2 Kinetics of enzyme turnover 371
9 3 Methods for measurement of rates of enzyme turnover 373
9 4 Results from measurements of rates of enzyme turnover 375
9 5 Possible correlations between the rates of turnover and the structure and
function of enzymes 376
9 6 The mechanisms of protein degradation 377
9 7 The significance of enzyme turnover 389
9 8 Other processes in which intracellular proteolysis is important 394
9.9 Conclusions 396
References 396
Appendix 9.1 399
chapter 1 o Clinical aspects of enzymology 400
10.1 Introduction 400
10 2 Determination of enzyme activities for clinical diagnosis 401
CONTENTS
10.3 Clinical enzymology of liver disease 404
10.4 Clinical enzymology of heart disease 406
10.5 Other enzyme activities that become elevated in serum in disease 408
10.6 The detection and significance of enzyme deficiencies 411
10.7 Enzyme inhibitors and drug design 415
10.8 The use of enzymes to determine the concentrations of metabolites of
clinical importance 416
10.9 Enzyme therapy 424
10.10 Conclusions 428
References 428
chapter 11 Enzyme technology 430
11.1 Introduction 430
11.2 Use of microorganisms in brewing and cheesemaking 430
11.3 Use of microorganisms in the production of organic chemicals 433
11.4 Use of isolated enzymes in industrial processes 433
11.5 Immobilized enzymes 440
References 449
Appendix: Enzymes referred to in Chapters 1 11 450
Index 471
|
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| indexdate | 2024-07-09T18:36:59Z |
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| isbn | 019850229X |
| language | English |
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| physical | XVIII, 478 S., 4 [Bl.] Ill., graph. Darst. |
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| spelling | Price, Nicholas C. Verfasser aut Fundamentals of enzymology the cell and molecular biology of catalytic proteins Nicholas C. Price and Lewis Stevens 3. ed. Oxford [u.a.] Oxford Univ. Press 1999 XVIII, 478 S., 4 [Bl.] Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Celbiologie gtt Enzimas larpcal Enzimologia larpcal Enzymen gtt Enzymes Moleculaire biologie gtt Enzym (DE-588)4014988-2 gnd rswk-swf Enzymologie (DE-588)4152482-2 gnd rswk-swf Enzym (DE-588)4014988-2 s DE-604 Enzymologie (DE-588)4152482-2 s Stevens, Lewis Verfasser aut HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008836089&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Price, Nicholas C. Stevens, Lewis Fundamentals of enzymology the cell and molecular biology of catalytic proteins Celbiologie gtt Enzimas larpcal Enzimologia larpcal Enzymen gtt Enzymes Moleculaire biologie gtt Enzym (DE-588)4014988-2 gnd Enzymologie (DE-588)4152482-2 gnd |
| subject_GND | (DE-588)4014988-2 (DE-588)4152482-2 |
| title | Fundamentals of enzymology the cell and molecular biology of catalytic proteins |
| title_auth | Fundamentals of enzymology the cell and molecular biology of catalytic proteins |
| title_exact_search | Fundamentals of enzymology the cell and molecular biology of catalytic proteins |
| title_full | Fundamentals of enzymology the cell and molecular biology of catalytic proteins Nicholas C. Price and Lewis Stevens |
| title_fullStr | Fundamentals of enzymology the cell and molecular biology of catalytic proteins Nicholas C. Price and Lewis Stevens |
| title_full_unstemmed | Fundamentals of enzymology the cell and molecular biology of catalytic proteins Nicholas C. Price and Lewis Stevens |
| title_short | Fundamentals of enzymology |
| title_sort | fundamentals of enzymology the cell and molecular biology of catalytic proteins |
| title_sub | the cell and molecular biology of catalytic proteins |
| topic | Celbiologie gtt Enzimas larpcal Enzimologia larpcal Enzymen gtt Enzymes Moleculaire biologie gtt Enzym (DE-588)4014988-2 gnd Enzymologie (DE-588)4152482-2 gnd |
| topic_facet | Celbiologie Enzimas Enzimologia Enzymen Enzymes Moleculaire biologie Enzym Enzymologie |
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