Adrenergic receptor protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2000
|
| Schriftenreihe: | Methods in molecular biology
126 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIV, 577 S. Ill., graph. Darst. |
| ISBN: | 0896036022 |
Internformat
MARC
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| 245 | 1 | 0 | |a Adrenergic receptor protocols |c ed. by Curtis A. Machida |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2000 | |
| 300 | |a XIV, 577 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 126 | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a Adrenaline |x Receptors |v Laboratory manuals | |
| 650 | 4 | |a Gene Expression Regulation | |
| 650 | 4 | |a Receptors, Adrenergic |x analysis | |
| 650 | 4 | |a Receptors, Adrenergic |x genetics | |
| 650 | 4 | |a Sequence Analysis, RNA |x methods | |
| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Adrenerger Rezeptor |0 (DE-588)4000526-4 |2 gnd |9 rswk-swf |
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| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Machida, Curtis A |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 126 |w (DE-604)BV035362695 |9 126 | |
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Datensatz im Suchindex
| _version_ | 1804127628636979200 |
|---|---|
| adam_text | Contents
Preface v
List of Contributors xi
Part I. Genetic Analysis 7
1 Construction of Libraries for Isolation of Adrenergic Receptor Genes
Margaret A. Scofield, Jean D. Deupree, and David B. Bylund 3
2 Isolation of Adrenergic Receptor Genes
Margaret A. Scofield, Jean D. Deupree, and David B. Bylund 37
3 Analyses of Adrenergic Receptor Sequences
Jean D. Deupree, Margaret A. Scofield, and David B. Bylund 53
4 Polymerase Chain Reaction Screening of Genomic Libraries
for Adrenergic Receptor Genes
DianneM. Perez and Michael J. Zuscik 73
5 Solution Phase Library Screening for Identification
of Rare Adrenergic Receptor Clones
DianneM. Perez and Michael J. Zuscik 91
6 Genetic Polymorphisms of Adrenergic Receptors
Ian P. Hall, Amanda P. Wheatley, and Jane C. Dewar 7 77
Part II. RNA Analysis 727
7 Northern Blot Analyses Detecting Adrenergic Receptor mRNAs
Judith C. W. Mak 129
8 Ribonuclease Protection Assay for the Detection of p, Adrenergic
Receptor RNA
Yong Feng Yang and Curtis A. Machida 739
9 Determination of Adrenergic Receptor mRNAs by Quantitative
Reverse Transcriptase Polymerase Chain Reactions
Stefan Engelhardt and Martin J. Lohse 755
10 Nuclear Run On Assays for Measurement of Adrenergic Receptor
Transcription Rate
Zhuo Wei Hu and Brian B. Hoffman 769
vii
vijj Contents
11 Primer Extension Methods for Determination of (3, Adrenergic
Receptor mRNA Start Sites
Yi Tang Tseng and James F. Padbury 181
Part III. Expression Analysis 187
12 Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors
Stephane Schaak, Jean Christophe Devedjian,
and Herve Paris 189
13 Expression of p Adrenergic Receptors in Recombinant
Baculovirus lnfected Insect Cells
A. Donny Strosberg and Jean Luc Guillaume 207
14 Expression of p Adrenergic Receptors in E. coli
Ralf Jockers and A. Donny Strosberg 215
15 Use and Pharmacological Analysis of Established
and Transfected Cell Lines Expressing Adrenergic Receptors
Hongying Zhong and Kenneth P. Minneman 221
16 Transient Transfection and Adrenergic Receptor Promoter Analysis
Yi Tang Tseng and James F. Padbury 235
17 Antisense RNA/DNA Based Techniques to Probe Adrenergic
Receptor Function
Hsien Yu Wang, Fubao Lin, and Craig C. Malbon 241
18 Targeted Disruption of Adrenergic Receptor Genes
Daniel K. Rohrer 259
Part IV. Receptor and Second Messenger Analysis 279
19 Development of Antibodies to Adrenergic Receptors
Suleiman W. Bahouth 281
20 Western Blot Detection of Adrenergic Receptors
Suleiman W. Bahouth 301
21 UV Crosslinking of Adrenergic Receptors and Ligands:
Detection by SDS PAGE
Michael K. Sievert, John F. Resek, Zhongren Wu,
Yajing Rong, and Arnold E. Ruoho 315
22 Detection of p Adrenergic Receptors by Radioligand Binding
Cheryl D. Dunigan, Patricia K. Curran, and Peter H. Fishman ... 329
23 Assessing Adrenergic Receptor Conformation Using Chemically
Reactive Fluorescent Probes
Anne Dam Jensen and Ulrik Gether 345
24 Biochemical Methods for Detection and Measurement
of Cyclic AMP and Adenylyl Cyclase Activity
Steven R. Post, Rennolds S. Ostrom, and Paul A. Insel 363
Contents ix
25 Assay of Arachidonic Acid Release Coupled
to a, and a2 Adrenergic Receptors
Lincoln Edwards and Paul Ernsberger 375
26 Patch Clamp Recording Methods for Examining Adrenergic
Regulation of Potassium Currents in Ocular Epithelial Cells
Jennifers. Ryan, Chanjuan Shi, andMelanie E. M. Kelly 391
Part V. Transactivator Analysis 409
27 Southwestern Blots for Detection of a DNA Binding Protein
Recognizing the a1B Adrenergic Receptor Gene Promoter
Bin Gao and George Kunos 411
28 DNase I Footprinting Analysis of Transcription Factors Recognizing
Adrenergic Receptor Gene Promoter Sequences
Bin Gao and George Kunos 419
29 Electrophoretic Mobility Shift Assay for Detection of DNA Binding
Proteins Recognizing p Adrenergic Receptor Gene Sequences
Philbert Kirigiti and Curtis A. Machida 431
30 Determination of mRNA Stability and Characterization of Proteins
Interacting with Adrenergic Receptor mRNAs
Burns C. Blaxall and J. David Port 453
Part VI. Microscopy Analysis 467
31 Use of Immunohistochemistry and Confocal Microscopy
in the Detection of Adrenergic Receptors
Ruth L. Stornetta 469
32 Distribution of a2A and a2C Adrenergic Receptor Immunoreactivity
in the Central Nervous System
Diane L. Rosin 475
33 Quantitative Light Microscopic Autoradiography of x2 Adrenergic
Radioligand Binding Sites
Ursula H. Winzer Serhan and Frances M. Leslie 50 7
34 In Situ Hybridization of Adrenergic Receptor mRNA in Brain
Mark Stafford Smith and Debra A. Schwinn 519
35 Use of Electron Microscopy in the Detection of Adrenergic Receptors
Chiye A oki, Sarina Rodrigues, and Hitoshi Kurose 535
Index 565
|
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| genre_facet | Aufsatzsammlung |
| id | DE-604.BV012939144 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:36:22Z |
| institution | BVB |
| isbn | 0896036022 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008809103 |
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| physical | XIV, 577 S. Ill., graph. Darst. |
| publishDate | 2000 |
| publishDateSearch | 2000 |
| publishDateSort | 2000 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Adrenergic receptor protocols ed. by Curtis A. Machida Totowa, NJ Humana Press 2000 XIV, 577 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 126 Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Adrenaline Receptors Laboratory manuals Gene Expression Regulation Receptors, Adrenergic analysis Receptors, Adrenergic genetics Sequence Analysis, RNA methods Methode (DE-588)4038971-6 gnd rswk-swf Adrenerger Rezeptor (DE-588)4000526-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Adrenerger Rezeptor (DE-588)4000526-4 s Methode (DE-588)4038971-6 s DE-604 Machida, Curtis A Sonstige oth Methods in molecular biology 126 (DE-604)BV035362695 126 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008809103&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Adrenergic receptor protocols Methods in molecular biology Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Adrenaline Receptors Laboratory manuals Gene Expression Regulation Receptors, Adrenergic analysis Receptors, Adrenergic genetics Sequence Analysis, RNA methods Methode (DE-588)4038971-6 gnd Adrenerger Rezeptor (DE-588)4000526-4 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4000526-4 (DE-588)4143413-4 |
| title | Adrenergic receptor protocols |
| title_auth | Adrenergic receptor protocols |
| title_exact_search | Adrenergic receptor protocols |
| title_full | Adrenergic receptor protocols ed. by Curtis A. Machida |
| title_fullStr | Adrenergic receptor protocols ed. by Curtis A. Machida |
| title_full_unstemmed | Adrenergic receptor protocols ed. by Curtis A. Machida |
| title_short | Adrenergic receptor protocols |
| title_sort | adrenergic receptor protocols |
| topic | Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Adrenaline Receptors Laboratory manuals Gene Expression Regulation Receptors, Adrenergic analysis Receptors, Adrenergic genetics Sequence Analysis, RNA methods Methode (DE-588)4038971-6 gnd Adrenerger Rezeptor (DE-588)4000526-4 gnd |
| topic_facet | Wetenschappelijke technieken Wissenschaftliches Arbeiten Adrenaline Receptors Laboratory manuals Gene Expression Regulation Receptors, Adrenergic analysis Receptors, Adrenergic genetics Sequence Analysis, RNA methods Methode Adrenerger Rezeptor Aufsatzsammlung |
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