RNA protein interaction protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1999
|
| Schriftenreihe: | Methods in molecular biology
118 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XIX, 481 S. Ill., graph. Darst. |
| ISBN: | 0896035689 |
Internformat
MARC
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| 245 | 1 | 0 | |a RNA protein interaction protocols |c ed. by Susan R. Haynes |
| 246 | 1 | 3 | |a RNA-protein interaction protocols |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1999 | |
| 300 | |a XIX, 481 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 490 | 1 | |a Methods in molecular biology |v 118 | |
| 650 | 2 | |a Protéines - analyse | |
| 650 | 2 | |a Ribonucléoprotéine - analyse | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a RNA |x chemistry |v Laboratory Manuals | |
| 650 | 4 | |a RNA-Binding Proteins |x analysis |v Laboratory Manuals | |
| 650 | 4 | |a RNA-protein interactions |x Laboratory manuals | |
| 650 | 4 | |a Ribonucleoproteins |x analysis |v Laboratory Manuals | |
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Datensatz im Suchindex
| _version_ | 1804127564879364097 |
|---|---|
| adam_text | Contents
Preface v
Contributors xv
1 Labeling and Purification of RNA Synthesized
by In Vitro Transcription 1
Paul A. Clarke
2 Joining RNA Molecules with T4 DNA Ligase 11
Melissa J. Moore
3 RNA Protein Crosslinking with Photoreactive Nucleotide Analogs ... 21
Michelle M. Hanna, Lori Bentsen, Michael Lucldo,
and Archana Sapre
4 The Methylene Blue Mediated Photocrosslinking Method for
Detection of Proteins that Interact with Double Stranded RNA 35
Zhi Ren Liu and Christopher W. J. Smith
5 Probing RNA Protein Interactions by Psoralen Photocrosslinking .... 49
Zhuying Wang and Tariq M. Rana
6 Identification and Sequence Analysis of RNA Protein Contact Sites
by N Terminal Sequencing and MALDI MS 63
Bernd Thiede, Henning Urlaub, Helga Neubauer,
and Brigitte Wittmann Liebold
7 RNA Footprinting and Modification Interference Analysis 73
Paul A. Clarke
8 Oligonucleotide Targeted RNase H Protection Analysis
of RNA Protein Complexes 93
Arthur Gunzl and Albrecht Bindereif
9 Nitrocellulose Filter Binding for Determination
of Dissociation Constants 105
Kathleen B. Hall and James K. Kranz
10 Measuring Equilibrium and Kinetic Constants
Using Gel Retardation Assays 115
David R. Setzer
11 PACE Analysis of RNA Peptide Interactions 129
Christopher D. Cilley and James R. Williamson
xi
xii Contents
12 Detection of Nucleic Acid Interactions Using Surface
Plasmon Resonance 143
Robert J. Crouch, Makoto Wakasa, and Mitsuru Haruki
13 An Escherichia co// Based Genetic Strategy for Characterizing
RNA Binding Proteins 161
Chaitanya Jain
14 Screening RNA Binding Libraries Using a Bacterial
Transcription Antitermination Assay 177
Kazuo Harada and Alan D. Frankel
15 In Vitro Genetic Analysis of RNA Binding Proteins
Using Phage Display 189
Ite A. Laird Offringa
16 In Vitro Selection of Aptamers from RNA Libraries 217
Daniel J. Kenan and Jack D. Keene
17 Identification of Specific Protein RNA Target Sites
Using Libraries of Natural Sequences 233
Lucy G. Andrews and Jack D. Keene
18 Northwestern Screening of Expression Libraries 245
Paramjeet S. Bagga and Jeffrey Wilusz
19 Screening Expression Libraries with Solution Based Assay 257
Philippa J. Webster and Paul M. Macdonald
20 An lmmunoprecipitation RNA:rPCR Method
for the In Vivo Isolation of Ribonucleoprotein Complexes 265
Edward Chu, John C. Schmitz, Jingfang Ju, and Sitki M. Copur
21 Purification and Depletion of RNP Particles by Antisense Affinity ;
Chromatography 275
Benjamin J. Blencowe and Angus I. Lamond
22 Purification of U Small Nuclear Ribonucleoprotein Particles 289
Berthold Kastner and Reinhard Liihrmann
23 Preparation of Heterogeneous Nuclear Ribonucleoprotein
Complexes 299
Maurice S. Swanson and Gideon Dreyfuss
24 Preparation of Heia Cell Nuclear and Cytosolic S100 Extracts ;
for In Vitro Splicing 309
Akila Mayeda and Adrian R. Krainer
25 Mammalian In Vitro Splicing Assays 315 ¦
Akila Mayeda and Adrian R. Krainer I
Contents xiii
26 Yeast Pre mRNA Splicing Extracts 323
Stephanie W. Ruby
27 Prespliceosome and Spliceosome Isolation and Analysis 351
Laura A. Lindsey and Mariano A. Garcia Blanco
28 A Yeast Spliceosome Assay 365
Stephanie W. Ruby
29 Defining Pre mRNA cis Elements that Regulate
Cell Specific Splicing 391
Thomas A. Cooper
30 In Vivo SELEX in Vertebrate Cells 405
Thomas A. Cooper
31 Purification of SR Protein Splicing Factors 419
Alan M. Zahler
32 Processing mRNA 3 Ends In Vitro 433
Michael J. Imperiale
33 Analysis of Poly(A) Tail Lengths by PCR: The PAT Assay 441
Fernando J. Saltes and Sidney Strickland
34 In Vitro Translation Extracts from Tissue Culture Cells 449
Kazuko Shiroki andAkio Nomoto
35 Messenger RNA Turnover in Cell Free Extracts
from Higher Eukaryotes 459
Jeff Ross
Index 477
|
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| id | DE-604.BV012878781 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:35:22Z |
| institution | BVB |
| isbn | 0896035689 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008766542 |
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| physical | XIX, 481 S. Ill., graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | RNA protein interaction protocols ed. by Susan R. Haynes RNA-protein interaction protocols Totowa, NJ Humana Press 1999 XIX, 481 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 118 Protéines - analyse Ribonucléoprotéine - analyse Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten RNA chemistry Laboratory Manuals RNA-Binding Proteins analysis Laboratory Manuals RNA-protein interactions Laboratory manuals Ribonucleoproteins analysis Laboratory Manuals RNS-Bindungsproteine (DE-588)4178253-7 gnd rswk-swf Wechselwirkung (DE-588)4064937-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS-Bindungsproteine (DE-588)4178253-7 s Wechselwirkung (DE-588)4064937-4 s DE-604 Haynes, Susan R. Sonstige oth Methods in molecular biology 118 (DE-604)BV035362695 118 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008766542&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | RNA protein interaction protocols Methods in molecular biology Protéines - analyse Ribonucléoprotéine - analyse Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten RNA chemistry Laboratory Manuals RNA-Binding Proteins analysis Laboratory Manuals RNA-protein interactions Laboratory manuals Ribonucleoproteins analysis Laboratory Manuals RNS-Bindungsproteine (DE-588)4178253-7 gnd Wechselwirkung (DE-588)4064937-4 gnd |
| subject_GND | (DE-588)4178253-7 (DE-588)4064937-4 (DE-588)4143413-4 |
| title | RNA protein interaction protocols |
| title_alt | RNA-protein interaction protocols |
| title_auth | RNA protein interaction protocols |
| title_exact_search | RNA protein interaction protocols |
| title_full | RNA protein interaction protocols ed. by Susan R. Haynes |
| title_fullStr | RNA protein interaction protocols ed. by Susan R. Haynes |
| title_full_unstemmed | RNA protein interaction protocols ed. by Susan R. Haynes |
| title_short | RNA protein interaction protocols |
| title_sort | rna protein interaction protocols |
| topic | Protéines - analyse Ribonucléoprotéine - analyse Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten RNA chemistry Laboratory Manuals RNA-Binding Proteins analysis Laboratory Manuals RNA-protein interactions Laboratory manuals Ribonucleoproteins analysis Laboratory Manuals RNS-Bindungsproteine (DE-588)4178253-7 gnd Wechselwirkung (DE-588)4064937-4 gnd |
| topic_facet | Protéines - analyse Ribonucléoprotéine - analyse Wetenschappelijke technieken Wissenschaftliches Arbeiten RNA chemistry Laboratory Manuals RNA-Binding Proteins analysis Laboratory Manuals RNA-protein interactions Laboratory manuals Ribonucleoproteins analysis Laboratory Manuals RNS-Bindungsproteine Wechselwirkung Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008766542&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT haynessusanr rnaproteininteractionprotocols |