Plant conservation biotechnology:
Gespeichert in:
Format: | Buch |
---|---|
Sprache: | English |
Veröffentlicht: |
London [u.a.]
Taylor & Francis
1999
|
Schlagworte: | |
Online-Zugang: | Inhaltsverzeichnis |
Beschreibung: | XXV, 309 S. Ill., graph. Darst. |
ISBN: | 0748407464 |
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245 | 1 | 0 | |a Plant conservation biotechnology |c ed. by Erica E. Benson |
264 | 1 | |a London [u.a.] |b Taylor & Francis |c 1999 | |
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650 | 7 | |a Plantes - Conservation |2 ram | |
650 | 7 | |a Plantes - Ressources génétiques |2 ram | |
650 | 4 | |a Biotechnology |x methods | |
650 | 4 | |a Cryopreservation | |
650 | 4 | |a Germplasm resources, Plant | |
650 | 4 | |a Plant biotechnology | |
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Datensatz im Suchindex
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---|---|
adam_text | Plant Conservation
Biotechnology
Edited by
ERICA E BENSON
University of Abertay, Dundee UK
Contents
List of Figures page xiii
Foreword xv
Preface xvii
Acknowledgements xix
Contributing Authors xxi
PART ONE Principles of Plant Conservation Biotechnology:
Methods, Techniques and Procedures 1
An Introduction to Plant Conservation Biotechnology 3
Erica E Benson
1 1 Integrating biotechnology into conservation programmes 3
12A general overview: how does biotechnology assist plant
conservation? 4
1 3 Conservation biotechnology and the sustainable utilization of
plant genetic resources 7
1 4 Conclusions and future prospects 8
References 9
Molecular Approaches to Assessing Plant Diversity 11
Stephen A Harris
2 1 Introduction 11
2 2 Molecular marker systems 12
2 3 Molecular markers in germplasm characterization 16
2 4 Molecular markers in systematics and population genetics 17
2 5 Prospects for molecular markers in biodiversity characterization 18
References 19
Biotechnology in Plant Germplasm Acquisition 25
Kim E Hummer
3 1 Introduction 25
3 2 Plant genetic resource conservation 25
vi Contents
321 In situ conservation 26
322 Ex situ conservation 26
323 Static and dynamic conservation 27
3 3 Acquisition procedures 27
331 Plant exploration 27
332 Plant exchange 28
3 4 Acquisition planning 29
341 Intellectual property rights 30
342 Quarantine regulations 31
3 5 Methods of acquisition 32
351 Seeds 32
352 Vegetative propagation 32
3 6 Documentation 34
3 7 Identity confirmation 34
3 8 Summary 36
References 36
4 Tissue Culture Techniques in In Vitro Plant Conservation 41
Paul T Lynch
4 1 Introduction 41
4 2 In vitro propagation 41
421 Germplasm acquisition 43
422 Selection of tissue for in vitro culture 43
423 Microbial contamination and disease indexing 44
424 Tissue culture media 45
425 Problems of culture establishment 48
426 Propagule multiplication (morphogenesis) 49
427 Plantlet development 50
4 3 Acclimatization of in vitro germplasm to in vivo conditions 50
4 4 In vitro culture recalcitrance 52
4 5 Embryo rescue 52
4 6 Use of plant tissue culture for germplasm storage 53
461 Slow (minimal) growth 53
462 Recovery of germplasm after storage 53
4 7 Facilities for plant tissue culture 54
4 8 Conclusions 54
References 55
5 Phytosanitary Aspects of Plant Germplasm Conservation 63
Robert R Martin and Joseph D Postman
5 1 Introduction 63
5 2 Safe movement of germplasm 63
521 Quarantine 64
522 International cooperation 64
5 3 International guidelines 67
5 4 Virus detection 68
541 Requirements for detection and diagnosis 69
542 Serological detection 70
543 Nucleic acid based assays 71
Contents vii
544 Detection based on more traditional methods 72
545 The significance of a test result 73
5 5 Production of pathogen-free plants 73
551 Heat therapy 74
552 Meristem tip culture 75
553 Chemotherapy 76
5 6 Conclusions 78
References 78
6 Cryopreservation 83
Erica E Benson
6 1 Introduction 83
6 2 Principles of cryopreservation and germplasm preparation 83
621 Preparing germplasm for cryopreservation 84
622 Pre-treatments 85
6 3 Cryprotection 85
631 Traditional cryoprotection and controlled rate cooling 86
632 Vitrification 88
6 4 Freezing and long-term cryogenic storage 89
641 Controlled rate freezing 89
642 Rapid freezing and long-term storage 90
6 5 Post-cryopreservation recovery 90
6 6 Cryopreservation protocols: techniques and practical considerations 91
6 7 Conclusions 93
References 93
7 Stability Assessments of Conserved Plant Germplasm 97
Keith Harding
7 1 Introduction 97
7 2 Natural variation in populations 97
7 3 Techniques to assess genetic stability 98
7 4 Morphological variation 98
7 5 Cytological analysis 99
7 6 Biochemical analysis 99
7 7 Molecular analysis 99
771 Genome structure 100
772 Techniques 100
7 8 Conclusion 103
References 103
PART TWO Applications of Biotechnology in Plant Diversity
Conservation 109
8 Conservation Strategies for Algae 111
John G Day
8 1 Introduction 111
8 2 Alternative strategies employed to conserve algae 112
8 3 Roles of genetic resource centres and culture collections 113
8 4 Methodological strategies employed to conserve algae 113
viii Contents
841 Maintenance by serial subculture 113
842 Maintenance by storage in liquid medium 115
843 Drying techniques 115
844 Cryopreservation Techniques 116
8 5 Concluding comments 120
Acknowledgements 121
References 121
9 Cryo-conservation of Industrially Important Plant Cell Cultures 125
Heinz Martin Schumacher
9 1 Introduction: the biotechnological use of dedifferentiated plant
cell cultures 125
9 2 Stability of product formation after cryopreservation 126
921 Anthocyanins 126
922 Ginsenosides 127
923 Diosgenine 128
924 Rosmarinic acid 128
925 Biotin 129
926 Indole alkaloids 129
927 Berberine 131
9 3 Stability of a biosynthetic capacity: cardiac glycosides 131
9 4 Transformed root cultures for secondary metabolite production 132
9 5 Conclusions 133
References 136
10 In Vitro Conservation of Temperate Tree Fruit and Nut Crops 139
Barbara M Reed
10 1 Introduction 139
10 2 Literature review of progress 140
10 2 1 Medium-term storage at above freezing temperatures 140
10 2 2 Long-term storage in liquid nitrogen 142
10 3 Germplasm storage 145
10 3 1 In vitro stored collections 145
10 3 2 Cryopreserved collections 145
10 3 3 Discussion: the role of storage technologies 146
10 4 Impact on the storage and distribution of germplasm 148
10 4 1 In vitro storage 148
10 4 2 Cryopreservation 148
10 5 Conclusions 149
References 149
11 Conservation of Small Fruit Germplasm 155
Rex M Brennan and Stephen Millam
11 1 Introduction 155
11 1 1 Small fruit germplasm collections 155
11 2 Small fruit breeding 156
11 3 Problems related to field-based collections 156
11 4 World-wide small fruit germplasm collections 157
11 5 In vitro conservation and cryopreservation 158
Contents ix
11 5 1 Tissue culture 158
11 5 2 Cold storage of tissue cultures 159
11 5 3 Cryopreservation 159
11 6 The use of pollen storage for small fruit germplasm conservation 160
11 7 Conservation of transgenic plant small fruit plant germplasm 161
11 8 Genetic stability aspects 161
11 9 DNA banking 162
11 10 Conclusions 162
Acknowledgements 162
References 162
12 Biotechnological Advances in the Conservation of Root and
Tuber Crops 165
Ali M Colmirzaie, Ana Panta and Judith Toledo
12 1 Introduction 165
12 2 Establishment of aseptic cultures 166
12 2 1 Infection produced by viroids 167
12 2 2 Infection produced by viruses 167
12 2 3 Infection produced by systemic bacteria 167
12 3 In vitro maintenance 168
12 3 1 Short-term storage 168
12 3 2 Long-term storage 168
12 3 3 In vitro tuberization for long-term conservation 170
12 4 Other operational considerations for germplasm maintenance 170
12 5 Advances in germplasm utilization 171
12 6 Cryopreservation 172
12 6 1 Potato cryopreservation 172
12 6 2 Sweet potato cryopreservation 175
12 7 Genetic stability 175
12 8 Conclusions 175
References 176
13 Biotechnology in Germplasm Management of Cassava and Yams 179
SYC Ng, S H Mantel! and N Q Ng
13 1 Introduction 179
13 1 1 Origin, distribution and cultivation 179
13 1 2 Genetic diversity 180
13 2 Integrated strategy for germplasm conservation 181
13 2 1 In situ conservation 181
13 2 2 Ex situ conservation 182
13 3 Tissue culture methods for germplasm conservation and exchange 183
13 3 1 Meristem/shoot-tip culture 183
13 3 2 Axillary bud and nodal cultures 184
13 3 3 Embryo culture 184
13 3 4 Microtuberization 185
13 3 5 Adventitive regeneration 185
13 3 6 Reduced growth storage 186
13 3 7 Cryopreservation 187
x Contents
13 4 Biochemical and molecular fingerprinting for auditing of
germplasm collection 187
13 4 1 Range of methods available 187
13 4 2 Isozyme fingerprinting 188
13 4 3 Use of RAPD-PCR, microsatellites and other molecular
markers 190
13 5 Applications to germplasm conservation, exchange, and
improvement 192
13 5 1 Germplasm exchange 192
13 5 2 Germplasm conservation 197
13 5 3 Conservation biotechnology and germplasm improvement 199
13 6 Conclusions and future prospects 201
References 202
14 Conservation Biotechnology of Endemic and other Economically
Important Plant Species of India 211
Binay B Mandal
14 1 Introduction 211
14 2 In vitro techniques in conservation 212
14 3 Propagation 212
14 3 1 Somatic embryogenesis 214
14 3 2 Production of storage organs in vitro 214
14 4 Medium-term conservation 214
14 5 Conservation at normal culture room temperature (25°C) 217
14 5 1 Conservation under normal growing conditions 217
14 5 2 Conservation with media manipulation 217
14 5 3 Conservation using induced storage organs 217
14 6 Long-term conservation using cryopreservation 218
14 6 1 Cryopreservation of recalcitrant seed species 219
14 6 2 Cryopreservation of clonally propagated species 219
14 7 Characterization, classification and monitoring 220
14 7 1 Characterization and classification of germplasm 220
14 7 2 Monitoring genetic stability of conserved germplasm 221
14 8 In vitro conservation activities at other research stations in India 222
14 9 Conclusions 223
References 223
15 The Application of Biotechnology for the Conservation of
Endangered Plants 227
Valerie C Pence
15 1 Introduction 227
15 2 Special aspects of endangered species work 227
15 3 In vitro propagation technologies and endangered plant species 228
15 3 1 In vitro propagation using seed 228
15 3 2 Propagation without seeds 235
15 4 Genetic diversity and genetic stability 236
15 5 Uses of in vitro propagated plants of endangered species 236
15 6 Application of preservation technologies 237
15 7 Application of in vitro collection biotechnology 239
Contents xi
15 8 Application of genetic analysis and molecular techniques to
endangered plant species conservation 240
15 9 Summary 241
References 241
16 Conservation of the Rare and Endangered Plants Endemic to Spain 251
M E Gonzalez-Benito, C Martin, J M Iriondo and C Perez
16 1 Introduction 251
16 2 The application of micropropagation and in vitro conservation to
endangered plants endemic to Spain 252
16 3 Cryopreservation and the conservation of endangered endemic
Spanish plants 254
16 3 1 Cryopreservation of orthodox seeds 254
16 3 2 Cryopreservation of vegetatively propagated germplasm 257
16 4 Stability assessments 257
16 5 Plant diversity assessment for endangered species conservation 259
16 6 Conclusions 260
Acknowledgements 261
References 262
17 Recalcitrant Seed Biotechnology Applications to Rain Forest
Conservation 265
M Marzalina and B Krishnapillay
17 1 Introduction 265
17 2 Seed characteristics 266
17 2 1 Orthodox seeds 266
17 2 2 Recalcitrant seeds 266
17 2 3 Intermediate seeds 267
17 3 Seed collection and handling 267
17 4 Seed storage 267
17 4 1 Short-term and mid-term storage methods 268
17 4 2 Long-term storage 268
17 5 Cryopreservation 269
17 5 1 Cryopreservation of whole seeds 269
17 5 2 Cryopreservation of excised embryos 271
17 5 3 Cryopreservation of shoot tips 273
17 5 4 Cryopreservation of somatic embryos 273
17 6 Conclusions 274
References 274
18 Applications of Biotechnology for the Conservation and Sustainable
Exploitation of Plants from Brazilian Rain Forests 277
Ana Maria Viana, Maria Cristina Mazza and Sinclair Mantell
18 1 Introduction 277
18 2 Background 278
18 2 1 The Brazilian forests 278
18 3 Why are Brazilian forests endangered? 280
18 3 1 Abiotic factors 281
18 3 2 Biotic factors 281
xii Contents
18 4 Endangered tree species of the Brazilian forests 281
18 5 Current and potential uses of biotechnology for in situ and ex situ
conservation management of Brazilian forest species 283
18 5 1 Defining genetic diversity and differences between
populations of flora and fauna 283
18 5 2 Uses of in vitro culture techniques for propagation and
conservation 291
18 6 Conclusions and future prospects 294
Acknowledgements 295
References 295
Index 301
|
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spelling | Plant conservation biotechnology ed. by Erica E. Benson London [u.a.] Taylor & Francis 1999 XXV, 309 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biotechnologie gtt Natuurbehoud gtt Planten gtt Plantes - Biotechnologie ram Plantes - Conservation ram Plantes - Ressources génétiques ram Biotechnology methods Cryopreservation Germplasm resources, Plant Plant biotechnology Plants genetics Genetische Ressourcen (DE-588)4156623-3 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf Pflanzen (DE-588)4045539-7 s Genetische Ressourcen (DE-588)4156623-3 s Biotechnologie (DE-588)4069491-4 s DE-604 Benson, Erica E. Sonstige oth HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008755775&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
spellingShingle | Plant conservation biotechnology Biotechnologie gtt Natuurbehoud gtt Planten gtt Plantes - Biotechnologie ram Plantes - Conservation ram Plantes - Ressources génétiques ram Biotechnology methods Cryopreservation Germplasm resources, Plant Plant biotechnology Plants genetics Genetische Ressourcen (DE-588)4156623-3 gnd Biotechnologie (DE-588)4069491-4 gnd Pflanzen (DE-588)4045539-7 gnd |
subject_GND | (DE-588)4156623-3 (DE-588)4069491-4 (DE-588)4045539-7 |
title | Plant conservation biotechnology |
title_auth | Plant conservation biotechnology |
title_exact_search | Plant conservation biotechnology |
title_full | Plant conservation biotechnology ed. by Erica E. Benson |
title_fullStr | Plant conservation biotechnology ed. by Erica E. Benson |
title_full_unstemmed | Plant conservation biotechnology ed. by Erica E. Benson |
title_short | Plant conservation biotechnology |
title_sort | plant conservation biotechnology |
topic | Biotechnologie gtt Natuurbehoud gtt Planten gtt Plantes - Biotechnologie ram Plantes - Conservation ram Plantes - Ressources génétiques ram Biotechnology methods Cryopreservation Germplasm resources, Plant Plant biotechnology Plants genetics Genetische Ressourcen (DE-588)4156623-3 gnd Biotechnologie (DE-588)4069491-4 gnd Pflanzen (DE-588)4045539-7 gnd |
topic_facet | Biotechnologie Natuurbehoud Planten Plantes - Biotechnologie Plantes - Conservation Plantes - Ressources génétiques Biotechnology methods Cryopreservation Germplasm resources, Plant Plant biotechnology Plants genetics Genetische Ressourcen Pflanzen |
url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008755775&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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