Identification of molecular and structural components involved in gene silencing and recombination processes in plants:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
1999
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 171 S. Ill., graph. Darst. |
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| 100 | 1 | |a Müller, Andreas |d 1966- |e Verfasser |0 (DE-588)121199185 |4 aut | |
| 245 | 1 | 0 | |a Identification of molecular and structural components involved in gene silencing and recombination processes in plants |c vorgelegt von Andreas Müller |
| 264 | 1 | |c 1999 | |
| 300 | |a 171 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Köln, Univ., Diss., 1998 | ||
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Datensatz im Suchindex
| _version_ | 1807229531879112704 |
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| adam_text |
TABLE
OF
CONTENTS
1.
INTRODUCTION
1
1.1.
REGULATION
OF
GENE
EXPRESSION
1
1.2.
TRANSGENE
TECHNOLOGY
2
1.3.
GENE
SILENCING
IN
PLANTS
2
1.3.1.
TRANSCRIPTIONAL
SILENCING
3
1.3.2.
POST-TRANSCRIPTIONAL
SILENCING
8
1.4.
DNA
RECOMBINATION
9
1.4.1.
HOMOLOGOUS
RECOMBINATION
10
1.4.2.
ILLEGITIMATE
RECOMBINATION
11
1.5.
EXPERIMENTAL
SYSTEMS
13
2.
MATERIALS
AND
METHODS
17
2.1.
MATERIALS
17
2.1.1.
PLANT
MATERIAL
/
EUKARYOTIC
DNA
17
2.1.2.
PLANT
CDNA
LIBRARIES
17
2.1.3.
VECTORS
17
2.1.4.
BACTERIAL
STRAINS
18
2.2.
METHODS
18
2.2.1.
DNA
ANALYSIS
AND
CLONING
TECHNIQUES
18
2.2.1.1.
ISOLATION
OF
GENOMIC
DNA
FROM
PLANTS
18
2.2.1.2.
ISOLATION
OF
PLASMID
DNA
FROM
BACTERIA
19
2.2.1.3.
ISOLATION
OF
BACTERIOPHAGE
DNA
19
2.2.1.4.
GEL
ELECTROPHORESIS
OF
DNA
20
2.2.I.5.
FRAGMENTATION
OF
DNA
20
2.2.1.6.
ISOLATION
OF
DNA
FRAGMENTS
20
2.2.1.7.
CONSTRUCTION
OF
PLASMIDS
21
2.2.I.8.
TRANSFORMATION
OF
BACTERIA
21
2.2.1.9.
POLYMERASE
CHAIN
REACTION
(PCR)
21
2.2.1.10.
DNA
SEQUENCING
22
2.2.1.11.
RAPID
AMPLIFICATION
OF
CDNA
ENDS
(RACE)
23
2.2.1.12.
SOUTHERN
BLOT
HYBRIDISATION
23
2.2.1.13.
FLUORESCENCE
IN
SITU
HYBRIDISATION
(FISH)
23
2.2.1.14.
ELECTRON
MICROSCOPY
24
2.2.2.
RNA
ANALYSIS
25
2.2.2.I.
ISOLATION
OF
RNA
FROM
PLANTS
25
2.2.2.2.
GEL
ELECTROPHORESIS
OF
RNA
25
2.2.2.3.
NORTHERN
BLOT
HYBRIDISATION
26
2.2.3.
ANALYSIS
OF
DNA-PROTEIN
INTERACTIONS
AND
PROTEIN
PURIFICATION
26
2.2.3.1.
SOUTH-WESTERN
SCREENING
26
2.2.3.2.
PREPARATION
OF
PROTEIN
EXTRACTS
CONTAINING
FUSION
PROTEINS
28
2.2.3.3.
PROTEIN
PURIFICATION
28
2.2.3.4.
SDS-POLYACRYLAMIDE
GEL
ELECTROPHORESIS
(SDS-PAGE)
29
2.2.3.5.
ELECTROPHORETIC
MOBILITY
SHIFT
ANALYSIS
(EMSA)
29
2.2.4.
PLANT
TISSUE
CULTURE
AND
ANALYSIS
OF
TRANSGENIC
PLANTS
30
2.2.4.I.
PLANT
TRANSFORMATION
AND
REGENERATION
30
2.2.4.2.
GUS
ANALYSIS
32
2.2.4.3.
GFP
ANALYSIS
32
2.2.4.4.
LUCIFERASE
ANALYSIS
32
3.
RESULTS
33
3.1.
CHARACTERISATION
OF
RPS
33
3.1.1.
INFLUENCE
OF
RPS
ON
GUS
MARKER
GENE
ACTIVITY
IN
TRANSGENIC
TOBACCO
33
3.1.2.
PRESERVATION
OF
SEQUENCE
INTEGRITY
AT
A
DE
NOVO
METHYLATION
SITE
WITHIN
RPS
IN
TRANSGENIC
TOBACCO
35
3.1.3.
ENDOGENOUS
COPY
NUMBERS
OF
RPS
IN
PETUNIA
AND
TOBACCO
36
3.1.4.
DNA
METHYLATION
STATUS
OF
ENDOGENOUS
RPS-HOMOLOGUES
37
3.1.5.
DISTRIBUTION
OF
GENOMIC
REPETITIVENESS
WITHIN
THE
RPS
38
3.1.6.
NORTHERN
ANALYSIS
OF
RPS
40
3.1.7.
SEQUENCE
ANALYSIS
OF
RPS
40
3.1.8.
ELECTRON
MICROSCOPY
OF
RPS
45
3.1.9.
CHROMOSOMAL
LOCALISATION
OF
RPS^BLE-STEM
USING
FLUORESCENCE
IN
SITU
HYBRIDISATION
45
3.1.10.
INTERSPECIFIC
DISTRIBUTION
OF
RPS
51
3.2.
IDENTIFICATION
AND
CHARACTERISATION
OF
RPS-BINDING
PROTEINS
54
3.2.1.
IDENTIFICATION
OF
RPS-BINDING
PROTEINS
BY
SOUTH-WESTERN
SCREENING
54
3.2.2.
SEQUENCE
ANALYSIS
OF
CDNA
INSERTS
57
3.2.3.
ELECTROPHORETIC
MOBILITY
SHIFT
ANALYSIS
59
3.2.4.
PURIFICATION
OF
THE
ARA-I
FUSION
PROTEIN
68
3.2.5.
CLONING
OF
THE
FALL-LENGTH
CDNA
OF
ARA-I
BY
RAPID
70
AMPLIFICATION
OF
CDNA
ENDS
3.2.6.
EXPRESSION
ANALYSIS
OF
RPS-BPI
78
3.2.7.
INTERSPECIFIC
DISTRIBUTION
OF
RPS-BPI
80
3.2.8.
EXPRESSION
STUDIES
OF
RPS-BPI
IN
TRANSGENIC
PLANTS
81
3.3.
PALINDROMIC
DNA
ELEMENTS
IN
RECOMBINATION
PROCESSES
90
3.3.1.
PCR-AND
SOUTHERN
BLOT-ANALYSIS
OF
KANAMYCIN-RESISTANT
PLANTS
90
3.3.2.
SEQUENCE
ANALYSIS
OF
RECOMBINATION
PRODUCTS
92
4.
DISCUSSION
101
4.1.
RPS-INDUCED
GENE
SILENCING
101
4.1.1.
INITIATION
OF
THE
INACTIVE
STATE
103
4.1.2.
PROPAGATION
OF
THE
INACTIVE
STATE
111
4.1.3.
CHROMOSOMAL
LOCALISATION
OF
RPSDOUBLE-STEM
114
4.1.4.
RPS-BINDING
PROTEIN
I
115
4.2.
RECOMBINATION
AT
PALINDROMIC
DNA
SEQUENCE
ELEMENTS
131
4.2.1.
POSSIBLE
EVOLUTION
OF
RPS
REPEAT
UNITS
131
4.2.2.
RECOMBINATION
OF
PALINDROMIC
ELEMENTS
AND
132
A/T-RICH
/
DNA-UNWINDING
ELEMENTS
4.2.3.
RECOMBINATION
MODELS
136
5.
SUMMARY
140
6.
REFERENCES
143
ZUSAMMENFASSUNG
168 |
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| author_GND | (DE-588)121199185 |
| author_facet | Müller, Andreas 1966- |
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| id | DE-604.BV012747658 |
| illustrated | Illustrated |
| indexdate | 2024-08-13T00:19:47Z |
| institution | BVB |
| language | German |
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| owner_facet | DE-355 DE-BY-UBR DE-188 |
| physical | 171 S. Ill., graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
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| spelling | Müller, Andreas 1966- Verfasser (DE-588)121199185 aut Identification of molecular and structural components involved in gene silencing and recombination processes in plants vorgelegt von Andreas Müller 1999 171 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Köln, Univ., Diss., 1998 Rekombination (DE-588)4049338-6 gnd rswk-swf Inaktivierung (DE-588)4284643-2 gnd rswk-swf Petunie (DE-588)4173969-3 gnd rswk-swf Gen (DE-588)4128987-0 gnd rswk-swf DNS (DE-588)4070512-2 gnd rswk-swf Gartenpetunie (DE-588)4487260-4 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Gartenpetunie (DE-588)4487260-4 s Gen (DE-588)4128987-0 s Inaktivierung (DE-588)4284643-2 s DE-604 Petunie (DE-588)4173969-3 s DNS (DE-588)4070512-2 s Rekombination (DE-588)4049338-6 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008668817&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Müller, Andreas 1966- Identification of molecular and structural components involved in gene silencing and recombination processes in plants Rekombination (DE-588)4049338-6 gnd Inaktivierung (DE-588)4284643-2 gnd Petunie (DE-588)4173969-3 gnd Gen (DE-588)4128987-0 gnd DNS (DE-588)4070512-2 gnd Gartenpetunie (DE-588)4487260-4 gnd |
| subject_GND | (DE-588)4049338-6 (DE-588)4284643-2 (DE-588)4173969-3 (DE-588)4128987-0 (DE-588)4070512-2 (DE-588)4487260-4 (DE-588)4113937-9 |
| title | Identification of molecular and structural components involved in gene silencing and recombination processes in plants |
| title_auth | Identification of molecular and structural components involved in gene silencing and recombination processes in plants |
| title_exact_search | Identification of molecular and structural components involved in gene silencing and recombination processes in plants |
| title_full | Identification of molecular and structural components involved in gene silencing and recombination processes in plants vorgelegt von Andreas Müller |
| title_fullStr | Identification of molecular and structural components involved in gene silencing and recombination processes in plants vorgelegt von Andreas Müller |
| title_full_unstemmed | Identification of molecular and structural components involved in gene silencing and recombination processes in plants vorgelegt von Andreas Müller |
| title_short | Identification of molecular and structural components involved in gene silencing and recombination processes in plants |
| title_sort | identification of molecular and structural components involved in gene silencing and recombination processes in plants |
| topic | Rekombination (DE-588)4049338-6 gnd Inaktivierung (DE-588)4284643-2 gnd Petunie (DE-588)4173969-3 gnd Gen (DE-588)4128987-0 gnd DNS (DE-588)4070512-2 gnd Gartenpetunie (DE-588)4487260-4 gnd |
| topic_facet | Rekombination Inaktivierung Petunie Gen DNS Gartenpetunie Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008668817&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mullerandreas identificationofmolecularandstructuralcomponentsinvolvedingenesilencingandrecombinationprocessesinplants |