In vivo carbon-13 NMR:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Kluwer Acad./Plenum Publ.
1998
|
| Schriftenreihe: | Biological magnetic resonance
15 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Einzelaufnahme eines Zs.-Bandes |
| Beschreibung: | XV, 255 S. graph. Darst. |
| ISBN: | 0306458861 |
Internformat
MARC
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| 245 | 1 | 0 | |a In vivo carbon-13 NMR |c ed. by Lawrence J. Berliner ... |
| 264 | 1 | |a New York [u.a.] |b Kluwer Acad./Plenum Publ. |c 1998 | |
| 300 | |a XV, 255 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Biological magnetic resonance |v 15 | |
| 500 | |a Einzelaufnahme eines Zs.-Bandes | ||
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| 650 | 0 | 7 | |a Biologie |0 (DE-588)4006851-1 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1804127407959965696 |
|---|---|
| adam_text | Contents
Chapter 1
Tracer Theory and 13C NMR
Maren R. Laughlin and Joanne K. Kelleher
1. Introduction 1
i 1.1. Overview 1
j 1.2. Definitions 3
j 2. Characteristics of a Perfect Tracer 9
• 3. Compartmental Models 11
3.1. Introduction 11
3.2. Objectives and Identifiability 11
3.3. Parameter Estimation and Goodness of Fit 12
3.4. Linearity and Tracer Models 13
4. The Basic Tracer Experiment 14
4.1. General Considerations 14
4.2. Single Pool Model 16
4.3. Multicompartmental Catenary Model 18
5. Saturable Kinetic Processes 24
6. Condensation Reactions 25
7. Tissue Heterogeneity 27
7.1. Metabolic and Isotopic Steady State,
Time Dependent Experiment 28
7.2. Tissue Heterogeneity Measured in Pre Isotopic
Steady State 30
7.3. Fractional Enrichment in the Metabolic and Isotopic
Steady State Experiment 33
8. The 13C NMR Experiment 35
8.1. Chemical Shift and Spectral Resolution 35
xii Contents
8.2. Metabolic Perturbation 36
8.3. Detection Limits 36
8.4. Correction for Natural Abundance Fractional Enrichment. . . 37
8.5. Subtraction of Natural Abundance Spectra in
in Vivo Experiments 40
8.6. Sites of Label Entry and Sampling, and
Substrate Enrichment 40
8.7. Fractional Enrichment 45
8.8. Sensitivity and Time 49
8.9. Use of Reporter Molecules 51
9. Conclusions 55
References 56
Chapter 2
13C Isotopomer Analysis of Glutamate: A NMR Method to Probe
Metabolic Pathways Intersecting in the Citric Acid Cycle
A. Dean Sherry and Craig R. Malloy
1. Introduction 59
1.1. The Role of the Citric Acid Cycle in
Substrate Oxidation 60
1.2. Anaplerotic Functions of the Citric Acid Cycle 62
2. Quantitation of Glutamate Isotopomers by 13C NMR 63
2.1. Relation of Glutamate Isotopomers to Multiplets in the
13C NMR Spectrum 64
2.2. Acquisition of the 13C NMR Spectrum 65
2.3. Quantitation of 13C Fractional Enrichment by H NMR . . 65
2.4. Influence of Natural Abundance 13C on an
Isotopomer Analysis 67
3. Mathematical and Computer Models: Applications for
Isotopomer Analysis 67
3.1. The Value of Mathematical Models 68
3.2. Historical Background of Current Modeling Techniques . . 68
4. The Evolution of 13C Isotopomers in the Citric Acid Cycle .... 71
5. The Steady State 13C Isotopomer Analysis 75
6. The Nonsteady State Analysis 81
7. The Direct C4 Analysis: A Readout of Relative
Substrate Utilization 84
8. Steady State Analysis under Nonsteady State Conditions 87
9. Absolute Metabolic Fluxes from 13C Isotopomer Data 88
10. Other Considerations 94
References 95
Contents xiii
Chapter 3
Determination of Metabolic Fluxes by Mathematical Analysis of
13C Labeling Kinetics
John C. Chatham and Edwin M. Chance
1. Introduction 99
2. Approach to Analyzing Labeling Kinetics 101
3. Formulation of Model 102
4. Numerical Methods 108
5. Results and Discussion 110
6. Conclusions 113
References 115
Chapter 4
Metabolic Flux and Subcellular Transport of Metabolites
E. Douglas Lewandowski
1. Introduction 117
2. The General Utility of Dynamic Mode 13C NMR:
Lessons from the Heart 118
3. 13C NMR and Metabolic Activity 123
4. 13C Enrichment Patterns and Oxidative Metabolism 126
5. Fractional Enrichment and 13C NMR of Metabolic Flux 131
6. Models and Parameters of Glutamate Enrichment 134
7. Direct Kinetic Analysis of Dynamic 13C NMR Spectra 137
8. Metabolic Flux and Regulation from Dynamic
13C NMR Spectroscopy 146
9. Metabolite Compartmentation Effects on
13C Kinetics 149
10. 13C NMR of Subcellular Transport Rates 152
11. Summary 156
References 157
Chapter 5
Assessing Cardiac Metabolic Rates during Pathologic Conditions with
Dynamic 13C NMR Spectra
Robert G. Weiss and Gary Gerstenblith
1. Introduction 161
xiv Contents
2. Dynamic 13C NMR Spectroscopy in Paradigms of Myocardial
Dysfunction: Stunned and Hibernating Myocardium 162
2.1. Stunned Myocardium 162
2.2. Hibernating Myocardium 166
2.3. Implications for Distinguishing Types of Dysfunctional
Myocardium in the Clinical Setting: In Vivo 13C NMR ... 167
2.4. A Strategy for Measuring TCA Cycle Flux with
13CNMR 169
3. Dynamic 13C NMR Spectroscopy of Glycolysis in
Ischemic Preconditioning 172
3.1. Metabolic Changes in Ischemic Preconditioned Hearts . . . 172
3.2. Attenuated Ischemic Acidosis in Preconditioned Hearts . . 173
4. Summary 178
References 179
Chapter 6
Applications of 13C Labeling to Studies of Human Brain Metabolism in Vivo
Graeme F. Mason
1. Introduction 181
2. Measurement of the TCA Cycle Rate in the Brain 183
2.1. A General Description 183
2.2. Theoretical Basis of the Kinetic Modeling: Mass and
Isotope Balance 186
2.3. Overview of Interpretation of the Data by Mathematical
Modeling 187
2.4. Methods of Detection Currently in Use for Metabolic
Studies of Brain in Vivo 190
3. Mathematical Modeling: A Detailed Discussion 192
3.1. Description of Metabolic Flow 192
3.2. Equations and Procedures Used to Determine
Model Parameters 193
3.3. Measurement of Glutamate Turnover Rate (V t) 194
3.4. Determination of Vx/Vtca and Vtca from Vgt and C4/C3
Labeling Time Courses 194
3.5. Determination of Glutamate/Glutamine Carbon Flow (V ,n) 195
4. Derivation of Secondary Parameters 197
4.1. Estimation of Carbon Sources for the TCA Cycle 198
4.2. Determination of CMRgl 199
Contents xv
4.3. Brain Oxygen Consumption 199
5. Quantitative Analyses of Sensitivities 199
5.1. Glucose Turnover Time 199
5.2. Exchange of Lactate and Pyruvate 201
5.3. Effects of Metabolic Intermediates and Aspartate 201
5.4. Rates of Influx and Efflux of Lactate and Pyruvate 203
5.5. Exchange Between o Ketoglutarate and Glutamate 203
5.6. Pyruvate Carboxylase 203
5.7. Pentose Phosphate Shunt 205
5.8. Glucose Label Scrambling 205
5.9. Glutamate/Glutamine Carbon Flow 206
5.10. Summary of Sensitivity Analysis 207
6. Metabolic Compartmentation 207
6.1. Neuronal Activity and Compartmentation 207
6.2. Effects of Glutamate/Glutamine Compartmentation on
Measured Vtca 208
7. Future of 13C Labeling Studies of the Brain in Vivo 210
References 211
Chapter 7
In Vivo 13C NMR Spectroscopy: A Unique Approach in the Dynamic
Analysis of Tricarboxylic Acid Cycle Flux and Substrate Selection
Pierre Marie Luc Robitaille
1. Introduction 215
2. The Interaction between Major Biochemical
Pathways and Reactions 218
2.1. The Myoglobin System 218
2.2. The Lactate and Alanine Systems 219
2.3. The Creatine Kinase System 221
3. The History of Cellular 13C NMR 223
4. Post Steady State Analysis 224
4.1. The Theory of Post Steady State Analysis 226
5. In Vivo nC NMR Analysis of Substrate Selection 230
6. Conclusions 235
References 237
Contents of Previous Volumes 239
Index 253
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| id | DE-604.BV012740155 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:32:52Z |
| institution | BVB |
| isbn | 0306458861 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008663354 |
| oclc_num | 231787757 |
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| owner | DE-12 |
| owner_facet | DE-12 |
| physical | XV, 255 S. graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
| publisher | Kluwer Acad./Plenum Publ. |
| record_format | marc |
| series2 | Biological magnetic resonance |
| spelling | In vivo carbon-13 NMR ed. by Lawrence J. Berliner ... New York [u.a.] Kluwer Acad./Plenum Publ. 1998 XV, 255 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biological magnetic resonance 15 Einzelaufnahme eines Zs.-Bandes NMR-Spektroskopie (DE-588)4075421-2 gnd rswk-swf Biologie (DE-588)4006851-1 gnd rswk-swf Kohlenstoff-13 (DE-588)4164572-8 gnd rswk-swf Kohlenstoff-13 (DE-588)4164572-8 s NMR-Spektroskopie (DE-588)4075421-2 s Biologie (DE-588)4006851-1 s DE-604 Berliner, Lawrence J. Sonstige oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008663354&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | In vivo carbon-13 NMR NMR-Spektroskopie (DE-588)4075421-2 gnd Biologie (DE-588)4006851-1 gnd Kohlenstoff-13 (DE-588)4164572-8 gnd |
| subject_GND | (DE-588)4075421-2 (DE-588)4006851-1 (DE-588)4164572-8 |
| title | In vivo carbon-13 NMR |
| title_auth | In vivo carbon-13 NMR |
| title_exact_search | In vivo carbon-13 NMR |
| title_full | In vivo carbon-13 NMR ed. by Lawrence J. Berliner ... |
| title_fullStr | In vivo carbon-13 NMR ed. by Lawrence J. Berliner ... |
| title_full_unstemmed | In vivo carbon-13 NMR ed. by Lawrence J. Berliner ... |
| title_short | In vivo carbon-13 NMR |
| title_sort | in vivo carbon 13 nmr |
| topic | NMR-Spektroskopie (DE-588)4075421-2 gnd Biologie (DE-588)4006851-1 gnd Kohlenstoff-13 (DE-588)4164572-8 gnd |
| topic_facet | NMR-Spektroskopie Biologie Kohlenstoff-13 |
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