A guide to practical HPLC:
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
Darmstadt
GIT-Verl.
1999
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | 451 S. Ill., graph. Darst. |
| ISBN: | 3928865277 |
Internformat
MARC
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| 016 | 7 | |a 956878709 |2 DE-101 | |
| 020 | |a 3928865277 |c kart. : DM 90.00 |9 3-928865-27-7 | ||
| 035 | |a (OCoLC)76002092 | ||
| 035 | |a (DE-599)BVBBV012708774 | ||
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| 044 | |a gw |c DE | ||
| 049 | |a DE-20 | ||
| 084 | |a VG 7500 |0 (DE-625)147206:253 |2 rvk | ||
| 100 | 1 | |a Unger, Klaus K. |d 1936-2020 |e Verfasser |0 (DE-588)121177246 |4 aut | |
| 245 | 1 | 0 | |a A guide to practical HPLC |c K. K. Unger ; E. Weber |
| 264 | 1 | |a Darmstadt |b GIT-Verl. |c 1999 | |
| 300 | |a 451 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Literaturangaben | ||
| 650 | 0 | 7 | |a HPLC |0 (DE-588)4072641-1 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a HPLC |0 (DE-588)4072641-1 |D s |
| 689 | 0 | |5 DE-604 | |
| 700 | 1 | |a Weber, Eberhard |e Verfasser |4 aut | |
| 856 | 4 | 2 | |m DNB Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008638810&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-008638810 | |
Datensatz im Suchindex
| _version_ | 1807772999653261312 |
|---|---|
| adam_text |
CONTENTS
1
INTRODUCTION
.
1
1.1
SEPARATION
MODES
IN
CHROMATOGRAPHY.
1
1.2
HIGH
PERFORMANCE
LIQUID
CHROMATOGRAPHY
.
1
1.3
THE
HPLC
SYSTEM
.
2
1.4
SEPARATION
CRITERIA
IN
HPLC
.
2
1.5
REFERENCES
.
4
2
BASIC
PRINCIPLES
.
5
2.1
SEPARATION
PRINCIPLES
IN
HPLC
.
5
2.1.
I
THE
SEPARATION
COLUMN
.
5
2.1
2
TRANSPORT
OF
SOLUTES
THROUGH
THE
COLUMN
.6
2.1.3
SEPARATION
PRINCIPLES:
SELECTIVE
DILUTION
.
7
2.2
CHROMATOGRAPHIC
PARAMETERS
AND
CHARACTERISATION
OF
CHROMATOGRAPHIC
SEPARATIONS
.
.
9
2.3
ELUTION
MODES
IN
HPLC
.
13
2.3.1
ISOCRATIC
ELUTION
.
14
2.3
2
GRADIENT
ELUTION
.
14
3
METHODOLOGY
AND
INSTRUMENTATION
.
17
3.1
THE
HPLC
SYSTEM
.
17
3.1.1
COMPONENTS
OF
AN
HPLC
SYSTEM.
.
.
17
3.1.2
PUMPS
.
18
3.1.3
INJECTION
VALVES
.
22
3.1.4
CHROMATOGRAPHIC
COLUMNS
.
24
3.1.4.1
ANALYTICAL
COLUMNS
.
24
3.1.4.2
PREPARATIVE
COLUMNS
.
24
3.1.4.3
GUARD
COLUMNS
.
24
3.1.4.4
DESIGN
OF
AN
HPLC
COLUMN
.
25
3
1.5
DETECTORS
.
30
3.1
6
DATA
HANDLING
.
30
3.1.7
HPLC
SYSTEMS
.31
3.1.7.1
HPLC
SYSTEMS
FOR
METHOD
DEVELOPMENT
.
31
3.1.7.2
HPLC
SYSTEM
FOR
ROUTINE
ANALYSIS
.
31
3.2
MOBILE
PHASES
.
32
3.2.1
DEFINITION
AND
FUNCTION
OF
MOBILE
PHASES
IN
HPLC
.
32
3.2.2
CLASSIFICATION
OF
SOLVENTS.
THE
ELUOTROPIC
SERIES
.
32
3.2.3
PURITY
REQUIREMENTS
.
34
3.2.4
GENERAL
CRITERIA
FOR
CHOICE
OF
A
SOLVENT
.
36
3.2.5
PURIFICATION
OF
SOLVENTS
.
40
3.2.6
MOBILE
PHASES
FOR
REVERSED-PHASE
CHROMATOGRAPHY
.
41
3.2.7
MOBILE
PHASES
FOR
HPLC
ON
SILICA
AND
POLAR
CHEMICALLY
BONDED
SILICA.
43
3.3
PACKING
MATERIALS
.
44
3.3.1
CHARACTERISTICS
OF
PACKING
MATERIALS
.
44
3.3.2
COMPOSITION
OF
PACKING
MATERIALS
AND
STATIONARY
PHASES
.
47
3.3.2.1
CLASSIFICATION
OF
STATIONARY
PHASES
AND
ANALYTES
.
49
3.4
PHASE
SYSTEMS
.
55
3.4.1
PHASE
SYSTEMS
IN
HPLC
.
55
3.4.2
CHROMATOGRAPHY
ON
SILICA
AND
ALUMINA
(NORMAL-PHASE
CHROMATOGRAPHY)
.
59
3.4.3
CHROMATOGRAPHY
ON
NON-POLAR
STATIONARY
PHASES
(REVERSED
PHASE
CHROMATOGRAPHY)
.
60
3.4.4
CHROMATOGRAPHY
ON
NON-POLAR
STATIONARY
PHASES
USING
ION
PAIRING
AGENTS
(REVERSED-PHASE
ION-PAIR
CHROMATOGRAPHY)
.
.
62
3.4.5
CHROMATOGRAPHY
ON
POLAR,
CHEMICALLY
BONDED
SILICAS
.
65
3.4.6
CHROMATOGRAPHY
ON
ION-EXCHANGERS
(ION-EXCHANGE
CHROMATOGRAPHY)
.
66
3.4.7
SIZE-EXCLUSION
CHROMATOGRAPHY
.
.
68
3.5
USE
AND
CHARACTERISATION
OF
COLUMNS.
69
3.8.2.3
CALIBRATION
USING
AN
INTERNAL
STANDARD
.
113
3.5.1
CHOICE
OF
PARTICLE
SIZE
AND
COLUMN
DIMENSIONS
.
.
70
3.B.2.4
STANDARD
ADDITION
.
114
3.5.2
BAND
BROADENING
AND
SELECTION
OF
3
8.3
METHOD
VALIDATION
.
116
OPTIMUM
OPERATIONAL
PARAMETERS
.
.
75
3.8.3.1
SELECTIVITY
AND
SPECIFICITY
.
116
3.5.3
SAMPLE
INTRODUCTION
AND
3.8.3.2
MEASURING
OR
WORKING
RANGE
.
117
COLUMN
LOADABILITY
.
.
79
3.8.3.3
LINEARITY
.
117
3.5.4
DETERMINATION
OF
COLUMN
DEAD
TIME
.
80
3
8
3.4
SENSITIVITY
.
117
3.5.5
COLUMN
CARE
.
.
81
3.8.3.5
DETECTION
LIMIT
.
1
17
3.6
DEFECTION
.
.
84
3.8.3.6
LIMIT
OF
DETERMINATION
.
118
3.6.1
INTRODUCTION
.
.
84
3.B.3.7
PRECISION
AND
REPEATABILITY
.
118
3.6.2
UV
DETECTORS
.
.
86
3.8.3
8
ACCURACY
.
118
3.6.3
REFRACTIVE
INDEX
DETECTORS
.
.
92
3.8.39
RUGGEDNESS
.
119
3.6.4
FLUORESCENCE
DETECTORS
.
.
94
3.9
TESTING
HPLC
SYSTEMS
UNDER
119
GLP
CONDITIONS
.
3.6.5
ELECTROCHEMICAL
DEFECTORS
.
.
95
3.9.1
PRINCIPLES
OF
SYSTEM
TESTING
.
119
3.6.6
REACTION
DETECTION
.
.
98
3.9.2
DETERMINATION
OF
THE
TESTING
INTERVAL.
120
3.6
7
LIQUID
CHROMATOGRAPHY/
100
3.9.3
SYSTEM
TESTING
.
121
MASS
SPECTROMETRY
[LC/MS).
.
DATA
ACQUISITION,
EVALUATION
AND
3.9.3.1
TOTAL
SYSTEM
TESTS
AND
SINGLE
3.7
MODULE
TESTS
.
121
VALIDATION
.
101
3.9.3.2
EXAMPLES
AND
RECOMMENDATIONS
FOR
3.7.1
DATA
ACQUISITION
.
101
TOTAL-SYSTEM
TESTS
.
123
3.7.2
QUALITATIVE
DATA
ANALYSIS
.
101
3.9.3.3
SYSTEM-SUITABILITY
TEST
.
123
3.7.3
PEAK
AREA
AND
PEAK
HEIGHT
3.9
3.4
REPRODUCIBILITY
OF
INJECTION,
EVALUATION
.
102
PUMP
FLOW
AND
MOBILE
PHASE
3.7.4
MANUAL
QUANTITATION
.
102
GRADIENTS
.
125
3.7.5
QUANTITATION
USING
INTEGRATORS
AND
3.9.3.5
TEST
ON
SAMPLE
CARRY-OVER
.
126
DATA
SYSTEMS
.
103
3.9.3.6
TESTING
DETECTOR
SENSITIVITY
.
126
3.7.5.1
INTEGRATION
.
104
3.9.3.7
ACCURACY
OF
GRADIENT
FORMATION
AND
LINEARITY
OF
THE
UV
DETECTOR
.
.
127
3.7.5.2
PEAK
IDENTIFICATION
AND
PEAK
ASSIGNMENT
.
106
3.9.4
DOCUMENTATION
OF
TEST
RESULTS
ACCORDING
TO
GLP
GUIDELINES
.
.
128
3.7.5.3
ANALYSIS
REPORT
.
108
3.9.5
PROSPECTS
.
129
3.8
QUANTITATION
AND
VALIDATION
.
110
3.10
TROUBLESHOOTING
.
130
3.8.1
IDENTIFICATION
(QUALITATIVE
ANALYSIS)
.
110
3.10.1
INTRODUCTION
.
130
3.8.2
QUANTITATIVE
ANALYSIS
.
111
3.10.2
PREVENTION
OF
PROBLEMS
.
130
3.8.2.1
AREA
PERCENTAGE
MEASUREMENT
111
3.10.3
GENERAL
RECOMMENDATIONS
.
130
(NORMALISATION
METHOD)
.
3.8.2.2
CALIBRATION
USING
AN
3.10.4
STRATEGIES
FOR
TROUBLESHOOTING
.
137
EXTERNAL
STANDARD
.
1
12
3.11
REFERENCES
.
143
4
STRATEGIES
AND
SOLUTIONS
FOR
SEPARATION
PROBLEMS
145
4.1
STRATEGIES
FOR
METHOD
DEVELOPMENT
.
145
4.1.1
CHOOSING
A
CHROMATOGRAPHIC
METHOD
.
148
4.1.2
SURVEY
ON
METHOD
DEVELOPMENT
.
.
149
4.1.3
METHOD
DEVELOPMENT
FOR
REVERSED
PHASE
SEPARATIONS
.
150
4.1.4
METHOD
DEVELOPMENT
FOR
SEPARATION
ON
SILICA
(NORMAL-PHASE
CHROMATOGRAPHY)
.
165
4.1.5
OPTIMISATION
OF
METHODS
WITH
RESPECT
TO
SENSITIVITY,
ANALYSIS
TIME
AND
SELECTIVITY
.
167
4.1.6
REFERENCES
.
169
4.2
PRACTICAL
EXAMPLES
.
169
4.2.1
REVERSED
PHASE
CHROMATOGRAPHY
.
.
169
4.2.1.1
RPLC
1:
WATER
CONTENT
-
RESOLUTION
DEPENDING
ON
RETENTION
FACTOR
K
AND
COLUMN
LENGTH
.
169
4.2.1.2
RPLC
2
PH
VALUE
.
173
4.2.1.3
RPLC
3:
TERNARY
MIXTURE
.
177
4.2.1.4
RPLC
4:
COMPARISON
OF
C8/C
18
STATIONARY
PHASES
.
179
4.2.1.5
RPLC
5:
TEMPERATURE
.
182
4.2.1.6
RPLC
6
COMPARISON
BETWEEN
ISO
CRAFIC
AND
GRADIENT
ELUTION
.
184
4.2.1.7
RPLC
7:
GRADIENT
ELUTION:
CONDITIONS
USED
FOR
TRANSFERRING
GRADIENT
ELUTION
TO
THE
ISOCRATIC
MODE
.
187
4.2.1.8
RPLC
8:
GRADIENT
ELUTION,
CHOICE
OF
THE
CORRECT
GRADIENT
CONDITIONS.
194
4.2.1.9
RPLC
9'
TESTING
RP-COLUMNS
.
.198
4.2
1
10
RPLC
10:
FAST
RPLC
SEPARATIONS.
.
201
4
2.2
CHROMATOGRAPHY
ON
SILICA
(NORMAL
PHASE
CHROMATOGRAPHY)
.
208
4.2.2.1
NPLC
1.
STANDARDISATION
.
208
4.2.2.2
NPLC
2:
EFFECTS
OF
THE
SPECIFIC
SURFACE
AREA
OF
THE
PACKING
MATERIAL
ON
RETENTION
.
211
4.2.3
CHROMATOGRAPHY
ON
POLAR,
CHEMICALLY
BONDED
SILICAS
.
213
4.2.3.1
PBPC
1:
HYDROPHOBIC/HYDROPHILIC
CHARACTER
.
213
4.2.4
REVERSED
PHASE
ION-PAIR
CHROMATOGRAPHY
.
216
4.2.4.1
IPC1:
ACIDS
.
216
4.2.4.2
IPC
2:
BASES
.
219
4.2.5
ION-EXCHANGE
CHROMATOGRAPHY.
.
.
.
221
4.2.5.1
IEC1:
ANIONS
.
221
4.2.5.2
IEC
2:
DETERMINATION
OF
INORGANIC
ANIONS
AND
CATIONS
USING
CONDUCTIVITY
DETECTION
.
223
4.2
6
SIZE
EXCLUSION
CHROMATOGRAPHY
.
.
.
227
4.2.6.1
SEC
1:
SEPARATION
OF
POLYSTYRENES
.
227
4.2.6.2
SEC
2:
PROTEINS
.
230
5
SAMPLE
PREPARATION
.
233
5.1
INTRODUCTION
.
233
5.2
HOMOGENEOUS
SAMPLES
.
233
5.3
COMPLEX
MATRICES
.
235
5.4
PROCEDURES
FOR
SAMPLE
PRETREATMENF
238
5.4
1
SAMPLING
.
238
5.4.2
EXTRACTION
.
239
5.4.3
CLEAN-UP
METHODS
.
240
5.5
SAMPLE
CLEAN-UP
BY
LIQUID-SOLID
ADSORPTION
(OFF-LINE
SPE)
.
245
5.5.1
PROCEDURE
.
245
5.5.2
OPERATION
OF
SPE
COLUMNS
(CARTRIDGES)
.
247
5.5.3
METHOD
DEVELOPMENT
IN
LIQUID-SOLID
ADSORPTION
(SPE)
.
248
5.5.4
AUTOMATED
(ON-LINE)
SOLID
PHASE
EXTRACTION
(ON-LINE
SPE)
.
251
5.5.5
APPLICATION
EXAMPLES
OF
SOLID
PHASE
EXTRACTION
(SPE)
.
254
5.5.5.1
SOLID
PHASE
EXTRACTION
OF
FOLIC
ACID
FROM
DIET
FOOD
.
254
5.5.5.2
SOLID
PHASE
EXTRACTION
OF
ATRAZINE
FROM
CORN
OIL
.
256
5.5.5
3
SOLID
PHASE
EXTRACTION
OF
BENZODI
AZEPINES
FROM
SERUM
.
258
5.6
SPECIAL
TECHNIQUES
IN
SAMPLE
PREPARATION
.
260
5.6.1
COLUMN-SWITCHING
.
260
5.6.2
SPECIAL
SUPPORTS
FOR
SOLID
PHASE
EXTRACTION
.
262
5.7
DERIVATISATION
PROCEDURES
.
264
5.8
REFERENCES
.
266
6
APPLICATIONS
.
267
6.
1
PURITY
CONTROL
OF
INDUSTRIAL
PRODUCTS
AND
CHEMICALS
BY
HPLC
.
267
6.1.1
ANALYSIS
OF
DETERGENTS
.
267
6.1.2
DETERMINATION
OF
FREE
4,4'-DIPHENYL
METHANEDIISOCYANATE
IN
POLYURETHANE-PREPOLYMERS
.
.
.
272
6.1.3
PURITY
TESTING
OF
VITAMIN
E
.
275
6.1
4
DETERMINATION
OF
UV-ACTIVE
CONTAMINANTS
IN
FUMARIC
ACID
DI
METHYL
ESTER
AND
IN
FUMARIC
ACID
MONOETHYL
ESTER
CALCIUM
SALT
.
277
6.1.5
PURIFY
TESTING
AND
DETERMINATION
OF
THE
REACTION
BY-PRODUCTS
OF
L-AMINO
NAPHTHALENE-7-SULFONIC
ACID
.
.279
6.2
HPLC
IN
ENVIRONMENTAL
ANALYSIS
.
282
6.2.1
IDENTIFICATION
AND
DETERMINATION
OF
POLYCHLOROPHENOLS
IN
SURFACE
AND
WASTE
WATERS
.
283
6.2.2
DETERMINATION
OF
PHENOXYCARBOXYLIC
ACIDS
IN
AQUEOUS
SAMPLES
.
.
286
6.2.3
DETERMINATION
OF
POLYCYCLIC
AROMATIC
HYDROCARBONS
IN
SOIL
SAMPLES.
292
6.2.4
DETERMINATION
OF
AMITROL
RESIDUES
IN
PLANT
MATERIALS
.
297
6.2.5
DETERMINATION
OF
POLAR
PESTICIDES
IN
DRINKING
WATER
BY
SOLID
PHASE
EXTRACTION
AND
DIODE
ARRAY
DETECTION
.
300
6.2.6
DETERMINATION
OF
NITRO-COMPOUNDS
IN
MILITARY
HAZARDOUS
WASTE.
.
307
6.3
HPLC
IN
FOOD
ANALYSIS
.
312
6.3.1
DETERMINATION
OF
FAT
AND
WATER
SOLUBLE
VITAMINS
.
312
6.3.2
DETERMINATION
OF
CHLOROGENIC
ACIDS
IN
COFFEE
.
323
6.3.3
DETERMINATION
OF
CHLORAMPHENICOL
AND
SULPHONAMIDES
IN
MEAT.
.
326
6.3.4
DETERMINATION
OF
SUGARS
IN
DIET
DRINKS
.
329
6.3.5
DETERMINATION
OF
ANTIOXIDANTS
AND
PRESERVATIVES
IN
FOODS
.
330
6.3.6
DETERMINATION
OF
VITAMIN
B
2
IN
MILK.
334
6.4
PHARMACEUTICAL
ANALYSIS
.
336
6.4.1
DETERMINATION
OF
CONCENTRATION
AND
PURITY
TESTING
OF
HYDROCHLOROTHIAZIDE
.
340
6.4.2
PURITY
TESTING
OF
DIPYRIDAMOL
BY
HPLC
AND
COMPARISON
OF
THE
RESULTS
WITH
A
DOCUMENTED
TLC-METHOD
.
345
6.4.3
DETERMINATION
OF
AN
ENZYMATIC
HYDROLYSIS
PRODUCT
OF
PENICILLIN
G
.
348
6.4.4
DETERMINATION
AND
PURITY
TESTING
OF
CHLORDIAZEPOIDE
IN
SOLID
PHARMACEUTICAL
FORMULATIONS
.
350
6
4.5
DETERMINATION
AND
PURITY
TESTING
OF
DROPERIDOL
AND
THE
PRESERVATIVES
METHYL
AND
PROPYLPARABEN
AS
WELL
AS
THEIR
HYDROLYSIS
PRODUCTS
IN
LIQUID
PREPARATIONS
.
354
6.4.6
DETERMINATION
OF
THENOYLPEROXIDE
IN
SEMI-SOLID
PHARMACEUTICAL
FORMULATIONS
.
356
6.4.7
POST-COLUMN
DERIVATISATION
OF
AMINOGLYCOSIDE
ANTIBIOTICS
.
.
358
6.4.8
INFLUENCE
OF
COLUMN
TEMPERATURE
ON
THE
REVERSED
PHASE
SEPARATION
OF
DIHYDROERGOTOXIN-METHANE
SULFONATE
.
361
6.4.9
REFERENCES
FOR
6
4
6.5
HPLC
IN
CLINICAL
CHEMISTRY
.
364
6.5.1
DETERMINATION
OF
VITAMIN
A
IN
SERUM
.
366
6.5
2
DETERMINATION
OF
PORPHYRINS
IN
URINE.
368
6.5.3
DETERMINATION
OF
AMINO
ACIDS
IN
HUMAN
PLASMA
.
370
6.5.4
DETERMINATION
OF
ANTI-EPILEPTIC
DRUGS
AND
METABOLITES
IN
SERUM
376
6.5.5
DIRECT
AND
FULLY
AUTOMATED
DETERMINA
TION
OF
CATECHOLAMINES
NORADRENALINE
AND
ADRENALINE
IN
HUMAN
PLASMA
.
381
6.6
HPLC
IN
TOXICOLOGY
.
387
6.6.1
SCREENING
ANALYSIS
OF
STOMACH
CONTENT
.
387
6
6.2
PROFILING
ANALYSIS
IN
URINE
.
390
6
6.3
ANALYSIS
OF
DICLOFENAC
IN
URINE.
.
.
.
392
6.6.4
DETERMINATION
OF
COUMARINS
IN
SERUM
.
393
6.6.5
QUANTITATIVE
DETERMINATION
OF
BARBITURATES
IN
SERUM
.
395
6.6.6
QUANTITATIVE
DETERMINATION
OF
PARACETAMOL
IN
SERUM
.
397
6.6.7
REFERENCES
FOR
SECTION
6.6
.
398
6.7
ANALYSIS
OF
NATURAL
PRODUCTS
.
399
6.7.1
QUALITATIVE
ANALYSIS
OF
BIOLOGICALLY
ACTIVE
NAPHTHOQUINONS
IN
A
TRADITIONAL
AFRICAN
MEDICINAL
HERB
.
401
6.7.2
PRECOLUMN
DERIVATISATION
OF
NATURAL
PRODUCTS
TO
INCREASE
DETECTABILITY.
QUANTITATIVE
ANALYSIS
OF
THE
MOLLUSCICIDAL
TRITERPENE
SAPONINS
FROM
PHYTOLACCA
DODECANDRA
.
404
6.7.3
DETECTION
OF
A
COUNTERFEIT
MEDICINAL
PLANT
SUBSTITUTION
OF
THE
ROOT
DRUG
OF
ECHINACEA
PURPUREA
BY
PARTHENIUM
INTEGRIFOLIUM
.
407
6.7.4
STANDARDISATION
OF
PHYTOPHARMA
CEUTICALS
DETERMINATION
OF
SENNOSIDES
A
AND
8
IN
SENNA
PREPARATIONS
.
409
6.7
5
SAMPLE
PREPARATION
FOR
DETECTION
AT
LOW
WAVELENGTHS
DETERMINATION
OF
PHARMACOLOGICALLY
ACTIVE
DIFERPENES
FROM
MONTANOA
SPECIES
.
411
6.7.6
ON-LINE
AND
OFF-LINE
PEAK
IDENTIFICATION:
ANALYSIS
OF
HEPATOTOXIC
PYRROLIZIDINE
ALKALOIDS
IN
SENECIO
SPECIES
.414
6.7.7
REFERENCES
FOR
6.7
6.8
POLYMER
ANALYSIS
.
418
6.8.1
MOLECULAR
MASS
DETERMINATION
OF
AGAR-AGAR
BY
SEC
.
420
6.8.2
SEC
OF
PHENYLENE
OXIDE-STYRENE
COPOLYMERS
.
422
6.8.3
CHARACTERISATION
OF
SLYRENE-ACRYLATE
COPOLYMERS
USING
RI
AND
UV
DETECTION
.
424
6.8.4
MOLECULAR
MASS
DETERMINATION
OF
POLAR
POLYACRYLAMIDE
BY
SEC
.
427
6.8.5
SIMULTANEOUS
MOLECULAR
MASS
AND
MONOMER
CONTENT
DETERMINATION
OF
POLYESTERS
.
.
429
LIST
OF
TABLES
.
433
LIST
OF
FIGURES
.
435
INDEX
.
444 |
| any_adam_object | 1 |
| author | Unger, Klaus K. 1936-2020 Weber, Eberhard |
| author_GND | (DE-588)121177246 |
| author_facet | Unger, Klaus K. 1936-2020 Weber, Eberhard |
| author_role | aut aut |
| author_sort | Unger, Klaus K. 1936-2020 |
| author_variant | k k u kk kku e w ew |
| building | Verbundindex |
| bvnumber | BV012708774 |
| classification_rvk | VG 7500 |
| ctrlnum | (OCoLC)76002092 (DE-599)BVBBV012708774 |
| discipline | Chemie / Pharmazie |
| format | Book |
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| id | DE-604.BV012708774 |
| illustrated | Illustrated |
| indexdate | 2024-08-19T00:17:59Z |
| institution | BVB |
| isbn | 3928865277 |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008638810 |
| oclc_num | 76002092 |
| open_access_boolean | |
| owner | DE-20 |
| owner_facet | DE-20 |
| physical | 451 S. Ill., graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | GIT-Verl. |
| record_format | marc |
| spelling | Unger, Klaus K. 1936-2020 Verfasser (DE-588)121177246 aut A guide to practical HPLC K. K. Unger ; E. Weber Darmstadt GIT-Verl. 1999 451 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Literaturangaben HPLC (DE-588)4072641-1 gnd rswk-swf HPLC (DE-588)4072641-1 s DE-604 Weber, Eberhard Verfasser aut DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008638810&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Unger, Klaus K. 1936-2020 Weber, Eberhard A guide to practical HPLC HPLC (DE-588)4072641-1 gnd |
| subject_GND | (DE-588)4072641-1 |
| title | A guide to practical HPLC |
| title_auth | A guide to practical HPLC |
| title_exact_search | A guide to practical HPLC |
| title_full | A guide to practical HPLC K. K. Unger ; E. Weber |
| title_fullStr | A guide to practical HPLC K. K. Unger ; E. Weber |
| title_full_unstemmed | A guide to practical HPLC K. K. Unger ; E. Weber |
| title_short | A guide to practical HPLC |
| title_sort | a guide to practical hplc |
| topic | HPLC (DE-588)4072641-1 gnd |
| topic_facet | HPLC |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008638810&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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