Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German English |
| Veröffentlicht: |
1998
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Jena, Univ., Diss., 1998 |
| Beschreibung: | 85 Bl. Ill., graph. Darst. |
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| 245 | 1 | 0 | |a Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines |c von Michael Lehmann |
| 264 | 1 | |c 1998 | |
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Datensatz im Suchindex
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TABLE OF CONTENTS
ZUSAMMENFASSUNG 1
1. INTRODUCTION 3
2. MATERIAL AND METHODS 15
2.1. CELL CULTURE 15
2.2. STIMULATION OF CELLS 15
2.3. CHEMICALS 15
2.4. DETERMINATION OF IL-10 AND TNF-ALPHA PROTEIN CONCENTRATION 16
2.5. TOTAL RNA ISOLATION AND CDNA SYNTHESIS 17
2.6. CONSTRUCTION OF THE GAPDH COMPETITOR FRAGMENT 17
2.7. RT-PCR FOR QUANTIFICATION OF MRNA 19
2.8. PLASMID CONSTRUCTION 21
2.9. ELECTROPORATION 21
2.10. TRANSFECTION OF U937 CELLS 23
2.11. LUCIFERASE ASSAY 23
3. RESULTS 24
3.1. INVOLVEMENT OF PROTEIN KINASE A AND C IN IL-10 EXPRESSION 24
3.1.1. EL-10 PROTEIN PRODUCTION IS SYNERGISTICALLY INDUCED BY 24
ACTIVATION OF BOTH PROTEIN KINASE A AND C IN MONOCYTIC CELL LINES
3.1.2. THE IL-10 PROMOTER ACTIVITY IS ENHANCED BY BOTH ILOPROST AND 28
DIBUTYRYL-CAMP
3.2. ELECTROPORATION TRIGGERS IL-10 EXPRESSION IN THE MONOCYTIC CELL
LINES 30
U937 AND THP-1
3.2.1. PULSE STRENGTH AND PULSE DURATION DETERMINE LL-IO PROTEIN 30
PRODUCTION
3.2.2. TIME DEPENDENCE OF IL-10 PROTEIN PRODUCTION 32
3.2.3. SPECIFICITY OF THE TRIGGERED PROCESS 33
BIBLIOGRAFISCHE INFORMATIONEN
HTTP://D-NB.INFO/956674852
3.2.4. ELECTROPORATION-TRIGGERED IL-10 PROTEIN PRODUCTION IS ELEVATED 34
BY STIMULATION OF BOTH PROTEIN KINASE A AND C
3.2.5. SIGNAL TRANSDUCTION EVENTS INVOLVED IN ELECTROPORATION- 36
TRIGGERED IL-10 EXPRESSION
3.2.5.1. CALCIUM DEPENDENCE OF IL-10 EXPRESSION 36
3.2.5.2. CALMODULIN DEPENDENCE OF IL-10 EXPRESSION 39
3.2.5.3. ELECTROPORATION-TRIGGERED IL-10 EXPRESSION IS 43
INHIBITED BY BLOCKING OF THE CA
2+/CALMODULIN-DEPENDENT
PROTEIN KINASE SIGNAL TRANSDUCTION PATHWAY
3.3. IONOMYCIN INDUCES IL-10 EXPRESSION IN U937 CELLS 46
3.3.1. ELEVATION OF INTRACELLULAR CALCIUM LEVELS BY IONOMYCIN 46
INDUCES IL-10 MRNA TRANSCRIPTION
3.3.2. IL-10 MRNA TRANSCRIPTION IS BLOCKED BY THE CALMODULIN 47
ANTAGONIST W7
3.4. GM-CSF STIMULATES IL-10 EXPRESSION IN U937 CELLS 48
3.4.1. IL-10 PROTEIN PRODUCTION IS INDUCED BY GM-CSF 48
3.4.2. EL-10 MRNA TRANSCRIPTION IS INDUCED BY GM-CSF WITH PEAK 49
EXPRESSION AT 6 HOURS AFTER STIMULATION
3.4.3. IL-10 IS INDUCED BY GM-CSF INDEPENDENTLY FROM TNF-ALPHA 52
3.4.4. GM-CSF DIRECTS THE IL-10 PROMOTER 53
3.4.5. SIGNALLING EVENTS IN GM-CSF-INDUCED IL-10 EXPRESSION 53
4. DISCUSSION 56
5. PROPOSED MODEL OF IL-10 EXPRESSION IN MONOCYTES 68
6. ABBREVIATIONS 69
7. REFERENCES
70 |
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| indexdate | 2024-10-09T18:15:06Z |
| institution | BVB |
| language | German English |
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| physical | 85 Bl. Ill., graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
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| spelling | Lehmann, Michael 1966- Verfasser (DE-588)121167607 aut Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines von Michael Lehmann 1998 85 Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Jena, Univ., Diss., 1998 GM-CSF (DE-588)4304243-0 gnd rswk-swf Maus (DE-588)4169148-9 gnd rswk-swf Interleukin 10 (DE-588)4337389-6 gnd rswk-swf Elektroporation (DE-588)4237755-9 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Maus (DE-588)4169148-9 s GM-CSF (DE-588)4304243-0 s Interleukin 10 (DE-588)4337389-6 s Genexpression (DE-588)4020136-3 s DE-604 Elektroporation (DE-588)4237755-9 s DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008627804&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lehmann, Michael 1966- Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines GM-CSF (DE-588)4304243-0 gnd Maus (DE-588)4169148-9 gnd Interleukin 10 (DE-588)4337389-6 gnd Elektroporation (DE-588)4237755-9 gnd Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)4304243-0 (DE-588)4169148-9 (DE-588)4337389-6 (DE-588)4237755-9 (DE-588)4020136-3 (DE-588)4113937-9 |
| title | Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines |
| title_auth | Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines |
| title_exact_search | Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines |
| title_full | Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines von Michael Lehmann |
| title_fullStr | Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines von Michael Lehmann |
| title_full_unstemmed | Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines von Michael Lehmann |
| title_short | Influence of electroporation and GM-CSF on the expression of interleukin-10 in human monocytic cell lines |
| title_sort | influence of electroporation and gm csf on the expression of interleukin 10 in human monocytic cell lines |
| topic | GM-CSF (DE-588)4304243-0 gnd Maus (DE-588)4169148-9 gnd Interleukin 10 (DE-588)4337389-6 gnd Elektroporation (DE-588)4237755-9 gnd Genexpression (DE-588)4020136-3 gnd |
| topic_facet | GM-CSF Maus Interleukin 10 Elektroporation Genexpression Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008627804&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT lehmannmichael influenceofelectroporationandgmcsfontheexpressionofinterleukin10inhumanmonocyticcelllines |