Genome analysis: a laboratory manual 3 Cloning systems
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Plainview, NY
Cold Spring Harbor Laboratory Press
1999
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXXII, 648 S. graph. Darst. |
| ISBN: | 0879695137 |
Internformat
MARC
| LEADER | 00000nam a2200000 cc4500 | ||
|---|---|---|---|
| 001 | BV012675939 | ||
| 003 | DE-604 | ||
| 005 | 00000000000000.0 | ||
| 007 | t | ||
| 008 | 990722s1999 d||| |||| 00||| eng d | ||
| 020 | |a 0879695137 |9 0-87969-513-7 | ||
| 035 | |a (OCoLC)490398624 | ||
| 035 | |a (DE-599)BVBBV012675939 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-20 |a DE-11 |a DE-188 | ||
| 050 | 0 | |a QH445.2 | |
| 245 | 1 | 0 | |a Genome analysis |b a laboratory manual |n 3 |p Cloning systems |c series ed. Eric D. Green ... vol. eds. Bruce Birren ... |
| 264 | 1 | |a Plainview, NY |b Cold Spring Harbor Laboratory Press |c 1999 | |
| 300 | |a XXXII, 648 S. |b graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 7 | |a Genome Analysis |2 cabt | |
| 650 | 7 | |a ADN recombiné - Manuels de laboratoire |2 ram | |
| 650 | 7 | |a Chromosomes - Cartes - Technique |2 ram | |
| 650 | 7 | |a Clonage moléculaire - Manuels de laboratoire |2 ram | |
| 650 | 7 | |a Clonage |2 ram | |
| 650 | 7 | |a Séquence nucléotidique |2 ram | |
| 650 | 4 | |a Molecular cloning |x Laboratory manuals | |
| 650 | 4 | |a Recombinant DNA |x Laboratory manuals | |
| 700 | 1 | |a Green, Eric D. |e Sonstige |4 oth | |
| 700 | 1 | |a Birren, Bruce |e Sonstige |4 oth | |
| 773 | 0 | 8 | |w (DE-604)BV011728062 |g 3 |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008614805&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-008614805 | ||
Datensatz im Suchindex
| _version_ | 1804127336730198016 |
|---|---|
| adam_text | Contents
VOLUME 3
Companion Volumes, xv
Preface, xvii
Dedication, xxi
Acknowledgments, xxiii
Contributors, xxv
Abbreviations and Acronyms, xxvii
1 Bacterial Cloning Systems, 1
/. Dunham, K. Dewar, U. J. Kim, M.T. Ross
GENOMIC LIBRARIES, 2
CONSTRUCTION OF BACTERIAL GENOMIC LIBRARIES, 3
Characteristics of Different Bacterial Cloning Systems, 3
Average Insert Size and Representation of the Genome, 3
DNA Source, 6
Amount and Quality of DNA Required, 7
The Bacterial Host Strain, 7
PLATING, HANDLING, AND STORING LIBRARIES, 11
PROTOCOLS
Manual Library Arraying Protocol, 16
Manual Replication, 18
Robotic Replication, 20
Recovery of Individual Clones, 21
LIBRARY SCREENING, 22
PREPARATION OF FILTERS CONTAINING ARRAYED BACTERIAL CLONE
COLONIES, 23
PROTOCOLS
Generation of Filters Containing Low density Arrays of Bacterial Clone Colonies, 26
V
Vi Contents
Generation of Filters Containing High density Arrays of Bacterial Clone Colonies, 28
Lysis of Arrayed Bacterial Clone Colonies, 30
HYBRIDIZATION BASED SCREENING OF ARRAYED BACTERIAL CLONES, 32
PROTOCOLS
Hybridization of Filters Containing Arrayed Bacterial Clone Colonies, 36
Generation of Radiolabeled Probes from PCR Products, 39
PCR BASED SCREENING OF GENOMIC LIBRARIES, 41
PROTOCOLS
Preparation ofDNA Pools from Arrayed Bacterial Clone Colonies, 44
PCR Testing ofDNAfrom Pools of Bacterial Clones, 47
CONTIG ASSEMBLY AND CHROMOSOME WALKING, 49
PREPARATION OF DNA FROM CLONE CULTURES, 50
PROTOCOLS
Minipreparation of Cloned DNA from Individual Clone Cultures, 51
96 well Format Micropreparation of Clone DNA, 53
Large scale Alkaline Lysis DNA Preparation for Large insert Clones, 55
RESTRICTION ENZYME FINGERPRINT ANALYSIS OF BACTERIAL CLONES, 58
PROTOCOLS
Restriction Enzyme Fingerprinting Using Agarose Gel Electrophoresis, 61
Radioactive Method for Restriction Enzyme Digest Fingerprinting Using Hind/// and
Sau3Al, 63
Preparation of Markers, 65
Sflw3Al cut Bacteriophage X Markers, 65
Hinfl cut Bacteriophage A, Markers, 66
Polacrylamide Gel Preparation and Electrophoresis, 67
Radioactive Method for Restriction Enzyme Digest Fingerprinting Using Hind/// and
Msp/, 69
COMPUTER SOFTWARE FOR CONTIG ASSEMBLY, 71
CHROMOSOME WALKING, 72
RECOVERING END SEQUENCES OF BACTERIAL CLONES, 75
PROTOCOLS
Vector~ette End Rescue of Bacterial Clones, 79
Vectorette Library Construction, 79
Vectorette PCR Amplification, 80
Direct Sequencing of Large insert Bacterial Clones, 82
2 Cosmids, 87
G.A. Evans
GENERAL PRINCIPLES OF CLONING IN COSMIDS, 88
Advantages and Disadvantages of Cosmid Cloning Systems, 88
Uses and Applications of Cosmid Libraries, 88
COSMID VECTORS AND LIBRARY CONSTRUCTION, 90
Technical Considerations: Host Strains and Clone Instability, 90
Structure of Cosmid Vectors, 90
Outline of Genomic DNA Library Construction in Cosmid Vectors, 92
Contents VM
COSMID LIBRARY CHARACTERIZATION, 94
The Use and Handling of Cosmid Clones and Libraries, 98
PREPARATION OF A COSMID LIBRARY, 99
PROTOCOLS
Preparation of In Vitro Packaging Extracts, 105
Preparation and Verification of Packaging Strains, 105
Preparation of Sonicated Extract from Induced BHB2690 (Prehead Lysate
[PL]), 106
Preparation of Frozen Thawed Lysate of Induced BHB2688 Cells
(Packaging Protein Lysate [PPL], 108
Preparation of Dual cos Site Vector (sCos 1) DNA, 110
Purification of Dual cos Site (sCos 1) Vector DNA, 110
Digestion of Cloning Arms with Xbal and BamHI, 111
Preparation of Single cos Site Vector (pWE15) DNA, 114
Purification of Single cos Site Vector (pWE15) DNA, 114
Preparative Digestion of pWE15 Vector with BamHI, 115
Preparation of Genomic Insert DNA for Cloning into Dual cos Site Vectors, 117
Partial Digest of Genomic DNA with Mbol, 119
Test Ligation for Vector pWE 15, 121
Preparation of Insert DNA for Cloning into Single cos Site Vectors, 122
Large volume Method for DNA Isolation from Chromosomes, 124
Stacked pellet Method for DNA Isolation from Chromosomes, 126
Library Preparation from Flow cytometry sorted Chromosomes, 128
Ligation, In Vitro Packaging, and Plating, 130
Subcloning YACs into Cosmids, 133
Purification of YAC DNA, 133
Preparation and Ligation of YAC DNA with Mbol when DNA Is Limiting
and Irreplaceable, 134
Preparation and Ligation of YAC DNA with Mbol, 135
HANDLING OF COSMID LIBRARIES, 137
PROTOCOLS
Preparation of Archive Copies of Libraries, 142
Amplification of Cosmid Libraries, 144
Preparation of Arrayed Cosmid Libraries, 145
Recovering Clones, 147
Replication of Arrayed Cosmid Libraries, 148
Preparation of Filter Arrays (Grids) from Arrayed Clone Libraries, 150
Transfer of Colonies to Filters, 150
Posttransfer Treatment of Filters, 151
High density (Automated) Cosmid Arrays, 153
GENERAL CHARACTERIZATION OF COSMID LIBRARIES, 155
HYBRIDIZATION OF PROBES TO ARRAYED COSMID FILTERS, 156
PROTOCOLS
Simple Screening of Cosmid Libraries by Filter Hybridization, 158
Plating the Library, 158
Making a Replica transfer of the Colonies on Agar, 158
Screening the Library, 159
Viii Contents
Hybridization of YAC Probes to Cosmid Arrays, 162
Preparation of a YAC Clone as a Probe, 162
Hybridization Using a Labeled Probe, 163
Formamide free Hybridization to Arrayed Filters, 165
PERT Suppression Hybridization, 167
ANALYSIS OF SELECTED CLONES, 168
PROTOCOLS
Manual Modified Boiling Method for the Preparation of Cosmid DNA, 174
Manual Alkaline Lysis Preparation of Cosmid DNA, 176
Automated DNA Preparation and Restriction Enzyme Digestion Using the Biomek 1000, 178
Partial Digestion Mapping Using T3 and T7 RNA Polymerase Sites in sCOS and pWE
Vectors, 180
Notl Digest, 180
Partial Digest of Mrtl digested Cosmid DNA, 181
Preparation and Hybridization of Radioactive Probes Complementary to
the Cosmid Ends, 182
cos Site Mapping Using X Terminase, 184
Hybridization with cos Site Arm Probes, 185
Preparation of End specific RNA Probes, 186
CONSTRUCTION OF COSMID CLONE CONTIGS BY FINGERPRINTING, 188
PROTOCOLS
Restriction enzyme based Fingerprinting, 193
Digestion of Cosmid DNA and Separation of Digested Products, 193
Transfer of DNA to Membrane, 194
Labeling and Hybridization of Probe, 195
End labeling Restriction Fingerprinting, 197
3 Cloning into Bacteriophage PI Vectors, 203
N. Sternberg
GENERAL PRINCIPLES OF THE BACTERIOPHAGE PI LIFE CYCLE, 204
GENERAL PRINCIPLES OF BACTERIOPHAGE PI PACKAGING, 204
BACTERIOPHAGE PI CLONING VECTORS, 205
GENERAL CLONING STRATEGY, 206
PREPARATION OF INSERT AND VECTOR DNA, 209
PROTOCOLS
Purification ofGenomic DNA, 210
Partial Digestion and Size Fractionation of Purified Genomic
DNA, 212
Purification ofpAdWsacBII Vector DNA, 215
Preparation ofpAdlOsacBII Vector Arms, 219
Ligation of Vector Arms and Insert DNA, 221
IN VITRO PACKAGING, 222
PROTOCOLS
Preparation ofPacase (Stage I) Extract for Packaging Bacteriophage PI, 223
Preparation of Head Tail (Stage II) Extract for Packaging Bacteriophage PI, 225
Contents JX
In Vitro Packaging ofLigated Vector I Insert DNA, 228
GENERATING BACTERIOPHAGE PI COLONIES BY INFECTING HOST CELLS
WITH PACKAGED DNA, 231
PROTOCOL
Generation of Bacteriophage PI Clones, 232
ISOLATION AND ANALYSIS OF BACTERIOPHAGE PI DNA, 234
PROTOCOL
Isolation of Bacteriophage PI Plasmid DNA by Alkaline Lysis, 235
4 Bacterial Artificial Chromosomes, 241
B. Birren, V. Mancino, H. Shizuya
VECTORS FOR CLONING LARGE DNA FRAGMENTS, 242
PROCEDURES IN BAC CLONING, 244
PREPARATION OF BAC VECTOR DNA, 246
PROTOCOLS
Purification of BAC Vector DNA, 249
Restriction Digestion of Vector DNA and Treatment with
Phosphatase, 253
Testing the Quality of the Linearized Vector DNA, 255
Testing the Quality of the Dephosphorylated Vector DNA, 257
PREPARATION OF INSERT DNA, 258
PROTOCOLS
Preparation, Digestion, and Size Fractionation of Insert DNA, 261
Running a Second Preparative Sizing Gel, 266
RECOVERY AND HANDLING OF LARGE DNA, 267
LIGATION, 268
PROTOCOLS
Purifying Size selected DNA for Ligation, 269
Ligating Size selected DNA to Vector, 271
TRANSFORMATION OF CELLS WITH LARGE DNA BY
ELECTROPORATION, 274
PROTOCOLS
Preparation of Cells Competent for Electrop oration, 276
Electroporation ofLigated BAC Vector I Insert DNA, 278
DETERMINING THE EFFICIENCY OF LIGATION, 280
OPTIMIZING CLONING EFFICIENCY AND INSERT SIZE, 280
MANIPULATION AND ANALYSIS OF BACs, 282
PROTOCOLS
Isolation of BAC DNA by Alkaline Lysis, 286
Direct PFGE of Uncut BAC DNA for Size Estimation, 288
Excising BAC Inserts with Not/, 289
FINGERPRINTING BACs, 290
SEQUENCING OF BAC DNA, 292
SHOTGUN SEQUENCING OF BACs, 292
MODIFICATION OF BACs, 293
X Contents
5 Yeast Artificial Chromosomes, 297
E.D. Green, P. Hieter, F.A. Spencer
OVERVIEW OF YAC CLONING, 298
PRIMER ON YEAST GENETICS, 299
Yeast Life Cycle, 299
Yeast Genome, 300
Yeast Genetic Nomenclature, 301
Yeast Growth and Propagation, 302
Properties and Lysis of the Yeast Cell Wall, 303
Yeast Genetic Markers, 304
Standard Yeast Vectors, 304
Principles of Yeast Transformation, 305
Homologous Recombination in Yeast, 306
Background on Artificial Chromosomes in Yeast, 308
Additional Reference Materials on Yeast Genetics, 310
FUNDAMENTALS OF YAC CLONING, 311
Properties of YAC Vectors, 311
Properties of YAC Hosts, 312
Issues of Yeast Colony Color Relevant to YACs, 313
YAC LIBRARY CONSTRUCTION, 314
General Technical Considerations, 314
Preparation of YAC Vector Arms, 316
PURIFICATION AND SUBSEQUENT TREATMENT OF YAC VECTOR DNA, 319
PROTOCOL
Enzymatic Treatment of Purified pYAC4 DNA, 320
PREPARATION OF HMW SOURCE DNA, 322
Source DNA for YAC Construction, 322
Technical Considerations for Purifying HMW Source DNA, 322
RESTRICTION DIGESTION OF PURIFIED HMW SOURCE DNA, 323
SIZE FRACTIONATION OF DIGESTED SOURCE DNA, 325
PROTOCOL
Size Fractionation of Digested Source DNA by Preparative PFGE, 326
LIGATION OF YAC VECTOR ARMS AND HMW SOURCE DNA, 328
PROTOCOLS
Legation of YAC Vector Arms and Agarose embedded HMW Source DNA, 329
Ligation of YAC Vector Arms and HMW Source DNA in Solution, 332
FINAL PROCESSING OF LIGATED VECTOR SOURCE DNA, 333
PROTOCOL
Agarase Treatment of Agarose embedded, Ligated Vector Source DNA, 334
SPHEROPLAST TRANSFORMATION OF YAC LIGATION PRODUCTS, 336
PROTOCOL
Spheroplast Transformation, 340
ANALYSIS AND PROCESSING OF PRIMARY YAC TRANSFORMANTS, 346
ARRAYING AND LONG TERM STORAGE OF YAC LIBRARIES, 348
PROTOCOL
Arraying YAC Clones for Long term Storage, 350
Contents xi
Method 1, 350
Method 2, 351
RECOMBINATIONAL CLONING OF LARGE INSERTS IN YACs, 353
SCREENING YAC LIBRARIES, 355
Logistical Factors, 355
Quantitative Considerations, 355
Other Problems Associated with Hybridization Methods, 355
PCR BASED SCREENING OF YAC LIBRARIES, 357
Schemes for Constructing Pools of YAC Clones, 359
PURIFICATION OF DNA FROM POOLS OF YAC CLONES, 362
PROTOCOL
Preparation of Crude Yeast Cell Lysates, 365
PCR ANALYSIS OF YAC POOL DNA, 366
CONFIRMATION OF POSITIVE YACs, 367
HYBRIDIZATION BASED SCREENING OF YAC LIBRARIES, 368
Hybridization Analysis of YAC containing Yeast Colonies, 368
Arraying and Growth of YAC Clones on Membranes, 368
LYSIS OF YEAST COLONIES ON MEMBRANES, 371
PROTOCOL
Lysing Yeast Colonies on Membrane Filters, 372
SCREENING YAC LIBRARIES BY HYBRIDIZATION OF REPETITIVE ELEMENT
PCR PRODUCTS, 375
CARE AND HANDLING OF ISOLATED YAC CLONES, 376
Storage of Isolated YAC Clones, 376
Shipping YAC Clones, 376
OVERVIEW OF ANALYZING ISOLATED YACs, 378
Obtaining a Colony pure YAC Isolate, 378
Initial Growth and Characterization of an Isolated YAC Clone, 378
PURIFICATION OF GENOMIC DNA FROM YEAST, 381
PROTOCOLS
Purification of Yeast DNA in Solution by a Standard Method, 384
Purification of Yeast DNA in Solution by a Rapid Method, 388
Purification of Agarose etnbedded HMW Yeast DNA Using LIDS, 391
Purification of Concentrated Agarose etnbedded HMW Yeast DNA, 396
Purification of Agarose etnbedded HMW Yeast DNA Using Proteinase K, 399
Purification of Agarose etnbedded HMW Yeast DNA in Microcentrifuge Tubes, 402
Purification of Agarose etnbedded HMW Yeast DNA in 96 well Arrays, 404
ANALYSIS OF YACs BY PFGE, 408
PROTOCOL
General Steps for Routine PFGE based Analysis of YACs, 409
INTERPRETATION OF PFGE RESULTS, 412
YAC Size, 412
Number of YACs, 413
YAC Stability, 413
ESTABLISHING LONG RANGE RESTRICTION MAPS OF YACs, 415
COMPLETE DIGESTIONS AND MAPPING OF YACs USING INFREQUENTLY
CUTTING RESTRICTION ENZYMES, 416
¦
ii Contents
PROTOCOL
Complete Digestion ofHMW Yeast DNA with a Rare cutting Restriction Enzyme and PFGE
Analysis, 417
PARTIAL DIGESTION AND INDIRECT END LABEL MAPPING OF YACs, 422
PROTOCOL
Partial Digestion ofHMW Yeast DNA with a Rare cutting Restriction Enzyme and PFGE
Analysis, 423
ISOLATION OF YAC DNA BY PFGE, 429
PROTOCOL
Preparative Isolation of YAC DNA by PFGE, 433
USE OF YEAST WINDOW STRAINS TO FACILITATE YAC ISOLATION, 435
Transfer of YACs into Yeast Window Strains by Karl Mating, 435
STRATEGIES FOR YAC CONTIG CONSTRUCTION, 438
STS Content Mapping, 438
Hybridization based Fingerprint Analysis, 439
PCR based Fingerprint Analysis, 439
Long range Restriction Mapping by PFGE, 440
PCR BASED ANALYSIS OF YACs, 441
PROTOCOL
Direct PCR Analysis of YACs using Yeast Cells (Colony PCR), 443
ISOLATION OF YAC INSERT ENDS, 444
ISOLATION OF YAC INSERT ENDS BY SUBCLONING, 445
PROTOCOL
Plasmid based Subcloning of YAC Insert Ends, 448
RECOVERY OF YAC INSERT ENDS BY PLASMID RESCUE, 452
PROTOCOL
Plasmid Rescue Recovery of YAC Insert Ends, 454
RETROFITTING YACs TO ALLOW RECOVERY OF INSERT ENDS BY PLASMID
RESCUE, 455
PROTOCOL
Recovery of YAC Insert Ends by Vector Retrofitting followed by Plasmid Rescue, 459
ISOLATION OF YAC INSERT ENDS WITH PCR BASED METHODS, 462
REPEAT VECTOR PCR, 463
PROTOCOL
Recovery of YAC Insert Ends by Repeat vector PCR, 465
RECOVERY OF YAC INSERT ENDS BY BUBBLE PCR, 467
PROTOCOL
Bubble PCR based Recovery of YAC Insert Ends, 469
ISOLATING BACTERIAL BASED SUBCLONES FROM YACs, 474
Hybridization of Existing Genomic Libraries with YAC derived Probes, 474
Subcloning YAC Inserts into Bacterial based Vectors, 475
Analysis of YAC derived Subclones, 478
ANALYZING YACs FOR THE PRESENCE OF CHIMERIC INSERTS, 479
USING SOMATIC HYBRID CELL LINES TO DETECT CHIMERIC YACs, 480
HYBRIDIZATION BASED ANALYSIS OF SOMATIC HYBRID CELL LINES TO
ASSESS CHIMERISM, 482
Contents XJM
PROTOCOL
Assessing Chimerism by Dot Blot Hybridization Analysis ofAlu PCR Products, 483
Preparation of Dot Blots Containing Alu PCR Products from Hybrid Cell Lines,
483
Preparation of Alu PCR Probes from YAC Clones, 484
Hybridization Analysis of Alu PCR Products Generated from Hybrid Cell Lines
and YACs, 485
USING FISH TO DETECT CHIMERIC YACs, 486
USING GENETIC MAPPING TO DETECT CHIMERISM, 486
PARALLEL CHARACTERIZATION OF OVERLAPPING YACs TO DETECT
CHIMERISM, 486
METHODS FOR YAC MANIPULATION, 488
TRANSFER OF YACs BETWEEN YEAST STRAINS, 489
PROTOCOLS
YAC Transfer by a Genetic Cross, 495
YAC Transfer by Retransformation, 502
YAC Transfer by Karl~ Mating, 504
INTRODUCTION OF THE karl A15 MUTATION INTO YEAST STRAINS, 507
PROTOCOL
Introduction of kail Al 5 into a ura3 Yeast Strain, 509
INTRODUCTION OF karl A15 INTO A Iys2 YEAST STRAIN, 512
YEAST TRANSFORMATION METHODS, 513
PROTOCOLS
Lithium acetate based Yeast Transformation, 514
Electroporation based Yeast Transformation, 516
RECOMBINATION BASED YAC MODIFICATION, 518
INTEGRATIVE MODIFICATION OF YACs, 519
PROTOCOL
Introducing a Mammalian Selectable Marker into a YAC Insert by Integrative
Transformation, 522
YAC FRAGMENTATION, 524
PROTOCOL
Fragmentation of YACs by Targeting Integration at Alu and LINE Sequences, 528
TWO STEP REPLACEMENT OF SEQUENCES WITHIN YAC INSERTS, 530
PROTOCOL
YAC Insert Modification by Two step Replacement, 533
CONSTRUCTING LARGER YACs BY MEIOTIC RECOMBINATION, 536
PROTOCOL
Construction of Larger YACs by YAC YAC Recombination, 538
YAC AMPLIFICATION, 542
PROTOCOL
Amplification of YACs Containing a Gal repressible Centromere and the HSV TK Gene, 544
TRANSFER OF YACs INTO OTHER CELL TYPES, 546
PROTOCOL
Introduction of YACs into Mammalian Cells by Spheroplast Fusion, 548
XJV Contents
APPENDICES
1 Common Reagents, 567
Standard Stock Solutions, 568
Molarities of Concentrated Acids and Bases, 575
Common Laboratory Solutions, 576
Electrophoresis Buffers, Dyes, and Gel loading Solutions, 579
Media, 583
Antimicrobial Agents, 588
2 Basic Procedures, 591
Quantitation of Cell Concentration, 592
Standard Methods Used for Isolating DNA, 595
Extraction of DNA Samples with Organic Chemicals, 595
Concentration of DNA Samples by Precipitation with Alcohols, 599
Quantitation of DNA, 602
Dialysis of DNA, 606
Assessing the Extent of Radiolabeling in DNA Probes by Precipitation with TCA, 608
Dilution and Storage of Oligonucleotides, 610
Storage and Shipment of Biological Samples, 611
3 Safety Cautions, 617
4 Useful Facts, 625
Conversion Factors, 626
Genome Comparisons, 627
Average Sizes of DNA Fragments Generated by Cleavage with Restriction Enzymes, 628
Single letter Abbreviations for Amino Acids, 629
Genetic Code, 630
Codon Facts, 631
Isotope Information, 632
5 Suppliers, 633
Index, 635
|
| any_adam_object | 1 |
| building | Verbundindex |
| bvnumber | BV012675939 |
| callnumber-first | Q - Science |
| callnumber-label | QH445 |
| callnumber-raw | QH445.2 |
| callnumber-search | QH445.2 |
| callnumber-sort | QH 3445.2 |
| callnumber-subject | QH - Natural History and Biology |
| ctrlnum | (OCoLC)490398624 (DE-599)BVBBV012675939 |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01564nam a2200397 cc4500</leader><controlfield tag="001">BV012675939</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">00000000000000.0</controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">990722s1999 d||| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0879695137</subfield><subfield code="9">0-87969-513-7</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)490398624</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV012675939</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-20</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH445.2</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Genome analysis</subfield><subfield code="b">a laboratory manual</subfield><subfield code="n">3</subfield><subfield code="p">Cloning systems</subfield><subfield code="c">series ed. Eric D. Green ... vol. eds. Bruce Birren ...</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Plainview, NY</subfield><subfield code="b">Cold Spring Harbor Laboratory Press</subfield><subfield code="c">1999</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XXXII, 648 S.</subfield><subfield code="b">graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Genome Analysis</subfield><subfield code="2">cabt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">ADN recombiné - Manuels de laboratoire</subfield><subfield code="2">ram</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Chromosomes - Cartes - Technique</subfield><subfield code="2">ram</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Clonage moléculaire - Manuels de laboratoire</subfield><subfield code="2">ram</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Clonage</subfield><subfield code="2">ram</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Séquence nucléotidique</subfield><subfield code="2">ram</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Molecular cloning</subfield><subfield code="x">Laboratory manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Recombinant DNA</subfield><subfield code="x">Laboratory manuals</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Green, Eric D.</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Birren, Bruce</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="w">(DE-604)BV011728062</subfield><subfield code="g">3</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008614805&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-008614805</subfield></datafield></record></collection> |
| id | DE-604.BV012675939 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:31:44Z |
| institution | BVB |
| isbn | 0879695137 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008614805 |
| oclc_num | 490398624 |
| open_access_boolean | |
| owner | DE-20 DE-11 DE-188 |
| owner_facet | DE-20 DE-11 DE-188 |
| physical | XXXII, 648 S. graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | Cold Spring Harbor Laboratory Press |
| record_format | marc |
| spelling | Genome analysis a laboratory manual 3 Cloning systems series ed. Eric D. Green ... vol. eds. Bruce Birren ... Plainview, NY Cold Spring Harbor Laboratory Press 1999 XXXII, 648 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Genome Analysis cabt ADN recombiné - Manuels de laboratoire ram Chromosomes - Cartes - Technique ram Clonage moléculaire - Manuels de laboratoire ram Clonage ram Séquence nucléotidique ram Molecular cloning Laboratory manuals Recombinant DNA Laboratory manuals Green, Eric D. Sonstige oth Birren, Bruce Sonstige oth (DE-604)BV011728062 3 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008614805&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Genome analysis a laboratory manual Genome Analysis cabt ADN recombiné - Manuels de laboratoire ram Chromosomes - Cartes - Technique ram Clonage moléculaire - Manuels de laboratoire ram Clonage ram Séquence nucléotidique ram Molecular cloning Laboratory manuals Recombinant DNA Laboratory manuals |
| title | Genome analysis a laboratory manual |
| title_auth | Genome analysis a laboratory manual |
| title_exact_search | Genome analysis a laboratory manual |
| title_full | Genome analysis a laboratory manual 3 Cloning systems series ed. Eric D. Green ... vol. eds. Bruce Birren ... |
| title_fullStr | Genome analysis a laboratory manual 3 Cloning systems series ed. Eric D. Green ... vol. eds. Bruce Birren ... |
| title_full_unstemmed | Genome analysis a laboratory manual 3 Cloning systems series ed. Eric D. Green ... vol. eds. Bruce Birren ... |
| title_short | Genome analysis |
| title_sort | genome analysis a laboratory manual cloning systems |
| title_sub | a laboratory manual |
| topic | Genome Analysis cabt ADN recombiné - Manuels de laboratoire ram Chromosomes - Cartes - Technique ram Clonage moléculaire - Manuels de laboratoire ram Clonage ram Séquence nucléotidique ram Molecular cloning Laboratory manuals Recombinant DNA Laboratory manuals |
| topic_facet | Genome Analysis ADN recombiné - Manuels de laboratoire Chromosomes - Cartes - Technique Clonage moléculaire - Manuels de laboratoire Clonage Séquence nucléotidique Molecular cloning Laboratory manuals Recombinant DNA Laboratory manuals |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008614805&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV011728062 |
| work_keys_str_mv | AT greenericd genomeanalysisalaboratorymanual3 AT birrenbruce genomeanalysisalaboratorymanual3 |