Molecular biotechnology: principles and applications of recombinant DNA
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Washington, D.C.
ASM Press
1998
|
| Ausgabe: | 2. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXIII, 683 S. graph. Darst. |
| ISBN: | 1555811361 |
Internformat
MARC
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Datensatz im Suchindex
| _version_ | 1804127336389410816 |
|---|---|
| adam_text | Contents
Preface xix
Preface to the First Edition xxi
Fundamentals of Molecular Biotechnology 1
CHAPTER 1
The Molecular Biotechnology Revolution 3
Recombinant DNA Technology 3
Emergence of Molecular Biotechnology 4
Commercialization of Molecular Biotechnology 8
Concerns and Consequences 10
Summary 11
References 12
Review Questions 12
CHAPTER 2
Molecular Biotechnology Biological Systems 13
Prokaryotic and Eukaryotic Organisms 13
Escherichia coli 14
Saccharomyces cerevisiae 16
Eukaryotic Cells in Culture 17
Summary 17
References 17
Review Questions 18
CHAPTER 3
DNA, RNA, and Protein Synthesis 19
Structure of DNA 19
DNA Replication 22
Decoding Genetic Information: RNA and Protein 23
Translation 30
Regulation of mRNA Transcription in Bacteria 35
Regulation of mRNA Transcription in Eukaryotes 39
Summary 42
References 44
Review Questions 44
CHAPTER 4
Recombinant DNA Technology 45
Restriction Endonucleases 45
Plasmid Cloning Vectors 51
Plasmid Cloning Vector pBR322 53
Transformation and Selection 54
Other Plasmid Cloning Vectors 56
Creating and Screening a Library 59
Making a Gene Library 59
Screening by DNA Hybridization 59
Screening by Immunological Assay 65
Screening by Protein Activity 65
Cloning DNA Sequences That Encode Eukaryotic Proteins 66
Vectors for Cloning Large Pieces of DNA 68
Bacteriophage A Vectors 68
Cosmids 70
Very Large Insert Bacterial Vector Systems 72
Genetic Transformation of Prokaryotes 74
Transferring DNA into E. coli 74
Electroporation 74
Conjugation 75
Summary 76
References 76
Review Questions 77
CHAPTER 5
Chemical Synthesis, Sequencing, and Amplification
of DNA 79
Chemical Synthesis of DNA 79
The Phosphoramidite Method 80
Uses of Synthesized Oligonucleotides 84
Synthesis of Genes 85
DNA Sequencing Techniques 89
Dideoxynucleotide Procedure for Sequencing DNA 89
Using Bacteriophage M13 To Obtain Single Stranded DNA 92
Primer Walking 93
PCR 96
Cloning cDNA Ends by PCR 98
Gene Synthesis by PCR 102
Summary 106
References 107
Review Questions 107
CHAPTER 6
Manipulation of Gene Expression in Prokaryotes 109
Gene Expression from Strong and Regulatable Promoters 110
Regulatable Promoters 110
Increasing Protein Production 113
Large Scale Systems 114
Expression in Other Microorganisms 115
Fusion Proteins 117
Cleavage of Fusion Proteins 118
Uses of Fusion Proteins 118
Surface Display 122
Unidirectional Tandem Gene Arrays 124
Translation Expression Vectors 127
Increasing Protein Stability 128
Overcoming Oxygen Limitation 129
Use of Protease Deficient Host Strains 129
Bacterial Hemoglobin 129
DNA Integration into the Host Chromosome 130
Increasing Secretion 134
Metabolic Load 135
Summary 138
References 140
Review Questions 143
CHAPTER 7
Recombinant Protein Production in Eukaryotic Cells 145
Saccharomyces cerevisiae Expression Systems 146
S. cerevisiae Vectors 147
Direct Expression in S. cerevisiae 147
Secretion of Heterologous Proteins by S. cerevisiae 150
Other Yeast Expression Systems 151
Expression of Hepatitis B Virus Surface Antigen 151
Expression of Bovine Lysozyme C2 153
Cultured Insect Cell Expression Systems 154
Baculovirus Expression Vector System 155
Production of Recombinant Baculovirus 156
Construction of an E. coli lnsect Cell Baculovirus Shuttle Vector 158
Selective Purification of Recombinant Protein from Insect Cells by Affinity
Tagging 160
Mammalian Cell Expression Vectors 161
Selectable Marker Systems for Mammalian Expression Vectors 162
Expression of Two Cloned Genes in One Mammalian Cell 163
Summary 166
References 168
Review Questions 169
CHAPTER 8
Directed Mutagenesis and Protein Engineering 171
Directed Mutagenesis Procedures 172
Oligonucleotide Directed Mutagenesis with M13 DNA 173
Oligonucleotide Directed Mutagenesis with Plasmid DNA 176
PCR Amplified Oligonucleotide Directed Mutagenesis 176
Random Mutagenesis with Degenerate Oligonucleotide Primers 179
Random Mutagenesis with Nucleotide Analogs 182
Protein Engineering 182
Adding Disulfide Bonds 184
Changing Asparagine to Other Amino Acids 186
Reducing the Number of Free Sulfhydryl Residues 187
Increasing Enzymatic Activity 187
Modifying Metal Cofactor Requirements 189
Modifying Enzyme Specificity 191
Increasing Enzyme Stability and Specificity 192
Summary 192
References 193
Review Questions 194
Molecular Biotechnology of
Microbial Systems 197
CHAPTER 9
Molecular Diagnostics 199
Immunological Diagnostic Procedures 200
Enzyme Linked Immunosorbent Assay 201
Monoclonal Antibodies 201
Formation and Selection of Hybrid Cells 203
Identification of Specific Antibody Producing Hybrid Cell Lines 205
DNA Diagnostic Systems 206
Hybridization Probes 206
Diagnosis of Malaria 207
Detection of Trypanosoma cruzi 207
Nonradioactive Hybridization Procedures 208
DNA Fingerprinting 212
Random Amplified Polymorphic DNA 212
Molecular Diagnosis of Genetic Disease 215
Sickle Cell Anemia 215
The PCR/OLA Procedure 216
Genotyping with Fluorescence Labeled PCR Primers 219
Mutation at Different Sites within One Gene 219
Prospects 222
Summary 222
References 223
Review Questions 224
CHAPTER 1 0
Microbial Production of Therapeutic Agents 227
Pharmaceuticals 228
Isolation of Interferon cDNAs 228
Engineering Human Interferons 230
Engineering Human Growth Hormone 231
Optimizing Gene Expression 232
Enzymes 233
DNasel 233
Alginate Lyase 233
Monoclonal Antibodies as Therapeutic Agents 235
Structure and Function of Antibodies 235
Preventing Rejection of Transplanted Organs 237
Chemically Linked Monoclonal Antibodies 237
Human Monoclonal Antibodies 238
Hybrid Human Mouse Monoclonal Antibodies 240
Production of Antibodies in E. coli 243
HIV Therapeutic Agents 247
Summary 249
References 250
Review Questions 252
CHAPTER 11
Vaccines 253
Subunit Vaccines 255
Herpes Simplex Virus 256
Foot and Mouth Disease 257
Tuberculosis 258
Peptide Vaccines 259
Genetic Immunization 260
Attenuated Vaccines 263
Cholera 263
Salmonella Species 265
Leishmania Species 265
Vector Vaccines 267
Vaccines Directed against Viruses 267
Vaccines Directed against Bacteria 272
Bacteria as Antigen Delivery Systems 272
Summary 273
References 274
Review Questions 276
CHAPTER 1 2
Synthesis of Commercial Products by Recombinant
Microorganisms 277
Restriction Endonucleases 277
Small Biological Molecules 280
Synthesis of l Ascorbic Acid 280
Microbial Synthesis of Indigo 284
Synthesis of Amino Acids 286
Antibiotics 289
Cloning Antibiotic Biosynthesis Genes 290
Synthesis of Novel Antibiotics 292
Engineering Polyketide Antibiotics 294
Improving Antibiotic Production 296
Biopolymers 299
Engineering Xanthomonas campestris for Inexpensive Xanthan Gum
Production 300
Isolation of Melanin Biosynthesis Genes 301
Synthesis of an Animal Adhesive Biopolymer in Microbial Cells 302
Microbial Synthesis of Rubber 304
Microbial Production of Polyhydroxyalkanoates 304
Summary 306
References 307
Review Questions 309
CHAPTER 1 3
Bioremediation and Biomass Utilization 311
Microbial Degradation of Xenobiotics 311
Genetic Engineering of Biodegradative Pathways 316
Manipulation by Transfer of Plasmids 318
Manipulation by Gene Alteration 318
Utilization of Starch and Sugars 324
Commercial Production of Fructose and Alcohol 324
Improving Fructose and Alcohol Production 326
Zymomonas mobilis 330
Silage Fermentation 332
Utilization of Cellulose 333
Components of Lignocellulose 334
Isolation of Prokaryotic Cellulase Genes 336
Isolation of Eukaryotic Cellulase Genes 338
Manipulation of Cellulase Genes 339
Production of Single Cell Protein 341
Summary 342
References 343
Review Questions 345
CHAPTER 1 4
Plant Growth Promoting Bacteria 347
Nitrogen Fixation 348
Nitrogenase 350
Components of Nitrogenase 350
Genetic Engineering of the Nitrogenase Gene Cluster 352
Hydrogenase 356
Hydrogen Metabolism 356
Genetic Engineering of Hydrogenase Genes 357
Nodulation 359
Competition among Nodulating Organisms 359
Genetic Engineering of Nodulation Genes 359
Biocontrol of Pathogens 364
Siderophores 364
Antibiotics 366
Enzymes 367
Ice Nucleation and Antifreeze Proteins 369
Growth Promotion by Free Living Bacteria 370
Summary 372
References 373
Review Questions 374
CHAPTER 15
Microbial Insecticides 377
Insecticidal Toxin of Bacillus thuringiensis 378
Mode of Action and Use 378
Toxin Gene Isolation 382
Engineering of B. thuringiensis Toxin Genes 383
Baculoviruses as Biocontrol Agents 391
Mode of Action 391
Genetic Engineering for Improved Biocontrol 392
Summary 394
References 395
Review Questions 397
CHAPTER 1 6
Large Scale Production of Proteins from Recombinant
Microorganisms 399
Principles of Microbial Growth 401
Batch Fermentation 401
Fed Batch Fermentation 403
Continuous Fermentation 404
Maximizing the Efficiency of the Fermentation Process 406
High Density Cell Cultures 407
Bioreactors 408
Typical Large Scale Fermentation Systems 412
Two Stage Fermentation in Tandem Airlift Reactors 412
Two Stage Fermentation in a Single Stirred Tank Reactor 414
Batch versus Fed Batch Fermentation 415
Harvesting Microbial Cells 415
Disrupting Microbial Cells 417
Downstream Processing 419
Protein Solubilization 420
Summary 420
References 421
Review Questions 423
Eukaryotic Systems 425
CHAPTER 1 7
Genetic Engineering of Plants: Methodology 427
Plant Transformation with the Ti Plasmid of Agrobacterium
tumefaciens 428
Ti Plasmid Derived Vector Systems 431
Physical Methods of Transferring Genes to Plants 434
Microprojectile Bombardment 435
Use of Reporter Genes in Transformed Plant Cells 436
Manipulation of Gene Expression in Plants 437
Isolation and Use of Different Promoters 439
Targeting Foreign DNA to the Chloroplast Genome 440
Production of Marker Free Transgenic Plants 441
Summary 442
References 443
Review Questions 444
CHAPTER 1 8
Genetic Engineering of Plants: Applications 445
Development of Pathogen and Herbicide Resistant Plants 445
Insect Resistant Plants 445
Virus Resistant Plants 453
Herbicide Resistant Plants 458
Fungus and Bacterium Resistant Plants 460
Development of Stress and Senescence Tolerant Plants 461
Oxidative Stress 462
Salt Stress 463
Fruit Ripening 463
Genetic Manipulation of Flower Pigmentation 465
Modification of Plant Nutritional Content 467
Amino Acids 467
Lipids 469
Modification of Food Plant Taste and Appearance 470
Preventing Discoloration 470
Sweetness 471
Plants as Bioreactors 472
Antibodies 472
Polymers 472
Foreign Proteins in Seeds 473
Summary 473
References 474
Review Questions 477
CHAPTER 19
Transgenic Animals 479
Transgenic Mice: Methodology 480
Retroviral Vector Method 481
DNA Microinjection Method 481
Engineered Embryonic Stem Cell Method 484
Cloning by Nuclear Transfer 488
Yeast Artificial Chromosome Transgenesis 491
Transgenic Mice: Applications 493
Transgenic Cattle 497
Transgenic Sheep, Goats, and Pigs 498
Transgenic Birds 500
Transgenic Fish 502
Summary 503
References 503
Review Questions 505
CHAPTER 20
Human Molecular Genetics 507
Genetic Linkage and Gene Mapping 509
Detection and Estimation of Genetic Linkage in Humans 512
The Logarithm of the Likelihood Ratio Method of Linkage Analysis:
Lod Score 514
Genetic Mapping of Human Chromosomes 518
Genetic Polymorphism 518
Restriction Fragment Length Polymorphism 519
Short Tandem Repeat Polymorphism 522
Mapping of a Genetic Disease Locus to a Chromosome Location 525
Multilocus Mapping of Human Chromosomes 528
Inserting a Disease Gene into a Linkage Map 529
Radiation Hybrid Mapping 529
Physical Mapping of the Human Genome 531
Assembling Contigs from YAC, BAC, and PAC Libraries 532
Assembling Contigs from Cosmid, PI, and A Libraries 534
Transcriptional Mapping 535
Cloning Human Disease Genes 537
Detection of Mutations in Human Genes 537
Functional Gene Cloning 538
Candidate Gene Cloning 539
Positional Gene Cloning 539
Positional Candidate Gene Cloning 546
The Human Genome Project 547
Summary 549
References 551
Review Questions 552
CHAPTER 2 1
Human Gene Therapy 555
Ex Vivo Gene Therapy 560
In Vivo Gene Therapy 566
Viral Gene Delivery Systems 566
Retrovirus Vector System 566
Adenovirus Vector System 567
Adeno Associated Virus Vector System 570
Herpes Simplex Virus Vector System 570
Nonviral Gene Delivery Systems 575
Prodrug Activation Therapy 577
Nucleic Acid Therapeutic Agents 579
In Vivo Antisense RNA Production 580
Antisense Oligonucleotides as Therapeutic Agents 580
Nucleic Acid Pharmaceuticals 583
Ribozymes as Therapeutic Agents 584
Oligonucleotide Correction of Genetic Conditions 585
Summary 587
References 588
Review Questions 590
Regulating and Patenting Molecular
Biotechnology 591
CHAPTER 22
Regulating the Use of Biotechnology 593
Regulating Recombinant DNA Technology 594
Regulating Food and Food Ingredients 595
Chymosin 596
Tryptophan 597
Bovine Somatotropin 598
Deliberate Release of Genetically Engineered Organisms 599
Ice Minus Pseudomonas syringae 599
Open Field Tests of Other Genetically Engineering Organisms 601
Human Gene Therapy 603
Development of a Policy for Somatic Cell Gene Therapy 603
Accumulation of Defective Genes in Future Generations 605
Human Germ Line Gene Therapy 605
Cloning Humans? 606
Summary 607
References 608
Review Questions 609
CHAPTER 23
Patenting Biotechnology Inventions 611
Patenting 612
Patenting in Different Countries 614
Patenting DNA Sequences 615
Patenting Multicellular Organisms 617
Patenting and Fundamental Research 618
Summary 619
References 620
Review Questions 620
Glossary 623
Index 663
|
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| publisher | ASM Press |
| record_format | marc |
| spelling | Glick, Bernard R. Verfasser aut Molecular biotechnology principles and applications of recombinant DNA Bernard R. Glick and Jack J. Pasternak 2. ed. Washington, D.C. ASM Press 1998 XXIII, 683 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Gentechnologie (DE-588)4071722-7 gnd rswk-swf Biotechnologie (DE-588)4069491-4 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Rekombinante DNS (DE-588)4225578-8 gnd rswk-swf (DE-588)4123623-3 Lehrbuch gnd-content Rekombinante DNS (DE-588)4225578-8 s Molekulargenetik (DE-588)4039987-4 s DE-604 Biotechnologie (DE-588)4069491-4 s Molekularbiologie (DE-588)4039983-7 s 1\p DE-604 Gentechnologie (DE-588)4071722-7 s 2\p DE-604 Pasternak, Jack J. Verfasser aut HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008614563&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Glick, Bernard R. Pasternak, Jack J. Molecular biotechnology principles and applications of recombinant DNA Gentechnologie (DE-588)4071722-7 gnd Biotechnologie (DE-588)4069491-4 gnd Molekulargenetik (DE-588)4039987-4 gnd Molekularbiologie (DE-588)4039983-7 gnd Rekombinante DNS (DE-588)4225578-8 gnd |
| subject_GND | (DE-588)4071722-7 (DE-588)4069491-4 (DE-588)4039987-4 (DE-588)4039983-7 (DE-588)4225578-8 (DE-588)4123623-3 |
| title | Molecular biotechnology principles and applications of recombinant DNA |
| title_auth | Molecular biotechnology principles and applications of recombinant DNA |
| title_exact_search | Molecular biotechnology principles and applications of recombinant DNA |
| title_full | Molecular biotechnology principles and applications of recombinant DNA Bernard R. Glick and Jack J. Pasternak |
| title_fullStr | Molecular biotechnology principles and applications of recombinant DNA Bernard R. Glick and Jack J. Pasternak |
| title_full_unstemmed | Molecular biotechnology principles and applications of recombinant DNA Bernard R. Glick and Jack J. Pasternak |
| title_short | Molecular biotechnology |
| title_sort | molecular biotechnology principles and applications of recombinant dna |
| title_sub | principles and applications of recombinant DNA |
| topic | Gentechnologie (DE-588)4071722-7 gnd Biotechnologie (DE-588)4069491-4 gnd Molekulargenetik (DE-588)4039987-4 gnd Molekularbiologie (DE-588)4039983-7 gnd Rekombinante DNS (DE-588)4225578-8 gnd |
| topic_facet | Gentechnologie Biotechnologie Molekulargenetik Molekularbiologie Rekombinante DNS Lehrbuch |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008614563&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT glickbernardr molecularbiotechnologyprinciplesandapplicationsofrecombinantdna AT pasternakjackj molecularbiotechnologyprinciplesandapplicationsofrecombinantdna |