Fluorescent and luminescent probes for biological activity: a practical guide to technology for quantitative real-time analysis
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
San Diego [u.a.]
Acad. Press
1999
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Biological techniques
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXV, 647 S. Ill., graph. Darst. |
| ISBN: | 0124478360 |
Internformat
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| 245 | 1 | 0 | |a Fluorescent and luminescent probes for biological activity |b a practical guide to technology for quantitative real-time analysis |c ed. by W. T. Mason |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a San Diego [u.a.] |b Acad. Press |c 1999 | |
| 300 | |a XXV, 647 S. |b Ill., graph. Darst. | ||
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| 650 | 7 | |a Biologische activiteit |2 gtt | |
| 650 | 7 | |a Biologische markers |2 gtt | |
| 650 | 2 | |a Colorants fluorescents | |
| 650 | 2 | |a Cytophotométrie | |
| 650 | 7 | |a Fluorescentie |2 gtt | |
| 650 | 4 | |a Luminescent probes | |
| 650 | 7 | |a Luminescentie |2 gtt | |
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| 650 | 7 | |a Spectrometrie |2 gtt | |
| 650 | 4 | |a Cytophotometry | |
| 650 | 4 | |a Fluorescent Dyes | |
| 650 | 4 | |a Fluorescent probes | |
| 650 | 4 | |a Microscopy, Fluorescence | |
| 650 | 4 | |a Molecular Biology | |
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Datensatz im Suchindex
| _version_ | 1804127297478852608 |
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| adam_text | BIOLOGICAL TECHNIQUES FLUORESCENT AND LUMINESCENT PROBES FOR BIOLOGICAL
ACTIVITY A PRACTICAL GUIDE TO TECHNOLOGY FOR QUANTITATIVE REAL-TIME
ANALYSIS SECOND EDITION EDITED BY TECHNISCHE URVVERSITAT DARMSTADT WT
MASON FACHBEREICH 10 *BIOLOGIE * B I B I I O T H E K * LIFE SCIENCE
RESOURCES LTD SCHNITTSPAHNSTRARE 10 CAMBRIDGE, UK D-64287 DARMSTA D T
IFTV.-NR. ACADEMIC PRESS SAN DIEGO * LONDON * BOSTON NEW YORK * SYDNEY *
TOKYO * TORONTO CONTENTS SERIES PREFACE V PREFACE VII CONTRIBUTORS IX
PART I INTRODUCTION TO FLUORESCENCE MICROSCOPY CHAPTER ONE FLUORESCENCE
MICROSCOPY 3 IS. PLOEM 1.1 INTRODUCTION 3 1.2 MICROSCOPE DESIGN 4 1.3
TYPES OF ILLUMINATION 5 1.4 LIGHT SOURCES 7 1.5 FILTERS 9 1.6 OBJECTIVES
AND EYEPIECES 12 REFERENCES 13 PART II OPTICAL PROBES AND THEIR
APPLICATIONS CHAPTER TWO INTRODUCTION TO FLUORESCENT PROBES: PROPERTIES,
HISTORY AND APPLICATIONS 17 F.H. KASTEN 2.1 INTRODUCTION 17 2.2 NATURE
OF FLUORESCENCE AND PROPERTIES OF FLUORESCENT PROBES 18 2.3 HISTORICAL
DEVELOPMENTS 19 2.4 APPLICATIONS OF FLUOROCHROMES IN HISTOLOGY AND
MICROBIOLOGY 22 2.5 INTRODUCTION OF ACRIDINE ORANGE INTO CELL
PHYSIOLOGY, CYTOLOGY AND CYTOCHEMISTRY 23 2.6 GENERAL APPLICATIONS OF
FLUORESCENT PROBES 37 ACKNOWLEDGEMENTS 38 REFERENCES 38 CONTENTS XIII
CHAPTER THREE INTRACELLULAR ION INDICATORS 40 R.P. HAUGLAND & I.D.
JOHNSON 3.1 INTRODUCTION . 4 0 3.2 GENERAL PROPERTIES OF INTRACELLULAR
ION INDICATORS 40 3.3 R EXAMPLES OF INTRACELLULAR ION INDICATORS 46 3.4
CONCLUSIONS 49 ACKNOWLEDGEMENTS 49 REFERENCES ^ 49 CHAPTER FOUR
FLUORESCENT IMAGING OF NUCLEIC ACIDS AND PROTEINS IN GELS 51 V.L. SINGER
& R.P. HAUGLAND 4.1 INTRODUCTION 51 4.2 GENERAL PROPERTIES OF
FLUORESCENT NUCLEIC ACID STAINS 52 4.3 EXAMPLES OF FLUORESCENT NUCLEIC
ACID GEL STAINS 52 4.4 GENERAL PROPERTIES OF FLUOROPHORE LABELS USED TO
DETECT NUCLEIC ACIDS 55 4.5 GENERAL PROPERTIES OF FLUORESCENT PROTEIN
GEL STAINS 58 4.6 EXAMPLES OF FLUORESCENT PROTEIN GEL STAINS 58 4.7
PROTEIN LABELLING 61 4.8 CONCLUSIONS 61 ACKNOWLEDGEMENTS J 61 REFERENCES
61 PART III USING OPTICAL PROBES IN CELLS - PRACTICALITIES, PROBLEMS AND
PITFALLS CHAPTER FIVE INTRODUCING AND CALIBRATING FLUORESCENT PROBES IN
CELLS AND ORGANELLES 6 5 D.N. BOWSER, S.H. CODY, P.N. DUBBIN & D.A.
WILLIAMS 5.1 INTRODUCTION 65 5.2 GENERAL PRINCIPLES OF THE LOADING
PROCESS 66 5.3 GENERAL PRINCIPLES OF THE CALIBRATION PROCESS 69 5.4
PUTTING PRINCIPLES INTO PRACTICE 70 ACKNOWLEDGEMENTS 80 REFERENCES
80 CHAPTER SIX ELECTROPORATION: A METHOD FOR INTRODUCTION OF
NON-PERMEABLE MOLECULAR PROBES 82 F. BOBANOVIC 6.1 INTRODUCTION 82 6.2
BASIC CONCEPT OF ELECTROPORATION 83 6.3 ELECTRIC FIELD GENERATION AND
MONITORING 85 6.4 POLARIZATION OF THE OUTER MEMBRANE 86 6.5 ELECTROPORE
FORMATION AND RESEALING 87 6.6 TRANSMEMBRANE TRANSPORT 88 6.7 PRACTICAL
CONSIDERATIONS OF ELECTROPORATION 89 6.8 EXPERIMENTAL EVIDENCE 91 6.9
SUMMARY 92 ACKNOWLEDGEMENTS 92 REFERENCES 93 XIV CONTENTS CHAPTER SEVEN
IMAGING REALITY: UNDERSTANDING MAPS OF PHYSIOLOGICAL CELL SIGNALS
MEASURED BY FLUORESCENCE MICROSCOPY AND DIGITAL IMAGING 94 S.L. SHORTE &
S. BOLSOVER __ 7.1 INTRODUCTION 94 7.2 GENERIC CONSIDERATIONS FOR THE
USE OF FLUORESCENT INDICATORS 95 7.3 OPTIMIZATION OF FLUORESCENT LIGHT
DETECTION AND BACKGROUND LIGHT CORRECTION 100 7.4 3-D SPATIAL MAPS OF
FLUORESCENT SIGNALS 102 ACKNOWLEDGEMENTS 106 REFERENCES 106 CHAPTER
EIGHT FLUORESCENT PROBES IN PRACTICE - POTENTIAL ARTIFACTS 108 I HOYLAND
1.1 INTRODUCTION 108 1.2 PHOTOBLEACHING 108 1.3 DYNAMIC RANGE 109 1.4
PROBE LOADING 110 1.5 ION CALIBRATION 111 1.6 CELL MOVEMENT AND FAST ION
FLUXES 111 1.7 AUTOFLUORESCENCE 112 1.8 INTERACTIONS BETWEEN MULTIPLE
PROBES 112 1.9 AVERAGING AND INTENSIFIER NOISE 113 1.10 PROBE LEAKAGE
AND EXOCYTOSIS 113 1.11 PROBE KINETICS 113 REFERENCES - 113 PART IV
OPTICAL PROBES FOR SPECIFIC MOLECULES, ORGANELLES AND CELLS CHAPTER NINE
ACRIDINE ORANGE AS A PROBE FOR CELL AND MOLECULAR BIOLOGY 1 17 A. V.
ZELENIN 9.1 INTRODUCTION 117 9.2 HISTORICAL REMARKS 117 9.3 AO AS A
FLUORESCENT DYE 118 9.4 SPECTRAL PROPERTIES OF AO IN COMPLEXES WITH
NUCLEIC ACIDS AND OTHER BIOPOLYMERS 118 9.5 AO IN THE STUDY OF NUCLEIC
ACIDS IN VITRO 119 9.6 AO IN NUCLEIC ACID CYTOCHEMISTRY 120 9.7 AO DNA
STAINING AFTER ACID PRETREATMENTS 122 9.8 AO IN THE STUDY OF DNA THERMAL
DENATURATION 122 9.9 AO IN THE STUDY OF THE CHROMATIN FUNCTIONAL STATE
123 9.10 FLUORESCENCE POLARIZATION OF AO IN STUDIES OF BIOPOLYMERS 126
9.11 AO IN CHROMOSOME BANDING 127 9.12 AO IN ACID POLYSACCHARIDE
HISTOCHEMISTRY 127 9.13 AO BINDING TO PROTEINS 128 9.14 AO BINDING TO A
LIVING CELL 128 9.15 AO IN THE STUDY OF CELL VIABILITY 131 9.16 AO IN
THE STUDY OF APOTOPSIS 131 9.17 AO IN FLOW CYTOMETRY 132 9.18 OTHER
APPLICATIONS OF AO 132 REFERENCES 132 CONTENTS XV CHAPTER TEN
FLUORESCENT LIPID ANALOGUES: APPLICATIONS IN CELL AND MEMBRANE BIOLOGY
136 J. W. KOK & D. HOEKSTRA 10.1 INTRODUCTION 136 10.2 FLUORESCENT LIPID
ANALOGUES 138 10.3 APPLICATIONS 143 ACKNOWLEDGEMENTS 154 REFERENCES X
154 CHAPTER ELEVEN OPTICAL PROBES FOR CYCLIC AMP 156 S.R. ADAMS, B.J.
BACSKAI, S.S. TAYLOR & R.Y. TSIEN 11.1 RATIONALE FOR CREATING OPTICAL
PROBES FOR CYCLIC AMP 156 11.2 PREVIOUS METHODS FOR MEASURING CAMP OR
IMAGING RELATED MOLECULES 157 11.3 ALTERNATIVE CAMP BINDING SITES 158
11.4 PROPERTIES OF A-KINASE 158 11.5 FLUORESCENT LABELLING OF A-KINASE
160 11.6 PROPERTIES OF FICRHR 163 11.7 INTRODUCTION OF FICRHRINTO CELLS
166 11.8 IMAGING OF FICRHR AND FREE CAMP 167 11.9 APPLICATIONS 170
REFERENCES 171 PART V TECHNOLOGY FOR QUALITATIVE AND QUANTITATIVE
DETECTION OF OPTICAL PROBES IN LIVING CELLS CHAPTER TWELVE QUANTITATIVE
DIGITAL IMAGING OF BIOLOGICAL ACTIVITY IN LIVING CELLS WITH
ION-SENSITIVE FLUORESCENT PROBES 175 W.T. MASON, J. DEMPSTER, J.
HOYLAND, T.J. MCCANN, B. SOMASUNDARAM & W. O BRIEN 12.1 INTRODUCTION 175
12.2 FLUORESCENT PROBES FOR LIVING CELL FUNCTION 176 12.3 OBSERVING
BIOLOGICAL ACTIVITY IN REAL TIME 178 12.4 RATIOMETRIC IMAGING OF
ION-SENSITIVE FLOURESCENT PROBES 178 12.5 IMAGING STRATEGIES 178 12.6
DIGITAL IMAGE PROCESSING 181 12.7 DATA PRESENTATION 182 12.8 CONFOCAL
LASER SCANNING MICROSCOPY - OPTICAL APPROACHES TO ENHANCED IMAGE
RESOLUTION .. 182 12.9 A NOVEL HIGH-SPEED DIGITAL CONFOCAL MICROSCOPE
183 12.10 DIGITAL DECONVOLUTION AND DIGITAL CONFOCAL MICROSCOPY - SOFT
APPROACHES TO ENHANCED IMAGE RESOLUTION 185 12.11 FLUORESCENT
MEASUREMENTS OF CYTOSOLIC IONS - COMBINED PHOTOMETRY WITH
ELECTROPHYSIOLOGY 186 12.12 PHOTOMETRIC MEASUREMENTS IN SINGLE CELLS 186
12.13 MEASUREMENT OF FLUORESCENT LIGHT - PHOTOMETRY VERSUS IMAGING 187
12.14 PHOTOMULTIPLIER TUBE TECHNOLOGY . 189 12.15 PHOTON COUNTING VERSUS
PHOTOCURRENT INTEGRATION 189 12.16 EXCITATION FILTER SWITCHING 190 12.17
DUAL-EMISSION PROBES 190 12.18 ELECTROPHYSIOLOGY COMBINED WITH
PHOTOMETRY OR IMAGING 191 12.19 DATA ACQUISITION 191 XVI CONTENTS 12.20
CONCORD - INTEGRATING IMAGING, PHOTOMETRY AND ELECTROPHYSIOLOGY ON A
SINGLE WORKSTATION FOR ION IMAGING EXPERIMENTS 191 12.21 CELL CULTURE
AND LOADING OF FLUORESCENT PROBES 193 12.22 CALIBRATION OF ION-SENSITIVE
DYES IN LIVING CELLS AND IN SOLUTION 193 12.23 DERIVING SPECTRA DATA
FROM OPTICAL PROBES IN SITU - THE SPECTRALWIZARD 194 - 12.24 SUMMARY
195 REFERENCES . * 195 CHAPTER THIRTEEN FAST PHOTOMETRIC MEASUREMENTS OF
CELL FUNCTION COMBINED WITH ELECTROPHYSIOLOGY 196 J. DEMPSTER 13.1
FLUORESCENT LIGHT MEASUREMENT 197 13.2 A
FLUORESCENCE/ELECTROPHYSIOLOGICAL RECORDING SYSTEM 197 13.3 THE
PHOTOMULTIPLIER TUBE 199 13.4 DUAL-EMISSION DYE MEASUREMENT SYSTEMS 200
13.5 DUAL-EXCITATION DYE MEASUREMENT SYSTEMS 201 13.6 ANALOGUE SIGNAL
DIGITIZATION 203 13.7 FLUORESCENCE MEASUREMENT SYSTEMS 203 13.8 SOFTWARE
FOR RECORDING FLUORESCENCE SIGNALS 205 13.9 THE CHART RECORDER
PARADIGM 205 13.10 THE OSCILLOSCOPE PARADIGM 205 13.11 LEAK CURRENT
SUBTRACTION 206 13.12 COMPUTER SYSTEM DESIGNS ^ 207 13.13 CONCLUSION
208 REFERENCES 208 EQUIPMENT SUPPLIERS 208 CHAPTER FOURTEEN
POTENTIOMETRIC MEMBRANE DYES AND IMAGING MEMBRANE POTENTIAL IN SINGLE
CELLS 210 L.M. LOEW 14.1 INTRODUCTION 210 14.2 OPTIMIZATION OF DYE
INDICATOR SENSITIVITY 211 14.3 MAPPING MEMBRANE POTENTIAL BY DIGITAL
FLUORESCENCE MICROSCOPY 215 14.4 CONCLUSION 219 ACKNOWLEDGEMENTS 219
REFERENCES 219 CHAPTER FIFTEEN FAST MULTISITE OPTICAL MEASUREMENT OF
MEMBRANE POTENTIAL, WITH TWO EXAMPLES 22 2 J.-Y. WU, L.B. COHEN & C.X.
FOLK 222 223 224 225 229 233 235 236 236 15.1 15.2 15.3 15.4 15.5 15.6
15.7 INTRODUCTION SIGNAL TYPE DYES MEASURING TECHNOLOGY TWO EXAMPLES
POPULATION SIGNALS FROM VERTEBRATE BRAIN FUTURE DIRECTIONS
ACKNOWLEDGEMENTS REFERENCES CONTENTS XVII CHAPTER SIXTEEN IMAGING
MEMBRANE POTENTIAL CHANGES IN INDIVIDUAL NEURONS 238 S. ANTIC & D.
ZECEVIC 16.1 INTRODUCTION 238 16.2 EXTRACELLULAR APPLICATION OF
VOLTAGE-SENSITIVE DYES 240 M6.3 INTRACELLULAR APPLICATION OF
VOLTAGE-SENSITIVE DYES 240 16.4 DYE INJECTION 241 16.5 OPTICAL RECORDING
241 16.6 MULTISITE RECORDING 242 16.7 COMPARISON OF OPTICAL AND
ELECTRICAL SIGNALS 243 16.8 DYE SENSITIVITY (DF/F) AND SIGNAL-TO-NOISE
RATIO 244 16.9 CALIBRATION OF THE VOLTAGE-SENSITIVE DYE MEASUREMENTS IN
TERMS OF MEMBRANE POTENTIAL 244 16.10 PHARMACOLOGICAL EFFECTS AND
PHOTODYNAMIC DAMAGE .. 245 16.11 IMAGING SPIKE TRIGGER ZONE 246 16.12
VERTEBRATE NEURONS 246 16.13 SUMMARY 247 REFERENCES 247 PART VI USING
NOVEL INDICATIONS FOR GENETIC, MOLECULAR AND CELLULAR FUNCTION CHAPTER
SEVENTEEN BIOLUMINESCENT AND CHEMILUMINESCENT INDICATORS FOR MOLECULAR
SIGNALLING AND FUNCTION IN LIVING CELLS - 251 G.B. SALA-NEWBY, J.M.
KENDALL, HE. JONES, K.M. TAYLOR, M.N. BADMINTON, D.H. LLEWELLYN & A.K.
CAMPBELL 17.1 THE NATURAL HISTORY OF BIO-AND CHEMILUMINESCENCE . 251
17.2 THE ANALYTICAL POTENTIAL OF CHEMILUMINESCENT COMPOUNDS 253 17.3
APPLICATION OF CHEMI-AND BIOLUMINESCENCE TO LIVING CELLS 256 17.4
BIOLUMINESCENT REPORTER GENES ) 261 17.5 BIOLUMINESCENT INDICATORS FOR
MOLECULAR SIGNALLING IN LIVE CELLS . 266 17.6 CONCLUSIONS AND FUTURE
PROSPECTS 269 REFERENCES 269 CHAPTER EIGHTEEN LUMINESCENCE IMAGING OF
GENE EXPRESSION IN SINGLE LIVING CELLS 273 G.A. RUTTER, A.A. CULBERT &
J.M. TAVARE 18.1 INTRODUCTION 273 18.2 GENERAL CONSIDERATIONS 274 18.3
EQUIPMENT REQUIRED 276 18.4 EXPERIMENTAL PROCEDURES 279 18.5 CONCLUDING
REMARKS . 282 ACKNOWLEDGEMENTS 282 REFERENCES 282 CHAPTER NINETEEN
ENHANCED VARIANTS OF THE GREEN FLUORESCENT PROTEIN FOR GREATER
SENSITIVITY, DIFFERENT COLOURS AND DETECTION OF APOPTOSIS 284 S.R. KAIN
19.1 INTRODUCTION 284 19.2 GFP VARIANTS 286 19.3 DETECTION OF APOPTOSIS
WITH GFP 289 REFERENCES 291 XVIII CONTENTS CHAPTER TWENTY RAPID
DETECTION OF MICROORGANISMS 293 R. HAGGART, N. BODSWORTH & N. FOOTE 20.1
MATERIALS AND METHODS 296 20.2 RESULTS 297 20.3 DISCUSSION 298
REFERENCES 300 PART VII APPLICATIONS OF CONFOCAL MICROSCOPY FOR OPTICAL
PROBE IMAGING CHAPTER TWENTY-ONE CONFOCAL MICROSCOPY - PRINCIPLES,
PRACTICE AND OPTIONS 303 C.J.R. SHEPPARD 21.1 INTRODUCTION 303 21.2
ADVANTAGES OF SCANNING 303 21.3 CONFOCAL MICROSCOPY 304 21.4 PRACTICAL
ASPECTS 306 21.5 SYSTEM PERFORMANCE 308 REFERENCES 309 CHAPTER
TWENTY-TWO DUAL-EXCITATION CONFOCAL FLUORESCENCE MICROSCOPY 310 K.-P YIP
& I. KURTZ 22.1 INTRODUCTION 310 22.2 MEASUREMENT OF PHJ WITH BCECF ;-*
310 22.3 DESIGN OF A DUAL-EXCITATION LASER SCANNING CONFOCAL
FLUORESCENCE MICROSCOPE FOR MEASURING PHJ WITH BCECF 312 22.4
MEASUREMENT OF PHJ IN THE CORTICAL COLLECTING TUBULE 313 22.5
MEASUREMENT OF INTRACELLULAR CA 2+ IN THE PERFUSED AFFERENT ARTERIOLE
314 22.6 FUTURE DEVELOPMENT 315 ACKNOWLEDGEMENTS 315 REFERENCES 315
CHAPTER TWENTY-THREE HIGH-SPEED CONFOCAL IMAGING IN FOUR DIMENSIONS Y.S.
PRAKASH, M.S. KANNAN & G.C. SIECK 23.1 INTRODUCTION 23.2 KEY QUESTIONS
RELATING TO DATA SAMPLING 23.3 NORAN ODYSSEY XL REAL-TIME CONFOCAL
SYSTEM 23.4 SPATIAL AND TEMPORAL RESOLUTIONS IN 2-D IMAGING 23.5 CASE
STUDIES ON 2-D RESOLUTIONS 23.6 SPATIAL AND TEMPORAL RESOLUTIONS IN 3-D
IMAGING 23.7 4-D TEMPORAL RESOLUTION 23.8 3-D AND 4-D IMAGE PROCESSING,
DISPLAY AND STORAGE 23.9 CASE STUDIES ON 3-D AND 4-D RESOLUTIONS 23.10
FUTURE DIRECTIONS ACKNOWLEDGEMENTS REFERENCES CONTENTS XIX CHAPTER
TWENTY-FOUR MULTIPHOTON FLUORESCENCE MICROSCOPY 331 G. COX & C. SHEPPARD
24.1 INTRODUCTION * 331 24.2 PRINCIPLES OF TWO-PHOTON FLUORESCENCE 331
24.3 THE COSTS AND THE BENEFITS 333 24.4 HARDWARE FOR TWO-PHOTON
MICROSCOPY 334 ACKNOWLEDGEMENTS - 335 REFERENCES 335 CHAPTER
TWENTY-FIVE HIGH-RESOLUTION CONFOCAL IMAGING OF ELEMENTARY CA 2+ SIGNALS
IN LIVING CELLS 337 P. LIPP & M.D. BOOTMAN 25.1 CALCIUM AS AN
INTRACELLULAR MESSENGER 337 25.2 INVESTIGATING ELEMENTARY CA 2+ SIGNALS
339 25.3 THE TYPE OF CONFOCAL MICROSCOPE AND THE MODE OF SCANNING 341
25.4 ADDITIONAL CONSIDERATIONS 342 25.5 SUMMARY 343 REFERENCES 343
CHAPTER TWENTY-SIX CONFOCAL FLUORESCENT MICROSCOPY USING A NIPKOW
SCANNER 344 A. ICHIHARA, T. TANAAMI, H. ISHIDA & M. SHIMIZU 26.1
INTRODUCTION 344 26.2 CONSTRUCTION OF THE CSU10 345 26.3
CHARACTERISTICS OF THE CSU10 345 26.4 SYSTEM INTEGRATION OF THE CSU10
* . * . 347 26.5 APPLICATIONS . .. 347 26.6 FUTURE PROSPECTS . 348 26.7
CONCLUSION 349 REFERENCES 349 CHAPTER TWENTY-SEVEN OPTIMIZING CONFOCAL
MICROSCOPY FOR THICK BIOLOGICAL SPECIMENS 350 S.H. CODY & D.A. WILLIAMS
21A INTRODUCTION 350 27.2 ADVANTAGES OF CONFOCAL OVER WIDE-FIELD
MICROSCOPY, ESPECIALLY FOR THICK BIOLOGICAL SPECIMENS 351 27.3
PATHLENGTH ERRORS 352 27.4 DYE CONCENTRATION AND PHOTOBLEACHING
ARTIFACTS 353 27.5 RATIOMETRIC ANALYSIS 353 27.6 ABERRATIONS AND
CONFOCAL MICROSCOPY . 354 27.7 SPHERICAL ABERRATION - 354 27.8
CHROMATIC ABERRATION , 355 27.9 AVOIDING REFRACTIVE INDEX MISMATCH . 356
ACKNOWLEDGEMENTS . 360 REFERENCES 360 XX CONTENTS CHAPTER TWENTY-EIGHT
REDOX CONFOCAL IMAGING: INTRINSIC FLUORESCENT PROBES OF CELLULAR
METABOLISM 361 B.R. MASTERS & B. CHANCE 28.1 INTRODUCTION 361 28.2
HISTORY OF THE USE OF INTRINSIC PROBES TO MONITOR CELLULAR METABOLISM
363 28.3 THE BIOCHEMICAL BASIS OF INTRINSIC FLUORESCENT PROBES IN LIVING
CELLS 364 28.4 INSTRUMENTATION FOR THE USE OF LOW-LIGHT-LEVEL
FLUORESCENT IMAGING OF LIVING CELLS AND TISSUES 365 28.5 APPLICATIONS OF
INTRINSIC FLUORESCENT REDOX PROBES TO CELLULAR METABOLISM 370 28.6
COMPARISON WITH OTHER NON-INVASIVE TECHNIQUES 372 28.7 SUMMARY AND
CONCLUSIONS 373 ACKNOWLEDGEMENTS 373 REFERENCES 373 PART VIII ADVANCED
IMAGING AND LIGHT DETECTION APPROACHES FOR OPTICAL PROBE APPLICATIONS
CHAPTER TWENTY-NINE CONFOCAL RAMAN MICROSPECTROMETRY 377 G.J. PUPPELS
29.1 INTRODUCTION 377 29.2 RAMAN SPECTROSCOPY 378 29.3 THE CONFOCAL
RAMAN MICROSPECTROMETER (CRM) 382 29.4 APPLICATIONS 385 29.5 FUTURE
DEVELOPMENTS 402 ACKNOWLEDGEMENTS 404 REFERENCES 405 CHAPTER THIRTY
SPECTRAL IMAGING OF AUTOFLUORESCENCE MOLECULES AND DNA PROBES . 407 C.
ROTHMANN, I. BAR-AM, Z. DUBINSKY, S. KATZ & Z. MALIK 30.1 INTRODUCTION
407 30.2 THE SPECTRACUBE* SYSTEM AND DESIGN 408 30.3 THE ANALYSIS OF A
SPECTRAL IMAGE 409 30.4 APPLICATIONS OF SPECTRAL IMAGING IN BIOLOGY 410
REFERENCES 412 CHAPTER THIRTY-ONE MULTIPHOTON EXCITATION MICROSCOPY AND
SPECTROSCOPY OF CELLS, TISSUES AND HUMAN SKIN IN VIVO 414 B.R. MASTERS,
PTC. SO & E. GRATTON 31.1 INTRODUCTION 414 31.2 HISTORY OF TWO-PHOTON
EXCITATION MICROSCOPY 415 31.3 PHYSICS OF MULTIPHOTON EXCITATION
PROCESSES 416 31.4 COMPARISON WITH CONFOCAL MICROSCOPY 418 31.5
INSTRUMENTATION FOR MULTIPHOTON EXCITATION MICROSCOPY, SPECTROSCOPY AND
LIFETIME MEASUREMENT 420 31.6 APPLICATIONS TO CELLS AND TISSUES 423 31.7
APPLICATION TO IN VIVO FUNCTIONAL IMAGING OF HUMAN SKIN: AN EXAMPLE OF A
THICK, HIGHLY SCATTERING IN VIVO TISSUE 425 31.8 MITIGATION OF
PHOTODAMAGE WITH MULTIPHOTON EXCITATION MICROSCOPY 428 31.9 DISCUSSION
429 CONTENTS XXI 31.10 SUMMARY AND CONCLUSION S 429 ACKNOWLEDGEMENTS
430 REFERENCES 430 CHAPTER THIRTY-TWO RAMAN SPECTROSCOPIC METHODS FOR IN
VITRO AND IN VIVO TISSUE CHARACTERIZATION 433 R. WOLTHUIS, T.C. BAKKER
SCHUT, P.J. CASPERS, H.P.J. BUSCHMAN, T.J. ROMER, H.A. BRUINING & G.J.
PUPPELS 32.1 INTRODUCTION 433 32.2 INSTRUMENTATION 434 32.3 CALIBRATION
OF RAMAN SPECTRA 437 32.4 DATA ANALYSIS _^ 440 32.5 EXAMPLES OF TISSUE
CHARACTERIZATION BY RAMAN SPECTROSCOPY 443 32.6 CONCLUSION 453
ACKNOWLEDGEMENTS 453 REFERENCES 454 CHAPTER THIRTY-THREE IN VIVO
SEMIQUANTITATIVE NADH-FLUORESCENCE IMAGING 456 G.J. PUPPELS, J.M.C.C.
COREMANS & H.A. BRUINING 33.1 INTRODUCTION 456 33.2 ORIGIN OF UV-EXCITED
(365 NM) TISSULAR FLUORESCENCE 457 33.3 METHODOLOGY OF SEMIQUANTITATIVE
IN VIVO NADH-FLUORESCENCE IMAGING - 458 33.4 INSTRUMENTATION - * - 460
33.5 EXPERIMENTAL VERIFICATION OF THE LINEAR RELATIONSHIP BETWEEN [NADH]
AND ^NADH^DIFF, 365 461 33.6 BIOMEDICAL AND CLINICAL APPLICATIONS OF
NADH-FLUORESCENCE IMAGING 461 33.7 OUTLOOK 465 ACKNOWLEDGEMENTS 465
REFERENCES 465 CHAPTER THIRTY-FOUR FLUORESCENCE LIFETIME IMAGING
MICROSCOPY (FLIM): INSTRUMENTATION AND APPLICATIONS 467 T W.J GADELLA JR
34.1 INTRODUCTION 467 34.2 EXPERIMENTAL REALIZATIONS OF
LIFETIME-RESOLVED IMAGING MICROSCOPY 468 34.3 IMPLEMENTATION OF A
WIDE-FIELD FREQUENCY-DOMAIN FLIM SYSTEM 473 34.4 EXAMPLE OF FLIM
MEASUREMENT: GFP IN LIVING CELLS 475 34.5 APPLICATIONS OF FLIM 475 34.6
CONCLUDING REMARKS 477 ACKNOWLEDGEMENTS 478 REFERENCES 478 CHAPTER
THIRTY-FJVE DETECTION OF FLUORESCENTLY LABELLED PROTEINS FOLLOWING GEL
ELECTROPHORESIS: A KEY ENABLING TECHNOLOGY FOR PROTEOMICS 480 G. SKEWS &
T. MCCANN 35.1 INTRODUCTION 480 35.2 GENERAL APPLICATION AREAS FOR
FLUORESCENT IMAGING IN ELECTROPHORESIS 480 35.3 WHAT IS ELECTROPHORESIS?
481 XXII CONTENTS 35.4 THE USE OF SLAB GELS 482 35.5 VISUALIZATION OF
PROTEINS 482 35.6 FLUORESCENT PRELABELLING OF PROTEINS 482 35.7 IMAGING
FLUORESCENT PROTEINS IN GELS 484 35.8 ALTERNATIVE STAINING AND
VISUALIZATION TECHNIQUES 485 35.9 IMAGING AREA 485 35.10 HIGH-THROUGHPUT
ACQUISITION 486 35.11 EXAMPLES OF SPECIFIC APPLICATION AREAS 486 35.12
SUMMARY 487 REFERENCES 488 PART IX CCD CAMERAS: KEY ENABLING
TECHNOLOGIES FOR OPTICAL PROBE IMAGING CHAPTER THIRTY-SIX PROPERTIES OF
LOW-LIGHT-LEVEL INTENSIFIED CAMERAS 491 P. TOMKINS & A. LYONS 36.1
INTRODUCTION 36.2 IMAGE INTENSIFIER: FIRST AND SECOND GENERATION 36.3
MAXIMIZING SIGNAL AND MINIMIZING NOISE 36.4 THERMAL NOISE 36.5
NON-THERMAL NOISE 36.6 TEMPORAL CHARACTERISTICS OF PHOTOCATHODE NOISE
36.7 GAIN USE AND ABUSE 36.8 SPECTRAL RESPONSE . ^ 36.9 COMPARISON
BETWEEN SECOND- AND THIRD-GENERATION INTENSIFIER TUBES 36.10
HIGH-RESOLUTION INTENSIFIERS 36.11 FIBREOPTICS OR LENSES FOR IMAGE
TRANSFER? 36.12 THE CHARGE-COUPLED DEVICE 36.13 FRAME AND LINE TRANSFER
CCDS 36.14 FULL-FRAME CCDS 36.15 CCD PIXEL SIZE AND DYNAMIC RANGE 36.16
READ-OUT FEATURES 36.17 HIGH-RESOLUTION DIGITAL ICCDS 36.18 FAST
READ-OUT ICCDS 36.19 DIGITAL ICCDS WITH INCREASING BIT RANGE 36.20
ANALOGUE VIDEO ICCDS 36.21 PHOTON COUNTING IMAGING 36.22 SYSTEMS
INTEGRATION 36.23 FLAT-FIELD NORMALIZATION - THE MAGIC TOUCH CHAPTER
THIRTY-SEVEN PROPERTIES OF LOW-LIGHT-LEVEL SLOW-SCAN DETECTORS R. AIKENS
37.1 INTRODUCTION 37.2 CONTEMPORARY IMAGE-ACQUISITION TECHNOLOGY 37.3
HOW CCDS WORK 37.4 THE HIGH-PERFORMANCE SLOW-SCAN CCD CAMERA 37.5
SLOW-SCAN CCD CAMERA PERFORMANCE 37.6 APPLICATIONS OF SLOW-SCAN CCD
CAMERAS 37.7 SUMMARY REFERENCES CONTENTS XXIII CHAPTER THIRTY-EIGHT
HIGH-SPEED DIGITAL CCD CAMERAS - PRINCIPLES AND APPLICATIONS 517 CD.
MACKAY 38.1 INTRODUCTION 517 38.2 THE NEED FOR HIGH-SPEED DIGITAL CCD
CAMERAS 517 38.3 OTHER TECHNOLOGICAL ADVANCES 519 38.4 SYSTEMS SELECTION
522 38.5 CONCLUSIONS 524 PART X FLOW CYTOMETRIC METHODOLOGIES FOR
MEASUREMENT OF OPTICAL PROBES IN LIVE CELLS CHAPTER THIRTY-NINE FLOW
CYTOMETRY: USE OF MULTIPARAMETER KINETICS TO EVALUATE SEVERAL ACTIVATION
PARAMETERS SIMULTANEOUSLY IN INDIVIDUAL LIVING CELLS 527 E.R. SIMONS
39.1 INTRODUCTION 527 39.2 FLUORESCENT TECHNIQUES - GENERAL ADVANTAGES
AND DISADVANTAGES 528 39.3 FLOW CYTOMETRY FOR KINETIC STUDIES OF
CELLULAR FUNCTIONS 529 39.4 TIME OF ONSET OF INITIAL RESPONSE 529 39.5
PARAMETERS WHICH CAN BE MEASURED 530 39.6 CLASSES OF FLUORESCENT PROBES
C 530 39.7 SPECIFIC PROBES FOR PARAMETERS OF CELL FUNCTION 532 39.8
NON-KINETIC APPLICATIONS OF RELEVANCE TO MULTIPARAMETER FLOW KINETICS
CORRELATIONS 537 39.9 CONCLUSION 538 ACKNOWLEDGEMENTS - 538 REFERENCES
538 CHAPTER FORTY PHOTOLABILE CAGED COMPOUNDS 540 A.M. GURNEY 40.1
INTRODUCTION 540 40.2 PROPERTIES OF A PHOTOLABILE PROBE 541 40.3 THE
CHEMISTRY OF PHOTOLABILE COMPOUNDS . 542 40.4 APPLICATION OF PHOTOLABILE
PROBES TO STUDYING BIOLOGICAL PATHWAYS 546 40.5 POTENTIAL PROBLEMS
ASSOCIATED WITH THE USE OF CAGED COMPOUNDS 548 40.6 CONCLUSION 552
ACKNOWLEDGEMENTS 552 REFERENCES 552 CHAPTER FORTY-ONE FLUORESCENT
ANALOGUES: OPTICAL BIOSENSORS OF THE CHEMICAL AND MOLECULAR DYNAMICS OF
MACROMOLECULES IN LIVING CELLS 554 K. HAHN, J. KOLEGA, J. MONTIBELLER,
R. DEBIASIO, P. POST, J. MYERS & D.L. TAYLOR 41.1 INTRODUCTION 554 41.2
MEROCAM 1 AND 2: FLUORESCENT INDICATORS OF CALCIUM-CALMODULIN BINDING
555 41.3 FLUORESCENT ANALOGUE OF MYOSIN II 557 41.4 PROTEIN-BASED
OPTICAL BIOSENSORS OF MYOSIN II REGULATORY LIGHT CHAIN PHOSPHORYLATION
562 41.5 FUTURE STUDIES 564 REFERENCES 565 XXIV CONTENTS PART XI
APPLICATIONS OF OPTICAL PROBE IMAGING TO BIOLOGICAL PROBLEMS CHAPTER
FORTY-TWO FLUORESCENCE AND LUMINESCENCE TECHNIQUES TO PROBE ION
ACTIVITIES IN LIVING PLANT CELLS - M.D. FRICKER, C. PLIETH, H. KNIGHT,
E. BLANCAFLOR, M.R. KNIGHT, N.S. WHITE & S. GILROY 42.1 INTRODUCTION
42.2 TISSUE PREPARATION, MOUNTING AND PERFUSION 42.3 SECURING THE
SPECIMEN FOR MICROSCOPY ~ 42.4 SELECTION AND USE OF FLUORESCENT PROBES
42.5 OBSERVATION AND MEASUREMENT OF DYE FLUORESCENCE 42.6 CALIBRATION IN
VITRO AND IN SITU 42.7 ADDITIONAL MEASUREMENT TECHNIQUES 42.8 USING
RECOMBINANT AEQUORIN FOR MEASUREMENT OF INTRACELLULAR CALCIUM IN PLANTS
42.9 MANIPULATION OF INTRACELLULAR EVENTS USING CAGED PROBES 42.10
PROBES FOR OTHER COMPARTMENTS 42.11 FUTURE DEVELOPMENTS ACKNOWLEDGEMENTS
REFERENCES CHAPTER FORTY-THREE NUCLEAR CALCIUM: CONCEPTS AND
CONTROVERSIES G. SHANKAR & M.A. HORTON 43.1 INTRODUCTION 43.2 THE
NUCLEAR ENVELOPE AND NUCLEAR TRANSPORTERS 43.3 NUCLEAR CALCIUM 43.4
SUMMARY REFERENCES CHAPTER FORTY-FOUR ASSESSMENT OF GAP JUNCTIONAL
INTERCELLULAR COMMUNICATION IN LIVING CELLS USING FLUORESCENCE
TECHNIQUES A.W. DE FEIJTER, IE. TROSKO & M.H. WADE 44.1 44.2 44.3 44.4
INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION ACKNOWLEDGEMENTS
REFERENCES CHAPTER FORTY-FIVE PHOTOACTIVATION OF FLUORESCENCE AS A PROBE
FOR CYTOSKELETAL DYNAMICS IN MITOSIS AND CELL MOTILITY K.E. SAWIN, J.A.
THERIOT & T.J. MITCHISON 45.1 INTRODUCTION 45.2 PHOTOACTIVATABLE
FLUORESCENT PROBES 45.3 A COMPUTER-CONTROLLED, MULTIPLE-CHANNEL
FLUORESCENCE MICROSCOPE FOR PHOTOACTIVATION 45.4 EXPERIMENTS USING
PHOTOACTIVATION OF FLUORESCENCE 45.5 FUTURE PROSPECTS AND CONCLUSIONS
CONTENTS XXV REFERENCES 626 NOTE ADDED IN PROOF 627 CHAPTER FORTY-SIX
VIDEO IMAGING OF LIPID ORDER 628 K FLORINE-CASTEEL, J.L. LEMASTERS & B.
HERMAN 46.1 INTRODUCTION 628 46.2 THEORY 629 46.3 EXPERIMENT 631 46.4
BIOLOGICAL APPLICATIONS 632 ACKNOWLEDGEMENTS 633 REFERENCES 633 INDEX
634 COLOUR PLATES APPEAR BETWEEN PAGES 102 AND 103 (PLATES 5.1 TO 19.1),
AND 326 AND 327 (PLATES 23.1 TO 46.2) AVAILABLE IN THE FIRST EDITION OF
FLUORESCENT AND LUMINESCENT PROBES, ALSO EDITED BY BILL MASON: ( *
PROBES FOR THE ENDOPLASMIC RETICULUM (CHAPTER 8) M. TERASAKI * PROBING
MITOCHONDRIAL MEMBRANE POTENTIAL IN LIVING CELLS BY A
J-AGGREGATE-FORMING DYE (CHAPTER 9) L.B. CHEN & ST. SMILEY * A
SMALL-AREA COOLED CCD CAMERA AND SOFTWARE FOR FLUORESCENCE RATIO IMAGING
(CHAPTER 13) G.J. LAW & W. O BRIEN * MULTIPARAMETE R IMAGING OF CELLULAR
FUNCTION (CHAPTER 14) G.R. BRIGHT * DIGITAL IMAGE ANALYSIS: SOFTWARE
APPROACHES AND APPLICATIONS (CHAPTER 15) G. T RELF * FLOW CYTOMETRIC
ANALYSIS AND SORTING OF VIABLE CELLS (CHAPTER 24) J.F. KEIJ & H.
HERWEIJER
|
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| id | DE-604.BV012639781 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:31:07Z |
| institution | BVB |
| isbn | 0124478360 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008587921 |
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| physical | XXV, 647 S. Ill., graph. Darst. |
| publishDate | 1999 |
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| publisher | Acad. Press |
| record_format | marc |
| series2 | Biological techniques |
| spelling | Fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real-time analysis ed. by W. T. Mason 2. ed. San Diego [u.a.] Acad. Press 1999 XXV, 647 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biological techniques Biologie moléculaire Biologie moléculaire ram Biologische activiteit gtt Biologische markers gtt Colorants fluorescents Cytophotométrie Fluorescentie gtt Luminescent probes Luminescentie gtt Microscopie à fluorescence Sondes fluorescentes ram Spectrometrie gtt Cytophotometry Fluorescent Dyes Fluorescent probes Microscopy, Fluorescence Molecular Biology Durchflusscytometrie (DE-588)4226628-2 gnd rswk-swf Fluoreszenzmarkierung (DE-588)4154829-2 gnd rswk-swf Fluoreszenzmikroskopie (DE-588)4290958-2 gnd rswk-swf Fluoreszenzmarkierung (DE-588)4154829-2 s DE-604 Fluoreszenzmikroskopie (DE-588)4290958-2 s Durchflusscytometrie (DE-588)4226628-2 s Mason, W. T. Sonstige oth HEBIS Datenaustausch Darmstadt application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008587921&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real-time analysis Biologie moléculaire Biologie moléculaire ram Biologische activiteit gtt Biologische markers gtt Colorants fluorescents Cytophotométrie Fluorescentie gtt Luminescent probes Luminescentie gtt Microscopie à fluorescence Sondes fluorescentes ram Spectrometrie gtt Cytophotometry Fluorescent Dyes Fluorescent probes Microscopy, Fluorescence Molecular Biology Durchflusscytometrie (DE-588)4226628-2 gnd Fluoreszenzmarkierung (DE-588)4154829-2 gnd Fluoreszenzmikroskopie (DE-588)4290958-2 gnd |
| subject_GND | (DE-588)4226628-2 (DE-588)4154829-2 (DE-588)4290958-2 |
| title | Fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real-time analysis |
| title_auth | Fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real-time analysis |
| title_exact_search | Fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real-time analysis |
| title_full | Fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real-time analysis ed. by W. T. Mason |
| title_fullStr | Fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real-time analysis ed. by W. T. Mason |
| title_full_unstemmed | Fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real-time analysis ed. by W. T. Mason |
| title_short | Fluorescent and luminescent probes for biological activity |
| title_sort | fluorescent and luminescent probes for biological activity a practical guide to technology for quantitative real time analysis |
| title_sub | a practical guide to technology for quantitative real-time analysis |
| topic | Biologie moléculaire Biologie moléculaire ram Biologische activiteit gtt Biologische markers gtt Colorants fluorescents Cytophotométrie Fluorescentie gtt Luminescent probes Luminescentie gtt Microscopie à fluorescence Sondes fluorescentes ram Spectrometrie gtt Cytophotometry Fluorescent Dyes Fluorescent probes Microscopy, Fluorescence Molecular Biology Durchflusscytometrie (DE-588)4226628-2 gnd Fluoreszenzmarkierung (DE-588)4154829-2 gnd Fluoreszenzmikroskopie (DE-588)4290958-2 gnd |
| topic_facet | Biologie moléculaire Biologische activiteit Biologische markers Colorants fluorescents Cytophotométrie Fluorescentie Luminescent probes Luminescentie Microscopie à fluorescence Sondes fluorescentes Spectrometrie Cytophotometry Fluorescent Dyes Fluorescent probes Microscopy, Fluorescence Molecular Biology Durchflusscytometrie Fluoreszenzmarkierung Fluoreszenzmikroskopie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008587921&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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