Techniques in molecular medicine:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
1999
|
| Schriftenreihe: | Springer lab manual
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 387 S. Ill., graph. Darst. |
| ISBN: | 3540571299 |
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| 245 | 1 | 0 | |a Techniques in molecular medicine |c Friedhelm Hildebrandt ... (eds.) |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 1999 | |
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Datensatz im Suchindex
| _version_ | 1804127287770087424 |
|---|---|
| adam_text | CONTENTS PART I MOLECULAR BIOLOGY TECHNIQUES CHAPTER 1 STRUCTURE AND
FUNCTION OF THE HUMAN GENOME FRIEDHELM HILDEBRANDT . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . 3 CHAPTER 2 ENZYMATIC
MODIFICATION OF DNA AND RNA KAI-OLAF NETZER . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . 24 SUBPROTOCOL 1:
RESTRICTION ENDONUCLEASES . . . . . . . . . . . . . . . . . . . . . 24
SUBPROTOCOL 2: EXONUCLEASES, OTHER NUCLEASES AND LIGASES . . . . . . . .
33 SUBPROTOCOL 3: PHOSPHATASES CIP REACTION . . . . . . . . . . . . .
. . . . . . 36 SUBPROTOCOL 4: KINASES . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . 37 SUBPROTOCOL 5: DNA-DEPENDENT
DNA POLYMERASES E. COLI DNA POLYMERASE 1 AND KLENOW ENZYME . . . . 40
SUBPROTOCOL 6: T4 AND T7 DNA POLYMERASE . . . . . . . . . . . . . . . .
. . . . 42 SUBPROTOCOL 7: RNA-DEPENDENT DNA POLYMERASES . . . . . . . .
. . . . . . 44 SUBPROTOCOL 8: DNA DEPENDENT RNA POLYMERASES . . . . .
. . . . . . . 47 SUBPROTOCOL 9: TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE
(TDT) . . . . . . 49 CHAPTER 3 PURIFICATION AND NUCLEIC ACIDS FROM
EUKARYOTIC CELLS HANS GERD NOTHWANG AND FRIEDHELM HILDEBRANDT . . . . .
. . . 50 SUBPROTOCOL 1: EXTRACTION OF NUCLEIC ACIDS . . . . . . . . . .
. . . . . . . . . . 50 SUBPROTOCOL 2: ISOLATION OF GENOMIC DNA FROM
MAMMALIAN CELLS . . 56 SUBPROTOCOL 3: ISOLATION OF CYTOPLASMIC RNA . . .
. . . . . . . . . . . . . . . 60 SUBPROTOCOL 4: RAPID PREPARATION OF
TOTAL RNA BY ACID GUANIDINIUM THIOCYANATE/PHENOL/CHLOROFORM/ EXTRACTION
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
SUBPROTOCOL 5: PREPARATION OF POLY(A) + RNA . . . . . . . . . . . . . .
. . . . . 67 CHAPTER 4 ELECTROPHORESIS OF DNA AND RNA FRAGMENTS FENG
QIAN AND GREGORY G. GERMINO . . . . . . . . . . . . . . . . . . . . . .
71 SUBPROTOCOL 1: THE RECOVERY OF DNA FRAGMENTS . . . . . . . . . . . .
. . . . 75 SUBPROTOCOL 2: ELECTROPHORESIS OF RNA . . . . . . . . . . . .
. . . . . . . . . . . 77 CHAPTER 5 SINGLE-STRAND CONFORMATION
POLYMORPHISM (SSCP) ANALYSIS FENG QIAN AND GREGORY G. GERMINO . . . . .
. . . . . . . . . . . . . . . . . 83 CHAPTER 6 DENATURING GRADIENT GEL
ELECTROPHORESIS KAI-OLAF NETZER . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . 86 CHAPTER 7 INTRODUCTION OF
PULSED-FIELD GEL ELECTROPHORESIS (PFGE) FENG QIAN AND GREGORY G. GERMINO
. . . . . . . . . . . . . . . . . . . . . . 96 CHAPTER 8 PULSED-FIELD
GEL ELECTROPHORESIS: PROTOCOLS GUDRUN A. RAPPOLD, KARIN RIED, ALBRECHT
KLINK, ERCOLE RAO AND BIRGIT WEISS . . . . . . . . . . . . . . . . . . .
. . . . . . . . . 103 SUBPROTOCOL 1: PREPARATION AND PROCESSING OF HIGH
MOLECULAR DNA IN AGAROSE BLOCKS . . . . . . . . . . . . . . . . . . . .
. . 104 SUBPROTOCOL 2: PREPARATION OF SIZE MARKERS . . . . . . . . . . .
. . . . . . . . . 106 SUBPROTOCOL 3: RESTRICTION ENZYME DIGESTION OF DNA
IN AGAROSE BLOCKS . . . . . . . . . . . . . . . . . . . . . . . . . .
108 SUBPROTOCOL 4: GEL ELECTROPHORESIS . . . . . . . . . . . . . . . . .
. . . . . . . . . 110 CHAPTER 9 RADIOACTIVE LABELING OF DNA AND RNA
PROBES PETER IGARASHI . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . 114 SUBPROTOCOL 1: END-LABELING USING T4
POLYNUCLEOTIDE KINASE . . . . . . 114 SUBPROTOCOL 2: END-LABELING USING
THE EXCHANGE KINASE REACTION . . 116 SUBPROTOCOL 3: LABELING 3 ENDS
WITH TERMINAL TRANSFERASE (FORWARD REACTION) . . . . . . . . . . . . . .
. . . . . . . . . . . 117 SUBPROTOCOL 4: RANDOM-PRIME LABELING . . . . .
. . . . . . . . . . . . . . . . . 118 SUBPROTOCOL 5: RIBOPROBES . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 VIII
CONTENTS CONTENTS IX CHAPTER 10 HYBRIDIZATION METHODS (SOUTHERN AND
NORTHERN BLOTTING) KAI-OLAF NETZER . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . 126 SUBPROTOCOL 1: PREPARATION
OF DOT BLOTS FOR DNA . . . . . . . . . . . . . . . 129 SUBPROTOCOL 2:
PREPARATION OF SOUTHERN BLOTS FOR GENOMIC DNA . . . 131 SUBPROTOCOL 3:
HYBRIDIZATION OF DNA BLOTS . . . . . . . . . . . . . . . . . . . . 135
SUBPROTOCOL 4: PREPARATION OF RNA DOT BLOTS . . . . . . . . . . . . . .
. . . . 139 SUBPROTOCOL 5: PREPARATION OF NORTHERN BLOTS . . . . . . . .
. . . . . . . . . . 140 SUBPROTOCOL 6: HYBRIDIZATION OF RNA BLOTS . . .
. . . . . . . . . . . . . . . . . 143 SUBPROTOCOL 7: MEMBRANE STRIPPING
. . . . . . . . . . . . . . . . . . . . . . . . . 147 CHAPTER 11
SPECIMEN PREPARATION TECHNIQUES FOR CYTOGENETIC ANALYSIS BY FLUORESCENCE
IN SITU HYBRIDIZATION STEFAN JOOS, MARTIN BENTZ, ANTON H. N. HOPMAN AND
PETER LICHTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . 148 SUBPROTOCOL 1: CULTIVATION OF BLOOD LYMPHOCYTES . .
. . . . . . . . . . . . . 149 SUBPROTOCOL 2: PREPARATION OF METAPHASE
CHROMOSOMES AND INTERPHASE NUCLEI FROM BLOOD LYMPHOCYTES . . . . 151
SUBPROTOCOL 3: PEPSIN DIGESTION OF BLOOD LYMPHOCYTES . . . . . . . . . .
153 SUBPROTOCOL 4: PREPARATION OF FIBROBLASTS FOR FISH (ADHERENTLY
GROWING CELLS) . . . . . . . . . . . . . . . . . . . . 154 SUBPROTOCOL
5: TREATMENT OF BLOOD SMEARS . . . . . . . . . . . . . . . . . . . . 155
SUBPROTOCOL 6: BONE MARROW SMEARS . . . . . . . . . . . . . . . . . . .
. . . . . . 156 SUBPROTOCOL 7: CLINICAL MATERIAL OBTAINED FROM SOLID
TUMORS: PRETREATMENT OF SLIDES WITH POLY-L-LYSINE . . . . . . . . . 157
SUBPROTOCOL 8: PREPARATION OF SINGLE CELL SUSPENSIONS FROM SOLID TUMOR
MATERIAL . . . . . . . . . . . . . . . . . . . . 158 SUBPROTOCOL 9:
PREPARATION OF FROZEN SECTIONS . . . . . . . . . . . . . . . . . 160
SUBPROTOCOL 10: PARAFFIN-EMBEDDED TISSUE SECTIONS . . . . . . . . . . .
. . 161 CHAPTER 12 CHROMOSOME ANALYSIS BY FLUORESCENCE IN SITU
HYBRIDIZATION MARTIN BENTZ, STEFAN JOOS AND PETER LICHTER . . . . . . .
. . . . . . 166 SUBPROTOCOL 1: SPECIMEN PREPARATION . . . . . . . . . .
. . . . . . . . . . . . . . 170 SUBPROTOCOL 2: PROBE LABELLING BY NICK
TRANSLATION . . . . . . . . . . . . . 171 SUBPROTOCOL 3: DOT-BLOT ASSAY
TO TEST LABELLING . . . . . . . . . . . . . . . . 174 SUBPROTOCOL 4:
PROBE MIXTURE AND DENATURATION FOR FLUORESCENCE IN SITU HYBRIDIZATION .
. . . . . . . . . . . . . . . . . . . . . . . . 175 X CONTENTS
SUBPROTOCOL 5: DENATURATION OF THE DNA ON THE SLIDES . . . . . . . . . .
. 176 SUBPROTOCOL 6: DETECTION . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . 178 SUBPROTOCOL 7: CHROMOSOMAL IN SITU
SUPPRESSION (CISS) HYBRIDIZATION . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . 179 SUBPROTOCOL 8: SIGNAL AMPLIFICATION . . . . .
. . . . . . . . . . . . . . . . . . . . . 181 SUBPROTOCOL 9: MULTICOLOR
FLUORESCENCE IN SITU HYBRIDIZATION . . . . . . 182 CHAPTER 13 SEQUENCING
KLAUS DEICHMANN . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . 186 SUBPROTOCOL 1: STANDARD PROTOCOL FOR T7 DNA
POLYMERASE (SEQUENASE TM ) . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . 194 SUBPROTOCOL 2: STANDARD PROTOCOL FOR SEQUENCING WITH
TAQ POLYMERASE . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . 197 SUBPROTOCOL 3: DENATURING POLYACRYLAMIDE SEQUENCING GELS . .
. . . . . 200 CHAPTER 14 POLYMERASE CHAIN REACTION FRIEDHELM HILDEBRANDT
AND IVA SINGH-SAWHNEY . . . . . . . . . . 207 CHAPTER 15 CLONING VECTORS
ANNETTE DEICHMANN AND KLAUS DEICHMANN . . . . . . . . . . . . . . . 226
SUBPROTOCOL 1: TRANSFORMATION OF BACTERIA WITH RECOMBINANT PLASMID USING
THE CA ++ -PRECIPITATION METHOD (MANDEL 1970) . . . . . . . . . . . . .
. . . . . . . . . . . . . . . . . 234 SUBPROTOCOL 2: TRANSFORMATION OF
BACTERIA WITH RECOMBINANT PLASMID BY ELECTROPORATION (DOWER 1988) . . .
. . . . . . 238 SUBPROTOCOL 3: TRANSFORMATION OF BACTERIA WITH
RECOMBINANT BACTERIOPHAGE DNA . . . . . . . . . . . . . . . . . . . . .
. . . . . 241 SUBPROTOCOL 4: MINI-PREP METHOD FOR PLASMID ISOLATION .
. . . . . . . 245 SUBPROTOCOL 5: CHROMATOGRAPHIC METHOD FOR PLASMID
PREPARATION . . 247 SUBPROTOCOL 6: LARGE-SCALE PREPARATION OF
BACTERIOPHAGE DNA (REPLICATIVE FORM (RF)) . . . . . . . . . . . . . . .
. . . . . . . . 249 CONTENTS XI PART II STRATEGIES FOR GENE ISOLATION
AND CHARACTERIZATION CHAPTER 16 CLONING STRATEGIES: AN OVERVIERW
FRIEDHELM HILDEBRANDT . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . 257 CHAPTER 17 GENOMIC LIBRARIES LUITZ F. ONUCHIC AND
GREGORY G. GERMINO . . . . . . . . . . . . . . . . 261 CHAPTER 18 CDNA
LIBRARIES LUITZ F. ONUCHIC AND GREGORY G. GERMINO . . . . . . . . . . .
. . . . . 273 CHAPTER 19 CDNA LIBRARY SCREENING JOHANN PETER HOSSLE . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 288
SUBPROTOCOL 1: PLATING OF A K GT11 CDNA LIBRARY . . . . . . . . . . . .
. . . . 296 SUBPROTOCOL 2: HYBRIDIZATION SCREENING - CDNA SEQUENCES AS
PROBES 298 SUBPROTOCOL 3: IMMUNO-SCREENING . . . . . . . . . . . . . . .
. . . . . . . . . . . . 300 SUBPROTOCOL 4: PURIFICATION OF PHAGE
PARTICLES, DNA ISOLATION AND SUBCLONING . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . 306 CHAPTER 20 EXPRESSION CLONING WITH XENOPUS
OOCYTES DANIEL MARKOVICH, ANDREAS WERNER AND HEINI MURER . . . . . . 310
CHAPTER 21 TRANSGENIC ANIMALS ECKART SCHOTT, MARTIN PAUL AND DETLEV
GANTEN . . . . . . . . . . 319 CHAPTER 22 EXPRESSION IN EUKARYOTIC
CELLS: GENE TRANSFER USING RETROVIRAL VECTORS MARTIN MARX . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
337 SUBPROTOCOL 1: SYNTHESIS OF THE INSERT . . . . . . . . . . . . . . .
. . . . . . . . . 339 SUBPROTOCOL 2: PURIFICATION . . . . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . 340 SUBPROTOCOL 3: LIGATION OF
THE INSERT INTO THE VECTOR . . . . . . . . . . . . 340 SUBPROTOCOL 4:
TRANSFECTION OF E. COLI HB101 AND PLASMID DNA ISOLATION . . . . . . . .
. . . . . . . . . . . . . . . . . . . . . . 341 SUBPROTOCOL 5: ASSAY FOR
THE PRESENCE OF THE DNA INSERT . . . . . . . . . 343 XII CONTENTS
SUBPROTOCOL 6: TRANSFECTION OF W 2 PACKAGING CELLS . . . . . . . . . . .
. . . 344 SUBPROTOCOL 7: INFECTION OF W AM PACKAGING CELLS . . . . . . .
. . . . . . . . 348 SUBPROTOCOL 8: INFECTION OF TARGET CELLS . . . . . .
. . . . . . . . . . . . . . . . . 348 CHAPTER 23 POSITIONAL CLONING AND
LINKAGE ANALYSIS FRIEDHELM HILDEBRANDT AND HEYMUT OMRAN . . . . . . . .
. . . . . . 352 PART III PROTEIN METHODS CHAPTER 24 PROTEIN TECHNIQUES
ROBERT F. REILLY . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . 367 SUBPROTOCOL 1: IN VITRO TRANSLATION AND
IMMUNOPRECIPITATION . . . . . 367 SUBPROTOCOL 2: WESTERN BLOTTING . . .
. . . . . . . . . . . . . . . . . . . . . . . . . 375 SUBJECT INDEX . .
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . . . 379
|
| any_adam_object | 1 |
| author_GND | (DE-588)120990954 |
| building | Verbundindex |
| bvnumber | BV012631220 |
| callnumber-first | Q - Science |
| callnumber-label | QH506 |
| callnumber-raw | QH506 |
| callnumber-search | QH506 |
| callnumber-sort | QH 3506 |
| callnumber-subject | QH - Natural History and Biology |
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| classification_tum | BIO 220f |
| ctrlnum | (OCoLC)41326205 (DE-599)BVBBV012631220 |
| dewey-full | 572.8 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8 |
| dewey-search | 572.8 |
| dewey-sort | 3572.8 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Book |
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| id | DE-604.BV012631220 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:30:57Z |
| institution | BVB |
| isbn | 3540571299 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008581477 |
| oclc_num | 41326205 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-20 DE-19 DE-BY-UBM DE-29 DE-91G DE-BY-TUM DE-188 DE-578 |
| owner_facet | DE-355 DE-BY-UBR DE-20 DE-19 DE-BY-UBM DE-29 DE-91G DE-BY-TUM DE-188 DE-578 |
| physical | XII, 387 S. Ill., graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | Springer |
| record_format | marc |
| series2 | Springer lab manual |
| spelling | Techniques in molecular medicine Friedhelm Hildebrandt ... (eds.) Berlin [u.a.] Springer 1999 XII, 387 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Springer lab manual Molecular Biology cabt Techniques cabt Laboratoriumonderzoek gtt Moleculaire biologie gtt Medical genetics Laboratory manuals Molecular Biology methods Laboratory Manuals Molecular biology Laboratory manuals Molecular diagnosis Laboratory manuals Methode (DE-588)4038971-6 gnd rswk-swf Medizin (DE-588)4038243-6 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Medizin (DE-588)4038243-6 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s DE-604 Hildebrandt, Friedhelm 1957- Sonstige (DE-588)120990954 oth SWB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008581477&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Techniques in molecular medicine Molecular Biology cabt Techniques cabt Laboratoriumonderzoek gtt Moleculaire biologie gtt Medical genetics Laboratory manuals Molecular Biology methods Laboratory Manuals Molecular biology Laboratory manuals Molecular diagnosis Laboratory manuals Methode (DE-588)4038971-6 gnd Medizin (DE-588)4038243-6 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4038243-6 (DE-588)4039983-7 |
| title | Techniques in molecular medicine |
| title_auth | Techniques in molecular medicine |
| title_exact_search | Techniques in molecular medicine |
| title_full | Techniques in molecular medicine Friedhelm Hildebrandt ... (eds.) |
| title_fullStr | Techniques in molecular medicine Friedhelm Hildebrandt ... (eds.) |
| title_full_unstemmed | Techniques in molecular medicine Friedhelm Hildebrandt ... (eds.) |
| title_short | Techniques in molecular medicine |
| title_sort | techniques in molecular medicine |
| topic | Molecular Biology cabt Techniques cabt Laboratoriumonderzoek gtt Moleculaire biologie gtt Medical genetics Laboratory manuals Molecular Biology methods Laboratory Manuals Molecular biology Laboratory manuals Molecular diagnosis Laboratory manuals Methode (DE-588)4038971-6 gnd Medizin (DE-588)4038243-6 gnd Molekularbiologie (DE-588)4039983-7 gnd |
| topic_facet | Molecular Biology Techniques Laboratoriumonderzoek Moleculaire biologie Medical genetics Laboratory manuals Molecular Biology methods Laboratory Manuals Molecular biology Laboratory manuals Molecular diagnosis Laboratory manuals Methode Medizin Molekularbiologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008581477&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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