Verfügbarkeit: Diagnostic cytogenetics

Diagnostic cytogenetics:

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Bibliographische Detailangaben
Format:	Buch
Sprache:	English
Veröffentlicht:	Berlin [u.a.] Springer 1999
Schriftenreihe:	Springer lab manual
Schlagworte:	Cytogenetik - Cytodiagnostik Cytodiagnostik Diagnosesystem Cytogenetik
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XIV, 460 S. Ill., graph Darst.
ISBN:	3540646027

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MARC


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Datensatz im Suchindex

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adam_text	Part I Classical Cytogenetics Chapter 1 Tissue Culture FRIEDEL WENZEL 3 Subprotocol 1: Sampling, Transport, Storage 20 Subprotocol 2: Ciyopreservation 26 Subprotocol 3: Bacterial and/or fungal contamination 33 Subprotocol 4: Mycoplasma Contamination 37 Subprotocol 5: Elimination of Mycoplasmas from Cell Cultures .... 45 Chapter 2 Chromosome Staining ANGELIKA KOHLER 52 Subprotocol 1: Giemsa Staining 55 Subprotocol 2: GTG Banding 56 Subprotocol 3: QFQ Banding 59 Subprotocol 4: C Banding 60 Subprotocol 5: NOR Staining 62 Subprotocol 6: DA DAPI Staining 64 Subprotocol 7: Replication Pattern (by BrdU Incorporation) 65 Subprotocol 8: Sister Chromatid Differentiation (by BrdU Incorporation) 68 Subprotocol 9: Sex Chromatin Staining X Chromatin (Barr Body) . 70 Subprotocol 10: Sex Chromatin Staining Y Chromatin (Y Body) . . 72 Chapter 3 Karyotyping and Data Interpretation KARSTEN HELD 75 Chapter 4 Documentation PETER MINY AND ROLF DIETER WEGNER 96 Part II Postnatal Diagnosis Chapter 5 Peripheral Blood IRIS BARTELS 115 Chapter 6 Establishment of Permanent Growing Lymphoblastoid Cell Lines HEIDEMARIE NEITZEL 121 Subprotocol 1: Transformation of B Lymphocytes with B95 8 EB Virus 123 Subprotocol 2: Ficoll Separation of Unfractionated Mononuclear Leukocytes Obtained from Whole Blood 125 Subprotocol 3: Establishment of Cultures 126 Subprotocol 4: Freezing of Lymphoblastoid Cells 127 Subprotocol 5: Thawing of Lymphoblastoid Cells 128 Subprotocol 6: Chromosome Preparations from Lymphoblastoid Cells 129 Chapter 7 Solid Tissues REGINE SCHUBERT AND GESA SCHWANITZ 132 Chapter 8 Cells from Urine Sample HANNELORE KORNER, HENRIKE DIA AND CHRISTIANE BOMMER ... 142 Chapter 9 Classical and Molecular Cytogenetics of Tumor Cells BRIGITTE SCHLEGELBERGER, SIMONE METZKE, SVETLANA HARDER, REINA ZUHLKE JENISCH.YANMING ZHANG, REINER SIEBERT 151 Subprotocol 1: Chromosome Analysis 152 Subprotocol 2: Fluorescence in Situ Hybridization 168 Chapter 10 Cytogenetics of Meiotic Cells R. JOHANNISSON 186 Subprotocol 1: Air Drying Method 188 Subprotocol 2: Surface Spreading Method Using Light Microscopy . . 190 Subprotocol 3: Surface Spreading Using Electron Microscopy 199 Subprotocol 4: Ejaculate 207 Part III Prenatal Diagnosis Chapter 11 Prenatal Diagnosis An Introduction ROLF DIETER WEGNER 213 Chapter 12 Amniotic Fluid Cell Analysis INGO KENNERKNECHT, MAHMOUD DJALALI, GOTTHOLD BARBI, WALTER JUST AND WALTHER VOGEL 217 Chapter 13 Chorionic Villi Sampling ROLF DIETER WEGNER AND HOLGER TOENNIES 231 Subprotocol 1: Setting up a Short Term Culture 235 Subprotocol 2: Chromosome Preparation from Short Term Culture 238 Subprotocol 3: Setting up a Long Term Culture by Physical Maceration 239 Subprotocol 4: Setting up a Long Term Culture by Enzymatic Dissociation 241 Subprotocol 5: Cell Harvest and Chromosome Preparation 242 Part IV Special Applications Chapter 14 Diagnosis of Chromosomal Instability Syndromes ROLF DIETER WEGNER AND MARKUS STUMM 251 Chapter 15 Flow Cytometric Testing for Syndromes with Chromosomal Instability, Aplastic Anemia and Related Hematological Disorders DETLEV SCHINDLER AND HOLGER HOEHN 269 Chapter 16 Cell Fusion MARKUS STUMM AND ROLF DIETER WEGNER 282 Chapter 17 Origin of Trisomies GESA SCHWANITZ AND THOMAS EGGERMANN 291 Part V Molecular Cytogenetics Chapter 18 Fluorescence in Situ Hybridization GESA SCHWANITZ AND REGINE SCHUBERT 305 Subprotocol 1: Preparation of Slides 310 Subprotocol 2: Labelling of DNA Probes 313 Subprotocol 3: Denaturation and Hybridization 317 Subprotocol 4: Detection of Bio tin Labelled Probes 319 Subprotocol 5: Amplification 322 Subprotocol 6: Simultaneous Two Colour Detection 323 Subprotocol 7: In Situ Hybridization with Unique Sequence Probes . 324 Subprotocol 8: Preparation of Interphase Nuclei 326 Chapter 19 Fluorescence in Situ Hybridization (FISH) Analysis in Human Sperm Cells EVELYN KO AND RENEE MARTIN 335 Chapter 20 Microdissection and Reverse Chromosome Painting GABRIELE SENGER, JORG WEIMER, UWE CLAUSSEN, AND ILSE CHUDOBA 356 Chapter 21 Comparative genomic hybridisation (CGH) TRAUDL HENN AND OSKAR A. HAAS 376 Part VI Techniques in Development Chapter 22 Fetal Cells in Maternal Blood DOROTHEE GANSHIRT, HENK S.P. GARRITSEN, WOLFGANG HOLZGREVE 401 Chapter 23 Spectral Karyotyping in Clinical and Tumor Cytogenetics EVELIN SCHROCK, YUVAL GARINI, MICHAEL KOHLER AND THOMAS RIED 416 Chapter 24 Chromosome Analysis by Multiplex FISH (M FISH) MICHAEL R. SPEICHER 439 Subject Index 457
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discipline	Biologie Medizin
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isbn	3540646027
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spelling	Diagnostic cytogenetics Rolf-Dieter Wegner (ed.) Berlin [u.a.] Springer 1999 XIV, 460 S. Ill., graph Darst. txt rdacontent n rdamedia nc rdacarrier Springer lab manual Cytogenetik - Cytodiagnostik Cytodiagnostik (DE-588)4070174-8 gnd rswk-swf Diagnosesystem (DE-588)4149458-1 gnd rswk-swf Cytogenetik (DE-588)4070176-1 gnd rswk-swf Cytogenetik (DE-588)4070176-1 s Diagnosesystem (DE-588)4149458-1 s DE-604 Cytodiagnostik (DE-588)4070174-8 s Wegner, Rolf-Dieter 1949- Sonstige (DE-588)12087461X oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008563528&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Diagnostic cytogenetics Cytogenetik - Cytodiagnostik Cytodiagnostik (DE-588)4070174-8 gnd Diagnosesystem (DE-588)4149458-1 gnd Cytogenetik (DE-588)4070176-1 gnd
subject_GND	(DE-588)4070174-8 (DE-588)4149458-1 (DE-588)4070176-1
title	Diagnostic cytogenetics
title_auth	Diagnostic cytogenetics
title_exact_search	Diagnostic cytogenetics
title_full	Diagnostic cytogenetics Rolf-Dieter Wegner (ed.)
title_fullStr	Diagnostic cytogenetics Rolf-Dieter Wegner (ed.)
title_full_unstemmed	Diagnostic cytogenetics Rolf-Dieter Wegner (ed.)
title_short	Diagnostic cytogenetics
title_sort	diagnostic cytogenetics
topic	Cytogenetik - Cytodiagnostik Cytodiagnostik (DE-588)4070174-8 gnd Diagnosesystem (DE-588)4149458-1 gnd Cytogenetik (DE-588)4070176-1 gnd
topic_facet	Cytogenetik - Cytodiagnostik Cytodiagnostik Diagnosesystem Cytogenetik
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008563528&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
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