DNA topoisomerase protocols: 1 DNA topology and enzymes
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1999
|
| Schriftenreihe: | Methods in molecular biology
94 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 327 S. Ill., graph. Darst. |
| ISBN: | 0896034445 |
Internformat
MARC
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Datensatz im Suchindex
| _version_ | 1804127244788957184 |
|---|---|
| adam_text | Contents
Preface v
Contents of Companion Volume xi
Contributors xiii
1 Introduction to DNA Topoisomerases
Mary Ann Bjornsti and Neil Osheroff 1
2 Resolution of DNA Molecules by One Dimensional Agarose Gel
Electrophoresis
Mary Ann Bjornsti and Maureen D. Megonigal 9
3 Two Dimensional Agarose Gel Electrophoresis of DNA Topoisomers
Ryo Hanai and Joaquim Roca 19
4 Analysis of Altered DNA Structures: Cruciform DNA
Albert J. Courey 29
5 Purification of Supercoiled Plasmid DNA
Kelly L. Jordan, Devon L. Evans, and David J. Hall 41
6 Purification and Use of DNA Minicircles with Different Linking
Numbers
Giorgio Camilloni, Rodolfo Negri, Micaela Caserta,
and Ernesto Di Mauro 51
7 Isolation of Kinetoplast DNA
Theresa A. Shapiro, Viiu A. Klein, and Paul T. Englund 61
8 Isolation of Knotted DNA from Coliphage P4
Morten Isaksen, Bryan Julien, Richard Calendar,
and Bjorn H. Lindqvist 69
9 Analysis of DNA Knots and Catenanes by Agarose Gel
Electrophoresis
Stephen D. Levene and Hua Tsen 75
10 Sedimentation Analysis of Bacterial Nucleoid Structure
Karl Drlica, Chang Rung Chen, and Samuel Kay man 87
11 Coating DNA with RecA Protein to Distinguish DNA Path
by Electron Microscopy
E. Lynn Zechiedrich and Nancy J. Crisona 99
wii
via Contents
12 Methods for Analyzing DNA Bending
Jason D. Kahn 109
13 Formation of Extrachromosomal DNA Rings in Saccharomyces
cerevisiae Using Site Specific Recombination
Marc R. Gartenburg 125
14 Overexpression and Purification of Bacterial DNA Gyrase
Anthony Maxwell and Alison J. Howells 135
15 Overexpression and Purification of Bacterial DNA
Topoisomerase I
Chang Xi Zhu and Yuk Ching Tse Dinh 145
16 Overexpression and Purification of Escherichia coli DNA
Topoisomerase III
Russell J. DiGate 153
17 Overexpression and Purification of Bacterial Topoisomerase IV
Hong Peng and Kenneth J. Marians 163
18 Purification of the Bacteriophage T4 Type II DNA Topoisomerase
Kenneth N. Kreuzer and Sue H. Neece 171
19 Overexpression and Purification of DNA Topoisomerase I
from Yeast
Mary Ann Bjornsti and Jolanta Fertala 179
20 Overexpression and Purification of Saccharomyces cerevisiae
DNA Topoisomerase II from Yeast
Janet E. Lindsley 187
21 Purification of DNA Topoisomerase II from Drosophila
melanogaster
Stacie J. Froelich Ammon, Paul S. Kingma, and Neil Osheroff 199
22 Purification of DNA Topoisomerase I from Human Placenta
Joseph A. Holden 213
23 Purification of Baculovirus Expressed Human DNA
Topoisomerase I
Lance Stewart and James J. Champoux 223
24 Rapid Purification of DNA Topoisomerase II Containing
a Hexahistidine Tag by Metal Ion Affinity Chromatography
Harald Biersack, Sanne Jensen, and Ole Westergaard 235
25 Metabolic Labeling, Immunoprecipitation, and Two Dimensional
Tryptic Phosphopeptide Mapping of Human Topoisomerase II
Nicholas J. Wells and Ian D. Hickson 243
26 Immunoblot Analysis and Band Depletion Assays
Scott H. Kaufmann and Phyllis A. Svingen 253
Contents ix
27 Visualization of DNA Topoisomerases by Electron Microscopy
Piero Benedetti and Alessandra Silvestri 269
28 Appendix: Compendium of DNA Topoisomerase Sequences
Paul R. Caron 279
Index 317
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| indexdate | 2024-07-09T18:30:16Z |
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| isbn | 0896034445 |
| language | English |
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| physical | XV, 327 S. Ill., graph. Darst. |
| publishDate | 1999 |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | DNA topoisomerase protocols 1 DNA topology and enzymes ed. by Mary-Ann Bjornsti ... Totowa, NJ Humana Press 1999 XV, 327 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 94 Methods in molecular biology ... Methode (DE-588)4038971-6 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf DNS-Gyrase (DE-588)4285125-7 gnd rswk-swf DNS-Gyrase (DE-588)4285125-7 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s DE-604 Bjornsti, Mary-Ann Sonstige oth Osheroff, Neil Sonstige oth (DE-604)BV012594847 1 Methods in molecular biology 94 (DE-604)BV035362695 94 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008553041&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | DNA topoisomerase protocols Methods in molecular biology Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd DNS-Gyrase (DE-588)4285125-7 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4039983-7 (DE-588)4285125-7 |
| title | DNA topoisomerase protocols |
| title_auth | DNA topoisomerase protocols |
| title_exact_search | DNA topoisomerase protocols |
| title_full | DNA topoisomerase protocols 1 DNA topology and enzymes ed. by Mary-Ann Bjornsti ... |
| title_fullStr | DNA topoisomerase protocols 1 DNA topology and enzymes ed. by Mary-Ann Bjornsti ... |
| title_full_unstemmed | DNA topoisomerase protocols 1 DNA topology and enzymes ed. by Mary-Ann Bjornsti ... |
| title_short | DNA topoisomerase protocols |
| title_sort | dna topoisomerase protocols dna topology and enzymes |
| topic | Methode (DE-588)4038971-6 gnd Molekularbiologie (DE-588)4039983-7 gnd DNS-Gyrase (DE-588)4285125-7 gnd |
| topic_facet | Methode Molekularbiologie DNS-Gyrase |
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