Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | German |
| Veröffentlicht: |
1998
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 214 S. Ill., graph. Darst. |
Internformat
MARC
| LEADER | 00000nam a2200000 c 4500 | ||
|---|---|---|---|
| 001 | BV012455121 | ||
| 003 | DE-604 | ||
| 005 | 20030314 | ||
| 007 | t | ||
| 008 | 990315s1998 ad|| m||| 00||| gerod | ||
| 035 | |a (OCoLC)246436369 | ||
| 035 | |a (DE-599)BVBBV012455121 | ||
| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a ger | |
| 049 | |a DE-19 |a DE-29T |a DE-355 |a DE-12 |a DE-11 |a DE-188 | ||
| 100 | 1 | |a Karnauchov, Ivan |d 1970- |e Verfasser |0 (DE-588)12098346X |4 aut | |
| 245 | 1 | 0 | |a Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins |c von Ivan Karnauchov |
| 264 | 1 | |c 1998 | |
| 300 | |a 214 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Univ., Diss., 1998 | ||
| 650 | 0 | 7 | |a Membranproteine |0 (DE-588)4130026-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Protein-Sortierung |0 (DE-588)4533502-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Chloroplast |0 (DE-588)4147821-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Thylakoidmembran |0 (DE-588)4185376-3 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Chloroplast |0 (DE-588)4147821-6 |D s |
| 689 | 0 | 1 | |a Thylakoidmembran |0 (DE-588)4185376-3 |D s |
| 689 | 0 | 2 | |a Membranproteine |0 (DE-588)4130026-9 |D s |
| 689 | 0 | 3 | |a Protein-Sortierung |0 (DE-588)4533502-3 |D s |
| 689 | 0 | |5 DE-604 | |
| 856 | 4 | 2 | |m GBV Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008451658&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 940 | 1 | |n oe | |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-008451658 | ||
Datensatz im Suchindex
| _version_ | 1804127093320056832 |
|---|---|
| adam_text | MECHANISMS OF INTRACHLOROPLAST SORTING OF INTEGRAL THYLAKOID MEMBRANE
PROTEINS DISSERTATION ZUR ERLANGUNG DES DOKTORGRNDCS DCR FAKULTIIT FUR
BIOLOGIC DER LUDWIG-MAXIMILIANS-UNIVERSITSIT MIINCHCN VORGELCGT VON IVAN
KARNAUCHOV AUS MOSKAU MUNCHEN 1998 CONTENTS CONTENTS ABBREVIATIONS 7
INTRODUCTION 10 1. PRINCIPLES AND MECHANISMS OF PROTEIN MEMBRANE
TRANSLOCATION 10 I. PROTEIN TRANSLOCATION IN BACTERIAL CELLS 12 II.
PROTEIN TRANSLOCATION AT THE ER MEMBRANE 14 HI. PROTEIN TRANSLOCATION IN
MITOCHONDRIA 16 IV. PROTEIN IMPORT INTO CHLOROPLASTS 20 V. PROTEIN
TRANSLOCATION ACROSS AND INTO THE THYLAKOID MEMBRANE 23 2. STRUCTURAL
AND FUNCTIONAL ORGANIZATION OF THE PHOTOSYNLHETIC APPARATUS OF THE
THYLAKOID MEMBRANE 29 MATERIALS AND METHODS 34 1. MATERIALS 34 1.1.
BACTERIAL STRAINS 34 1.2. VECTORS 34 1.3. PLASMIDS 34 1.4. PLANT
MATERIAL 34 1.5. CHEMICALS, ENZYMES, OLIGONUCLEOTIDES AND OTHER
MATERIALS 34 2. METHODS 35 2.1. STANDARD METHODS 35 2.2 CLONING AND
MUTAGENESIS 35 2.3. PRODUCTION OF PROTEINS IN E. COLI 37 2.3.1.
PREPARATIVE OVEREXPRESSION 37 2.3.2. SYNTHESIS OF RADIOACTIVELY LABELLED
PROTEINS IN E.COLI 38 2.4. TRANSCRIPTION AND TRANSLATION 40 2.4.1. IN
VITRO TRANSCRIPTION 40 2.4.2. IN VITRO TRANSLATION 41 2.5. ISOLATION OF
CHLOROPLASTS AND IMPORT IN ORGANELLO 42 CONTENTS 2.5.1. ISOLATION OF
CHLOROPLASTS FROM SPINACH LEAVES 42 2.5.2. ISOLATION OF INTACT
CHLOROPLASTS FROM PEA LEAVES 43 2.5.3. IMPORT IN ORGANELLO 43 2.5.4.
IMPORT EXPERIMENTS WITH ISOLATED THYLAKOIDS 45 2.5.5. IMPORT EXPERIMENTS
WITH TRYPSIN-TREATCD THYLAKOIDS 46 2.6. RECONSTITUTION ASSAYS 47 2.7.
IMMUNOPRECIPITATION 48 2.8. PROTEASE PROTECTION ASSAYS 49 2.9. PROTEIN
EXTRACTION FROM THYLAKOID MEMBRANE 49 2.10. ELCCLROPHORCSIS OF PROTEINS
50 2.10.1. SDS ELECTROPHORESIS OF PROTEINS 50 2.10.2. NON-DENATURING
ELECTROPHORESIS OF PROTEINS 50 A. SEPARATION OF SOLUBLE PROTEINS (PORE
SIZE EXCLUSION GEL ELECTROPHORESIS) 51 I). SEPARATION OF MEMBRANE
PROTEINS (BLUE NATIVE ELECTROPHORESIS) 52 2.11. DETECTION OF PROTEINS 53
2.11.1. STAINING OF PROTEINS 53 2.11.2. IMMUNODETECTION OF PROTEINS 54
2.12. PREPARATION OF PROTEINS FOR MICROSEQUENCING 55 RESULTS 57 1.
ASSAYS EMPLOYED IN STUDIES ON THYLAKOID PROTEIN TRANSLOCATION AND THEIR
USE FOR THE ANALYSIS OF THE MECHANISM OF MEMBRANE INTEGRATION OF CFOI 1
57 1.1. USE OF METABOLIC INHIBITORS IN THE ANALYSIS OF THE TRANSPORT
MECHANISM OF A PROTEIN 57 1.2. COMPETITION EXPERIMENTS PROVIDE A
RELIABLE MEANS TO DISCRIMINATE BETWEEN THE PROTEIN TRANSLOCATION
PATHWAYS 62 1.3. PROTEASE PRETREATMENT OF THYLAKOIDS PREVENTS THE
PROTEIN TRANSPORT BY THE SEC- AND THE APH-DEPENDENT MECHANISMS, BUT NOT
THE MEMBRANE INTEGRATION OF CFOI 1 63 1.4. APH-DEPENDENT BUT NOT
SEC-DEPENDENT TARGETING SIGNALS CAN ALTER THE MECHANISM OF THYLAKOID
INTEGRATION OF CFOII 65 1.5. THE TRANSIT PEPTIDE OF CFOII IS
FUNCTIONALLY EQUIVALENT TO A SCC-SPCCIFIC TARGETING SIGNAL 68 1.6.
SEC-MACHINERY IS NOT INVOLVED IN THYLAKOID INTEGRATION OF CFOII WITHIN
INTACT CHLOROPLASTS 72 CONTENTS 2. BIOGENESIS OF THE PSAF PROTEIN 74
2.1. MEMBRANE ASSOCIATION AND INTEGRATION OF PSAF 74 2.1.1. IMPORT OF
PSAF INTO ISOLATED CHLOROPLASTS GENERATES TWO DISTINCT FORMS OF THE POLY
PEPTIDE 74 2.1.2. ONLY THE PROTEASC-RCSISTANT FORM OF PSAF IS ASSEMBLED
INTO THE PHOTOSYSTEM I COMPLEX 77 2.1.3. BOTH PROTEASE-RCSISTANT AND
PROTEASE-SENSITIVE PSAF SPECIES BEHAVE AS INTEGRAL MEMBRANE POLYPEPTIDES
79 2.1.4. COMPLETE INTEGRATION OF THE PARTIALLY PROTEASE-ACCESSIBLE
FRACTION OF PSAF INTO THE MEMBRANE IS DELAYED 82 2.2. MECHANISM OF
THYLAKOID TRANSLOCATION OF PSAF 83 2.2.1. THYLAKOID TRANSLOCATION OF
PSAF DEPENDS UPON BIPARTITE PRESEQUENCES, BUT NOT ON THE HYDROPHOBIC
REGION IN ITS MATURE PORTION 84 2.2.2. THYLAKOID TRANSLOCATION OF PSAF
PROCEEDS VIA AN AZIDE-SENSITIVE MECHANISM 87 2.2.3. SENSITIVITY OF
THYLAKOID TRANSLOCATION TO AZIDE IS MEDIATED BY SIGNALS PRESENT IN THE
PSAF TRANSIT PEPTID 88 2.2.4. ANALYSIS OF TARGETING INFORMATION PRESENT
IN THE PSAF TRANSIT PEPTIDE IN IN THYLAKOIDO IMPORT EXPERIMENTS 89
2.2.5. THE PATTERN OF AZIDE INHIBITION INDICATES TWO DISTINCT STEPS IN
THE THYLAKOID TRANSLOCATION OF PSAF 91 2.2.6. DELETION OF THE
HYDROPHOBIC DOMAIN IN THE MATURE PORTION OF PSAF ELIMINATES THE SECOND
STEP OF ITS THYLAKOID TRANSLOCATION 93 2.2.7. ANALYSIS OF TRANSLOCATION
PATHWAY OF PSAF IN COMPETITION EXPERIMENTS 94 2.2.8. SIMULTANEOUS
SATURATION OF THE APH-DEPENDENT AND THE SEC-DEPENDENT PATHWAYS BLOCKS
MEMBRANE INTEGRATION OF PSAF, BUT NOT ITS TARGETING TO THYLAKOIDS 96
2.2.9. COMPETITION ANALYSIS OF PSAF/PC THYLAKOID IRANSLOCATION PRODUCES
AMBIGUOUS RESULTS 97 2.2.10. OVEREXPRESSED PSAF OR PSAF/PC PRECURSORS DO
NOT AFFECT TRANSPORT OF MOST THYLAKOID PROTEINS 98 2.2.11. COMPETITION
EXPERIMENTS SUGGEST IMPLICATION OF COMMON COMPONENTS IN THE
TRANSLOCATION PATHWAYS OF PSAF AND THE RICSKE PROTEIN 102 CONTENTS 3.
THYLAKOID TRANSPORT AND MEMBRANE INTEGRATION OF THE RIESKE FC-S PROTEIN
105 3.1. THE TRANSIT PEPTIDE OF THE RIESKE PROTEIN 106 3.2. MECHANISM OF
THYLAKOID TRANSLOCATION OF THE RIESKE PROTEIN 110 3.2.1. BOTH NIGERICIN
AND AZIDE BLOCK THE THYLAKOID TRANSLOCATION OF THE RIESKE PROTEIN 110
3.2.2. RIESKE PROTEIN UTILIZES THE APH-DEPENDENT PATHWAY FOR INTEGRATION
INTO THE THYLAKOID MEMBRANE BUT IS AFFECTED BY SEC-SPECIFIC COMPETITOR
AS WELL -. 110 3.2.3. RIESKE PROTEIN INTERACTS WITH SLROMAL FACTORS AND
MIGHT SHARE COMMON COMPONENTS WITH THE TRANSPORT INTERMEDIATES OF THE
33-KDA PROTEIN... 111 3.2.4. STROMAL SECA HOMOLOGUE IS INVOLVED IN
THYLAKOID TRANSLOCATION OF THE 33 KDA, BUT NOT OF THE RIESKE PROTEIN 113
3.2.5. INHIBITION OF THYLAKOID TRANSLOCATION OF THE RIESKE PROTEIN IN
THE PRESENCE OF AZIDE MAY RESULT FROM DESTABILIZATION OF ITS INTERACTION
WITH MOLECULAR CHAPERONCS 115 3.3. INFLUENCE OF THE PRESENCE OF TWO
DIFFERENT THYLAKOID TARGETING SIGNALS ON THE MECHANISM OF THYLAKOID
TRANSLOCATION OF THE RIESKE PROTEIN 118 3.3.1. THYLAKOID MEMBRANE
INTEGRATION OF THE PC/RIESKE PROTEIN 118 3.3.1.1. ASSEMBLY OF THE NEWLY
IMPORTED RIESKE AND PC/RIESKE POLYPEPTIDCS INTO THE CYTOCHROMC {^COMPLEX
OF THYLAKOIDS 119 A. BOTH THE INTERMEDIATE AND THE MATURE FORMS OF THE
PC/RIESKE PROTEIN CAN BE FOUND ASSEMBLED IN THE CYTOCHROME BF/F COMPLEX
..119 B. INTEGRATION OF THE AUTHENTIC RIESKE PROTEIN INTO THE CYTOCHROME
COMPLEX PROCEEDS WITH AN EXCEPTIONALLY HIGH EFFICIENCY 12 0 3.3.1.2.
MECHANISM OF THYLAKOID INTEGRATION OF THE PC/RICSKC PROTEIN 122 A.
INFLUENCE OF NIGERICIN AND AZIDE ON THYLAKOID INTEGRATION OF PC/RIESKE
122 B. COMPETITION EXPERIMENTS DEMONSTRATE THAT THE INTERMEDIATE AND THE
MATURE FORMS OF PC/RIESKE PROTEIN IN THYLAKOIDS RESULT FROM
TRANSLOCATION BY DIFFERENT MECHANISMS 123 3.3.2. THYLAKOID TRANSPORT-OF
THE 23/RICSKE PROTEIN 125 3.3.2.1. THYLAKOID INTEGRATION OF 23/RICSKE
LEADS TO AN INVERSION OF THE CONTENTS MEMBRANE TOPOLOGY OF THE RIESKE
PROTEIN 126 3.3.2.2. ONLY THE CORRECTLY ORIENTED FORM OF THE RIESKE
PROTEIN CAN BE ASSEMBLED IN THE CYTOCHROME/^// COMPLEX OF THYLAKOIDS 127
3.3.2.3. THE INVERTED FORM OF THE 23/RIESKE POLYPEPTIDE DOES NOT
UNDERGO FURTHER TRANSLOCATION ACROSS THE THYLAKOID MEMBRANE 128 3.3.2.4.
MECHANISM OF THYLAKOID INTEGRATION OF THE 23/RIESKE PROTEIN 129 3.4.
ACQUISITION OF THE FE-S CENTER OF THE RIESKE PROTEIN IS REQUIRED FOR ITS
TRANSPORT ACROSS THE THYLAKOID MEMBRANE 134 3.4.1. MUTATIONS WITHIN THE
FC-S CLUSTER BINDING SITE OF THE RIESKE PROTEIN PREVENT ITS THYLAKOID
TRANSLOCATION 134 3.4.2. IRON-SPECIFIC CHELATOR BLOCKS THE THYLAKOID
TRANSPORT OF THE RIESKE PROTEIN 136 3.5. THE MODE OF MEMBRANE
ASSOCIATION OF THE RIESKE PROTEIN AND TOPOLOGICAL ANALYSIS OF SUBUNITS
OF THE CYTOCHROME B ( JF COMPLEX 139 3.5.1. THE PC/RIESKE POLYPEPTIDE
HAS A TRANSMEMBRANC TOPOLOGY UPON INSERTION INTO THE THYLAKOID MEMBRANE
139 3.5.2. THE N-TERMINAL SEGMENT OF THE RIESKE PROTEIN IS EXPOSED ON
THE STROMAL SURFACE OF THYLAKOID MEMBRANE 140 3.5.3. MEMBRANE
ASSOCIATION OF SUBUNITS OF THE CYTOCHROME B ( JF COMPLEX... 142 3.5.4.
MEMBRANE STABILITY OF THE RIESKE PROTEIN IS INCREASED IN THE PRESENCE OF
SUCROSE IN THE MEDIUM 143 3.5.5. UNEXPECTEDLY LOW MEMBRANE STABILITY OF
CYTOCHROME J 6 145 3.6. ANALYSIS OF STROMAL FACTORS INVOLVED IN THE
THYLAKOID TRANSLOCATION OF THE RIESKE PROTEIN 147 3.6.1. GENERAL
CHARACTERIZATION OF INTERACTION OF THE RIESKE PROTEIN WITH STROMAL
FACTORS 147 3.6.2. ISOLATION OF PROTEINS INVOLVED IN INTERACTIONS WITH
THE RIESKE PROTEIN IN STROMA BY METAL-CHEIATE AFFINTY CHROMATOGRAPHY 153
3.6.3. RUBISCO ACTIVASE AS A CHAPCRONE FACTOR OF STROMA INVOLVED IN
INTERACTION WITH TRANSPORT INTERMEDIATES OF THYLAKOID PROTEINS 155
3.6.4. SPECIAL FEATURES OF INTERACTION OF THE RIESKE PROTEIN WITH CPN60
AND RUBISCO ACTIVASE 159 CONTENTS 3.6.5. CLPC, THE STROMAL HSPLOO,
INTERACTS WITH TRANSPORT INTERMEDIATES OF THE RIESKE PROTEIN 162 3.6.6.
INTERACTION WITH MOLECULAR CHAPCRONES DURING THYLAKOID TRANSLOCATION OF
THE RIESKE PROTEIN 163 DISCUSSION 166 1. THYLAKOID IRANSLOCATION OF THE
RIESKE PROTEIN 166 2. STRUCTURAL ASPECTS OF BIOGENESIS OF THE RIESKE
FC-S PROTEIN 172 3. THYLAKOID TRANSPORT AND INTEGRATION OF THE PSAF
PROTEIN 176 4. THYLAKOID INTEGRATION OF CFOI 1 179 5. FACTORS WHICH CAN
INFLUENCE THE SELECTION OF THE TRANSLOCATION PATHWAY OF A PROTEIN 180 6.
IMPLICATIONS FOR DISTINCT THYLAKOID TRANSLOCATION PATHWAYS 182 SUMMARY
187 REFERENCES 191 LIST OF PUBLICATIONS 210 DNNKSNGUNG 211 CURRICULUM
VITAE 214
|
| any_adam_object | 1 |
| author | Karnauchov, Ivan 1970- |
| author_GND | (DE-588)12098346X |
| author_facet | Karnauchov, Ivan 1970- |
| author_role | aut |
| author_sort | Karnauchov, Ivan 1970- |
| author_variant | i k ik |
| building | Verbundindex |
| bvnumber | BV012455121 |
| ctrlnum | (OCoLC)246436369 (DE-599)BVBBV012455121 |
| format | Thesis Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01630nam a2200397 c 4500</leader><controlfield tag="001">BV012455121</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20030314 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">990315s1998 ad|| m||| 00||| gerod</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)246436369</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV012455121</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakddb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">ger</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-19</subfield><subfield code="a">DE-29T</subfield><subfield code="a">DE-355</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-188</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Karnauchov, Ivan</subfield><subfield code="d">1970-</subfield><subfield code="e">Verfasser</subfield><subfield code="0">(DE-588)12098346X</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins</subfield><subfield code="c">von Ivan Karnauchov</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">1998</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">214 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="502" ind1=" " ind2=" "><subfield code="a">München, Univ., Diss., 1998</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Membranproteine</subfield><subfield code="0">(DE-588)4130026-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Protein-Sortierung</subfield><subfield code="0">(DE-588)4533502-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Chloroplast</subfield><subfield code="0">(DE-588)4147821-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Thylakoidmembran</subfield><subfield code="0">(DE-588)4185376-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4113937-9</subfield><subfield code="a">Hochschulschrift</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Chloroplast</subfield><subfield code="0">(DE-588)4147821-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Thylakoidmembran</subfield><subfield code="0">(DE-588)4185376-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Membranproteine</subfield><subfield code="0">(DE-588)4130026-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Protein-Sortierung</subfield><subfield code="0">(DE-588)4533502-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">GBV Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008451658&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="940" ind1="1" ind2=" "><subfield code="n">oe</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-008451658</subfield></datafield></record></collection> |
| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV012455121 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T18:27:52Z |
| institution | BVB |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-008451658 |
| oclc_num | 246436369 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-29T DE-355 DE-BY-UBR DE-12 DE-11 DE-188 |
| owner_facet | DE-19 DE-BY-UBM DE-29T DE-355 DE-BY-UBR DE-12 DE-11 DE-188 |
| physical | 214 S. Ill., graph. Darst. |
| publishDate | 1998 |
| publishDateSearch | 1998 |
| publishDateSort | 1998 |
| record_format | marc |
| spelling | Karnauchov, Ivan 1970- Verfasser (DE-588)12098346X aut Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins von Ivan Karnauchov 1998 214 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 1998 Membranproteine (DE-588)4130026-9 gnd rswk-swf Protein-Sortierung (DE-588)4533502-3 gnd rswk-swf Chloroplast (DE-588)4147821-6 gnd rswk-swf Thylakoidmembran (DE-588)4185376-3 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Chloroplast (DE-588)4147821-6 s Thylakoidmembran (DE-588)4185376-3 s Membranproteine (DE-588)4130026-9 s Protein-Sortierung (DE-588)4533502-3 s DE-604 GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008451658&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Karnauchov, Ivan 1970- Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins Membranproteine (DE-588)4130026-9 gnd Protein-Sortierung (DE-588)4533502-3 gnd Chloroplast (DE-588)4147821-6 gnd Thylakoidmembran (DE-588)4185376-3 gnd |
| subject_GND | (DE-588)4130026-9 (DE-588)4533502-3 (DE-588)4147821-6 (DE-588)4185376-3 (DE-588)4113937-9 |
| title | Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins |
| title_auth | Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins |
| title_exact_search | Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins |
| title_full | Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins von Ivan Karnauchov |
| title_fullStr | Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins von Ivan Karnauchov |
| title_full_unstemmed | Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins von Ivan Karnauchov |
| title_short | Mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins |
| title_sort | mechanisms of intrachloroplast sorting of integral thylakoid membrane proteins |
| topic | Membranproteine (DE-588)4130026-9 gnd Protein-Sortierung (DE-588)4533502-3 gnd Chloroplast (DE-588)4147821-6 gnd Thylakoidmembran (DE-588)4185376-3 gnd |
| topic_facet | Membranproteine Protein-Sortierung Chloroplast Thylakoidmembran Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008451658&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT karnauchovivan mechanismsofintrachloroplastsortingofintegralthylakoidmembraneproteins |