Techniques in microbial ecology:
Microbial ecology is one of the fastest growing fields of microbiology. This practical volume is the bench and field scientist's guide to well-established and reliable techniques for investigating microbial communities. Both for biologists just entering the field and for experienced researchers...
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York [u.a.]
Oxford Univ. Press
1998
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Zusammenfassung: | Microbial ecology is one of the fastest growing fields of microbiology. This practical volume is the bench and field scientist's guide to well-established and reliable techniques for investigating microbial communities. Both for biologists just entering the field and for experienced researchers wishing to explore new areas, this book provides the theoretical background, detailed protocols, and tips from experts essential for working in this field. Chapters on bacteria with interesting metabolic traits are augmented with chapters on molecular techniques, lipid analysis, and appropriate sampling techniques. The final section includes up-to-date information on biofilm development and study, the science and practice of bioremediation, modeling of biological systems (including the most useful statistical parameters), and the study of phylogenetics. |
| Beschreibung: | XII, 468 S. Ill., graph. Darst. |
| ISBN: | 0195092236 9780195092233 |
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| 245 | 1 | 0 | |a Techniques in microbial ecology |c ed. by Robert S. Burlage ... |
| 264 | 1 | |a New York [u.a.] |b Oxford Univ. Press |c 1998 | |
| 300 | |a XII, 468 S. |b Ill., graph. Darst. | ||
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| 520 | 3 | |a Microbial ecology is one of the fastest growing fields of microbiology. This practical volume is the bench and field scientist's guide to well-established and reliable techniques for investigating microbial communities. Both for biologists just entering the field and for experienced researchers wishing to explore new areas, this book provides the theoretical background, detailed protocols, and tips from experts essential for working in this field. Chapters on bacteria with interesting metabolic traits are augmented with chapters on molecular techniques, lipid analysis, and appropriate sampling techniques. The final section includes up-to-date information on biofilm development and study, the science and practice of bioremediation, modeling of biological systems (including the most useful statistical parameters), and the study of phylogenetics. | |
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| 650 | 7 | |a Biodegradatie |2 gtt | |
| 650 | 7 | |a Biotechnologie |2 gtt | |
| 650 | 7 | |a Microbiologie - Manuels de laboratoire |2 ram | |
| 650 | 7 | |a Microbiologie |2 gtt | |
| 650 | 7 | |a Milieubiologie |2 gtt | |
| 650 | 7 | |a Milieutechniek |2 gtt | |
| 650 | 7 | |a Onderzoeksmethoden |2 gtt | |
| 650 | 7 | |a Écologie microbienne - Manuels de laboratoire |2 ram | |
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Datensatz im Suchindex
| _version_ | 1804127054164131840 |
|---|---|
| adam_text | Contents
Contributors
xi
PART I ENVIRONMENTAL MICROBIOLOGY
1
Microbially Mediated Nitrogen Cycling
3
Hans W. Paerl
Biologically Available Forms of Nitrogen: Analyses
5
Nitrogen Transformations: Characterizing Relevant Microbiota and their
Activities
8
References
27
2
Microbes of the Sulfur Cycle
31
Don P. Kelly and Ann P. Wood
Techniques
32
Types of Bacteria
34
Stock Solutions and Culture Media
35
Assays of Substrates and Intermediates
43
Enzyme Assays
52
References
56
3
Isolation, Cultivation and Characterization of Iron- and Manganese-
Reducing Bacteria
58
Joel
Kostka
and Kenneth H. Neabon
Media Preparation
60
Preparation of the Oxidized Metals
63
Monitoring
Metal Reduction in Culture
68
viii CONTENTS
Enrichment, Estimation of Abundance, and Isolation of Metal-Reducing
Bacteria
70
Growth in the Laboratory: General Considerations
74
Conclusion
75
References
75
4
The Aerobic, Hydrogen-Oxidizing
(Knallgas)
Bacteria
79
Michel Aragno
Hydrogen in Natural Environments
87
Culture Methods
89
Identification
99
References
106
5
Methanogens
113
Stephen H.
Zinder
Cultural Methods for Studying
Methanogens
116
Methods to Quantify Methanogenic Activity
124
Microscopic Methods to Study
Methanogens
in Natural Habitats
127
References
130
6
Ecology of Methylotrophic Bacteria
137
Richard S. Hanson
Measurements of Methane Oxidation in Environmental Samples
141
Isolation of Pure Cultures of Methylotrophic Bacteria from Natural Samples and
their Characterization
145
Detection of Methylotrophic Bacteria in Environmental Samples Using Gene
Probes and Phylogenetic Probes
151
Ecologie
Studies that Have Employed Gene and Phylogenetic Probes
155
Growth Media
158
References
160
7
Terrestrial Fungal Communities
163
Alan D. M.
Rayner
and
Lynne
Boddy
Locating and Identifying Fungi
164
Isolation of Spores
—
Non-Species-Specific Techniques
172
Isolation of Culturable Fungi
—
From Mycelia
175
Molecular Detection of Unculturable Fungi
176
Identifying Cultures and Spores
177
Interpreting Distribution Patterns from an Organizational Viewpoint
181
Snapshots of Metabolic Activity
194
References
198
8
Rapid Techniques for the Identification of Free-Living Protozoa
203
G. F.
Esteban,
K. J.
Clarke and
B. J. Finlay
Identifying Ciliated Protozoa with the Silver Carbonate Impregnation
Technique
204
Rapid Specimen Preparation Methods for eectron Microscopy
207
References
216
ľJ
CONTENTS ix
9
Methods for Enumeration and Characterization of Bacteriophages from
Environmental Samples
218
Robert V Miller
Laboratory Techniques
219
Genetic Methods: Transductional Analysis
225
Environmental Methods
227
References
233
PART II ENVIRONMENTAL TECHNIQUES
10
Sampling And Enumeration Techniques
239
J. K. Fredrickson and D. L. Balkwill
Methods for Sampling Environmental Media
240
Total Counting and Biomass Methods
244
Examples of Media for Plating Soil and Subsurface Sediment Samples
251
References
252
11
Signature
Lipid
Biomarker
Analysis
255
David C. White and David B.
Ringelberg
Lipid
Extraction
255
Silicic Acid Column Chromatography
258
Preparation of Fatty Acid Methyl Esters from Esterified Lipids
259
Separation, Quantification, and Identification of Organic Compounds
261
Preparation of
Poly-ß-Hydroxyalkanoates
from Glycolipids
263
Preparation of Sterols from Esterified Lipids
265
Preparation of Lipopolysaccharide Hydroxy Fatty Acids
266
Preparation of Dimethyl Disulfide Adducts of Monounsaturated Fame
268
Preparation of Archaebacterial or Thermodesulfobacterium commune Ether
Lipids for Supercritical Fluid Chromatography
269
References
271
12
Isolation of Nucleic Acids From Environmental Samples
273
Andrew
Ogram
Factors Affecting Recovery of Nucleic Acids
274
Cell Fractionation Versus Direct Lysis
280
Methods
281
References
287
13
Molecular Techniques
289
Robert S. Burlage
Gene Cloning
—
an Overview
290
DNA
Transfer
306
Hybridization Analysis
312
Polymerase Chain Reaction
322
Restriction Fragment Length Polymorphism
328
DNA
Sequencing
330
References
331
x
CONTENTS
PARTIU
SPECIAL
TOPICS
14
Laboratory
Studies
Of Bacterial
Biofilms
337
M. W.
Mittelman
Test Systems
338
Quantitative Recovery of Cells from Surfaces
346
Evaluation of Bacterial Numbers
347
Determination of
Biofilm Biomass
Constituents
349
Determination of
Biofilm
Metabolic Activity
350
Electron Microscopic Examination of
Biofilm
Architecture
351
References
352
Bibliography
352
15
Theoretical and Applied Aspects of Bioremediation: The Influence of
Microbiological Processes on Organic Contaminant Compounds in
Field Sites
354
Eugene L. Madsen
Conceptual Overview of Bioremediation
355
Techniques of Bioremediation and
Biodegradation 363
Concluding Remarks
394
References
394
16
Mathematical Modeling and Statistical Analysis
408
J.
/.
Prosser
Mathematical Models
408
Statistical Analysis
419
Conclusions
436
References
436
1 ?
Numerical Classification Methods
438
Estelle
Russek-Cohen and Rita R. Colwell
Data Quality
439
Tree Construction
442
Evaluation and Validation of Results
448
Identification
449
Ordination Methods
452
References
452
Index
455
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| language | English |
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| spelling | Techniques in microbial ecology ed. by Robert S. Burlage ... New York [u.a.] Oxford Univ. Press 1998 XII, 468 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Microbial ecology is one of the fastest growing fields of microbiology. This practical volume is the bench and field scientist's guide to well-established and reliable techniques for investigating microbial communities. Both for biologists just entering the field and for experienced researchers wishing to explore new areas, this book provides the theoretical background, detailed protocols, and tips from experts essential for working in this field. Chapters on bacteria with interesting metabolic traits are augmented with chapters on molecular techniques, lipid analysis, and appropriate sampling techniques. The final section includes up-to-date information on biofilm development and study, the science and practice of bioremediation, modeling of biological systems (including the most useful statistical parameters), and the study of phylogenetics. Biochemie gtt Biodegradatie gtt Biotechnologie gtt Microbiologie - Manuels de laboratoire ram Microbiologie gtt Milieubiologie gtt Milieutechniek gtt Onderzoeksmethoden gtt Écologie microbienne - Manuels de laboratoire ram Microbial ecology Laboratory manuals Molecular microbiology Laboratory manuals Ökologie (DE-588)4043207-5 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Mikrobiologie (DE-588)4316357-9 gnd rswk-swf Ökologie (DE-588)4043207-5 s Mikrobiologie (DE-588)4316357-9 s Methode (DE-588)4038971-6 s DE-604 Burlage, Robert S. Sonstige oth Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008425535&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Techniques in microbial ecology Biochemie gtt Biodegradatie gtt Biotechnologie gtt Microbiologie - Manuels de laboratoire ram Microbiologie gtt Milieubiologie gtt Milieutechniek gtt Onderzoeksmethoden gtt Écologie microbienne - Manuels de laboratoire ram Microbial ecology Laboratory manuals Molecular microbiology Laboratory manuals Ökologie (DE-588)4043207-5 gnd Methode (DE-588)4038971-6 gnd Mikrobiologie (DE-588)4316357-9 gnd |
| subject_GND | (DE-588)4043207-5 (DE-588)4038971-6 (DE-588)4316357-9 |
| title | Techniques in microbial ecology |
| title_auth | Techniques in microbial ecology |
| title_exact_search | Techniques in microbial ecology |
| title_full | Techniques in microbial ecology ed. by Robert S. Burlage ... |
| title_fullStr | Techniques in microbial ecology ed. by Robert S. Burlage ... |
| title_full_unstemmed | Techniques in microbial ecology ed. by Robert S. Burlage ... |
| title_short | Techniques in microbial ecology |
| title_sort | techniques in microbial ecology |
| topic | Biochemie gtt Biodegradatie gtt Biotechnologie gtt Microbiologie - Manuels de laboratoire ram Microbiologie gtt Milieubiologie gtt Milieutechniek gtt Onderzoeksmethoden gtt Écologie microbienne - Manuels de laboratoire ram Microbial ecology Laboratory manuals Molecular microbiology Laboratory manuals Ökologie (DE-588)4043207-5 gnd Methode (DE-588)4038971-6 gnd Mikrobiologie (DE-588)4316357-9 gnd |
| topic_facet | Biochemie Biodegradatie Biotechnologie Microbiologie - Manuels de laboratoire Microbiologie Milieubiologie Milieutechniek Onderzoeksmethoden Écologie microbienne - Manuels de laboratoire Microbial ecology Laboratory manuals Molecular microbiology Laboratory manuals Ökologie Methode Mikrobiologie |
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