2-D proteome analysis protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
1999
|
| Schriftenreihe: | Methods in molecular biology
112 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVII, 601 S. Ill., graph. Darst. |
| ISBN: | 0896035247 |
Internformat
MARC
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| 245 | 1 | 0 | |a 2-D proteome analysis protocols |c ed. by Andrew J. Link |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 1999 | |
| 300 | |a XVII, 601 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 112 | |
| 650 | 7 | |a Elektroforese |2 gtt | |
| 650 | 7 | |a Genexpressie |2 gtt | |
| 650 | 7 | |a Karakterisering (algemeen) |2 gtt | |
| 650 | 7 | |a Proteomen |2 gtt | |
| 650 | 4 | |a Electrophoresis, Gel, Two-Dimensional |x methods | |
| 650 | 4 | |a Gel electrophoresis | |
| 650 | 4 | |a Gene Expression | |
| 650 | 4 | |a Gene expression |x Research |x Methodology | |
| 650 | 4 | |a Proteins |x Analysis | |
| 650 | 4 | |a Proteins |x analysis | |
| 650 | 4 | |a Sequence Analysis |x methods | |
| 650 | 0 | 7 | |a Genexpression |0 (DE-588)4020136-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Proteine |0 (DE-588)4076388-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Zweidimensionale Elektrophorese |0 (DE-588)4221393-9 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1804127022209826816 |
|---|---|
| adam_text | Contents
Preface v
Contributors xiii
1 2 D Protein Gel Electrophoresis: An Overview
Jenny Fichmann and Reiner Westermeier 1
2 Solubilization of Proteins in 2 D Electrophoresis: An Outline
Thierry Rabilloud 9
3 Preparation of Escherichia coli Samples for 2 D Gel Analysis
Ruth A. VanBogelen and Frederick C. Neidhardt 21
4 Preparing 2 D Protein Extracts from Yeast
David M. Schieltz 31
5 2 D Protein Extracts from Drosophila melanogaster
Christer Ericsson 35
6 Preparing 2 D Protein Extracts from Caenorhabditis elegans
Robert Zwilling 43
7 Eukaryotic Cell Labeling and Preparation for 2 D
NickBizios 49
8 Differential Detergent Fractionation of Eukaryotic Cells: Analysis by
Two Dimensional Gel Electrophoresis
Melinda L. Ramsby and Gregorys. Makowski 53
9 Fractionated Extraction of Total Tissue Proteins from Mouse and
Human for 2 D Electrophoresis
Joachim Klose 67
10 Preparation and Solubilization of Body Fluids for 2 D
Jean Charles Sanchez and Denis F. Hochstrasser 87
11 2 D Electrophoresis of Plant Proteins
Akira Tsugita and Masaharu Kamo 95
12 Quantifying Protein in 2 D PAGE Solubilization Buffers
Louis S. Ramagli 99
13 Measuring the Radioactivity of 2 D Protein Extracts
Andrew J. Link and Nick Bizios 105
14 Advantages of Carrier Ampholyte IEF
Mary F. Lopez 109
vii
viii Contents
15 2 D Electrophoresis Using Carrier Ampholytes in the First
Dimension (IEF)
Mary F. Lopez / / 1
16 Nonequilibrium pH Gel Electrophoresis (NEPHGE)
Mary F. Lopez 129
17 High Resolution, 2 D Protein Electrophoresis Using Nondedicated
Equipment
Marion Sarmiento 133
18 Large Gel 2 D Electrophoresis
Joachim Klose 747
19 Advantages of Immobilized pH Gradients
Jenny Fichmann 173
20 Casting Immobilized pH Gradients (IPGs)
Elisabetta Gianazza 175
21 Analytical IPG Dalt
Angelika Gorg and Walter Weiss 189
22 IPG Dalt of Very Alkaline Proteins
Angelika Gorg 197
23 Running Preparative Carrier Ampholyte and Immobilized pH
Gradient IEF Gels for 2 D
Neil M. Matsui, Diana M. Smith Beckerman, Jenny Fichmann,
and Lois B. Epstein 211
24 In Gel Sample Rehydration of Immobilized pH Gradient
Jean Charles Sanchez, Denis Hochstrasser,
and Thierry Rabilloud 221
24a High Resolution, IPG Based, Mini Two Dimensional
Gel Electrophoresis
Jean Charles Sanchez and Denis F. Hochstrasser 227
25 Horizontal SDS PAGE for IPG Dalt
Angelika Gorg and Walter Weiss 235
26 Casting and Running Vertical Slab Gel Electrophoresis
for2D PAGE
Bradley J. Walsh and Benjamin R. Herbert 245
27 Nonreducing 2 D Polyacrylamide Gel Electrophoresis
Hong Ji and Richard J. Simpson 255
28 2 D Diagonal Gel Electrophoresis
Joan Goverman 265
Contents ix
29 2 D Phosphopeptide Mapping
Hikaru Nagahara, Robert R. Latek, Sergei A. Ezhevsky,
and Steven F. Dowdy 271
30 Internal Standards for 2 D
Andrew J. Link 281
31 Autoradiography of 2 D Gels
Andrew J. Link 285
32 Double Label Analysis
Kelvin H. Lee and Michael G. Harrington 291
33 Silver Staining of 2 D Electrophoresis Gels
Thierry Rabilloud 297
34 Staining of Preparative 2 D Gels: Coomassie Blue and
Imidazole Zinc Negative Staining
Neil M. Matsui, Diana M. Smith Becker man,
and Lois B. Epstein 307
35 Electroblotting of Proteins from 2 D Polyacrylamide Gels
Michael J. Dunn 313
36 Detection of Total Proteins on Western Blots of
2 D Polyacrylamide Gels
Michael J. Dunn 319
37 Protein Detection Using Reversible Metal Chelate Stains
Wayne F. Patton, Mark J. Lim, and David Shepro 331
38 Gylcoprotein Detection of 2 D Separated Proteins
Nicolle H. Packer, Malcolm S. Ball, and Peter L. Devine 341
39 Image Acquisition in 2 D Electrophoresis
Wayne F. Patton, Mark J. Lim, and David Shepro 353
40 Computer Analysis of 2 D Images
Ron D. Appel and Denis F. Hochstrasser 363
41 2 D Databases on the World Wide Web
Ron D. Appel, Amos Bairoch, and Denis F. Hochstrasser 383
42 Comparing 2 D Electrophoresis Gels Across Internet Databases
Peter F. Lemkin 393
43 Constructing a 2 D Database for the World Wide Web
Ron D. Appel, Christine Hoogland, Amos Bairoch,
and Denis F. Hochstrasser 411
44 Absolute Quantitation of 2 D Protein Spots
Steven P. Gygi and Ruedi Aebersold 417
x Contents
45 Generating a Bacterial Genome Inventory: Identifying 2 D Spots
by Comigrating Products of the Genome on 2 D Gels
Ruth A. VanBogelen 423
46 Immunoaffinity Identification of 2 DE Separated Proteins
Barbara Magi, Luca Bini, Barbara Marzocchi,
Sabrina Liberatori, Roberto Raggiaschi,
and Vitaliano Pallini 431
47 2 DE Spot Amino Acid Analysis with 9 Fluroenylmethyl
Chloroformate
Marc R. Wilkins, Jun X. Yan, and Andrew A. Gooley 445
48 N Terminal Amino Acid Sequencing of 2 DE Spots
Masaharu Kamo and Akira Tsugita 461
49 Characterizing Proteins from 2 DE Gels by Internal
Sequence Analysis of Peptide Fragments:
Strategies for Microsample Handling
Hediye Erdjument Bromage, Mary Lui, Lynne Lacomis,
and Paul Tempst 467
50 Obtaining Molecular Weights of Proteins and Their Cleavage
Products by Directly Combining Gel Electrophoresis
with Mass Spectrometry
Rachel R. Ogorzalek Loo, Joseph A. Loo,
and Philip C. Andrews 473
51 Identification of Proteins by Matrix Assisted Laser
Desorption/lonization Mass Spectrometry Using Peptide
and Fragment Ion Masses
Paul L. Courchesne and Scott D. Patterson 487
52 Sample Preparation Methods for Mass Spectrometric Peptide
Mapping Directly from 2 DE Gels
Ole Norregaard Jensen, Matthias Wilm, Andre] Shevchenko,
and Matthias Mann 513
53 Protein Identification and Analysis Tools in the ExPASy Server
Marc R. Wilkins, Elisabeth Gasteiger, Amos Bairoch,
Jean Charles Sanchez, Keith L. Williams, Ron D. Appel,
and Denis F. Hochstrasser 531
54 Automated Protein Identification Using Microcolumn Liquid
Chromatography Tandem Mass Spectrometry
John R. Yates, III, Edwin Carmack, Lara Hays, Andrew J. Link,
and Jimmy K. Eng 553
Contents xi
55 Peptide Sequencing of 2 DE Gel Isolated Proteins by
Nanoelectrospray Tandem Mass Spectrometry
Ole Nerregaard Jensen, Matthias Wilm, Andrej Shevchenko,
and Matthias Mann 571
Index 589
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| indexdate | 2024-07-09T18:26:44Z |
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| isbn | 0896035247 |
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| spelling | 2-D proteome analysis protocols ed. by Andrew J. Link Totowa, NJ Humana Press 1999 XVII, 601 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 112 Elektroforese gtt Genexpressie gtt Karakterisering (algemeen) gtt Proteomen gtt Electrophoresis, Gel, Two-Dimensional methods Gel electrophoresis Gene Expression Gene expression Research Methodology Proteins Analysis Proteins analysis Sequence Analysis methods Genexpression (DE-588)4020136-3 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Genexpression (DE-588)4020136-3 s Zweidimensionale Elektrophorese (DE-588)4221393-9 s DE-604 Link, Andrew J. Sonstige oth Methods in molecular biology 112 (DE-604)BV035362695 112 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008403920&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | 2-D proteome analysis protocols Methods in molecular biology Elektroforese gtt Genexpressie gtt Karakterisering (algemeen) gtt Proteomen gtt Electrophoresis, Gel, Two-Dimensional methods Gel electrophoresis Gene Expression Gene expression Research Methodology Proteins Analysis Proteins analysis Sequence Analysis methods Genexpression (DE-588)4020136-3 gnd Proteine (DE-588)4076388-2 gnd Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd |
| subject_GND | (DE-588)4020136-3 (DE-588)4076388-2 (DE-588)4221393-9 (DE-588)4143413-4 |
| title | 2-D proteome analysis protocols |
| title_auth | 2-D proteome analysis protocols |
| title_exact_search | 2-D proteome analysis protocols |
| title_full | 2-D proteome analysis protocols ed. by Andrew J. Link |
| title_fullStr | 2-D proteome analysis protocols ed. by Andrew J. Link |
| title_full_unstemmed | 2-D proteome analysis protocols ed. by Andrew J. Link |
| title_short | 2-D proteome analysis protocols |
| title_sort | 2 d proteome analysis protocols |
| topic | Elektroforese gtt Genexpressie gtt Karakterisering (algemeen) gtt Proteomen gtt Electrophoresis, Gel, Two-Dimensional methods Gel electrophoresis Gene Expression Gene expression Research Methodology Proteins Analysis Proteins analysis Sequence Analysis methods Genexpression (DE-588)4020136-3 gnd Proteine (DE-588)4076388-2 gnd Zweidimensionale Elektrophorese (DE-588)4221393-9 gnd |
| topic_facet | Elektroforese Genexpressie Karakterisering (algemeen) Proteomen Electrophoresis, Gel, Two-Dimensional methods Gel electrophoresis Gene Expression Gene expression Research Methodology Proteins Analysis Proteins analysis Sequence Analysis methods Genexpression Proteine Zweidimensionale Elektrophorese Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008403920&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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