Manual of immunological methods:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Boca Raton [u.a.]
CRC Press
1999
|
| Schriftenreihe: | Pharmacology and toxicology
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 141 S. Ill., graph. Darst. |
| ISBN: | 084938558X |
Internformat
MARC
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| 245 | 1 | 0 | |a Manual of immunological methods |c Pauline Brousseau ... |
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| 300 | |a 141 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 490 | 0 | |a Pharmacology and toxicology | |
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| 650 | 4 | |a Immunologie - Technique - Guides, manuels, etc | |
| 650 | 4 | |a Inmunología - Técnica - Manuales | |
| 650 | 4 | |a Animals, Laboratory | |
| 650 | 4 | |a Immunoassay |x Handbooks, manuals, etc | |
| 650 | 4 | |a Immunologic Techniques | |
| 650 | 4 | |a Immunology |x Technique |x Handbooks, manuals, etc | |
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Datensatz im Suchindex
| _version_ | 1804126984176926721 |
|---|---|
| adam_text | Table of Contents
Preface xiii
List of Abreviations xvii
List of Species Included in the Handbook xviii
Chapter 1
Identification, Anesthesia, and Euthanasia
1.1 Introduction 1
1.2 Identification of Laboratory Rodents by Ear Notching 1
1.2.1 Reagent 1
1.2.2 Materials and Equipment 1
1.2.3 Procedure 1
1.3 Identification of Fish by Fin Clipping 2
1.3.1 Reagent 2
1.3.2 Materials and Equipment 2
1.3.3 Procedure 3
1.4 Anesthesia of Fish and Rodents 3
1.5 Euthanasia of Rodents 3
1.5.1 Materials and Equipment 3
1.5.2 Procedure 3
1.6 Euthanasia of Fish 4
1.6.1 Reagent 4
1.6.2 Materials and Equipment 4
1.6.3 Procedure 4
1.7 Working Sheet: Weight of Laboratory Animals 5
Suggested Reading 6
Chapter 2
Collection of Peripheral Blood Samples
2.1 Introduction 7
2.2 Reagents 7
2.3 Materials and Equipment 7
2.4 Procedure 8
2.4.1 Cardiac Puncture 8
2.4.2 Orbital Sinus Blood Collection 8
2.4.3 Marginal Ear Vein Blood Collection 8
2.4.4 Caudal Tail Vein Blood Collection 9
2.4.5 Femoral Vein Blood Collection in Monkeys 9
2.4.6 Cephalic or Saphenous Vein Blood Collection in Dogs, Bears 9
2.4.7 Brachial Vein Blood Collection in Birds 10
2.4.8 Jugular Vein Blood Collection 10
Suggested Reading 11
Chapter 3
Removal of Organs
3.1 Introduction 13
3.2 Reagents 13
3.3 Materials and Equipment 13
3.4 Procedure 13
3.4.1 Removal of the Spleen 13
3.4.1.1 Rat or Mouse 13
3.4.1.2 Trout Model 14
3.4.2 Removal of the Thymus 14
3.4.2.1 Rat or Mouse 14
3.4.2.2 Trout 14
3.4.3 Removal of the Lymph Node (Mouse or Rat) 14
3.4.4 Removal of the Head Kidney (Trout) 16
3.4.5 Removal of the Bone Marrow (Mouse and Rat) 16
Suggested Reading 16
Chapter 4
Preparation of Cell Suspensions
4.1 Introduction 17
4.2 Extrusion of Coelomocytes in the Earthworm 17
4.2.1 Reagents 17
4.2.2 Materials and Equipment 17
4.2.3 Procedure 17
4.3 Haemocyte Collection in the Mollusk 18
4.3.1 Reagent 18
4.3.2 Materials and Equipment 18
4.3.3 Procedure 18
4.4 Cell Suspension from Peritoneal Exudate 18
4.4.1 Rat 18
4.4.1.1 Reagents 18
4.4.1.2 Materials and Equipment 19
4.4.1.3 Procedure 19
4.4.2 Birds 19
4.4.2.1 Reagents 19
4.4.2.2 Materials and Equipment 19
4.4.2.3 Procedure 20
4.5 Isolation of White Blood Cells from Peripheral Blood 20
4.5.1 Mammals 20
4.5.1.1 Reagents 20
4.5.1.2 Materials and Equipment 20
4.5.1.3 Procedure 20
4.5.2 Birds 21
4.5.2.1 Reagents 21
4.5.2.2 Materials and Equipment 21
4.5.2.3 Procedure 21
4.5.3 Fish 22
4.5.3.1 Reagents 22
4.5.3.2 Materials and Equipment 22
4.5.3.3 Procedure 22
4.6 Cell Suspensions from Lymphoid Organs 22
4.6.1 Reagents 22
4.6.2 Materials and Equipment 23
4.6.3 Procedure 24
Suggested Reading 25
Chapter 5
Assessment of Cell Viability
5.1 Introduction 27
5.2 Determination of Cell Viability and Concentration by Trypan
Blue Dye Exclusion 27
5.2.1 Reagents 27
5.2.2 Materials and Equipment 27
5.2.3 Procedure 27
5.3 Determination of Cell Viability and Cell Concentration with
Ethidium Bromide and Acridine Orange 28
5.3.1 Reagents 28
5.3.2 Materials and Equipment 29
5.3.3 Procedure 29
5.4 Flow Cytometric Assessment of Cell Viability 30
5.4.1 Reagents 30
5.4.2 Materials and Equipment 30
5.4.3 Procedure 30
5.5 Working Sheet: Cell Viability and Concentration by Trypan
Blue Dye Exclusion — or Ethidium Bromide/Acridine Orange 31
Suggested Reading 31
Chapter 6
Cell Cryopreservation
6.1 Introduction 33
6.2 Reagents 33
6.3 Materials and Equipment 33
6.4 Procedure 34
6.4.1 Freezing of Cell Cultures 34
6.4.2 Thawing of Cells 34
Suggested Reading 35
Chapter 7
Phagocytosis Functional Assay
7.1 Introduction 37
7.2 Reagents 37
7.3 Materials and Equipment 40
7.4 Procedure 40
7.5 Special Recommendations 42
7.6 Analysis of Results 43
7.7 Species Related Changes in Protocol 44
7.8 Technique Related Changes in Protocol:
Phagocytosis with Yeast 45
7.8.1 Reagents 45
7.8.2 Materials and Equipment 45
7.8.3 Procedure 45
7.9 Statistical Tests 46
7.10 Working Sheet: Phagocytic Function 47
Suggested Reading 48
Chapter 8
Oxidative Burst Assay Using Flow Cytometry
8.1 Introduction 49
8.2 Reagents 49
8.3 Materials and Equipment 50
8.4 Procedure 50
8.5 Special Recommendations 51
8.6 Analysis of Results 54
8.7 Species Related Changes in Protocol 54
8.8 Statistical Tests 54
8.9 Working Sheet: Aquisition List 55
Suggested Reading 56
Chapter 9
Cell Cytotoxicity
9.1 Introduction 57
9.2 Natural Killer Cell Activity 57
9.2.1 Radioactive Chromium 51Cr Assay
(Human or Nonhuman Primates) and DiO (Polar Bear) 57
9.2.1.1 Reagents 57
9.2.1.2 Materials and Equipment 60
9.2.1.3 Procedure 60
9.2.1.4 Special Recommendations 62
9.2.1.5 Analysis of Results 63
9.2.1.6 Expected Results and Statistical Tests 63
9.2.1.7 Species Related Changes in Protocol 64
9.2.2 Flow Cytometric Assay (Polar Bear) 64
9.2.2.1 Reagents 64
9.2.2.2 Materials and Equipment 64
9.2.2.3 Procedure 65
9.2.2.4 Analysis of Results 66
9.2.2.5 Species Related Changes in Protocol 68
9.3 Antibody Dependent Cellular Cytotoxicity 68
9.3.1 Introduction 68
9.3.2 Reagents 70
9.3.3 Materials and Equipment 70
9.3.4 Procedure 70
9.3.5 Analysis of Results and Expected Results 71
9.4 Lymphokine Activated Killer Cells 72
9.4.1 Reagents 72
9.4.2 Materials and Equipment 72
9.4.3 Procedure 72
9.4.4 Analysis of Results 74
9.4.4.1 Data Collection 74
9.4.4.2 Statistical Analysis of the Data 74
9.5 Working Sheet: Acquisition List 75
Suggested Reading 76
Chapter 10
Lymphoblastic Transformation
10.1 Introduction 77
10.2 Reagents 77
10.3 Materials and Equipment 79
10.4 Procedure 79
10.5 Analysis of Results 80
10.6 Special Recommendations 81
10.7 Species Related Changes in Protocol 81
10.8 Working Sheet 83
10.8.1 Mitogenic Assay — Working Sheet 83
10.8.2 Mitogenic Assay — Follow up Sheet 84
10.8.3 Mitogenic Assay — DPM Result Sheet 85
Suggested Reading 86
Chapter 11
Determination of Antibody Producing Cells to a Specific Antigen
11.1 Introduction 87
11.2 Liquid Plaque Forming Cell Assay in Mouse Model 87
11.2.1 Reagents 87
11.2.2 Materials and Equipment 88
11.2.3 Procedure 88
11.2.4 Results 90
11.3 Agar Plaque Forming Cell Assay in Rat Model 90
11.3.1 Reagents 90
11.3.2 Materials and Equipment 91
11.3.3 Procedure 91
11.3.4 Results 92
11.4 Working Sheet: Plaque Forming Cells 93
Suggested Reading 94
Chapter 12
Mixed Lymphocyte Reaction
12.1 Introduction 95
12.2 Reagents 95
12.3 Materials and Equipment 95
12.4 Procedure 97
12.5 Analysis of Results 98
12.6 Special Recommendations 99
12.7 Species Related Changes in Protocol 99
12.8 Working Sheets 100
12.8.1 Mixed Lymphocyte Reaction — Working Sheet 100
12.8.2 Mixed Lymphocyte Reaction — Follow Up Sheet 101
12.8.3 Mixed Lymphocyte Reaction — DPM Result Sheet 102
Suggested Reading 103
Chapter 13
Intracellular Levels of Calcium Assay Using Flow Cytometry
13.1 Introduction 105
13.2 Reagents 105
13.3 Materials and Equipment 106
13.4 Procedure 108
13.5 Special Recommendations 109
13.6 Analysis of Results 110
13.7 Species Related Changes in Protocol Ill
13.8 Statistical Tests 111
13.9 Working Sheet: Acquisition List 112
Suggested Reading 113
Chapter 14
Phenotyping of Blood Mononuclear Cells
14.1 Introduction 115
14.2 Reagents 115
14.3 Materials and Equipment 115
14.4 Procedure 116
14.5 Analysis of Results 117
14.6 Species Related Changes in Protocol 121
14.7 Immunophenotype of Rat Peripheral Blood Lymphocytes 122
14.8 Working Sheets 122
14.8.1 Data List for Antibodies 122
14.8.2 Staining Follow Up Sheet 123
14.8.3 Acquisition List 124
Suggested Reading 125
Chapter IS
Evaluation of Intracellular Level of Thiols
15.1 Introduction 127
15.2 Reagents 127
15.3 Materials and Equipment 127
15.4 Procedure 128
15.5 Analysis of Results 129
15.6 Species Related Changes in Protocol 130
15.7 Working Sheet: Acquisition List 130
Suggested Reading 131
Chapter 16
Apoptosis
16.1 Introduction 133
16.2 Reagents 133
16.3 Materials and Equipment 133
16.4 Procedure 134
16.5 Analysis of Results 135
Suggested Reading 135
Index 137
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| spelling | Manual of immunological methods Pauline Brousseau ... Boca Raton [u.a.] CRC Press 1999 141 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Pharmacology and toxicology Immunodosage - Guides, manuels, etc Immunologie - Technique - Guides, manuels, etc Inmunología - Técnica - Manuales Animals, Laboratory Immunoassay Handbooks, manuals, etc Immunologic Techniques Immunology Technique Handbooks, manuals, etc Immunologie (DE-588)4026637-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Immunassay (DE-588)4113998-7 gnd rswk-swf Immunassay (DE-588)4113998-7 s Methode (DE-588)4038971-6 s DE-604 Immunologie (DE-588)4026637-0 s Brousseau, Pauline Sonstige oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008378624&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Manual of immunological methods Immunodosage - Guides, manuels, etc Immunologie - Technique - Guides, manuels, etc Inmunología - Técnica - Manuales Animals, Laboratory Immunoassay Handbooks, manuals, etc Immunologic Techniques Immunology Technique Handbooks, manuals, etc Immunologie (DE-588)4026637-0 gnd Methode (DE-588)4038971-6 gnd Immunassay (DE-588)4113998-7 gnd |
| subject_GND | (DE-588)4026637-0 (DE-588)4038971-6 (DE-588)4113998-7 |
| title | Manual of immunological methods |
| title_auth | Manual of immunological methods |
| title_exact_search | Manual of immunological methods |
| title_full | Manual of immunological methods Pauline Brousseau ... |
| title_fullStr | Manual of immunological methods Pauline Brousseau ... |
| title_full_unstemmed | Manual of immunological methods Pauline Brousseau ... |
| title_short | Manual of immunological methods |
| title_sort | manual of immunological methods |
| topic | Immunodosage - Guides, manuels, etc Immunologie - Technique - Guides, manuels, etc Inmunología - Técnica - Manuales Animals, Laboratory Immunoassay Handbooks, manuals, etc Immunologic Techniques Immunology Technique Handbooks, manuals, etc Immunologie (DE-588)4026637-0 gnd Methode (DE-588)4038971-6 gnd Immunassay (DE-588)4113998-7 gnd |
| topic_facet | Immunodosage - Guides, manuels, etc Immunologie - Technique - Guides, manuels, etc Inmunología - Técnica - Manuales Animals, Laboratory Immunoassay Handbooks, manuals, etc Immunologic Techniques Immunology Technique Handbooks, manuals, etc Immunologie Methode Immunassay |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=008378624&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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